Evaluation of the redox state in mouse organs following radon inhalation

Radon inhalation activates antioxidative functions in mouse organs, thereby contributing to inhibition of oxidative stress-induced damage. However, the specific redox state of each organ after radon inhalation has not been reported. Therefore, in this study, we evaluated the redox state of various organs in mice following radon inhalation at concentrations of 2 or 20 kBq/m³ for 1, 3 or 10 days. Scatter plots were used to evaluate the relationship between antioxidative function and oxidative stress by principal component analysis (PCA) of data from control mice subjected to sham inhalation. The results of principal component (PC) 1 showed that the liver and kidney had high antioxidant capacity; the results of PC2 showed that the brain, pancreas and stomach had low antioxidant capacities and low lipid peroxide (LPO) content, whereas the lungs, heart, small intestine and large intestine had high LPO content but low antioxidant capacities. Furthermore, using the PCA of each obtained cluster, we observed altered correlation coefficients related to glutathione, hydrogen peroxide and LPO for all groups following radon inhalation. Correlation coefficients related to superoxide dismutase in organs with a low antioxidant capacity were also changed. These findings suggested that radon inhalation could alter the redox state in organs; however, its characteristics were dependent on the total antioxidant capacity of the organs as well as the radon concentration and inhalation time. The insights obtained from this study could be useful for developing therapeutic strategies targeting individual organs.     

二氧化硫对小鼠超氧化物歧化酶活性的影响

[目的]观察二氧化硫（SO2）对小鼠9种脏器超氧化物歧化酶（SOD）活性的影响。[方法]采用整体动物吸入染毒方式，测定小鼠吸入SO2气体后不同脏器SOD活性的变化。[结果]小鼠吸入较低浓度的SO2后，SOD活性变化因脏器而异；肝，肾，心，脾，脑，小肠，睾丸组织的SOD活性呈下降趋势，肺和胃组织的SOD活性呈上升趋势，但在较高SO2吸入浓度下，9种脏器SOD活性显著下降。[结论]SO2吸入体内会引起机体上述组织抗氧化能力下降。造成机体的氧化损伤。     

Indoor radon in Kuwait

Indoor radon measurements were carried out in 300 dwellings in Kuwait using duplicate sets of charcoal detectors. Measurements were made at three different locations in the dwellings: living rooms, bedrooms, and basements. The results show that the radon concentration in the dwellings of Kuwait was found to vary in the range of 4.0-241.8 Bq m(-3) with a mean value of 32.8 Bq m(-3), and most values are confined within the range of 10-50 Bq m(-3) for all locations with few cases above the value of 100 Bq m(-3). Overall results show that the indoor radon concentration levels in Kuwait are relatively low, which is attributed to the use of air conditioning in summer and possible natural ventilation in winter. The radon concentration in basements was found to be relatively higher when compared to other rooms of the dwellings.     

Current status of programmes to measure and reduce radon exposure in Irish workplaces.

National legislation, which implements European Council Directive 96/29/EURATOM in Ireland, sets a reference level of 400 Bq m(-3) averaged over any 3 month period for radon exposure in the workplace and also empowers the Radiological Protection Institute of Ireland to direct employers to have radon measurements carried out. This legislation came into effect in May 2000. Radon measurements have already been completed in show caves and other underground workplaces. Between 1998 and 2001, over 33 800 individual radon measurements were carried out in all ground floor offices and classrooms in 3444 schools nationwide as part of a programme undertaken jointly with the Department of Education and Science. Where the average indoor radon concentration in one or more rooms exceeded 200 Bq m(-3), remedial measures were implemented. For concentrations up to 400 Bq m(-3) this involved increased ventilation while for higher concentrations an active sump was normally installed. The results of the survey, as well as the effectiveness of the different remedial strategies, are discussed. In the case of other above ground workplaces, different approaches have been adopted. As a first step, workplaces in two known high radon areas were directed to have radon measurements carried out. This programme had limited success because of problems in obtaining accurate workplace databases and a general lack of awareness on the part of employers of the issues involved. From a sample of 2610 employers directed to measure radon, only 408 actually completed measurements and 37 workplaces were identified as having average 3 month average radon concentrations above 400 Bq m(-3). A total of 1356 employers ignored all correspondence, some of which was sent by registered post and signed for on receipt. Current initiatives are focused on the provision of information and include newspaper advertising as well as publications aimed specifically at both employer and employee representative groups. The ability to provide accurate information that encourages both measurement and remediation is seen as central to an effective radon workplace programme.     

