卵巢癌患者白体细胞因子诱导杀伤细胞治疗前后免疫功能的检测与分析

目的 观察卵巢癌患者自体细胞因子诱导杀伤细胞治疗前后患者外周血淋巴细胞亚群的变化，为综合评价临床应用CIK细胞疗法治疗卵巢癌的效果提供依据。方法 2004—2005采集中国医科大学附属第一医院5例卵巢癌患者的外周血单个核细胞，经多种细胞因子诱导制成CIK细胞，回输前及回输后取患者外周血，流式细胞仪测定外周血淋巴细胞亚群的变化。结果 诱导培养后，CD3^＋／CD56^＋效应细胞的比例明显升高；CIK细胞回输后患者外周血CD3^＋、CD3^＋／CD8^＋、CD3^＋／CD56^＋阳性淋巴细胞亚群的比例明显升高。结论 CIK细胞疗法可以提高卵巢癌患者的细胞免疫功能，提高对肿瘤细胞的杀伤作用，     

强化CIK细胞的培养及治疗中晚期卵巢癌的疗效观察

目的:从患者自体外周血培养强化的细胞因子诱导的杀伤细胞(CIK),回输治疗9例术后或放化疗后的中晚期卵巢癌并观察疗效。方法:使用瓶壁包被抗CD3和抗CD28抗体,并外加IL-2和IL-15等方法,建立强化CIK培养体系,观察其增殖活性、功能基因表达和体外杀瘤效果。选取9例中晚期卵巢癌患者,分别进行至少3个疗程的强化CIK细胞静脉回输治疗,观察患者短期治疗效果、KPS变化、淋巴细胞亚群变化及相应的不良反应。结果:培养的强化CIK细胞增殖活性高,功能基因表达和体外杀伤卵巢腺癌细胞株SKOV3的能力均高于普通CIK方法;9例患者经强化CIK治疗后,CR 1例,PR 2例,SD 4例,PD 2例,总有效率达78%;KPS评分增加≥20分4例(44%),疼痛减轻≥50%4例(44%);治疗期间1例出现发热反应(T38.5℃),1例出现皮疹伴瘙痒。结论:在放化疗降低肿瘤负荷,改善肿瘤微环境后,多次回输强化培养的CIK可有效杀伤体内残留肿瘤组织,改善中晚期妇科肿瘤患者的生存质量。     

卵巢肿瘤患者外周血中T细胞亚群及调节T细胞含量变化的临床意义

目的 探讨T淋巴细胞亚群及调节T细胞含量变化在卵巢肿瘤评价中的临床意义.方法 应用流式细胞仪检测了155例卵巢恶性肿瘤患者及100例卵巢良性肿瘤患者及50例健康人群外周血T淋巴细胞亚群及调节T细胞含量.结果 与健康对照(1.81±0.45)相比,卵巢癌患者外周血中CD4/CD8比值明显降低(0.91±0.31),二者差异有统计学意义(P＜0.01),CD4/CD8比值随卵巢癌分级的升高而下降,但卵巢癌组间差异无统计学意义(P＞0.05).与健康对照组(4.67±1.84)％及卵巢良性肿瘤(5.32±0.83)％相比,卵巢癌患者外周血中Treg比例明显增加(11.86±2.41)％,差异有统计学意义(P＜0.01),Treg比例随卵巢癌分级的升高而升高,卵巢癌组间差异无统计学意义(P＞0.05).结论 卵巢癌患者外周血CD4/CD8比值明显降低,而CD4+ CD25+ Treg细胞水平明显升高,并且与肿瘤的分级有关,可能提示卵巢癌患者机体免疫杀伤功能低下而免疫耐受增加,从而使肿瘤发生免疫逃逸,促使肿瘤的发生发展.     

卵巢癌患者腹水来源树突状细胞强化CIK细胞对卵巢癌细胞的杀伤作用

目的:建立诱导卵巢癌患者腹水来源的树突状细胞(DC)的方法,并观察腹水DC对CIK细胞在体外杀伤卵巢癌细胞系SKOV3的作用。方法:分离腹水单核细胞后诱导DC,分离患者外周血单个核细胞诱生CIK细胞,通过流式细胞仪分析免疫表型,并以LDH释放法检测DC对CIK细胞在体外杀伤卵巢癌细胞株的作用。结果:除外周血单核细胞来源DC表达CD86较高外,其他表面分子及异基因刺激能力在不同来源的DC间没有差异。负载SKOV3抗原的DC能诱导出CIK细胞对SKOV3细胞系最强的细胞毒杀伤力,负载HO8910的DC与单纯DC次之,且两者无差异。CIK组细胞毒性最低。结论:卵巢癌患者腹水中含有大量的免疫活性细胞,其中更有丰富的DC前体细胞,可诱导出成熟有功能的DC。冻融肿瘤细胞获取全细胞抗原法可以在不明确肿瘤特异抗原的情况下使用,负载于DC后可以诱导出CIK细胞的特异性杀伤。     

