中药内服联合脊柱局部正骨推拿治疗颈腰腿痛效果分析

〔摘 要〕 目的：分析中药内服联合脊柱局部正骨推拿治疗颈腰腿痛的效果。方法：选取佛山市中医院 2018 年 1 月至 2020 年 1 月期间收治的 360 例颈腰腿痛患者，按照数字表法均分为对照组和观察组，各 180 例。对照组用中药内服治疗， 观察组用中药内服联合脊柱局部正骨推拿治疗，比较两组患者的治疗效果和生活质量评分。结果：观察组患者治疗总有效 率为 97.22 %，高于对照组的 88.89 %，差异具有统计学意义（P ＜ 0.05）；治疗前两组患者的生活质量评分比较，差异无 统计学意义（P ＞ 0.05）；治疗后观察组患者生活质量评分均优于对照组，差异具有统计学意义（P ＜ 0.05）。结论：中药 内服联合脊柱局部正骨推拿治疗增加了颈腰腿痛患者的治疗步骤，提高了患者的治愈率。     

针灸联合中药熏蒸治疗颈肩腰腿痛的效果

〔摘 要〕 目的：观察对颈肩腰腿痛患者行以针灸联合中药熏蒸治疗的效果。方法：选取遂溪县人民医院 2017 年 5 月 至 2019 年 12 月期间收治的 50 例颈肩腰腿痛患者,将其随机分为对照组（25 例）行以针灸治疗,观察组（25 例）行以 针灸联合中药熏蒸治疗,观察比较两组患者治疗前后的症状评分、腰椎功能评分、肢体运动功能评分及治疗总有效率。 结果：治疗前两组患者的疼痛评分、功能受限评分、肿胀评分比较,差异无统计学意义（P ＞ 0.05）;治疗后两组患者的 疼痛评分、功能受限评分、肿胀评分均有不同程度下降,且观察组低于对照组,差异具有统计学意义（P ＜ 0.05）。治疗 前两组患者的腰椎功能评分比较,差异无统计学意义（P ＞ 0.05）;治疗后两组患者的腰椎功能评分均有不同程度的提 高,且观察组高于对照组,差异具有统计学意义（P ＜ 0.05）。治疗前两组患者的肢体运动功能评分比较,差异无统计学 意义（P ＞ 0.05）;治疗后两组患者的肢体运动功能评分均有不同程度的提高,且观察组高于对照组,差异具有统计学意 义（P ＜ 0.05）。观察组患者治疗总有效率为 100 %,高于对照组的 84 %,差异具有统计学意义（P ＜ 0.05）。结论： 对颈肩腰腿痛患者来说,针灸联合中药熏蒸治疗效果确切,可以改善临床症状,还可以改善腰椎功能以及肢体运动功能。     

针灸联合推拿治疗神经根型颈椎病的临床效果观察

〔摘 要〕 目的：观察分析针灸联合推拿治疗神经根型颈椎病的临床效果。 方法：选取 2009 年 1 月至 2019 年 1 月广东 祈福医院收治的 100 例神经根型颈椎病患者,按照数字随机表法分为对照组和观察组,各 50 例,对照组患者应用针灸治 疗,观察组患者应用针灸联合推拿治疗,比较分析两组患者的临床效果、治疗前后中医证候积分以及治疗前后的颈肩痛伴 上肢放射痛和颈肩压痛评分情况。 结果：治疗前两组患者的颈肩痛伴上肢放射痛和颈肩压痛评分比较,差异无统计学意义 （P ＞ 0.05）;治疗后观察组患者颈肩痛伴上肢放射痛和颈肩压痛评分均显著低于对照组,差异具有统计学意义（P ＜ 0.05）。 观察组患者临床治疗有效率为 96.0 %,明显高于对照组的 82.2 %,差异具有统计学意义（P ＜ 0.05）。治疗前两组患者的 中医证候积分比较,差异无统计学意义（P ＞ 0.05）;治疗后观察组患者中医证候积分均显著低于对照组,差异具有统计 学意义（P ＜ 0.05）。 结论：针灸联合推拿治疗神经根型颈椎病临床效果显著,且能够减轻患者的颈肩痛伴上肢放射痛和 颈肩压痛。     

