BALB/c小鼠乳腺癌细胞系(MA-901C)的建立及部分生物学特性

MA—901C是一株BALB/c小鼠自发性乳腺癌细胞系,来源于B型乳腺癌MA—901,已传31代。细胞有圆形、梭形两种,呈单层鳞状样排列,当持续培养时,形成“帽状”、“瓶底样”的三维结构,细胞倍增时间为23.5h,最高分裂指数为58.59‰,染色体数目为70～78,众数为72,同种动物体内移植成功(3/3),其肿瘤形态和组织结构仍保持原发肿瘤的生物学特性。     

LRH-A对可移植性小鼠乳腺癌(MA-737)生长组化观察

用可移植性乳腺癌(MA-737)瘤株接种36只小鼠,平均分成三组:对照组、LRH-A组和5Fu组,于13天和20天分两批处死。观察瘤组织的生长、组织化学和测量瘤组织重量。观察到LRH-A对瘤组织增重有一定的抑制作用。对照组乳腺癌细胞内AKP、ACP、LDH、SDH和DNA均比其余两组明显增高。说明LRH-A对小鼠乳癌生长有一定抑制作用。     

BALB／c-nu／＋小鼠可移植性自发乳腺癌的研究

将一例BALB／c-nu／ 小鼠自发乳腺癌移植于同品系小鼠皮下，一年间共传代24代，成功率达100%，无自发消退现象，该移植癌潜伏期短，生长稳定，免疫组织化学检查均为上皮来源，经光学显微镜检查各代移植瘤形意结构与原代组织结构基本一致．电镜检查细胞搭列不规则，胞间可见桥粒，胞质内线粒体、内质网、高尔基复合体丰富，也可见张力原纤维，分泌颗粒。核仁明显，异染色质边集。     

BALB/c突变卷毛小鼠血液学指标的检测分析

目的探讨BALB/c突变卷毛小鼠的血液学指标与正常BALB/c小鼠之间是否存在突变差异。方法选择6周龄的BALB/c突变卷毛小鼠和正常BALB/c小鼠各20只(雌雄各半),使用全自动血细胞分析仪检测8项血液常规指标;使用全自动生化仪检测15项血清电解质及生化指标,并对两组结果进行对比分析。结果 BALB/c突变卷毛小鼠的白细胞、平均血细胞容积、血小板计数和平均血细胞血红蛋白浓度的性别及组间结果与正常小鼠具有显著性差异(P0.05,P0.01);BALB/c突变卷毛小鼠Na+、K+和Cl-的性别及组间结果与正常小鼠具有显著性差异(P0.05,P0.01);BALB/c突变卷毛小鼠丙氨酸转氨酶、葡萄糖和甘油三酯的性别和组间结果与正常小鼠具有显著性差异(P0.05,P0.01)。结论 BALB/c突变卷毛小鼠和正常BALB/c小鼠的部分血液学指标存在明显差异,这为今后研究和应用BALB/c突变卷毛小鼠模型提供了理论参考。     

不同年龄BALB/c小鼠乳糖酶及基因调控水平研究

目的 通过测定不同年龄段小鼠乳糖酶活性和乳糖酶mRNA水平,探讨乳糖酶变化趋势及其基因调节途径。方法 分别选取3、5、7、9周龄的BALB／c小鼠,测定其乳糖酶活性及其mRNA水平。乳糖酶活性测定采用Dahlqvist改良法,mRNA水平采用原位杂交,半定量分析。结果 小鼠3周龄时乳糖酶活性最高,与其他各组有显著差异;9周龄小鼠乳糖酶活性最低,与其他三组有显著差异。而乳糖酶mRNA水平无差异。结论 结果提示断乳前小鼠乳糖酶活性有较高水平,断乳后乳糖酶活性显著降低,说明BALB／c小鼠也存在成年乳糖不耐受问题。各组乳糖酶mRNA水平无统计学差异,提示乳糖酶基因的调控应该是发生在翻译和／或后翻译水平,而并非是在转录水平。     