免疫治疗原因不明习惯性流产后外周血T淋巴细胞亚群的变化

目的:探讨原因不明习惯性流产患者外周血T淋巴细胞亚群在主动和被动免疫治疗前后的变化。方法:采用流式细胞仪对2 5例被动免疫治疗和38例主动免疫治疗的原因不明性习惯性流产患者作治疗前后外周血T淋巴细胞亚群含量对比,并选择2 0例正常妊娠者作对照组。结果:①被动与主动免疫组治疗前后CD3+细胞无明显差异。②被动和主动组治疗后CD4 +细胞较治疗前有明显减少。③CD8+细胞在被动免疫治疗和主动免疫治疗后较治疗前有明显升高。④CD4 +/CD8+比率在被动免疫治疗和主动免疫治疗后较治疗前明显下降。结论:主动和被动免疫治疗后,外周血T淋巴细胞亚群有了明显变化,更利于妊娠发展。     

妊娠期糖尿病患者母儿免疫水平变化的临床研究

目的:研究妊娠期糖尿病(GDM)患者母儿免疫球蛋白(Ig)、补体(C)、T淋巴细胞亚群以及NK细胞水平的变化,探讨GDM对母儿体液免疫和细胞免疫的影响。方法:选择58例不同糖耐量水平的GDM患者为研究对象根据是否需要胰岛素治疗进一步分为GDM1组和GDM2组,以30例健康孕妇做对照。免疫比浊法测定外周静脉血与脐血的免疫球蛋白、补体水平;流式细胞技术测定外周静脉血与脐血的T细胞亚群及NK细胞水平。结果:GDM组外周血CD4+、CD4+/CD8+、CD16+CD56+、IgG含量均下降,CD8+、C3、C4、IgE含量升高,差异有统计意义(P<0.05);CD3+、IgA、IgM含量下降,差异无统计学意义(P>0.05)。GDM组脐血中CD3+、CD4+、CD4+/CD8+、CD16+CD56+含量均下降,差异有统计学意义(P<0.05);IgM、IgG含量下降,CD8+、IgE含量升高,差异无统计学意义(P>0.05)。与GDM1组相比,GDM2的指标呈现更明显的变化趋势。结论:妊娠期糖尿病存在母儿体液免疫和细胞免疫的失衡,且病情越重,这种改变越显著。     

原因不明复发性流产患者淋巴细胞主动免疫治疗前后细胞亚群的变化

目的探讨淋巴细胞主动免疫治疗对原因不明复发性流产的疗效及在主动免疫治疗前后患者微量淋巴细胞毒实验淋巴细胞亚群的变化。方法应用荧光染色法和流式细胞术,分别对2009年4月至2010年12月在安徽医科大学第一附属医院行主动免疫治疗的48例原因不明复发性流产患者治疗前后的淋巴细胞坏死率和CD3、CD4、CD8及CD16+CD56+NK细胞的比率进行测定;同时随访其预后。结果 43例成功妊娠至孕12周之后,5例患者在孕12周前流产,主动免疫治疗成功率89.6%(43/48)。治疗后患者淋巴细胞坏死率上升,CD3、CD4和CD16+CD56+NK亚群比率下降,CD8亚群比率上升,差异有统计学意义(P<0.05,P<0.01)。结论淋巴细胞主动免疫治疗对原因不明复发性流产治疗效果满意,CD8亚群的升高可能有利于妊娠。     

