中医药治疗白内障术后并发大泡性角膜病变3例

大泡性角膜病变是白内障手术后的严重并发症之一,目前尚无特效药物和治疗方法.我院1997年6月～2005年6月施行白内障超声乳化术4973例中医药发生大泡性角膜病变3例(3只眼),经中医药治疗后两例取得满意效果.报告如下.     

大泡性角膜病变的手术治疗

目的 探讨用手术方法治疗大泡性角膜病变的实用价值.方法 对30例(30眼)确诊为大泡性角膜病变的患者,行小切口角膜层间植入羊膜25例.亚全板层角膜移植术5例.结果 术后随访5个月～5年,平均2年.两种术式治疗的病例症状均消失,角膜上皮完整光滑.小切口角膜层间植入羊膜病例,两周内羊膜组织均匀平复,呈灰白色水肿,四周后羊膜变为淡灰色.1年后羊膜植入物呈薄纱样改变.5例亚全板层角膜移植病例,视力均有不同程度提高,分别由眼前手动、数指提高到0.04～0.2.角膜半透明愈合3例,透明愈合2例.结论 小切口角膜层间植入羊膜,手术简单,创伤小,亚全板层移植排斥机会少,可改观外观,提高视力.     

白内障超声乳化术后大泡性角膜病变临床分析

1967年Kelman应用超声乳化仪后白内障手术发生了一个划时代的变化。手术以切口小、散光少、恢复快 ,不需住院 ,在临床上广泛开展。我院自 1996年12月开展白内障超声乳化手术 ,病人术后视力恢复快 ,无暴发性脉络膜出血、球后出血等。但手术的开展 ,尤其是早期 ,也出现了一些并发症 ,如角膜失代偿 ,总结如下。1　资料和方法白内障超声乳化手术患者 492例 ,年龄 5～ 87岁 ,发生角膜失代偿者 8例 9只眼 ,其中女性 6例 ,男性2例。此 8例年龄 3 2岁～ 78岁。老年性白内障 7例 ,角膜穿孔伤合并外伤性白内障 1例。老年性白内障核为Ⅱ～Ⅳ级。使用眼…     

大泡性角膜病变行角膜层间分离清刮术治疗临床观察

目的：研究角膜层间分离清刮术治疗大泡性角膜病变的临床效果。方法：对8例大泡性角膜病变患者行7～8mm深达1／2角膜的层间分离及清刮术。结果：角膜愈合良好,无眼痛、异物感症状消失,视力改善,随访2年,治疗效果稳定。结论：角膜层问分离清刮术是一种简便、安全、较为理想的手术方法,为不具备穿透性角膜移植条件患者理想选择。     

新鲜羊膜移植治疗大泡性角膜病变2例

大泡性角膜病变是白内障摘除术后较为严重的并发症之一 ,患者眼痛剧烈 ,眼磨、畏光、流泪 ,影响生活。笔者在临床遇到 2例 ,经给予新鲜羊膜移植取得了良好效果 ,现报道如下。1　病历介绍例 1:男 ,5 3岁 ,主因右眼缓慢视力下降 2a,眼痛、眼红伴头痛、恶心 4d ,于 2 0 0 2年 2月     

中药治疗浅层点状角膜病变临床观察

浅层点状角膜病变是一种病程缓慢，迁延难愈的常见眼病。现将作者自１９９２年８月至１９９７年４月以中药为主的方法治疗本病的结果报告如下。１资料与方法１．１临床资料本组治疗５３例９６只眼，其中双眼４３例８６只眼，单眼１０例１０只眼。男３５例，女１８例。年龄...     

