共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
Adenovirus-mediated E2F-1 gene transfer in nonsmall-cell lung cancer induces cell growth arrest and apoptosis. 总被引:3,自引:0,他引:3
H Kuhn U Liebers C Gessner A Schumacher C Witt J Schauer I Kovesdi G Wolff 《The European respiratory journal》2002,20(3):703-709
Since overexpression of E2F-1 has been shown to induce apoptosis, the ability of adenovirus-mediated transfer of E2F-1 to inhibit tumour growth in nonsmall-cell lung cancer cell lines was investigated. Three cell lines with various genomic status were infected with AdE2F. Cell proliferation and viability were determined by trypan blue exclusion. Apoptosis induction was assessed by flow cytometry and poly-adenosine diphosphate-ribose-polymerase cleavage assay. In vivo, the effect of E2F-1 on tumour growth was determined in severe combined immunodeficiency (SCID) mice. The current experiments showed that overexpression of E2F-1 suppressed tumour cell growth. The population of apoptotic cells was dramatically increased 96 h after infection with AdE2F. Inhibition of cell growth and induction of apoptosis was not dependent on genomic status. Moreover, treatment of implanted tumours in SCID mice with AdE2F inhibited tumour growth. These data suggest that adenovirus-mediated E2F-1 gene therapy may be effective in the treatment of nonsmall-cell lung cancer. 相似文献
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
Over-expression of E2F-1 in esophageal squamous cell carcinoma correlates with tumor progression 总被引:5,自引:0,他引:5
Y. Ebihara M. Miyamoto T. Shichinohe Y. Kawarada Y. Cho A. Fukunaga S. Murakami H. Uehara H. Kaneko H. Hashimoto Y. Murakami T. Itoh S. Okushiba S. Kondo H. Katoh 《Diseases of the esophagus》2004,17(2):150-154
15.
16.
17.
18.
19.
20.
Endogenous E2F-1 promotes timely G0 exit of resting mouse embryo fibroblasts 总被引:7,自引:0,他引:7
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Zhiyan M. Wang Hong Yang David M. Livingston 《Proceedings of the National Academy of Sciences of the United States of America》1998,95(26):15583-15586
Much evidence strongly suggests a positive role for one or more E2F species in the control of exit from G0/G1. Results described here provide direct evidence that endogenous E2F-1, as predicted, contributes to progression from G0 to S. By contrast, cycling cells lacking an intact E2F-1 gene demonstrated normal cell cycle distribution. Therefore, E2F-1 exerts a unique function leading to timely G0 exit of resting cultured primary cells, while at the same time being unnecessary for normal G1 to S phase progression of cycling cells. 相似文献