平衡针灸联合康复训练治疗颈肩腰腿痛的临床效果

〔摘 要〕 目的：观察平衡针灸联合康复训练治疗颈肩腰腿痛的临床效果。方法：选取广州市番禺区何贤纪念医院 2018 年 6 月至 2020 年 6 月期间收治的 120 例颈肩腰腿痛患者,按照是否开展平衡针灸联合康复训练将患者分为对照组（60 例：康 复训练）与观察组（60 例：平衡针灸联合康复训练）,比较两组患者治疗效果。结果：治疗前两组患者的肩关节 Neer 评分、 日本骨科协会评估治疗分数（JOA）评分以及 Lysholm 评分比较,差异无统计学意义（P ＞ 0.05）。治疗后观察组患者的 Neer评分、JOA评分以及Lysholm评分均优于对照组,差异具有统计学意义（P＜0.05）;观察组患者治疗总有效率为96.97 %, 高于对照组的 70.00 %,差异具有统计学意义（P ＜ 0.05）。结论：颈肩腰腿痛患者给予平衡针灸联合康复训练疗效显著, 优于单纯康复训练。     

中药内服联合中医手法复位治疗 闭合性前臂骨折的临床疗效

〔摘 要〕 目的：观察中药内服联合中医手法复位治疗闭合性前臂骨折的临床疗效。 方法：选取漳州市漳浦中医院 2022 年 1 月至 2022 年 12 月收治的 66 例闭合性前臂骨折患者，按照是否实施中药内服联合中医手法复位治疗将患者分为 对照组（中医手法复位治疗）与观察组（中药内服联合中医手法复位治疗），每组 33 例，比较两组患者治疗效果。 结果：观察组患者治疗后 3 d、7 d、14 d 的视觉模拟评分法（VAS）评分低于对照组，差异具有统计学意义（P ＜ 0.05）；观察组 患者并发症总发生率低于对照组，差异具有统计学意义（P ＜ 0.05）；治疗 3 个月后，观察组患者尺偏角、掌倾角、前臂旋前、 前臂旋后度均高于对照组，差异具有统计学意义（P ＜ 0.05）；观察组患者总有效率高于对照组，差异具有统计学意义 （P ＜ 0.05）。 结论：闭合性前臂骨折患者中药内服联合中医手法复位治疗效果优于单一中医手法复位治疗。     

针灸结合中药热敷治疗颈肩腰腿疼痛的短期疗效观察

〔摘 要〕 目的：分析针灸结合中药热敷治疗颈肩腰腿疼痛的短期疗效。方法：选取郴州市第一人民医院 2017 年 1 月 至2019年6月期间收治的100例颈肩腰腿疼痛患者,随机分为观察组及对照组,各50例。观察组实施针灸结合中药热敷治疗, 对照组实施常规针灸治疗,观察比较两组疼痛患者日常生活能力（ADL）评分、视觉模拟评分法（VAS）评分和治疗效果、 并利用焦虑自评表（SAS）及抑郁自评表（SDS）进行评分,判断患者情绪状态。结果：观察组患者治疗总有效率为 98.00 %, 高于对照组的 70.00 %,差异具有统计学意义（P ＜ 0.05）;治疗前两组患者的 VAS 评分、ADL 评分、SAS 评分及 SDS 评 分比较,差异无统计学意义（P ＞ 0.05）;经治疗观察组患者的 VAS 评分、SAS 评分及 SDS 评分均优于对照组,ADL 评 分高于对照组,差异均具有统计学意义（P ＜ 0.05）。结论：在颈肩腰腿疼痛患者治疗理中应用针灸结合中药热敷治疗, 有效提升患者的治疗依从性,减轻患者的疼痛,改善患者的日常生活能力。     

小针刀治疗颈肩肌筋膜疼痛综合征的疗效观察

〔摘 要〕 目的：研究小针刀治疗颈肩肌筋膜疼痛综合征的临床疗效。方法：选择化州市中医院 2020 年 1 月至 2021 年 1 月收治的颈肩肌筋膜疼痛综合征患者 80 例作为研究对象,按照数字随机表法分为对照组和观察组,各 40 例。 对照组实施常规针灸治疗,观察组采用小针刀治疗,观察两组患者临床治疗效果,两组患者治疗前后血清 5– 羟色胺 （5–HT）、β– 内啡肽（β–ET）水平以及疼痛视觉模拟评分法（VAS）评分、巴氏量表（BI）评分以及颈椎功能障 碍指数（NDI）评分。结果：观察组患者的治疗有效率为 97.5 %,明显高于对照组的 82.5 %,差异具有统计学意义 （P ＜ 0.05）。治疗前,两组患者血清 5–HT、β–ET 水平比较,差异无统计学意义（P ＞ 0.05）;治疗后,观察组患者 β–ET 水平明显高于对照组,5–HT 水平明显低于对照组,差异具有统计学意义（P ＜ 0.05）。治疗前,两组患者 VAS、BI 评分以及 NDI 评分比较,差异无统计学意义（P ＞ 0.05）;治疗后,观察组患者的 BI 评分明显高于对照组, VAS 评分及 NDI 评分明显低于对照组,差异具有统计学意义（P ＜ 0.05）。结论：治疗颈肩肌筋膜疼痛综合征采取 小针刀治疗,有利于患者恢复颈肩部功能,明显减轻疼痛。     

