景洪哥纳香根的化学成分研究

目的研究植物景洪哥纳香Goniothalamus cheliensis根的化学成分。方法采用硅胶、ODS以及Sephadex LH-20等柱色谱方法分离、纯化化合物,根据理化性质和光谱数据鉴定化合物结构。结果从景洪哥纳香根中分离并鉴定了7个化合物,其中4个为苯乙烯内酯类化合物,分别鉴定为乙酰哥纳香呋吡喃酮A(acetylgoniofupyrone A,1)、哥纳香二醇(goniodiol,5)、8-表哥纳香三醇(8-epi-goniotriol,6)、心瓣哥纳香丁内酯(cardiobutanolide,7),另外3个化合物分别为原儿茶酸(protocatechuic acid,2)、松属素(pinocembrin,3)、松脂素(pinoresinol,4)。结论化合物1为呋喃并吡喃型苯乙烯内酯,且为新化合物,命名为哥纳香乙酰酯A。     

景洪哥纳香的化学成分研究Ⅱ

 目的分离鉴定景洪哥纳香化学成分。方法用乙醇提取、硅胶柱层析分离,通过IR,NMR及MS光谱分析法确定结构。结果分得5个化合物,分别鉴定为8-羟基-7-苯基-2,6-二氧双环［3,3,1］壬烷-3-酮(Ⅰ)、10-氨基-4-羧基-3,8-二甲氧基菲-1-羧酸内酰胺(Ⅱ)、10-氨基4,8-二羟基-3-甲氧基菲-1-羧酸内酰胺(Ⅲ)、β-谷甾醇(Ⅳ)和β-胡萝卜苷（Ⅴ)。结论分得的5个化合物均为首次从该植物中发现,化合物Ⅲ为新化合物。     

景洪哥纳香叶中的苯乙烯内酯类化合物

目的：研究景洪哥纳香叶的抗肿瘤活性苯乙烯内酯类成分。方法：采用硅胶色谱柱分离，理化性质、光谱数据鉴定结构。结果：分离鉴定7个化合物，其中4个为苯乙烯内酯：（＋）-isoaltholactone（1），goniodiol-7-monoacetate（2），leiocarpin A（3），goniodiol（4），另3个为松乔素（pinocembrin，5），胡萝卜苷（daucosterol，6）和豆甾醇（stigmasterol，7）。结论：化合物1-4体外有抗肿瘤活性，化合物1,2、3为首次从该植物分离得到。     

景洪哥纳香胺的分离和结构鉴定

从景洪哥纳香Goniothalamus cheliensis树皮中分离得到4个化台物,经理化性质和光谱分析鉴定为十六酸(I)、5,6-2H-6-苯乙烯吡喃-2-酮(Ⅱ)、(SR,6S,7R,8R)-7,羟基-8苯基四氢呋喃并[3,2-b]-2H-吡喃-2-酮(Ⅱ)、景洪哥纳香胺(Ⅳ)。化合物I为首次从该植物中分得,化合物Ⅳ为从哥纳香属中发现的一种新结构类型的生物碱,为一新化合物。     

Beesiosides A-F, six new cycloartane triterpene glycosides from Beesia calthaefolia.

Six new cycloartane triterpene glycosides (1-6), beesiosides A-F, were isolated from whole plants of Beesia calthaefolia, and their structures were elucidated on the basis of extensive NMR experiments and chemical methods. Beesiosides A-F were assigned as (20S,24R)-epoxy-9,19-cyclolanostane-3beta,16beta,18,25-tetraol-3-O-beta-D-xylopyranoside (1), (20S,24R)-epoxy-9,19-cyclolanostane-3beta,12beta,16beta,18,25-pentaol-3-O-beta-D-xylopyranoside (2), (20S,24R)-epoxy-9,19-cyclolanostane-3beta,12alpha,16beta,18,25-pentaol-3-O-beta-D-xylopyranoside (3), (20S,24R)-16beta-acetoxy-20,24-epoxy-9,19-cyclolanostane-3beta,12alpha,18,25-tetraol-3-O-beta-D-xylopyranoside (4), (20S,24R)-epoxy-9,19-cyclolanostane-3beta,15alpha,16beta,18,25-pentaol-3-O-beta-D-xylopyranoside (5), and (20S,24R)-16beta-acetoxy-20,24-epoxy-9,19-cyclolanostane-3beta,12beta,25-triol-3-O-beta-D-xylopyranoside (6), respectively.     

Phyllanemblinins A-F, new ellagitannins from Phyllanthus emblica.

