快速特异性免疫治疗对哮喘患者血清免疫球蛋白E的影响

目的通过观察哮喘患者快速特异性免疫治疗前后血清免疫球蛋白E(IgE)的变化,探讨其治疗机制。方法 160例血清总IgE(tIgE)>100 kU/L,屋尘螨特异性IgE(sIgE)>0.72 kU/L的哮喘患者,随机分为治疗组80例,对照组80例,治疗组用屋尘螨变应原浸液快速特异性免疫治疗,对照组吸入布地奈德,在治疗前后抽静脉血采用免疫荧光技术检测血清tIgE和sIgE。结果 两组治疗后患者血清tIgE和sIgE均降低,(治疗组P<0.01,对照组P<0.05),治疗组明显低于对照组(P<0.01)。结论快速特异性免疫治疗能有效降低患者血清tIgE和sIgE值,这可能为特异性免疫治疗作用机制之一。     

飞行人员花粉症的快速免疫治疗

快速免疫疗法是采用快速的脱敏方法对过敏性疾病进行免疫治疗的一种方法，即在很短的时间内达到有效的维持剂量。我们于1990年9月～1992年12月为35名飞行人员花粉症患者进行了快速免疫治疗，结果如下。一、临床资料35例花粉症患者均为男性现役空军飞行人员，25～49岁。病史最短3年，最长17年。发病时均有典型症状（鼻阻、喷嚏、清涕、鼻痒）和体征。按全国统一标准”’记分与分级。35例中“”者18例，‘”者且2例，“＋”者5例；病情属“重”级19例，“中”级11例，“轻”级5例。每年发病时间基本一致，有32例在每年7～9月发病，有3例从6月…     

针刺治疗慢性荨麻疹的疗效及其对组胺和IgE的影响

目的探讨针刺治疗慢性荨麻疹的疗效及其对组胺(HA)和IgE的影响。方法对30例慢性荨麻疹患者进行针刺治疗,并记录治疗前后的症状积分和检测患者的血清HA及总IgE水平。结果经14次治疗后,11例患者治愈,显效9例,有效2例,总有效率为66.67%。治疗后HA及总IgE水平均较治疗前明显下降(P<0.05)。结论针刺是治疗慢性荨麻疹的一种安全、有效的方法。     

快速脱敏疗法对真菌致敏性哮喘的疗效及IgE,ECP的影响

目的　对真菌致敏性哮喘进行快速脱敏治疗 ,观察其疗效 ,对比治疗前后血清总IgE ,ECP的变化。 方法　对洛阳农村地区 14 0例真菌哮喘患者随机分为两组 ,A组 70例 ,B组 70例。A组采用常规治疗。B组病人在常规治疗基础上采用北京协和医院提供的多价霉菌浸出液做快速脱敏治疗 ,注射浓度从 1:10 5(W /V)开始 ,以 0 .1,0 .2 ,0 .4,0 .8ml剂量递增 ,上午及下午各 1次皮下注射 ,尔后以 1:10 4 ,1:10 3,1:10 2浓度递增 ,8d即可达维持量 ,从第 9日开始进行维持治疗 ,用 1:10 2 浓度每周 1次 0 .5ml皮下注射 ,1年后全部结束。治疗前 1周及 1年疗程结束后 1周抽 2ml静脉血测定治疗前后总IgE ,ECP ,并进行疗效判断。结果　血清总IgE(KU/L)A组治疗前为 (165 .5 0± 41.0 2 )KU/L ,治疗后为 (160 .3 0± 2 5 .3 2 )KU/L ,前后对比无明显差异 (P >0 .0 5 ) ;B组治疗前为 (170 .65± 40 .2 0 )KU/L ,治疗后为 (75 .3 0± 2 0 .48)KU/L ,前后对比差异显著 (P <0 .0 1)。血清ECPA组治疗前为 (19.2 4± 3 .18) μg/L ,治疗后为 (17.2 5± 3 .98) μg/L ,前后对比无明显差异 (P >0 .0 1) ;B组治疗前为 (18.2 4± 4.0 8) μg/L ,治疗后为 (10 .3 3± 2 .67) μg/L ,前后对比差异显著 (P <0 .0 1)。疗效判断A组临床控制 2 2例     