外周血淋巴细胞亚群检测对儿童亚急性坏死性淋巴结炎的诊疗意义

目的探讨外周血淋巴细胞亚群检测对儿童亚急性坏死性淋巴结炎(CSNL)的诊疗意义。方法选取2013年1月至2016年12月河南省人民医院小儿外科及内科收治的CSNL患儿51例为CSNL组,同期选择门诊健康体检儿童30例为对照组。采用流式细胞技术检测两组受试对象外周血T淋巴细胞亚群百分率,并观察CSNL治疗效果。结果 51例CSNL患儿中,治愈38例,好转12例,无效1例。治疗前CSNL患儿CD3~+、CD4~+、CD19~+淋巴细胞亚群百分率和CD4~+/CD8~+均显著低于对照组,差异有统计学意义(P0.05)。38例治愈患儿和12例好转患儿治疗中、后外周血CD3~+、CD4~+、CD19~+淋巴细胞亚群百分率和CD4~+/CD8~+显著高于治疗前,治疗后的指标又显著高于治疗中,差异有统计学意义(P0.05)。结论细胞亚群CD3~+、CD4~+、CD4~+/CD8~+及CD19~+,可作为临床CSNL诊疗的重要评估指标。     

复发性流产和种植失败妇女外周血自然杀伤细胞亚群的天然细胞毒性受体及a2V-ATPase的表达

自然杀伤(NK)细胞在人类妊娠过程中发挥着重要作用,占外周血淋巴细胞的5%～10%。NK细胞与其他细胞区别的特征性表面标志是CD56 。人类NK细胞亚群不是表达CD56bright、就是表达CD56dim。在外周血中主要是NKT细胞,在蜕膜和胎盘部位主要是CD56bright细胞。     

卵巢癌SKOV3细胞培养液对脐血树突细胞前体细胞亚群的影响

目的:研究卵巢癌细胞培养上清能否诱导树突细胞前体细胞亚群比例发生变化。方法:以脐血来源的CD34+干细胞通过细胞因子FLT3-L(100ng/ml)、SCF(50ng/ml)的诱导在体外扩增分化为DC前体细胞(pre-DC)。培养并收集卵巢癌细胞株SKOV3上清,经透析法浓缩。将上清培养的pre-DC,经不同浓度上清(0%、25%、50%、75%、100%、150%)作用后,用流式细胞仪(flowcytometry,FCM)检测pre-DC表面抗原CD123、CD11c、HLA-DR、CD80、CD86的表达。结果:上清促进CD11c和HLA-DR抗原的表达,不促进CD123、CD80、CD86抗原的表达。结论:卵巢癌细胞株上清影响两类DC前体细胞亚群的比例变化,可能通过此途径参与形成腹腔免疫缺陷。     

Significant differences of lymphocytes isolated from ascites of patients with ovarian cancer compared to blood and tumor lymphocytes. Association of CD3+CD56+ cells with platinum resistance

OBJECTIVES: Tumor infiltrating lymphocytes (TILs) and T regulatory cells (Tregs) have been associated with prognosis in ovarian cancer, but their prognostic significance in ascites has not been studied. We performed a prospective study of T lymphocytes isolated from ascites from patients with ovarian carcinoma and we compared them with the respective populations in blood and tumors. METHODS: Mononuclear cells from ascites (n=71) and blood were isolated by Ficoll, while tumor lymphocytes (n=20) were obtained upon mechanical dissociation. Phenotypic analysis was performed with flow cytometry. Ascites from 10 patients with cirrhosis was used as control. RESULTS: Tregs containing CD4(+)CD25(+) cells, NK-T containing CD3(+)CD56(+) cells and CD69 and HLADR expression of CD4 and CD8 lymphocytes were significantly increased in tumor ascites compared to blood and control ascites. A selective accumulation of these populations in the ascites of cancer patients, was suggested by the significantly higher ascites/blood (A/B) ratios in cancer patients but not controls. Cancer cell content in ascites was correlated with CD4(+)CD25(+), CD4(+)CD69(+), CD4(+)HLADR(+) and CD8(+)CD69(+) cells. There was no correlation of lymphocyte populations between ascites and samples from peritoneal metastases. Higher tumor grade was associated with increased A/B CD4(+)CD25(+) ratio and reduced CD3(+)CD56(+) cells, while platinum resistance was associated with reduced A/B CD3(+)CD56(+) ratio. CONCLUSIONS: There are significant differences of CD3(+)CD56(+) and CD25(+)CD4(+) lymphocytes and increase in lymphocyte activation between blood, ascites and peritoneal metastases from patients with ovarian cancer. The selective accumulation of CD3(+)CD56(+) population in ascites may be a predictive factor for platinum resistance.     

Effect of dexamethasone on lymphocyte subpopulations in premature infants with bronchopulmonary dysplasia.