Six new ellagitannins, phyllanemblinins A-F (1-6), were isolated from Phyllanthus emblica, along with 30 known tannins and related compounds. Their structures were determined by spectral and chemical methods. Phyllanemblinins A (1) and B (2) were confirmed to be ellagitannins having a tetrahydroxybenzofuran dicarboxyl group and a hexahydroxydiphenoyl group, respectively, by chemical synthesis from furosin (8). Phyllanemblinin C (3) has a new acyl group at the glucose 2,4-positions and is structurally related to chebulagic acid. Phyllanemblinins D (4), E (5), and F (6) were found to be positional isomers of neochebuloyl 1(beta)-O-galloylglucose.     

Comazaphilones A-F, azaphilone derivatives from the marine sediment-derived fungus Penicillium commune QSD-17

Chemical investigation of Penicillium commune QSD-17, a fungus isolated from a marine sediment sample collected in the southern China Sea, yielded six new azaphilone derivatives, namely, comazaphilones A-F (1-6). The structures of these compounds were established on the basis of spectroscopic analysis. Attempts to define the absolute configuration of these azaphilones through investigation of Mosher's esters failed, possibly due to steric crowding at C-6 and C-7 and due to the degradation of these azaphilone derivatives under the reaction conditions. The inhibitory activities of the six azaphilones against four bacteria, one pathogenic fungus, and seven tumor cell lines were evaluated. Compounds 3-5 displayed potent inhibitory activity against several of these bacteria, while compounds 4-6 showed cytotoxic activity against human pancreatic tumor cell line SW1990. The preliminary SAR results indicated that the double bond at C-10 and the location of the orsellinic acid unit at C-6 in these azaphilones are important for the antibacterial activity and cytotoxicity, respectively. This is the first report of the isolation of azaphilone derivatives from a marine sediment-derived fungus.     

景洪暗罗中一个新的对映桉烷型倍半萜

目的 研究了番荔枝科暗罗属植物景洪暗罗Polyalthia cheliensis树叶的化学成分。方法 通过液—液分配、大孔吸附树脂脱色、硅胶柱色谱和RP—18反相柱色谱分离，运用质谱、核磁共振氢谱、碳谱及二维碳氢相关谱。结果 从其树叶干粉(6kg)的90％乙醇提取物中分离鉴定了一个桉烷型倍半萜：1α—乙酰氧基对映桧醇(8mg)。结论 这是一个新化合物。     

Bitungolides A-F,new polyketides from the Indonesian sponge Theonella cf. swinhoei

Six new polyketides, bitungolides A-F (1-6), have been isolated from the Indonesian sponge Theonella cf. swinhoei and their structures elucidated by spectroscopic data and X-ray diffraction analysis. The bitungolides are a new class of Theonella metabolites that inhibit dual-specificity phosphatase VHR.     

Acutifolins A-F, a new flavan-derived constituent and five new flavans from Brosimum acutifolium.

Acutifolin A (1), a novel constituent with a bicyclo[3.3.1]non-3-ene-2,9-dione ring, acutifolins B-F (2-6), five new flavans, and three known flavans were isolated from the bark of Brosimum acutifolium, a Brazilian folk medicine ("Mururé"). Their structures were elucidated by spectroscopic methods, including 2D NMR.     

Caudatosides A-F: new iridoid glucosides from Citharexylum caudatum

Six new iridoid glucosides, caudatosides A-F (2-7), and one known iridoid glucoside, 5-deoxypulchelloside I (1), were isolated from the fruits of Citharexylum caudatum. The structures of these compounds were elucidated by various one- and two-dimensional NMR techniques as well as high-resolution negative-ion ESIMS. Other plant parts were extracted on a small scale, and TLC of these extracts revealed the presence of the same iridoids.     

Two new styryl lactones, 9-deoxygoniopypyrone and 7-epi-goniofufurone, from Goniothalamus giganteus.

Two new styryl lactones, 9-deoxygoniopypyrone [1] and 7-epi-goniofufurone [3], and a known styryl lactone, goniodiol [5], were isolated from the stem bark of Goniothalamus giganteus. The structures were elucidated by ir, ms, 1H-nmr, 13C-nmr, and 1H-1H COSY spectra; the relative configurations were determined by X-ray crystallographic analysis. Unlike goniopypyrone [2] and goniofufurone [4], neither of the new styryl lactones 1 and 3 showed significant bioactivities to human tumor cells. However, goniodiol [5] showed significant and selective cytotoxicity against human lung tumor cells (A-549).     

Antitumor agents. 233. Lantalucratins A-F, new cytotoxic naphthoquinones from Lantana involucrata

In a continuing study to isolate novel antitumor agents from rainforest plants, three new isopropenylfurano-beta-naphthoquinones, designated lantalucratins A (1), B (2), and C (3), and three new isoprenyl-alpha-naphthoquinones, designated lantalucratins D (4), E (5), and F (6), were isolated from Lantana involucrata. Their structures were determined on the basis of NMR and X-ray crystallographic analyses. Compounds 1 and 2 showed cytotoxic activities against various human tumor cell lines, including drug-resistant variants, with IC50 values of 1.0-4.9 microM.     