清障明目汤治疗内眼术后大泡性角膜病变9例

随着各种眼前段手术的开展与普及 ,内眼术后发生大泡性角膜病变的病例逐渐增多。临床上多见病人剧烈眼痛 ,容易致盲。我科从1 998年 2月至 2 0 0 1年 1 0月用单纯中药治疗 9例 9只眼内眼术后大泡性角膜病变 ,收到良好效果 ,小结如下。1　一般资料病例总数 9例 9只眼 ,均为我科门诊治疗观察的病例。其中男性 2例 ,女性 7例 ;年龄最小 55岁 ,最大 80岁 ;发病时间最短为术后半月 ,最长为术后 3 2年。引起角膜大泡疼痛的原因均为老年性白内障术后并发大泡性角膜病变。眼别为右眼 4例 ,左眼 5例 ;就诊时视力最差为眼前手动 ,最好为 0 .1。全部病…     

针刺治疗大泡性角膜炎的临床观察

大泡性角膜病变,是由于角膜上皮及实质层长期持续水肿,致使角膜上皮和上皮下形成大小不等的水泡为特征的角膜变性疾病。弥漫性上皮水肿多因眼内压陡然升高至50mmHg以上(正常眼压为10～21mmHg),基质水肿的原因为内皮屏障或原功能不全,致基质层增厚,严重者可波及上皮,引起大泡性角膜上皮病变。自1984年3月～1995年12月共治疗本病28例28只眼。报道如下。1　临床资料28例中男19例,女9例;年龄最小29岁,最大76岁。于手术后并发的19例(术后6个月～5年),外伤后引起7例(外伤后5个月～6年),角膜炎症继发的2例。2　治疗方法取穴:眼部主穴为承泣、睛明…     

角膜宁汤治疗特殊性角膜病变3例

角膜宁汤治疗特殊性角膜病变３例王克长角膜宁汤组方：玄参、苦参、连翘、桔梗、柴胡、板蓝根、黄芩、黄连、黄柏、金银花、菊花、薄荷、生地、麦冬。用药方法：用水煎剂，一煎后分３次口服；二煎多加水，待煮沸后以蒸气熏敷患眼１５～２０分钟，１日３次，每日１剂。例１...     

中药治疗糖尿病性视网膜病变临床观察

中药治疗糖尿病性视网膜病变临床观察李荣珍，李炜倩糖尿病性视网膜病变是糖尿病的眼部并发症，是眼科常见的致盲眼病之一。约占糖尿病患者的４５％～５８％［１］。在治疗方面目前一般认为应以激光光凝为治疗的主要手段，药物治疗的效果尚难肯定［２］，因此探讨在基层医...     

Effects of acacia honey on wound healing in various rat models

Honey is a traditional remedy for the treatment of infected wounds, and is becoming more important as microbial resistance to conventional therapeutic agents increases. A study was conducted to assess the wound‐healing activity of Acacia honey using incision, excision, burn and dead‐space wound models in rats. Different formulations of honey were used and rats were treated topically as well as orally. Both the higher and lower doses of honey produced a significant effect on healing (p < 0.05). The area of epithelization was found to increase, followed by an increase in wound contraction, skin‐breaking strength, tissue granulation. The hydroxyproline content also increased in the rats treated with higher doses of honey compared to control, indicating an increase in collagen formation. Copyright © 2009 John Wiley & Sons, Ltd.     

Antinociceptive activity of natural honey in thermal-nociception models in mice

Several honey samples of Pakistani origin have been analysed for their effect on nociception. Among the tested samples, Acacia honey showed most effective dose-dependent antinociceptive activity which was significantly different from the untreated group in tail-flick and paw-withdrawal tests (p < 0.01 and p < 0.003), respectively. The antinociceptive activity of honey reached a maximum at 60 min post-treatment and then gradually decreased, whereas the activity of indomethacin was persistent at 120 min post-treatment. The absence of antinociceptive activity in simulated honey (which contained fructose, glucose, maltose and sucrose representing the major constituents of honey) indicated that the active principle(s) might be present in minor constituents of honey.     

Anti inflammatory effect of natural honey on bovine thrombin‐induced oxidative burst in phagocytes

Thrombin, hyperglycemia and reactive oxygen species (ROS) have been discovered to play a pivotal role in the pathogenesis of cardiovascular disease (CVD). The aim of the study was to evaluate the direct effect of bovine thrombin (BTh) on ROS production by human neutrophils and rodent macrophages and to investigate the effect of honey on BTh‐induced ROS production from phagocytes. Professional phagocytes, i.e. neutrophils and macrophages, were stimulated by BTh and ROS production was measured in luminol/lucigenin enhanced chemiluminescence (CL) assays. In another experiment the effects of honey treatment on BTh‐induced ROS production by phagocytes was tested using a CL assay. The results indicate that BTh directly activates phagocytes. A significant generation of ROS was noted with the luminol/lucigenin enhanced chemiluminescence (CL) system. Honey treatment of phagocytes activated by bovine thrombin showed effective suppression of oxidative respiratory burst monitored by the CL assay. In conclusion, it can be assumed that this direct action of BTh on phagocytes causing ROS production might exaggerate the inflammatory response at the site of atheromatous plaques. The suppressive activity of honey towards thrombin‐induced ROS production by phagocytes could be beneficial in the interruption of the pathological progress of CVD and may play a cardioprotective role. Copyright © 2009 John Wiley & Sons, Ltd.     

