Effect of diquertin on platelet content of cyclic nucleotides

The effects of diquertin on the content of cyclic nucleotides in human platelets was studied. Diquertin in a concentration of 5 mM increased the content of cAMP and cGMP in native and thrombin-activated platelets probably due to inhibition of phosphodiesterase. Increasing the concentration of diquertin above 5 mM did not potentiate this effect. The antiaggregation effect of diquertin was probably associated with the increase in platelet levels of cyclic nucleotides. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny,Vol. 128, No. 9, pp. 267–269, September, 1999     

Apparatus for one-stage determination of redox potential,free oxygen concentration,and local circulation in a tissue

An apparatus for the combined investigation of tissue bioenergetics is described: the redox potential, free oxygen concentration, and local blood flow can be determined.Gor'kii Research Institute of Traumatology and Orthopedics. (Presented by Academician of the Academy of Medical Sciences of the USSR B. A. Korolev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 8, pp. 1015–1016, August 1976.     

Role of prostaglandins and cyclic nucleotides in the mechanism of development of drug-induced thrombocytopenia

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 354–356, April, 1993     

Effect of enteral oxygen therapy on liver function in acute degeneration

The effect of enteral oxygen therapy was studied in rats with acute degeneration of the liver caused by CCl₄. Intragastric injection of oxygen foam reduced the severity of poisoning and led to more rapid and complete recovery of the intensity of bile secretion, synthesis of primary bile acids, and their conjugation with amino acids, and improved the stabilizing properties of the bile.Department of Pharmacology, Ternopol' Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR S. V. Anichkov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 10, pp. 419–421, October, 1977.     

Quantitative continuous measurement of partial oxygen pressure on the skin of adults and new-born babies

Summary It is possible to perform continuous quantitativeP _{O 2} measurements on vasodilated skin by means of surface Pt electrodes according to Clark when the electrode is fixed to the skin with a synthetic plastic material and in situ calibration is performed. A new in situ calibration of theP _{O 2} electrode is described. At first the skinP _{O 2} increases with O₂ inspiration. After perfusion stop the skinP _{O 2} shows a linear decrease because of the skin respiration, down to aP _{O 2} at which hemoglobin liberates chemically bound O₂. As thisP _{O 2} value of hemoglobin is known it is possible to use it for calibrating the electrode. TheP _{O 2} of normal skin is about 0–7 Torr. After vasodilation obtained by rubbing with a nicotinic acid derivate (Finalgon^®, Anasco, Wiesbaden),P _{O 2} increases to a mean value of 38.1 (±8.1) Torr (n=77). Under these conditions, skinP _{O 2} reaches arterial values never in adults and rarely in new-born babies.Part of the results have been reported during the Workshop on Oxygen Transport in Tissue, 19–22 July, 1971, in Dortmund and at the 4. Deutsche Kongress für Perinatale Medizin, 4–6 Nov. 1971, in Berlin. The study was carried out with partial support from the German Research Council (DFG).     

Arginine-vasopressin effect on the content of cyclic nucleotides, cellular protein phosphorylation, and adhesion to plastic of peritoneal mouse macrophages

Intracellular concentration of cAMP and phosphorylation of 15 kD protein in cultured mouse peritoneal macrophages are increased in the presence of arginine-vasopressin. Vasopressin, decreases adhesion of macrophages to plastic. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 7 pp. 80–83, July 1998     