BALB/c突变卷毛小鼠皮肤组织结构的分析

目的探讨BALB/c突变卷毛小鼠的皮肤组织结构与正常BALB/c小鼠之间是否存在突变差异。方法选择10 d、21 d、42 d和63 d四个不同日龄组的BALB/c突变卷毛小鼠和正常BALB/c小鼠每组各10只,观察两组小鼠被毛外观差异情况,取两小块皮肤组织,直接镜检毛囊结构和皮肤组织病理学检测。结果 BALB/c突变卷毛小鼠生长至10 d时被毛弯曲,能够与同日龄的正常小鼠加以鉴别。镜检观察21 d、42 d和63 d的BALB/c突变卷毛小鼠毛囊中毛发根数明显少于正常小鼠,被毛稀疏且毛发卷曲明显。皮肤组织病理学检测结果显示四个不同日龄组的BALB/c突变卷毛小鼠的毛囊数均显著少于正常小鼠。63 d的BALB/c突变卷毛小鼠处于生长期的毛囊中可见毛发弯曲的现象。结论 BALB/c突变卷毛小鼠的毛囊和皮肤组织结构与正常小鼠存在明显差异,说明其皮肤组织结构也发生了改变,这为今后研究突变卷毛小鼠的突变基因和模型应用提供了理论参考。     

BALB/c小鼠可移植性乳腺癌(MA-901)动物模型的建立及其组织病理学研究

该模型用本所发现的BALB/c小鼠自发性乳腺癌作瘤源,同源小鼠作宿主,连续皮下移植传代,目前已达21代。从移植第一代起,即出现自发肺、肝转移,其转移比数为3/3。移植第9代时,曾作连续动态病理切片观察,移植后第11天即可见肺转移,肝转移多发生在20天左右,90天以后肺、肝转移率均高达100%,这在可移植性小鼠肿瘤模型中罕见。晚期亦可见脾、肾转移。该肿瘤以血道转移为主,亦可见淋巴转移。从移植肿瘤后第11天开始,宿主脾脏进行性肿大,形成巨脾症亦是本模型的重要特征之一。     

风疹病毒在BALB/c小鼠体内的感染与分布

用BALB/c小鼠形成风疹病毒感染模型,小鼠经尾静脉感染RVGOS_(-10)株10~6TCID_(50),每只0.5ml,病毒血症持续约一周,感染后第4天达最高滴度,为10~(6.3)TCID_(50)/0.25ml,间接免疫荧光及间接酶标染色法观察小鼠组织切片,感染后不同时间,病毒对小鼠各脏器可形成广泛的侵害,抗原主要分布于胞桨,病毒分离结果与之吻合,感染后第18天,心、眼、肺中的病毒滴度分别为10~(5.2),10~(5.6),10~(5.5)TCID_(50)。     

BALB/c 小鼠小肠乳糖酶活性双糖酶法测定条件的优化

目的建立一种方便、准确测定小鼠小肠乳糖酶活性的方法.方法采用不同pH的底物溶液与肠黏膜提取物反应,计算相应的酶活性;取不同反应时间(45 min,60 min,75 min),不同显色时间(45 min,60 min,75 min)和不同显色剂用量(2.0 ml,2.5 ml,3.0 ml)按L9(34)正交实验设计方法,测定不同条件下的底物溶液与肠黏膜反应的A值作为正交实验的评分标准.结果与结论由正交实验得出,该方法的最佳反应条件为pH 4.6,水浴75min,显色时间45 min,显色剂用量3.0 ml;批内酶活性(0.029±0.003)U/ml,CV=2.49%,批间酶活性(0.032±0.004)U/ml,CV=4.19%.该方法具有很好的重复性和可靠性.     

BALB/c突变卷毛小鼠部分生长发育指标初探

目的利用近交培育建立BALB／c突变卷毛小鼠模型,分析突变小鼠与正常小鼠间生长发育和脏器系数的突变差异。方法选择21d、42d和63d三个不同日龄组的BALB／c突变卷毛小鼠和正常BALB／c小鼠各20只,雌雄各半,分别测量体质量、头长、体长、尾长及主要脏器质量,计算头体比、尾体比及脏器系数并进行对比分析。结果F4代的BALB／c突变卷毛小鼠已完全纯合。21d和42d突变卷毛小鼠头体比和尾体比小于正常小鼠（P〈0．05）;63d突变卷毛小鼠的体质量、头体比和尾体比均小于正常小鼠（P〈0．05）。21d突变卷毛小鼠的心脏、脾脏、卵巢、子宫系数均大于正常小鼠（P〈0．05,P〈0．01）;42d突变卷毛小鼠的心脏和胸腺系数低于正常小鼠,脑和睾丸系数高于正常小鼠（P〈0．05,P〈0．01）;63d突变卷毛小鼠的心脏和子宫系数低于正常小鼠,脑系数高于正常小鼠（P〈0．05,P〈0．01）。结论BALB／c突变卷毛小鼠与正常小鼠的外观特征和脏器系数均存在差异,这些突变差异对于研究BALB／c突变卷毛小鼠的生长发育、心血管系统、免疫系统和生殖系统都具有十分重要的意义。     