Ulongamides A-F,new beta-amino acid-containing cyclodepsipeptides from Palauan collections of the marine cyanobacterium Lyngbya sp

Six new beta-amino acid-containing cyclic depsipeptides, termed ulongamides A-F (1-6), have been isolated from collections of apratoxin-producing cyanobacteria from Palau. Their planar structures have been determined by NMR spectroscopic techniques. The absolute stereochemistry of the hydroxy acid and all the alpha-amino acid-derived units was ascertained to be S by chiral HPLC analysis of degradation products. The stereochemistry of the beta-amino acid moiety, 3-amino-2-methylhexanoic acid, was established by advanced Marfey analysis of the acid hydrolyzates and found to be 2R,3R in compounds 1-3 but 2S,3R in compounds 4-6. All compounds except 6, which lacks an aromatic amino acid moiety, were weakly cytotoxic against KB cells.     

Resveratrol derivatives from the roots of Vitis thunbergii

Investigating the constituents of the roots of Vitis thunbergii led to the isolation of four new resveratrol derivatives, vitisinols A-D (1-4), together with (+)-epsilon-viniferin, (-)-viniferal, ampelopsin C, miyabenol A, (+)-vitisin A, and (+)-vitisin C. The structures of these 10 compounds were established by spectroscopic (NMR and MS) analyses. All of the isolated compounds, except 1, were tested for their antiplatelet and antioxidative activities.     

Trigonosins A-F, daphnane diterpenoids from Trigonostemon thyrsoideum

Phytochemical study of the roots of Trigonostemon thyrsoideum led to the isolation of four new oxygenated daphnane-type diterpenoids, trigonosins A-D (1-4), and two new modified daphnanes, trigonosins E and F (5 and 6). The structures and relative configurations were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR experiments. All compounds isolated were evaluated for their cytotoxicity against HL-60, A549, and MCF-7 human cancer cell lines.     

Gymnasterkoreaynes A-F,cytotoxic polyacetylenes from Gymnaster koraiensis

Six new polyacetylenes, gymnasterkoreaynes A-F (1-6), were isolated from the roots of Gymnaster koraiensis, together with 2,9,16-heptadecatrien-4,6-diyn-8-ol (7) and 1,9,16-heptadecatriene-4,6-diyn-3,8-diol (8), by bioassay-guided fractionation using the L1210 tumor cell line as a model for cytotoxicity. The structures of compounds 1-6 were established spectroscopically, which included 2D NMR experiments. Gymnasterkoreaynes A-F (1-6) are linear diacetylenes and are structurally related to falcarinol, panaxynol, panaxydiol, and panaxytriol. Of the compounds isolated, gymnasterkoreaynes B (2), C (3), F (6), and 1,9,16-heptadecatrien-4,6-diyn-3,8-diol (8) exhibited significant cytotoxicity against L1210 tumor cells with ED(50) values of 0.12-3.3 microg/mL.     

Dihydroagarofuran derivatives from the dried roots of Tripterygium wilfordii

Five new sesquiterpene derivatives, including dihydroagarofuran pyridine macrolides 1-4 and dihydroagarofuran ester 18, and 13 known dihydroagarofuran derivatives were isolated from the aqueous EtOH extract of the dried roots of Tripterygium wilfordii. An in vitro antiherpetic activity assay indicated that compounds 11 and 17 displayed weak and moderate inhibition against herpes simplex virus type II, respectively.     

Eremophilane-type sesquiterpene derivatives from the roots of Ligularia lapathifolia

Six new highly oxygenated eremophilane-type sesquiterpene derivatives (1-6), including a norbisesquiterpene, were isolated from an extract of the roots of Ligularia lapathifolia, and their structures were elucidated by spectroscopic methods. The structure of 1 was confirmed by single-crystal X-ray crystallography. In addition, the cytotoxicity of compounds 1, 2, 3, 5, and 6 was evaluated against selected cancer cell lines, including human stomach carcinoma (MGC-803), human hepatoma (HEP-G2), and murine sarcoma (S-180) cell lines.     

9,19-cyclolanostane derivatives from the roots of Actaea pachypoda

Phytochemical investigation of the chemical constituents of the roots of Actaea pachypoda afforded 12 9,19-cyclolanostane type triterpenoids, including the new 7,8-dihydroactaeaepoxide 3-O-beta-d-xylopyranoside (1), 12-deacetoxyactaeaepoxide 3-O-beta-d-xylopyranoside (2), and 12beta-acetoxycimigenol (3). Their structures were determined by spectroscopic and chemical methods.