抗皮肤癣菌用姜油树脂凝胶的质量控制

[目的]建立抗真菌用姜油树脂凝胶制剂的质量控制标准。[方法]以6-姜酚为对照品,采用高效液相色谱法,色谱柱为HYPERSIL BDS C18(5μm,4.6 mm×250 mm)加预柱,流动相为甲醇∶水=65∶35,检测波长280 nm,测定姜油树脂凝胶中6-姜酚的含量。[结果]6-姜酚的线性范围是0.06~1.2 mg/mL,r=0.9999;精密度、重复性、稳定性的RSD值均<2%,平均回收率为98.30%,RSD=0.95%。[结论]本实验建立的测定姜油树脂凝胶中6-姜酚含量的高效液相色谱法,结果可靠,操作简便,可以作为姜油树脂凝胶的质量控制标准。     

HPLC法测定复方桔梗止咳片中甘草酸的含量

[目的]建立复方桔梗止咳片中甘草酸的含量测定方法。[方法]用高效液相色谱法测定。色谱柱为Kromasil C18柱(4.6mm×250mm,5μm);流动相为甲醇-水-冰醋酸-三乙胺(58∶42∶1∶0.3);流速0.8ml/min;检测波长250nm;柱温为室温。[结果]甘草酸单铵盐在进样量0.0857~1.0284μg范围内线性关系良好,r=0.9999,方法回收率为98.42%,RSD=0.78%(n=6)。[结论]结果表明HPLC法测定该制剂中甘草酸的含量专属性强,重现性好,方法可行。     

HPLC法测定鼻窦炎合剂中盐酸麻黄碱的含量

[目的]建立HPLC法测定鼻窦炎合剂中盐酸麻黄碱的含量。[方法]用Hypersil ODS2色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.01 mol/L磷酸二氢钾缓冲液(用磷酸调至pH 2.5)(15∶85)为流动相,柱温为30℃,流速为1.0 ml/min,检测波长210 nm。[结果]盐酸麻黄碱进样量在2～10μg内线性关系良好(r=0.9995,n=5),平均加样回收率为97.9%,RSD=1.46%(n=6)。[结论]HPLC法操作简便,结果准确,可用于鼻窦炎合剂的质量控制。     

新型二维高效液相色谱测定人血清伏立康唑浓度方法的建立与应用

目的建立全自动二维高效色谱法(2D-HPLC)测定血清中伏立康唑的浓度并应用于临床。方法第一维色谱柱为ASTON SC2柱(4.6mm×25mm,5μm),流动相为20mmol/L磷酸铵∶乙腈∶甲醇=3∶1∶1(V∶V∶V,磷酸调pH=5.4),流速1.2mL/min;第二维色谱柱为SHIMADZU C18柱(4.6 mm×150 mm,5μm),流动相为10mmol/L醋酸铵(三乙胺调pH7.0)∶10mmol/L醋酸铵(三氟乙酸调pH3.0)∶甲醇∶乙腈=30∶10∶10∶50(V∶V∶V∶V),流速1.0mL/min;中间柱为ASTON SH柱(3mm×10mm,5μm),辅助流动相为纯水。样品由一维色谱柱萃取,经中间柱捕获并转移至第二维色谱柱分析,检测波长256nm,柱温40℃,进样量200μL。结果伏立康唑在0.28～22.56μg/mL范围内线性关系良好,r=0.999 9,最低定量限为0.28μg/mL,方法回收率为99.01%～104.62%,提取回收率为85.81%～89.82%,日内、日间精密度RSD均小于9%。结论该方法操作简单、准确、灵敏,符合血清样品的测定要求,适用于伏立康唑临床血药浓度测定。     

HPLC法测定风热感冒颗粒中牛蒡苷的含量

[目的]建立风热感冒颗粒的含量测定方法。[方法]采用HPLC法,色谱柱:Phenomenex of Synergi C18柱(250mm×4.6 mm,5μm);流动相:甲醇-水(36∶64);检测波长:280nm。[结果]牛蒡苷在0.04μg~0.2μg范围内呈良好的线性关系,平均加样回收率为98.76%,RSD=1.37%(n=6)。[结论]HPLC法专属性强,定量方法简便、准确,能有效地控制制剂的质量。     