BALB／c小鼠维生素D慢性中毒的实验研究

目的通过对实验动物大、小鼠饲料配方中维生素D含量进行研究,掌握实验动物大小鼠维生素D中毒的诊断方法.方法将两种不同维生素D含量的饲料作为实验组和对照组进行BALB/c小鼠饲喂实验,对其作病理剖检及血磷、血钙值测定.结果实验组出现心肌钙盐沉积率为53.3%,对照组为0.前者血钙值是后者的1.6倍(P<0.01),血磷值差异无显著性(P>0.05).结论维生素D摄入过量可引起实验动物大、小鼠离乳率下降、心肌钙盐沉积、血钙值升高.     

Immunotoxicity of Acrylamide in Female BALB/c Mice

Objective To investigate the immunotoxicity of acrylamide （ACR） in female BALB/c mice.
Methods A total of 200 female mice weighing 18-22 g were randomly divided into four clusters based on body weight, and each weight-based cluster included five groups （10 mice per group）： negative control, positive control （cyclophosphamide）, low, intermediate, and high dose ACR groups, and all the groups were administered ACR by gavage for 30 days. At the end of the study, the immunotoxicological effects of the ACR were evaluated through immunopathology, humoral immunity, cellular immunity, and non-specific immunity.
Results The terminal body weight, spleen and thymus weights, lymphocyte counts in the ACR-H group were decreased, pathological changes were observed in lymph glands, thymus and spleen. %T cells in blood lymphocytes were significantly increased in all ACR-treated groups, and a significant reduction of % natural killer（NK） cells and increase of %Th cells were observed in the ACR-H group. interleukin-6（IL-6）,
Concanavalin A（ConA）-induced splenocyte proliferation and serum half hemolysis value （HCso） were also significantly suppressed in the ACR-H group. Conclusion ACR elicited an inhibitory effect on cellular and humoral immunity of mice after 30 day feeding.     

BALB/c小鼠乳糖酶基因Lac的克隆及其cDNA探针的制备

目的 提取、克隆BALB／c小鼠乳糖酶基因Lac,了解其序列及所编码蛋白的属性,并制备Lac cDAN探针,为进一步研究乳糖不耐受奠定基础。方法 取4周龄BALB／c小鼠小肠组织,用Trizol试剂盒提取总RNA,经RT—PCR、梯度PCR获取Lac cDNA。测序鉴定后将序列提交NCBI GenBank,同时利用生物信息学,对其编码产物进行预测。探针的制备采用随机引物法以地高辛标记。结果 所获得的Lac cDNA编码区有912bp,编码303个氨基酸残基,在其二级结构中存在一个糖苷键水解酶基序,C-末端有一疏水螺旋区。标记后的探针经检测,灵敏度达到1pg／μl。结论 所获得的Lac基因经鉴定确为乳糖酶基因,由它编码的蛋白产物是乳糖酶。标记的探针灵敏度很高,可以用于原位杂交实验。     

Friend鼠白血病病毒对BALB/c小鼠和KM小鼠的影响

目的：探讨Friend鼠白血病病毒（FLV）对BALB/c和KM小鼠体质量、胸腺和脾脏的影响。方法：以齐多夫定片（AZT）作为阳性药物,用FLV攻击BALB/c和KM小鼠,观察各鼠系的体质量、胸腺和脾脏变化情况。结果：FLV能引起BALB/c和KM小鼠脾肿大,各鼠系脾指数与正常鼠脾指数差异有统计学意义（P〈0.01）。AZT对BALB/c和KM小鼠具有明显的抑制脾肿大作用（P〈0.05）。各鼠系小鼠的体质量和胸腺变化差异无统计学意义（P〉0.05）。结论：FLV对KM小鼠体质量、胸腺和脾脏的影响程度相当于BALB/c小鼠。     