补肾中药对雄激素致不孕大鼠胰腺及卵巢雄激素受体的影响

目的:探讨补肾中药天癸方对雄激素致不孕大鼠(ASR)促排卵的作用机制。方法:新生SD雌性大鼠随机分为3组,ASR模型组:9日龄SD雌性大鼠皮下注射丙酸睾丸酮;中药组:ASR模型在80日龄起每日灌服天癸方;对照组:9日龄SD雌性大鼠皮下注射中性茶油。在106日龄左右(动情前期),放免法测定血Δ4-雄烯二酮(Δ4-A)、总睾酮(TT)、游离睾酮(FT)、雌二醇(E2)、胰岛素(Ins)和C-肽(C-P),RT-PCR方法检测胰腺及卵巢雄激素受体(AR)mRNA表达水平,并观察中药治疗前后上述指标的变化。结果:ASR模型血Δ4-A、TT、FT、E2、Ins、C-P均显著高于对照组(P<0.05,P<0.01),胰腺、卵巢的AR mRNA表达水平明显升高(P<0.01),中药天癸方治疗后,排卵率为58%,各项血激素及胰腺AR mRNA表达水平明显下降,与对照组比较有显著性差异(P<0.05),卵巢AR mRNA水平与治疗前相比无明显改变。结论:ASR模型血雄激素水平升高,上调胰腺、卵巢的AR mRNA表达,引起高胰岛素血症和无排卵。天癸方可降低血雄激素和胰岛素,使胰腺AR mRNA表达水平下降,纠正ASR模型的内分泌-代谢失调状态,虽然卵巢的AR mRNA表达水平仍较高,但在正常的激素环境下仍出现了排卵。     

A comparison of wound healing following treatment with Lavandula x allardii honey or essential oil

The increased interest in complementary therapies has led to the investigation of products traditionally believed to have a beneficial effect in wound healing. Two such products are honey and lavender essential oil. In this study a rat excisional wound model was used to investigate the action of Lavandula x allardii honey and essential oil, and a standard therapeutic honey (Medihoney). Four 8 mm wounds were created surgically on the dorsal surface of each rat and honey or essential oil applied to the wounds twice a day for 4 days. Wound healing was analysed by wound contraction and capillary volume at 5 and 12 days post-surgery. Although no statistically significant difference in wound contraction was observed for the essential oil or honey treated wounds relative to the untreated control, both honeys were shown to reduce the capillary volume in the wound site at day 12 with no difference between the honeys (control 154 +/- 14 microm(3) vs L. x allardii honey 77 +/- 18 microm(3) and Medihoney 89 +/- 39 microm(3), p < 0.001; mean +/- SD). This suggests that scar maturation in these animals was more advanced than in other groups. These data suggest that L. x allardii honey, but not essential oil, has a beneficial action in wound healing.     

蜂蜜、蜂胶对大鼠皮肤溃疡创面愈合的影响

[目的]研究蜂蜜、蜂胶外用对皮肤溃疡模型大鼠创面愈合的影响。[方法]采用2.5 mol/L NaOH外涂建立大鼠皮肤溃疡模型。观察蜂蜜、蜂胶外用对溃疡创面愈合时间的影响,观察创面皮肤组织学变化,检测大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。[结果]蜂蜜、蜂胶外用能明显缩短创面愈合时间(P<0.05或P<0.01);能显著提高大鼠血清中SOD含量(P<0.05或P<0.01);能降低血清中MDA含量(P>0.05)。[结论]蜂蜜、蜂胶外用能够促进皮肤溃疡创面愈合。     