HPLC法测定三味清热止痒洗剂中麻黄碱的含量

[目的]建立HPLC法测定三味清热止痒洗剂中麻黄碱含量的方法。[方法]用Hypersil ODS2色谱柱(250mm×4.6mm,5μm),以甲醇-0.01mol/L磷酸二氢钾缓冲液(用磷酸调至pH 2.5)(15∶85)为流动相,流速为1.0ml/min,检测波长210nm。[结果]麻黄碱进样量在4μg~12μg内线性关系良好(r=0.9996,n=5),平均加样回收率为97.35%,RSD=0.98%(n=6)。[结论]HPLC法操作简便,结果准确,可用于三味清热止痒洗剂的质量控制。     

HPLC测定罗汉果中罗汉果甜苷V的含量

[目的]建立HPLC法测定罗汉果药材中罗汉果甜苷V的含量。[方法]用Nucleosil 100-5 NH2(5μm,4.6mm×250mm)色谱柱,以乙腈-水-2.5%四氢呋喃(77∶22∶1)为流动相,流速为1ml/min,检测波长为203nm,柱温30℃。[结果]罗汉果甜苷V进样量在0.2136~21.3600μg线性关系良好(r=0.99993),平均回收率为96.96%,RSD为0.72%(n=5)。[结论]本方法简便、快速、准确,可作为罗汉果药材的质量控制方法。     

不同产地八角中莽草酸含量比较研究

[目的]建立高效液相色谱法测定八角中莽草酸含量,对比不同产地八角中莽草酸含量。[方法]HPLC条件:色谱柱为5NH2-MS(4.6μm×250 mm),流动相:乙腈-水(0.1%H3PO4)(15∶85),流速1.0 ml/min,检测波长213 nm;用不同比例的甲醇水超声提取莽草酸,对比其含量。[结果]莽草酸进样量在3.03~15.05μg范围内与峰面积线性关系良好。回归方程Y=13 144.16X-0.68,(n=6,r=0.999 99)。平均回收率为100.09%,RSD=0.63%;提取率:甲醇<70%(甲醇-水)<50%(甲醇-水)<30%(甲醇-水)<水,三种八角中含量八角(2)>八角(1)>八角(3)。[结论]建立的HPLC方法适合测定八角中莽草酸的含量;甲醇-水混合溶剂从八角中提取莽草酸,水是最好的提取溶剂,八角(2)中莽草酸含量最高。     

RP-HPLC测定金不换中延胡索乙素的含量

[目的]建立HPLC法测定金不换中延胡索乙素的含量。[方法]以Shim-pack CLC-ODS C18柱(150mm×5mm,5μm)为色谱柱,甲醇∶水(70∶30)为流动相,流速1.0ml/min,检测波长为280nm,用外标法定量。[结果]延胡索乙素在0.487~2.922μg范围内有良好的线性关系,r=0.9998,平均回收率为92.53%,RSD为2.38%(n=5)。[结论]本方法测定金不换中延胡索乙素含量可靠、简便。     

荷叶总黄酮苷质量控制研究

[目的]建立荷叶提取物中黄酮类化合物的高效液相色谱测定方法。[方法]药材经提取水解后用HPLC通过测定其槲皮素的含量来确定荷叶提取物中总黄酮的含量,采用Shim-packCLC-ODS(4.6mm×150mm,5μm)色谱柱,甲醇-水-磷酸(50∶50∶0.1)为流动相,流速0.8mL/min,检测波长360nm。[结果]槲皮素在0.013～0.130μg范围内呈良好线性关系(r=0.9999),荷叶提取物中槲皮素的平均含量为4.94%,RSD=1.67%;平均回收率为99.15%,RSD=0.60%。[结论]此测定方法简便快捷、精密度高、准确性好,可以作为荷叶中总黄酮的含量测定方法,以控制荷叶黄酮苷的质量。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.