The potential risk from 222radon posed to archaeologists and earth scientists: reconnaissance study of radon concentrations, excavations, and archaeological shelters in the Great Cave of Niah, Sarawak, Malaysia

This reconnaissance study of radon concentrations in the Great Cave of Niah in Sarawak shows that in relatively deep pits and trenches in surficial deposits largely covered by protective shelters with poor ventilation, excavators are working in a micro-environment in which radon concentrations at the ground surface can exceed those of the surrounding area by a factor of > x 2. Although radon concentrations in this famous cave are low by world standards (alpha track-etch results ranging from 100 to 3075 Bq m(-3)), they still may pose a health risk to both excavators (personal dosemeter readings varied from 0.368 to 0.857 mSv for 60 days of work) and cave occupants (1 yr exposure at 15 h per day with an average radon level of 608 Bq m(-3) giving a dose of 26.42 mSv). The data here presented also demonstrate that there is considerable local variation in radon levels in such environments as these.     

Residential radon and lung cancer among never-smokers in Sweden.

In this study, we attempted to reduce existing uncertainty about the relative risk of lung cancer from residential radon exposure among never-smokers. Comprehensive measurements of domestic radon were performed for 258 never-smoking lung cancer cases and 487 never-smoking controls from five Swedish case-control studies. With additional never-smokers from a previous case-control study of lung cancer and residential radon exposure in Sweden, a total of 436 never-smoking lung cancer cases diagnosed in Sweden between 1980 and 1995 and 1,649 never-smoking controls were included. The relative risks (with 95% confidence intervals in parentheses) of lung cancer in relation to categories of time-weighted average domestic radon concentration during three decades, delimited by cutpoints at 50, 80, and 140 Bq m(-3), were 1.08 (0.8--1.5), 1.18 (0.9--1.6), and 1.44 (1.0--2.1), respectively, with average radon concentrations below 50 Bq m(-3) used as reference category and with adjustment for other risk factors. The data suggested that among never-smokers residential radon exposure may be more harmful for those exposed to environmental tobacco smoke. Overall, an excess relative risk of 10% per 100 Bq m(-3) average radon concentration was estimated, which is similar to the summary effect estimate for all subjects in the main residential radon studies to date.     

Occupational doses from radon in Spanish spas

Recent international recommendations have included exposure to natural radiation as one of the sources to monitor in certain occupationally exposed groups. Among those mentioned are workers in thermal spas, who may be exposed to high radiation doses due to the high concentration of radon in the indoor air of the spa. This paper presents the methodology and the results of an evaluation of radiation doses to the staff in different thermal spas in Spain. Different series of samples were collected and measurements made for the radon concentrations in water in 54 spas and in air in 20 spas. In six of the latter group, the air radon concentration was studied in different working areas occupied by the employees. The radon concentrations in water were between <2 and 775 x 10(3) Bq m(-3). The radon concentrations in air were between <10 and 5,200 Bq m(-3). The latter were used to estimate the dose received by each occupational group in the spa by taking into account the radon concentration in their main working area. By means of an exposure-dose conversion factor of 1.43 Sv per J h m(-3), the estimated effective doses were found to lie between 1 and 44 mSv y(-1). This upper limit is higher than the recommended annual limit of 20 mSv y(-1) for an occupational dose.     

The Castleisland Radon Survey-follow--up to the discovery of a house with extremely high radon concentrations in County Kerry (SW Ireland).