补肾中药天癸方对雄激素致不孕大鼠胰腺及卵巢雄激素受体的影响

目的:探讨补肾中药天癸方对雄激素致不孕大鼠(ASR)促排卵的作用机制。方法:新生SD雌性大鼠随机分为3组,ASR模型组:9d龄SD雌性大鼠皮下注射丙酸睾丸酮;中药组:ASR模型在81d龄起每日灌服天癸方;对照组:9d龄SD雌性大鼠皮下注射中性茶油。在106d龄左右(动情前期),放免法测定血Δ4-雄烯二酮(Δ4-A)、总睾酮(TT)、游离睾酮(FT)、雌二醇(E2)、胰岛素(Ins)和C-肽(C-P),RT-PCR方法检测胰腺及卵巢雄激素受体(AR)mRNA表达水平,并观察中药治疗前后上述指标的变化。结果:ASR模型血Δ4-A、TT、FT、E2、Ins、C-P均显著高于对照组(P<0.05,P<0.01),胰腺、卵巢的AR mRNA表达水平明显升高(P<0.01);中药天癸方治疗后,排卵率为58%,各项血激素及胰腺AR mRNA表达水平明显下降,与模型组比较有显著性差异(P<0.05,P<0.01),卵巢AR mRNA水平与治疗前相比无明显改变。结论:ASR模型血雄激素水平升高,上调胰腺、卵巢的AR mRNA表达,引起高胰岛素血症和无排卵。天癸方可降低血雄激素和胰岛素,使胰腺AR mRNA表达水平下降,纠正ASR模型的内分泌-代谢失调状态,虽然卵巢的AR mRNA表达水平仍较高,但在正常的激素环境下仍出现了排卵。     

金匮肾气丸与糖皮质激素配伍治疗大疱性类天疱疮临床疗效观察

目的 观察金匮肾气丸联合糖皮质激素治疗大疱性类天疱疮的临床疗效、激素用量,探讨中西医结合方法治疗类天疱疮的可行性、安全性.方法 选择经临床及病理诊断的大疱性类天疱疮30例,随机分为2组,对照组给予泼尼松0.75 mg/(kg·d),研究组在此基础上加服金匮肾气丸1丸/次,2次/d.采用《大疱病临床研究观察表》记录患者每天新发水疱数、全身总水疱数和激素剂量等.结果 两组患者每天新发水疱数、全身总水疱数均减少,研究组较对照组水疱数减少更为明显(P＜0.01),但激素剂量和不良反应均小于对照组(P＜0.01).结论 金匮肾气丸与糖皮质激素配伍治疗类天疱疮具有疗效高、安全性高、不良反应小的特点,是值得临床推广的中西医结合治疗手段.     

紫云英蜜中总酚的含量测定

目的：建立紫云英蜜中总酚含量测定方法。方法：采用Folin酚法,样品经福林试剂显色反应后,紫外可见分光光度计测定其总酚含量。结果：总酚在2.03～8.04μg·mL^-1内线性良好,r=0.9997,且平均回收率为102H42%,RSD=2.64%（n=5）。结论：该方法准确,简便,重现性好,专属性强,可有效地控制紫云英蜜的质量。     

Effect of honey on intestinal morphology, intraabdominal adhesions and anastomotic healing

In the present study, attempts have been made to determine the effects of honey on intestinal morphology, postoperative adhesions, and the healing of colonic anastomoses in the rats after colonic resection and anastomosis. Thirty-six rats were randomly divided into three groups each including 12 animals. Colonic resection and anastomosis were performed on all animals. Rats were fed with standard rat chow in group I, standard rat chow plus 10 g/kg/day honey in group II and artificial honey including the same caloric amount with honey in group III. Adhesion scores, bursting pressures and histopathological examinations were evaluated. Colonic bursting pressures of honey group were significantly better than control and artificial honey groups. Histological analysis of anastomotic site showed that submucosa and muscularis propria were nearly filled with granulation tissue and regular fibrin matrix in honey group. There was statistically significant difference between the adhesion scores of honey vs artificial honey and control groups. The scores of histological changes of ileum in honey group were significantly different from other groups. These results indicate a protective role of honey against intraabdominal adhesions and anastomotic dehiscence.