Effect of cyclic AMP on cells of experimental lymphatic leukemia L-5718

The effect of cyclic AMP (cAMP), dibutyryl-cAMP, and theophylline (an inhibitor of phosphodiesterase, the enzyme converting adenosine-3, 5-monophosphate into adenosine-5-monophosphate) on the intensity of proliferation (as reflected in the increase in the nucleic acid content in the culture), DNA synthesis (thymidine-H³ incorporation), and transplantation properties (ability to repopulatein vivo) of leukemic cells of strain L-5178 was studied. The experiments showed that cAMP in a concentration of 0.8 mM inhibits thymidine-H³ incorporation considerably, retards proliferation, and reduces the transplantability of the leukemic cells. Theophylline and dibutyryl-cAMP have comparatively weak ability to inhibit DNA synthesis and the proliferative activity and transplantation properties of the cells.Laboratory of Biochemistry and Laboratory of Experimental Therapy of Leukemias, Central Institute of Hematology and Blood Transfusion, Ministry of Health of the USSR. Laboratory for the Search for New Antibiotics, All-Union Research Institute for the Search for New Antibiotics, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR K. V. Bunin.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 81, No. 3, pp. 365–367, March, 1976.     

Effect of repeated partial hepatectomy on cell division in the regenerating rat liver

Repeated partial hepatectomy (PH), performed 24 h after a 70% PH, had the following effect on the mitotic cell cycle in the regenerating rat liver: it delayed (by about 2 h) the cells in the G₂ period, left the S period almost unchanged, and delayed the cells for 6–8 h in the G₁ period. A mock repeated operation had a similar effect. This indicates that the influence of the repeated PH on the mitotic cell cycle in the regenerating liver is due to operation stress. Additional stimulation of division by repeated PH affects the character of the regeneration process as a whole.Laboratory of Chemical Factors of Regulation of Growth and Cell Division, Institute of Biological and Medical Chemistry, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR V. N. Orekhovich.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 10, pp. 488–491, October, 1977.     

Mechanism of the cytolytic effect of streptolysin O: Influence of scavengers of active forms of oxygen and complexones on erythrocyte hemolysis

Translated fromByulleten' Eksperimental'noi Biologii iMeditsiny, Vol. 115, N ^o 3, pp. 277–278, March, 1993     

Non-invasive multiwavelength photoplethysmography under low partial pressure of oxygen

A reduction in partial pressure of oxygen in the environment may be caused by a gain in altitude, which reduces the atmospheric pressure; it may also be caused by the carbon dioxide generated from breathing in an enclosed space. Does inhaling oxygen of lower partial pressure affect the oxygen-carrying function of haemoglobin in vivo? This study uses non-invasive multiwavelength photoplethysmography to measure the effects that inhaling this type of oxygen can have on the plethysmography of the appendages of the body (fingertips). The results indicate that under low partial pressure of oxygen, be it the result of a gain in carbon dioxide concentration or altitude, the change in visible light absorption is the biggest for short wavelengths (approximately 620 or 640?nm) near deoxyhaemoglobin, which has higher absorption coefficient. Moreover, increasing carbon dioxide concentration from 5000 to 10,000?ppm doubly reduces the absorption rate of these short wavelengths.     

Role of hemoglobin affinity for oxygen in the activation of lipid peroxidation during fever

Major parameters of oxygen transport and lipid peroxidation are measured in the blood of rabbits with fever, and it is found that hemoglobin affinity for oxygen is decreased while free-radical processes are activated in this state. The data are subjected to multiple correlation analysis and the matrix of paired correlation coefficients obtained for the measured parameters is presented. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o , pp. 44–47, January, 1996 Presented by K. V. Sudakov, Member of the Russian Academy of Medical Sciences     

Myoglobin concentration and partial oxygen pressure in muscle tissue during allergic reactions

The effect of sensitization to horse serum, brucellas, and BCG, and also the effect of allergic reactions induced by these antigens on the partial pressure of oxygen and myoglobin concentration in muscle tissue were studied. The myoglobin concentration was found to be reduced during the formation of increased sensitivity but only in the case of sensitization by living microbial strains. Allergic reactions of immediate and delayed types caused a decrease in the myoglobin concentration both in the myocardium and skeletal muscles. The partial pressure of oxygen in the skeletal muscles was lowered.Central Research Laboratory, Alma-Ata Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR N. D. Beklemishev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 87, No. 5, pp. 452–454, May, 1979.