In July 2003, a house with a seasonally adjusted annual average radon concentration of 49 000 Bq m(-3) was identified near Castleisland in County Kerry (SW Ireland). The possibility that other houses with similar extreme radon concentrations could be present in the surrounding area triggered the setting up of a localised radon survey, the so-called 'Castleisland Radon Survey' (CRS). To this end, approximately 2500 householders living in four 10 x 10 km2 grid squares from the Irish grid closest to the town of Castleisland were invited to participate. Four hundred and eighteen householders responded to the invitation (17% response rate) and 383 home results were used for further analysis. In the 400 km2 encompassing the four studied grid squares, 14% of the homes were found to have a seasonally adjusted annual average radon concentration above the national reference level of 200 Bq m(-3) while 2% above 800 Bq m(-3). An average radon concentration of 147 Bq m(-3) was calculated. This can be compared with the average radon concentration of 98 Bq m(-3) calculated for the same four grid squares on the basis of 80 measurements carried out during the Irish National Radon Survey (NRS) which was conducted between 1992 and 1997. The fourth highest radon concentration (6184 Bq m(-3)) and three of the ten highest ever measured in Ireland were all identified during the CRS. This shows that localised and targeted radon surveys are an invaluable tool for the identification of homes at highest risk from high radon concentrations. Two of the four grid squares investigated during the CRS are currently designated as high radon areas (defined as areas where 10% or more of all houses are predicted to exceed 200 Bq m(-3)) as predicted by the NRS. A thorough statistical analysis of the CRS and NRS data was carried out and indicated that both datasets could be merged and used to refine the original NRS predictions. The results indicate that two of the four studied grid squares could potentially be redesignated. The practical feasibility and overall benefit of updating the Irish radon map in light of this analysis is described.     

The effectiveness of radon remediation in Irish schools

An advisory reference level of 200 Bq m(-3) and a statutory reference level of 400 Bq m(-3) apply to radon exposure in Irish schools. Following the results of a national survey of radon in Irish schools, several hundred classrooms were identified in which the reference levels were exceeded and a remediation program was put in place. This paper provides an initial analysis of the effectiveness of that remediation program. All remediation techniques proved successful in reducing radon concentrations. Active systems such as radon sumps and fan assisted under-floor ventilation were generally applied in rooms with radon concentrations above 400 Bq m(-3). These proved most effective with average radon reduction factors of 9 to 34 being achieved for radon sumps and 13 to 57 for fan assisted under-floor ventilation. Both of these techniques achieved maximum radon reduction factors in excess of 100. The highest average reduction factors were associated with the highest initial radon concentrations. Passive remediation systems such as wall and window vents were used to increase background ventilation in rooms with radon concentrations below 400 Bq m(-3) and achieved average radon reductions of approximately 55%. Following the installation of active remediation systems, the radon concentration in adjacent rooms, i.e., rooms in which the radon concentration was already below 200 Bq m(-3) and therefore did not require remediation, was further reduced by an average of 25%. The long-term effectiveness of a number of radon sump systems with at least three years operation showed no evidence of fan failures. This study showed an apparent increase in sump effectiveness with time as indicated by an increase in radon reduction factors during this period.     

Elevation of antioxidant enzymes in the clinical effects of radon and thermal therapy for bronchial asthma

An increased systemic production of oxygen-free radicals by activated inflammatory cells is thought to be involved in the pathophysiology of asthma. The aim of this study is to evaluate the clinical effects of radon and thermal therapy on asthma in relation to antioxidant enzymes and lipid peroxide. Radon and thermal therapy were performed once a week. All subjects went to a hot bathroom with a high concentration of radon, and nasal inhalation of vapor from a hot spring was performed for 40 min once a day under conditions of high humidity. The room temperature was 48 degrees C; the room radon concentration was 2,080 Bq/m3. Blood samples were collected at 2 h, 14, and 28 days after the first therapy. A blood sample also was collected before the first therapy (at body temperature and background radon level) to be used as the control. The forced expiratory volume in one second (%FEV1) was significantly increased 28 days after the first therapy. On day 28, the catalase (CAT) activity was significantly increased in comparison with the control. The superoxide dismutase (SOD) activity was significantly increased compared to the control after first inhalation. On days 14 and 28, the lipid peroxide level was significantly decreased in comparison with the control. In conclusion, the present pilot study has shown that radon and thermal therapy improved the pulmonary function of asthmatics by increasing the reduced activities of antioxidant enzymes.     

