南平市城乡中小学生肠道线虫感染情况调查

本文对城乡中、小学生929人进行粪检,感染肠道线虫440人,感染率为47.36％,其中蛔虫、鞭虫、晓虫、钩虫感染率分别为41.44％、11.30％、1.61％和0.54％;单纯感染率为38.97％,混合感染率为8.40％;城镇学生感染率为41.79％,农村学生感染率为58.28％;小学生感染率为40.82％,中学生感染率为52.62％;城乡学生之间、中小学生之间肠道线虫感染有非常显著差异。     

2810例老年人尿液常规检测结果的临床分析

目的：对老年人尿液常规检测结果进行分析,了解老年患者健康状况。方法：抽选2016年1月－2016年8月间2810例参加健康体检的老年人群,所有人员均进行尿检,对其尿检结果进行分析并统计相关数据。结果：2810例患者白细胞检出率为12．7％、亚硝酸盐检出率为3．24％、尿蛋白检出率为5．44％、隐血检出率为31．92％、酮体检出率为2．38％、尿糖检出率为2．74％。女性人群白细胞检出率为19．77％、亚硝酸盐检出率5．13％尿蛋白检出率为4．82％、隐血检出率为39．10％、酮体检出率为1．79％、尿糖检出率为2．16％。男性人群白细胞检出率为3．11％、亚硝酸盐检出率0．67％尿蛋白检出率为6．30％、隐血检出率为22．17％、酮体检出率为3．19％、尿糖检出率为3．53％。结论：老年人群尿检阳性结果较高,且女性高于男性,因此要对对泌尿系统早期症状进行治疗,预防疾病发生。     

农村急性心肌梗死临床分析

探讨农村急性心肌梗死的发病情况、临床特点及急性期预后。发病五大危险因素中高血压和吸烟分别为37.11％和31.29％,糖尿病、高血脂、肥胖各占13.31％、12.23％、5.04％。急性期的主要并发症,各种心律失常为58.27％,心力衰竭为15.11％,心源性休克为11.51％,其它为4.68％,与对照资料比较无统计学意义。     

74例霉菌性阴道炎病原菌分析及酮康唑治疗

本文对74例霉菌性阴道炎患者的阴道分泌物进行了真菌检查,直接镜检阳性率为94.59％,真菌培养阳性率为86.49％,白念球菌占培养阳性的72.72％;伪热带念珠菌占1.52％;类星形念珠菌占3.03％;酵母菌占18.18％,酵母样菌占4.55％。酮康唑治疗其中41例,痊愈率为78.05％,总有效率为100％,不良反应发生率为31.72％。较多的表现为头晕、恶心、皮肤瘙痒,少数表现为头痛、腹痛、腹泻、皮肤起风团,肝功能异常。     

淄博市医疗单位消毒工作现状调查分析

淄博市56所各级医院室内空气消毒合格率为46.30％～57.45％,物体表面消毒合格率为59.03％～90.10％,医护人员手的消毒合格率为70.49～85.53％,压力蒸气灭菌、使用中消毒液与紫外线灯辐照度值合格率分别为79.31％～100％、75％～95.24％、60.42％～97.90％。     

不同民族肠道寄生虫感染情况的调查研究

本文对5种民族1644人粪检,寄生虫感染1171人,感染率高达71.22％。检出肠道寄生虫4种,即蛔、鞭、钩和蛲虫,感染率分别为56.56％、30.47％、4.98％和4.56％;单纯感染率为47.93％,混合感染率为23.29％;5种民族汉、彝、傣、回和蒙古族感染率依次为58.84％、79.88％、52.63％、70.44％和89.78％。蛲虫感染在傣族中具有家庭聚集性。     