飞行人员花粉症继发航空性中耳炎84例免疫治疗

我们于1989年9月～1995年9月共收治飞行人员花粉症126例，其中继发航空性中耳炎84例均接受快速免疫治疗。1临床资料1．l一般情况84例均为男性现役飞行人员，年龄24～52岁，平均36岁；平均飞行时间1520h。患者除有打喷嚏、流涕、鼻阻等典型花粉症表现外，飞行中有耳痛、耳闷及听力下降等症状。1．2治疗方法根据变应原皮肤试验结果，取其中阳性反应最强的变应原制剂（标准变应原由北京协和医院提供），通过皮下注射快速免疫法’“进行特异性快速免疫治疗。于次年花粉症发病季节后复诊，记录其临床症状的变化情况，再次进行变应原测定、咽鼓管…     

哮喘患者特异性免疫治疗中检测NK细胞活性的临床意义

目的探讨在哮喘尘螨特异性免疫治疗中自然杀伤(NK)细胞活性的临床意义。方法分别检测尘螨过敏的哮喘患者85例,正常对照组30例成人NK细胞活性,并对哮喘患者进行尘螨特异性免疫治疗,治疗前后检测其NK细胞活性及总IgE。结果哮喘患者的NK细胞活性比正常对照组显著减少(P<0.01),总IgE显著降低(P<0.01)。结论NK细胞参与哮喘的发病过程,是一种重要的免疫调节细胞。特异性免疫疗法有助于NK细胞活性增强,降低总IgE水平,从而减少哮喘的发作。     

氯雷他定联合西替利嗪在荨麻疹中的治疗效果观察

目的 探讨氯雷他定联合西替利嗪在荨麻疹中的治疗效果.方法 选取2008年1月～2010年12月于本院进行治疗的80例荨麻疹患者为研究对象,将其随机分为对照组(氯雷他定组)40例和观察组(氯雷他定联合西替利嗪组)40例,后将两组患者治疗一周及两周总有效率、不良反应发生率及治疗前后的血清HA、ECP、总IgE、5-HT水平...     

特应性皮炎患者血清 IgE 水平检测临床意义

目的:探讨特应性皮炎(AD)与血清总IgE和抗原特异性IgE水平的关系。方法:选择AD患者44例及正常体检者(对照组)22例,采用MAST-CLA过敏原检测仪和MAST检测试剂盒,定量测定血清总IgE含量;采用血清过敏原特异性IgE抗体体外检测试剂盒,检测抗原特异性IgE水平。结果:AD患者血清总IgE浓度显著高于对照组,与AD病情严重程度呈正相关,抗原特异性IgE阳性率81.8%。结论:血清总IgE和抗原特异性IgE测定有助于判定特应性皮炎病情严重程度和活动度。     

变应性鼻炎与非变应性鼻炎的鉴别诊断及其疗效观察

目的探讨变应原检测在临床诊断变应性鼻炎与非变应性鼻炎患者的重要性及变应性鼻炎的治疗方法。方法2011年3月～2013年4月临床症状和体征表现判断符合了鼻炎诊断的750例患者,经抽血做变应原的筛查,对检测结果进行分析,并对患有变应性鼻炎的患者采取特异性免疫治疗。结果初诊750例患者有500例的总IgE〉60ku／L,Hx2与phadiatop阳性,与此同时,还伴有该临床症状,可以确诊是变应性鼻炎;250例病患的总IgE〈60ku／L,H）（2与phadiatop阴性,虽然他们具备变应性鼻炎相关的临床症状表现,但应诊断为非变应性鼻炎。变应性鼻炎使用安脱达屋尘螨变应原制剂轮流注射,效果显著。结论临床上有一部分符合变应性鼻炎地相关症状患者（约33．3％）变应原检测为阴性,应该十分重视对变应原的检查,为正确诊断和进行治疗而提供科学有效的指导。近年来,针对变应性鼻炎,特异性免疫治疗方法,注射剂量及疗程更加规范。效果显著。     