Using willingness to pay to evaluate the implementation of Canada's residential radon exposure guideline

BACKGROUND: The objective of this investigation was to determine the effectiveness of Canada's residential radon exposure guideline in influencing individuals' health protection decisions. METHOD: Homeowners with known exposure levels in a high residential radon area (Winnipeg, Manitoba) were surveyed to document what they had done and spent to reduce their exposure to radon. The 507 respondents were then re-surveyed to elucidate their response to hypothetical scenarios. Logistic regression was used to model risk reduction decisions as a function of exposure and other explanatory variables. RESULTS: Homeowners were only likely to have taken action to reduce exposure at levels exceeding 1,100 Bq/m3, well above Canada's guideline of 800 Bq/m3. However, when informed of the guideline, respondents indicated they would act at exposures of 702 Bq/m3. INTERPRETATION: The Canadian residential radon exposure guideline, as it has been implemented, has not effectively prompted homeowner actions to reduce exposures to radon.     

Nationwide survey of radon levels in Korea

A nationwide radon survey was conducted to provide data on the annual average indoor radon concentration in Korean homes. This survey also provided data on the variation of radon concentration with season, house type, and building age. The arithmetic mean (AM) of annual radon concentration in Korean homes was 53.4 +/- 57.5 Bq m(-3). The indoor radon concentration showed a lognormal distribution with a geometric mean (GM) and its standard deviation (GSD) of 43.3 +/- 1.8 Bq m(-3). The radon concentrations in the traditional Korean-style houses were about two times higher than those in apartments and row houses. The average annual outdoor radon concentration was 23.3 Bq m(-3). The average annual effective dose to the general public from radon was 1.63 mSv y(-1).     

Residential radon and risk of lung cancer in Eastern Germany

BACKGROUND: There is suggestive evidence that residential radon increases lung cancer risk. To elucidate this association further, we conducted a case-control study in Thuringia and Saxony in Eastern Germany during 1990-1997. METHODS: Histologically confirmed lung cancer patients from hospitals and a random sample of population controls matched on age, sex and geographical area were personally interviewed with respect to residential history, smoking, and other risk factors. One-year radon measurements were performed in houses occupied during the 5-35 years prior to the interview. The final analysis included a total of 1,192 cases and 1,640 controls. Odds ratios (OR) and 95% confidence intervals (CI) were estimated by logistic regression. RESULTS: Measurements covered on average 72% of the exposure time window, with mean radon concentrations of 76 Bq/m3 among the cases and 74 Bq/m3 among the controls. The smoking- and asbestos-adjusted ORs for categories of radon (50-80, 80-140 and >140 Bq/m*3, compared with 0-50 Bq/m3) were 0.95 (CI = 0.77 to 1.18), 1.13 (CI = 0.86 to1.50) and 1.30 (CI = 0.88 to 1.93). The excess relative risk per 100 Bq/m? was 0.08 (CI = -0.03 to 0.20) for all subjects and 0.09 (CI = -0.06 to 0.27) for subjects with complete measurements for all 30 years. CONCLUSIONS: Our data indicate a small increase in lung cancer risk as a result of residential radon that is consistent with the findings of previous indoor radon and miner studies.     

Increased lung cancer risk due to residential radon in a pooled and extended analysis of studies in Germany