胶囊内镜在消化系统疾病中的诊断价值

目的：探讨胶囊内镜在消化系统（尤其是小肠）疾病中的诊断价值。 方法: 对北京大学第三医院100例M2A胶囊内镜检查患者进行临床资料分析和电话随访。 结果: 在不明原因消化道出血、腹痛或腹部不适和腹泻患者中,胶囊内镜检查异常病变发现率分别为67.6％、42.9％和44.4％,临床确诊率为60.0％、80.0％和72.2％,胶囊内镜检查准确率为75.0％、92.9％和84.6％;对小肠病变的诊断敏感性为77.3％、87.5％和66.7％,特异性为50.0％、95.0％和90.0％,阳性预测值为94.4％、87.5％和66.7％,阴性预测值为16.7％、95.0％和90.0％。使用甲氧氯普胺（10 mg肌注）组患者的胶囊内镜胃通过时间明显短于未使用组（15.0 min vs 30.5 min,P=0.019）。 结论: 胶囊内镜在诊断小肠疾病方面是一种无创、安全且有效的检查方法,甲氧氯普胺有助于缩短胶囊内镜的胃通过时间。     

不稳定型心绞痛早期诊断指标的临床研究

目的研究早期诊断不稳定型心绞痛的高敏感性和高特异性的血清标记物。方法将200例诊断为不稳定型心绞痛患者分成两组,分别对他们采取血液进行肌钙蛋白T（TNT）、CK、CK—MB、Mb、MLC-1（肌球蛋白轻链-1）水平的定量检测,分析其阳性率、敏感性和特异性。结果阳性率：TNT为92．9％,CK为25．4％,CK—MB为34．3％,Mb为23．1％,MLC-1为18.3％;敏感性：TNT为100．0％,CK为35．2％,CK—MB为50．1％,Mb为26．3％,MLC-1为17．2％;特异性：TNT为92．9％,CK为25．4％,CK—MB为34．3％,Mb为23．1％,MLC-1为18．3％。结论TNT阳性结果对不稳定型心绞痛患者发生心肌梗死具有高度的预见性,因此TNT可以作为一种排除不稳定型心绞痛患者发生心肌梗死的标记物。     

常规凝血实验与血栓弹力图评价凝血异常患者的临床价值

目的:分析常规凝血实验与血栓弹力图评价凝血异常患者的临床价值.方法:选择东莞市人民医院2019年2月—2020年7月收治的重症监护室凝血异常的患者50例.比较常规凝血实验与血栓弹力图试验结果的Kappa一致性和准确度.结果:R与APTT、PT判断是否低凝的一致性的Kappa值分别为0.246、0.383(均P<0.01).Fib与K、α-Angle、MA判断是否低凝的一致性的Kappa值为分别为0.458、0.330、0.545(均P<0.01).PLT与K、α-Angle、MA判断是否低凝的一致性的Kappa值分别为0.453、0.492、0.580(均P<0.01).R判断临床输血的ROC曲线下面积AUC为0.672,敏感性为58％,特异性为82％;K的AUC为0.815,敏感性为80％,特异性为74％;α-Angle的AUC为0.839,敏感性为84％,特异性为78％;MA值的AUC为0.802,敏感性为70％,特异性为86％;PT的AUC为0.822,敏感性为58％,特异性为94％;APTT的AUC为0.870,敏感性为74％,特异性为90％;Fib的AUC为0.766,敏感性为96％,特异性为52％;PLT的AUC为0.872,敏感性为78％,特异性为94％.结论:常规凝血实验与血栓弹力图在评价患者凝血异常上有价值,综合二者检测结果能更准确判断患者凝血功能.     

HPLC Simultaneous Determination of Six Lignans in Schisandra vinegar Products from Different Habitats

目的 测定不同产地醋五味子中五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素6种木脂素的含量.方法 色谱柱为依利特ODS-C18 (250 mm×4.6 mm,5μm);流动相为乙腈(A)—水(B),梯度洗脱(0～17 min,A为50％;17～25 min,A为50％～55％ ;25～30 min,A为55％～75％;30～35 min,A为75％;35～40min,A为75％～65％ ;40～45 min,A为65％～50％).结果 6种被测木脂素成分分离度良好;各成分质量浓度与峰面积在测定范围内均呈良好的线性关系(r＞0.999 5);重现性良好;准确度(RSD)分别为101.8％(1.5％)、99.5％(1.2％)、103.26％ (2.1％)、99.8％(1.0％)、100.0％(1.9％)和101.3％(1.9％).结论 HPLC法同时检测五味子6种木脂素成分,所建立方法稳定、可行.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.