特异性免疫治疗花粉症54例临床观察

特异性免疫治疗(以往又称脱敏治疗)具有治疗病因,兼有预防作用.花粉过敏病因明确、特异性较强.我们采用特异性花粉抗原对54例花粉过敏者进行免疫治疗,随访观察3年,报告如下.临床资料1.诊断依据:典型的花粉症症状和体征,显著的发病季节性;花粉抗原皮肤试验阳性(结果在两个加     

Changes in mucosal and humoral atopic-related markers and immunoglobulins in elite cyclists participating in the Vuelta a España

Atopic-related factors, humoral and mucosal immunoglobulins (Ig), and cortisol were measured in 17 professional cyclists competing in the 2003 Vuelta a Espa?a (a three-week multi-stage race). Venous blood and saliva samples were obtained the morning before the start of the race (T0), on the first rest day after 10 days of racing (T1), and before the start of the last stage after 21 days of racing (T2). Atopic-related factors, IgE, eosinophil cationic protein (ECP), and eosinophils, were significantly altered during the race. Serum IgE (T1: + 10 %) and ECP (salivary, T1: 113 % and serum, T2: 155 %) were significantly increased, while eosinophils (T1: - 32 %, T2: - 55 %) were significantly lower, than pre-race levels. Salivary sIgA secretion rate was significantly decreased at T2 (- 36 %). Pearson product-moment correlations revealed a modest correlation between salivary sIgA and salivary ECP (T1: r = 0.30; T2: r = 0.48; p < 0.01). Serum IgM, total IgG, IgG1, IgG2, IgA levels, at T1 and T2, and cortisol at T2, were significantly lower than pre-race levels. In conclusion, the elevation in IgE and ECP suggests an up-regulation of atopic-related factors in professional cyclists participating in the Vuelta a Espa?a. The correlation between salivary sIgA and salivary ECP indicates a role for sIgA in mediating mucosal inflammation. The alterations in Ig levels may indicate Ig isotype switching. An increasing state of hormonal fatigue may have influenced the observed immune alterations.     

Total allergen-specific IgE antibodies in the serum and skin reactivity induced by a mixture of pollens in allergic individuals

Skin puncture test is fast and precise method for revealing IgE-mediated allergen hypersensitivity. In the diagnosis of pollinosis and other atrophic diseases, expensive tests for revealing the allergen-specific IgE antibodies in the serum are frequently inaccessible. The subject of our investigation was how and if the skin test with mixture of pollen could replace the expensive in-vitro method in diagnostic procedure. In 41 patients with pollinosis were performed skin tests by the mixture of pollen of grass, weeds and trees (produced by Torlak Institute, Belgrade) and determined the concentration of overall and allergen-specific IgE serum (EIA, enzyme immuno-assay, Pharmacia Uppsala; RAST Phadesim, multidisk, Pharmacia). Obtained results pointed out that the sex of investigated subjects did not influence the size of skin reaction in case when the pollen mixture (grass, weed or trees) was used. With high significance rate was established that in skin puncture test, just the size of the reaction, defined by 5 mm papule or larger, indicated the hypersensitive person. We consider that this can be taken as the diagnostic criterion and replace in-vitro method for determination of allergen-specific IgE antibodies in serum. Also, overall IgE of serum positively correlated (p < 0.05) with the size of skin reaction only in the persons with extremely high concentrations (RAST class 4) allergen-specific IgE antibodies in serum.     