Residential radon has been shown to be a risk factor for lung cancer in several studies-but with limited power in each single study. The data of two case-control studies performed during 1990-1997 in Germany and used for previous publications have been extended and pooled. Both studies have identical study designs. In total, data of 2,963 incident lung cancer cases and 4,232 population controls are analyzed here. One-year radon measurements were performed in houses occupied during the 5-35 y prior to the interview. Conditional logistic and linear relative risk regression was used for the analysis. Measurements covered on average 70% of the exposure time window, with an average radon exposure of 61 Bq m(-3). The smoking and asbestos-adjusted ORs were 0.97 [95% confidence interval (CI) 0.85 to 1.11] for 50-80 Bq m(-3), 1.06 (95% CI 0.87 to 1.30) for 80-140 Bq m(-3) and 1.40 (95% CI 1.03 to 1.89) for radon concentrations above 140 Bq m(-3), compared to the reference category <50 Bq m(-3). The linear increase in the odds ratio per 100 Bq m(-3) was 0.10 (95% CI -0.02 to 0.30) for all subjects and 0.14 (95% CI -0.03 to 0.55) for less mobile subjects who lived in only one home in the last 5-35 y. The risk coefficients generally were higher when measurement error in the radon concentrations was reduced by restricting the population. With respect to histopathology, the risk for small cell carcinoma was higher than for other subtypes. This analysis strengthens the evidence that residential radon is a relevant risk factor for lung cancer.     

Quality control of mitigation methods for unusually high indoor radon concentrations

The present study's objective was to control the quality of different mitigation methods for unusually high indoor radon (222Rn) concentrations of up to 274,000 Bq m(-3) in a village (Umhausen, 2,600 inhabitants) in western Tyrol, Austria. Five years after mitigation, five different remedial actions were examined on their quality by means of measuring indoor radon concentrations with charcoal liquid scintillation radon detectors and with a continuously recording AlphaGuard detector. Mitigation method in house 1--a mechanical intake and outlet ventilation system with heat exchanger in the basement, combined with a soil depressurization system--was characterized by long-term stability. With most favorable air pressure (+100 Pa) in the basement, mean basement radon concentrations in the winter were reduced from 200,000 Bq m(-3) to 3,000 Bq m(-3) by this method 5 y after mitigation. Acting against experts' instructions, the inhabitants had switched off the ventilation system most of the time to minimize power consumption although it had been proven that ventilation reduced mean basement radon concentration by a factor of about 3 in the winter and about 15 in the summer. Mitigation method in house 2-soil depressurization with two fans and loops of drainage tubes to withdraw radon from the region below the floor and outside the basement walls, and from soil below that part of the house with no basement-had been the most successful remedial measure until the winter of 1999 (i.e., 6 y after mitigation), when micro-cracks opened and consequently mean basement radon concentration increased from 250 Bq m(-3) to 1,500 Bq m(-3). Measures to block these microcracks and to minimize soil drying are being developed. Five years after mitigation, the remedial method used in house 3--a multilayer floor construction, where a fan was used to suck radon from a layer between bottom slab and floor-reduced winter mean radon concentration from 25,000 Bq m(-3) to 1,200 Bq m(-3), with the ventilation on and the basement door open. Mitigation method in house 4--a basement sealing technique--was unsuccessful with almost identical radon concentrations during all the five years since mitigation had started. Mitigation method in house 5--a waterproof basement technique especially for future homes--reduced mean basement radon concentration below 300 Bq m(-3) and mean ground floor radon concentration below 200 Bq m(-3), which is the Austrian action level for newly constructed buildings. These findings indicate that even in areas with extremely high radon concentrations, effective mitigation of indoor radon can be achieved provided that house-specific long-term, stable mitigation techniques are applied.     

Indoor radon in the region of Brussels

The indoor radon (222Rn) concentration has been measured by charcoal detectors in 278 buildings in the region of Brussels, Belgium. The correlation with the nature of the subsoil can be studied in detail thanks to the available geotechnical map. With a geometrical mean indoor radon concentration of 19 Bq m(-3), Brussels can be considered as generally unaffected by the radon problem. No value higher than 400 Bq m(-3) (the EU reference level for existing houses) was measured in an occupied room. However, two factors that may enhance the risk are identified: the absence of a basement or a ventilated crawl space, and the presence of loess, under the house. About one third of the houses without basements or ventilated crawl spaces built on loess show an indoor radon concentration above 200 Bq m(-3) (the EU reference level for new houses).     