Immunotoxicological Evaluation of Wheat Genetically Modified with TaDREB4 Gene on BALB/c Mice

ObjectiveTo evaluate the immunotoxicological effects of genetically modified wheat with TaDREB4 gene in female BALB/c mice.MethodsFemale mice weighing 18-22 g were divided into five groups (10 mice/group), which were set as negative control group, common wheat group, parental wheat group, genetically modified wheat group and cyclophosphamide positive control group, respectively. Mice in negative control group and positive control group were fed with AIN93G diet, mice in common wheat group, non-genetically modified parental wheat group and genetically modified wheat group were fed with feedstuffs added corresponding wheat (the proportion is 76%) for 30 days, then body weight, absolute and relative weight of spleen and thymus, white blood cell count, histological examination of immune organ, peripheral blood lymphocytes phenotyping, serum cytokine, serum immunoglobulin, antibody plaque-forming cell, serum half hemolysis value, mitogen-induced splenocyte proliferation, delayed-type hypersensitivity reaction and phagocytic activities of phagocytes were detected.ResultsNo immunotoxicological effects related to the consumption of the genetically modified wheat were observed in BALB/c mice when compared with parental wheat group, common wheat group and negative control group.ConclusionFrom the immunotoxicological point of view, results from this study demonstrate that genetically modified wheat with TaDREB4 gene is as safe as the parental wheat.     

Acute and Sub-chronic Toxicity of Tetrandrine in Intravenously Exposed Female BALB/c Mice

Objective: To evaluate the acute and sub-chronic toxicity of intravenously administered tetrandrine(TET) in female BALB/c mice. Methods: The median lethal dose(LD_(50)) of intravenously administered TET was calculated in mice using Dixon's up-and-down method. In the acute toxicity study, mice were intravenously administered with TET at a single dose of 20, 100, 180, 260 and 340 mg/kg, respectively and were evaluated at 14 days after administration. In the sub-acute toxicity study, mice were intravenously administered various doses of TET(30, 90 and 150 mg/kg) each day for 14 consecutive days. Clinical symptoms, mortality, body weight, serum biochemistry, organ weight and histopathology were examined at the end of the experiment, as well as after a 1-week recovery period. Result: LD_(50) was found to be 444.67±35.76 mg/kg. In the acute toxicity study, no statistically significant differences in body weight, blood biochemistry, or organ histology were observed between the administration and control groups when mice were intravenously administered with single dose at 20, 100, 180, 260 and 340 mg/kg of TET(P0.05). In the sub-acute toxicity study, no significant changes in body weight, biochemistry and organ histology were observed with up to 90 mg/kg of TET compared with the control group(P0.05), however, in the 150 mg/kg administered group, TET induced transient toxicity to liver, lungs and kidneys, but withdrawal of TET can lead to reversal of the pathological conditions. Conclusions: The overall findings of this study indicate that TET is relatively non-toxic from a single dose of 20, 100, 180, 260 or 340 mg/kg, and that up to 90 mg/kg daily for 14 consecutive days can be considered a safe application dose.     

用核酸杂交检测心肌炎小鼠心肌中柯萨奇B病毒RNA

作者报道了柯萨奇B_(3m)病毒感染BALB/c小鼠后,用常规病毒分离方法,于感染后第9天心肌内病毒分离开始转阴,而使用cDNA-RNA分子杂交方法,在感染后观察的整个36天内,小鼠心肌内病毒基因一直存在,提示病毒性心肌炎病变的持续存在可能与病毒在心肌中的继续作用有关。     

登革病毒不同途径感染Balb/C小鼠的比较

目的:比较Balb/C小鼠不同途径接种登革病毒（DEN）产生的临床和病理学变化。方法：分别以颅内注射、腹腔注射、皮下多点注射、混合注射等方式将登革2型病毒GZ14株接种到Balb/C小鼠体内,观察小鼠临床表现、体征、血清及内脏中病毒感染情况。结果：各组小鼠均未出现典型的临床症状,但可以从体内分离出登革病毒。当感染方式不同时,血液检测结果及脏器损伤的情况也存在着一定的差异。结论：不同的接种方式均可以使小鼠感染登革病毒,其中以混合注射的方式效果最佳。