OBJECTIVE: This study was designed to determine the effect of dexamethasone treatment on peripheral blood lymphocyte counts and subpopulations in premature infants with bronchopulmonary dysplasia (BPD). STUDY DESIGN: Peripheral blood lymphocyte subpopulations in 12 premature infants with BPD were analyzed before treatment with a 6-week course of dexamethasone (day 0), on days 3 and 10 of treatment, and 2 weeks after discontinuing dexamethasone therapy (day 56). Lymphocyte immunophenotypes were determined using direct two-color immunofluorescent staining followed by flow cytometry. RESULTS: The percentage of lymphocytes was significantly lower on days 3 (17.55 +/- 2.55) and 10 (20 +/- 11.8) of dexamethasone therapy compared with before (30.36 +/- 6.41) or after treatment. The percentage of T cells was significantly lower on days 3 and 10 of dexamethasone therapy (mean +/- SEM; 58.09 +/- 1.93 and 60.09 +/- 2.47, respectively) compared with before (67.09 +/- 4.24) or after treatment. The absolute number of T cells was significantly lower on day 10 of therapy. The percentage of CD4+ cells was significantly lower on days 3 (38.91 +/- 2.49) and 10 (40.45 +/- 2.24) of therapy, and this decrease persisted after dexamethasone was stopped (36.73 +/- 3.41). The absolute number of CD4 cells was significantly lower on day 10 (1328 +/- 216) of therapy and reached a nadir on day 56 (1143 +/- 106). Similarly, the CD4/CD8 ratio was also significantly lower on days 3 and 10 of treatment (1.56 +/- 0.18 and 1.64 +/- 0.14, respectively) and reached a nadir on day 56 (1.04 +/- 0.13). CONCLUSION: Dexamethasone significantly reduced the percentage and absolute number of lymphocytes, T cells, and CD4 cells, as well as the CD4/CD8 ratio. A reduction in CD4 cells and in the CD4/CD8 ratio persisted 2 weeks after dexamethasone therapy was stopped. In contrast, the absolute number of B cells increased transiently, and CD8 cells were unaffected by dexamethasone. This alteration in lymphocyte subpopulations may help account for the clinically beneficial anti-inflammatory effect of dexamethasone in the treatment of BPD complicated by respiratory failure. The dexamethasone-induced decrease in CD4 cells may also increase the susceptibility of these infants to infection.     

Pregnancy after four recurrent pregnancy losses: a case report

The patient M.S, 24 years old has been admitted to the Department of Obstetrics and Perinatology of University School of Medicine in Lublin with diagnosis: V pregnancy, bronchial asthma. History taken from the patient revealed four recurrent pregnancy losses. The patient has taken prednisone in the dose 10 mg per day for several weeks. After performing immunological phenotyping of lymphocytes we have found some alterations in the patient's immune status: increased T CD4:TCD8 ratio, increased percentage of B CD19+ and B CD19+5+ lymphocytes, increased percentage of Natural Killer cells CD3(-)16/56+, deficiency of T CD8+ suppressor lymphocytes and increased expression of CD25 and HLA-DR antigens on T CD4+ lymphocytes. According to the obtained results we have increased prednisone dose to 20 mg per day. After forty days of prednisone therapy in the dose mentioned above patient's immunological status has evolution favorably: T CD4:TCD8 ratio decreased, percentage of B CD19+ and B CD19+5+ lymphocytes decreased, percentage of Natural Killer cells CD3(-)16/56+ decreased, the expression of CD25 and HLA-DR antigens on T CD4+ lymphocytes decreased. Due to the PROM (premature rupture of membranes), and obstetric anamnesis the patient was qualified for caesarean section (21st of September 1998). Male baby was born in good general condition (weight 1180 g, Apgar score 8 pts.). The baby was discharged from Prematurity Department 10th of November 1998 in good general status (weight 2180 g). The child was observed for one year after being discharged from the ward, psycho-physical development is normal.     

Expression of inhibitory natural killer receptors on tumor-infiltrating CD8+ T lymphocyte lineage in human endometrial carcinoma