Meta-analysis of residential exposure to radon gas and lung cancer

OBJECTIVES: To investigate the relation between residential exposure to radon and lung cancer. METHODS: A literature search was performed using Medline and other sources. The quality of studies was assessed. Adjusted odds ratios with 95% confidence intervals (CI) for the risk of lung cancer among categories of levels of exposure to radon were extracted. For each study, a weighted log-linear regression analysis of the adjusted odds ratios was performed according to radon concentration. The random effect model was used to combine values from single studies. Separate meta-analyses were performed on results from studies grouped with similar characteristics or with quality scores above or equal to the median. FINDINGS: Seventeen case-control studies were included in the meta-analysis. Quality scoring for individual studies ranged from 0.45 to 0.77 (median, 0.64). Meta-analysis based on exposure at 150 Bq/m3 gave a pooled odds ratio estimate of 1.24 (95% CI, 1.11-1.38), which indicated a potential effect of residential exposure to radon on the risk of lung cancer. Pooled estimates of fitted odds ratios at several levels of randon exposure were all significantly different from unity--ranging from 1.07 at 50 Bq/m3 to 1.43 at 250 Bq/m3. No remarkable differences from the baseline analysis were found for odds ratios from sensitivity analyses of studies in which > 75% of eligible cases were recruited (1.12, 1.00-1.25) and studies that included only women (1.29, 1.04-1.60). CONCLUSION: Although no definitive conclusions may be drawn, our results suggest a dose-response relation between residential exposure to radon and the risk of lung cancer. They support the need to develop strategies to reduce human exposure to radon.     

Lowering the UK domestic radon Action Level to prevent more lung cancers--is it cost-effective?

Case studies have shown that radon gas can accumulate within domestic properties at sufficiently high levels that it can cause lung cancer, and recent studies have suggested that this risk remains significant below the UK domestic Action Level of 200 Bq m(-3). Raised radon levels can be reduced by engineering measures, and it has been shown that domestic radon remediation programmes in UK Affected Areas can result in reduced risks to the population and can be cost-effective. We consider here the benefits and costs of the domestic radon remediation programme in Northamptonshire, UK, and consider the implications for that programme of reducing the UK Action Level below its present value. A radon remediation programme based on an Action Level above 200 Bq m(-3) will cost less and will target those most at risk, but will be less cost-effective and will lead to higher residual dose and greater risk of cancer in the remaining population. Reducing the Action Level below 200 Bq m(-3) will prevent more cancers, but at significantly higher cost. It will also be less cost-effective, because remediation of a significant number of houses with moderate radon levels will provide only a modest health benefit to occupants. Overall, a completed radon remediation programme of the type implemented in Northamptonshire is most cost-effective for an Action Level between 200 and 300 Bq m(-3). The implications for future health policy are discussed.     

湛江市部分住房室内外空气中氡浓度测定

采用活性炭吸附闪烁法测定了湛江市部分住房室内、外空气中的平衡当量氡浓度。结果表明 ,住房室内、外空气中平衡当量氡浓度平均值分别为 2 5 2 7Bq/m3 和 14 6 8Bq/m3 ,室内空气中平衡当量氡浓度显著高于室外 (P <0 0 1) ;不同装饰材料的住房室内空气中平衡当量氡浓度各有差异 ,其中以花岗岩为装饰材料的住房室内平衡当量氡浓度 (31 18Bq/m3 )为最高 ,水泥地板住房 (14 71Bq/m3 )最低     

Eyeglass lenses for personal radon dosimetry

Eyeglass lenses are commonly composed of allyl-diglycol carbonate (CR-39), an alpha-particle detecting plastic, thus making such lenses personal radon dosimeters. Samples of such lenses have been etched to reveal that radon and radon progeny alpha tracks can be seen in abundance, and sensitivities have been calibrated in radon chambers as a primary calibration and with a uranium-based source of alpha particles as a convenient secondary standard. Natural, environmental (fossil) track densities ranged from 3,000 to 25,000 cm(-2) for eyeglasses that had been worn for various times from 1 to nearly 5 y. Average radon concentrations to which the wearers were exposed are inferred to be in the range 20 to 130 Bq m(-3) (0.5 to 3.5 pCi L(-1)). Procedures for consistent, meaningful readout are described.