Alterations of IgE immunoglobulin concentration in the serum after megavoltage radiotherapy with large-field technique in patients with Hodgkin's disease

In patients with Hodgkin's disease, the serum IgE concentrations were determined with the PRIST radioimmunoassay before commencing and after ending systemic megavoltage radiotherapy. 57 patients showed mainly raised serum IgE concentrations before radiotherapy. After the end of radiotherapy, 44 patients were investigated, and a highly significant fall of the IgE concentration was shown in the serum following the radiotherapy. The decrease of IgE concentration was most pronounced in the patients in whom large areas of irradiation were necessary or who displayed B symptoms. In a proportion of the patients, the IgE concentration in the serum could be measured once more six and twelve months after the end of radiotherapy. Although the IgE concentrations rose again after radiotherapy, the initial values before the beginning of radiotherapy had not yet been reached again even twelve months after treatment in 21 patients investigated. A long-lasting immunosuppressant effect of radiotherapy on the serum IgE concentration could thus be demonstrated which corresponds to the effect on the serum IgM concentration described earlier.     

复合抗原在哮喘免疫治疗中的作用

目的:观察结核菌素纯蛋白衍生物(PPD)与特异性过敏原组成的复合抗原免疫对哮喘的治疗作用。方法:将非急性发作期的哮喘患者90例,随机分为传统治疗组(30例)、复合抗原治疗A组和B组(各30例),3组在维持原控制药基础上,传统治疗组用特异性过敏原进行脱敏治疗,每周皮下注射一剂量单位至最佳维持剂量后,每2周注射一次维持量,直至哮喘完全缓解1年;复合抗原治疗A组和B组用PPD+特异过敏原组成的复合抗原进行脱敏治疗,初始浓度和递增速度与传统组相同,维持阶段:A组每2周注射一次维持量,B组每4周注射一次维持量,直至哮喘完全缓解一年。用ELISA定量检测试剂盒检测治疗前后患者血清及痰中IL-4、IL-5、IFN-γ及IgE表达水平。结果:传统治疗组和复合抗原治疗2组治疗后血清及痰中IL-4、IL-5、IgE表达水平均显著下降,IFN-γ表达水平则明显升高,与治疗前比较均相差显著(P<0.05),但复合抗原A组和B组之间差异无显著性(P>0.05)。结论:复合免疫法能明显抑制因过敏原接触导致的以TH2应答为主的免疫反应,调节TH1/TH2的平衡,其对治疗支气管哮喘有显著疗效。     

美能治疗花粉症临床观察

目的探讨美能注射液治疗花粉症疗效。方法56例花粉症患者随机分为两组,治疗组32例,对照组24例,治疗组除应用盐酸西体利嗪片,维生素C片,钙剂等治疗外,应用美能注射液40 m l用5%葡萄糖注射液250 m l稀释后,静脉滴注,1次/d,连用1周;对照组不用美能注射液,其余治疗与治疗组相同。结果治疗组花粉症患者治疗前症状总评分为10.40±2.24,1周后总评分为2.5±0.14,治疗前、后比较有显著性差异(P<0.01);对照组花粉症患者治疗前症状总评分为10.20±2.56,1周后总评分为5.48±1.21,治疗前、后比较有显著性差异(P<0.01),两组显效率相比,治疗组为63.3%明显高于对照组的41.7%(P<0.01)。结论美能注射液治疗花粉症疗效显著,无明显副作用。     

复方木尼孜其对女性迟发性痤疮血清睾酮的影响及疗效观察

目的观察复方木尼孜其对女性迟发性痤疮血清睾酮的影响及疗效。方法将128名痤疮患者随机分为两组。治疗组(A组)65例口服复方木尼孜其颗粒。对照组(B组)63例口服一清胶囊和维生素B6,治疗时间为28 d。另设62例相应年龄段非痤疮患者作对照组(C组)。于治疗前后采用电化学发光免疫分析法检测其卵泡期血清睾酮水平,并进行疗效评价。结果 A组和B组的总有效率分别为89.2%和61.9%,差异有统计学意义(P<0.05)。A组治疗前血清睾酮水平较C组明显升高(P<0.05),而治疗后血清睾酮水平与C组相比差异无统计学意义(P>0.05)。结论女性迟发性痤疮发病的主要原因与雄激素分泌增多有关。复方木尼孜其颗粒具有抗雄激素作用,对女性迟发性痤疮的疗效确切,且无明显不良反应。