To investigate the expression of natural killer receptors (NKRs) within the human tumor milieu, we directly examined the in vivo expressions of various NKRs on tumor-infiltrating lymphocytes (TILs) derived from human endometrial carcinoma (EC). In total, 22 patients with stage IA-IIIA EC were enrolled. TILs were isolated from tissue specimens by means of a mechanical dispersal technique. The subpopulations of immunocytes were quantified, and expressions of NKRs on CD8+ T cells were analyzed by triple-color flow cytometry. CD8+ T cells express higher ratios of CD94 and NKG2A in TILs than in peripheral blood mononuclear cells (PBMCs) in human EC. Flow cytometry reveals that 15.90% of CD3+CD8+ TILs compared with 2.10% of CD3+CD8+ PBMCs express the NKG2A molecules (P < 0.001). The percentage expressions of CD94 are 8.40% in CD3+CD8+ TILs and 3.80% in CD3+CD8+ PBMCs (P= 0.013). The numbers of CD8+ T cells expressing CD158b and NKB1 are higher in CD3+CD8+ PBMCs in EC than in normal (CD158b: 10.70% vs 2.60%, P < 0.001; NKB1: 2.20% vs 0.40%, P= 0.018, respectively). Increased expression of CD94/NKG2A restricted to tumor-infiltrating CD8+ T cell subsets may shape the cytotoxic responses, which indicate a possible role of tumor escape from host immunity in human EC.     

葡萄球菌肠毒素激活肿瘤浸润淋巴细胞及外周血淋巴细胞体外抗人卵巢癌的对比研究

目的:探讨葡萄球菌肠毒素A(SEA)对卵巢癌肿瘤浸润淋巴细胞(TIL)及其外周血淋巴细胞(PBL)抗瘤活性的诱导作用。方法:取10例卵巢癌伴腹水患者实体瘤、腹水及外周血标本,分离TIL和PBL。在SEA及IL-2作用下培养,定时计数,了解其增殖情况;流式细胞仪检测其CD3、CD4、CD8表达;噻唑蓝(MTT)比色法测定其对K562及自体肿瘤细胞的细胞毒活性;酶联免疫吸附试验(ELISA)测定培养上清液中TNF-a和IFN-γ浓度。结果:10例中8例成功分离实体瘤TIL、腹水TIL及PBL。(1)SEA刺激的实体瘤TIL、腹水TIL及PBL增殖速率明显较IL-2诱导组快(P<0.05),但增殖高峰后出现下降趋势,IL-2组未出现此现象;(2)CD3+CD4+及CD3+CD8+T表达率均明显上升,其中SEA诱导组比IL-2组增加比例明显(P<0.05),以SEA作用的CD3+CD8+T比例增加最快;(3)TIL对自体肿瘤细胞的杀伤活性明显高于对K562细胞的杀伤活性(P<0.05),PBL对自体肿瘤细胞的杀伤活性则明显低于对K562细胞的杀伤活性(P<0.05),SEA激活组比IL-2组杀伤率高(P<0.05);(4)各效应细胞分泌的TNF-a、IFN-γ分别在培养的第2天和第4天达到高峰,高峰后迅速下降,SEA诱导组在前10天明显高于IL-2诱导组(P<0.05)。结论:SEA可高效、迅速诱导卵巢癌TIL的抗瘤活性。     

盆腔子宫内膜异位症患者NK细胞表面自然细胞毒受体表达的研究

目的:探讨盆腔子宫内膜异位症患者外周血和腹腔液NK细胞表面自然细胞毒受体的表达及意义。方法:用流式细胞术直标法检测20例盆腔子宫内膜异位症患者和13例非子宫内膜异位症对照者外周血和腹腔液NK细胞表面自然细胞毒受体NKp30、NKp44和NKp46的表达。结果:(1)NKp30在腹腔液CD56+NK细胞表面表达观察组较对照组明显减少(P<0.05),在腹腔液CD16+NK细胞表面和外周血CD56+/CD16+NK细胞表面表达两组之间则无差异;NKp46在外周血和腹腔液CD56+/CD16+NK细胞表面表达观察组和对照组之间无显著差异;NKp44在外周血和腹腔液CD56+/CD16+NK细胞表面均无表达;(2)NKp30在腹腔液CD56+CD16+NK细胞上表达观察组比对照组明显减少;外周血CD56+CD16+NK细胞表面NKp30表达以及外周血和腹腔液CD56+CD16+NK细胞表面NKp46表达观察组和对照组之间则无差异;(3)NKp30和NKp46在腹腔液CD16+NK细胞表面表达较外周血显著降低(PNKp30<0.05;PNKp46<0.01),而CD56+NK细胞表面外周血和腹腔液之间的表达则无差异。结论:腹腔液NK细胞表面自然细胞毒受体NKp30在盆腔子宫内膜异位症发病过程中起作用。     

Decidual lymphocyte subsets in pregnant women

OBJECTIVES: Materno-foetal immunological reactions in decidua are probably one of the most important elements in pathogenesis of preeclampsia. DESIGN: To compare lymphocyte subsets isolated from decidua of preeclamptic pregnant women with lymphocyte subsets isolated from healthy pregnant women. MATERIALS AND METHODS: Preeclampsia (PE) was defined according to USA National Health Institute criteria. The study group consisted of 21 women with PE and 11 women with physiological pregnancy. All pregnancies were finished with elective cesarean sections. Exclusion criteria were: uterine contractions, infection, chorinamnionitis, diabetes mellitus and therapy with steroids less than 7 days before blood sampling. Decidual tissue was obtained by curettage of the uterine cavity. The fragments of decidua were separated from clotted blood and placed in sterile tubes with 5 ml of isotonic solution (PBS). Then the decidual tissue was mechanically fragmented, homogenized and rinsed in PBS. Routine immunofluorescent marking techniques with monoclonal antibodies were performed. Analysis was done with FACSCalibur flow-cytometer with 488 nm argon laser using CellQuest programme. The following lymphocyte subsets were estimated: CD3, CD19, CD4, CD8, CD4/CD29, CD8/CD28, CD4/CD45RA, CD4/CD45RO, CD56/CD16, CD69. The results were described as percentage of lymphocytes positive for above surface molecules. Statistical analysis was performed using t-Student and U-Mann-Whitney tests. The work was sponsored by KBN 4 P05E 118 15 grant. RESULTS: Decidua of pregnant PE women contains significantly increased percentage of CD3-/ CD56 + 16+, CD8+/CD28+ cells and decreased percentage of CD3+, CD19+, CD4+/CD29+ and CD4+/CD45RA+ compared to decidua of healthy pregnant controls. CONCLUSIONS: These changes suggest that deficiency of suppressor activity as well as aberrant immunoregulation exists in decidual tissue of PE women.     

The role of peripheral blood lymphocyte subpopulations in differentiation between preeclampsia and transient hypertension

OBJECTIVES: Hypertension is the most frequent complication of pregnancy after 24th week of gestation, occurring in 8% of pregnancies and being the main cause of perinatal mortality and morbidity. It is classified as preeclampsia (PE) or transient hypertension (TH). According to some statements PE and TH are distinct syndromes of different pathogenesis. There are even opinions emphasizing that in most cases TH is in fact undiagnosed chronic hypertension. The role of immunological system in pathogenesis of PE is well known but the hypothesis that immunological events are engaged in pathogenesis of chronic hypertension has not been proved so far. Assuming that TH is closer in its pathogenesis to chronic hypertension than to PE it would be possible to differentiate between TH and PE using some immunological tests. If PE and TH are the same, the differences would be insignificant. DESIGN: The aim of this study was to check the hypothesis that peripheral blood lymphocyte subsets analysis is an useful tool in differentiation between PE and TH and confirmation of their distinct origin. MATERIALS AND METHODS: The study groups consisted of 19 pregnant women with PE (mean age 25.5 +/- 2.5 years, mean gestational age 32.5 +/- 2.5 weeks, 84.2% primiparae) and 14 pregnant women with TH (mean age 27.0 +/- 3.0 years, mean gestational age 33.5 +/- 3.0 weeks, 100% primiparae) diagnosed between 30-37 week of gestation. All women were matched according to gestational age and race. They had no renal diseases or chronic hypertension prior to pregnancy neither had any features of them during the study. Exclusion criteria were: uterine contractions, infection and therapy with steroids before blood sampling. PE and TH were defined according to USA National Health Institute criteria. Peripheral blood was obtained by venipuncture. Standard immunofluorescent marking techniques for whole blood with one-step monoclonal antibodies were performed. Lymphocyte subsets (CD19+, CD3+, CD4+, CD8+, CD3-/CD16+/CD56+, CD3+/CD16+/CD56+, CD8+/CD28+, CD4+/CD45RA+, CD4+/CD45RO+, CD3+/CD69+) analysis was done with flow-cytometer FACSCalibur with 488 nm argon laser. The lymphocyte cells region was chosen with LeucoGATE and analysis performed with SimulSET v.3.1 programme. Statistical analysis was based on Student T test. RESULTS: The differences in peripheral blood lymphocyte subsets composition between PE and TH were insignificant. CONCLUSION: Is that on the basis of peripheral blood lymphocyte subsets analysis PE and TH despite different clinical symptoms seem to have common pathogenesis. However there is possibility that changes observed in peripheral blood are not significantly different in PE and TH because of their low importance for immunopathogenesis.