动静结合按揉天宗穴治疗颈型颈椎病的临床疗效

〔摘 要〕 目的：观察动静结合按揉天宗穴治疗颈型颈椎病的疗效。 方法：选取开封市中医院 2020 年 6 月至 2022 年 6 月 就诊的 60 例颈型颈椎病患者,随机分为对照组与观察组,每组 30 例。对照组患者采用传统推拿手法治疗,观察组患者在 传统推拿手法治疗后予以动静结合按揉天宗穴治疗,比较两组患者治疗前后视觉模拟评分法（VAS）评分、颈椎功能障碍 指数（NDI）、治疗效果和预后情况。 结果：治疗后,两组患者 VAS 评分均降低,且观察组患者 VAS 评分均低于对照组, 差异具有统计学意义（P ＜ 0.05）;治疗后,两组患者 NDI 评分均降低,且观察组患者 NDI 评分均低于对照组,差异具有 统计学意义（P ＜ 0.05）;观察组患者总有效率高于对照组,差异具有统计学意义（P ＜ 0.05）。 结论：动静结合法按揉天 宗穴可有效缓解患者疼痛症状,改善生活质量,达到颈型颈椎病快速康复的目的。     

经络推拿联合中药熏蒸对颈肩腰腿痛患者疼痛及关节功能的影响

目的：观察经络推拿联合中药熏蒸治疗颈肩腰腿痛的临床疗效。方法：选取90 例颈肩腰腿痛患者,随机分为治疗组和对照组各45 例。对照组予以中药熏蒸治疗,治疗组给予经络推拿联合中药熏蒸治疗。2 组均治疗3 个月,比较2 组临床疗效、中医证候积分、腰椎功能、疼痛程度及血液流变学情况。结果：治疗组临床总有效率为88.89%,对照组为71.11%,2 组比较,差异有统计学意义（P＜0.05）。治疗前,2 组疼痛、肿胀、肢体功能受限等中医证候评分比较,差异无统计学意义（P＞0.05）;治疗后,2 组中医证候各项评分均较治疗前下降（P＜0.05）,且治疗组各项评分均低于对照组（P＜0.05）。治疗前,2 组日本骨科协会关节功能评分量表（JOA）、Oswestry 功能障碍指数（ODI）、视觉模拟评分法（VAS） 评分比较,差异无统计学意义（P＞0.05）;治疗后,2 组JOA 评分均较治疗前上升（P＜0.05）,ODI、VAS 评分较治疗前下降（P＜0.05）;且治疗组JOA 评分高于对照组（P＜0.05）,ODI、VAS 评分低于对照组（P＜0.05）。治疗前,2 组血浆黏度、红细胞体积、纤维蛋白原比较,差异无统计学意义（P＞0.05）;治疗后,2 组血浆黏度、红细胞体积、纤维蛋白原均较治疗前下降（P＜0.05）,且治疗组上述指标均低于对照组（P＜0.05）。结论：经络推拿联合中药熏蒸治疗颈肩腰腿痛疗效肯定,能够显著缓解患者临床症状和疼痛,提高关节功能,改善血液流变学状态。     

精针刀配合中药熏蒸治疗寒湿凝滞型 神经根型颈椎病临床疗效

〔摘 要〕 目的：观察精针刀配合中药熏蒸治疗寒湿凝滞型神经根型颈椎病的临床疗效。 方法：选取 2021–2022 年 云浮市人民医院收治的寒湿凝滞型神经根型颈椎病患者 55 例,随机分为观察组（27 例,给予精针刀配合中药熏蒸）和 对照组（28 例,单纯给予精针刀）进行治疗,比较两组患者的临床疗效、治疗前后肩颈部症状评分、上肢、手指症状 评分、椎间孔挤压试验评分以及视觉模拟评分法（VAS）评分。 结果：观察组患者治疗总有效率高于对照组,差异 具有统计学意义（P ＜ 0.05）;治疗后两组患者肩颈部症状、上肢及手指症状、椎间孔挤压试验、VAS 评分均较治疗前 下降,且治疗后观察组患者肩颈部症状、上肢及手指症状、椎间孔挤压试验、VAS 评分均低于对照组,差异均具有 统计学意义（P ＜ 0.05）。 结论：精针刀配合中药熏蒸比单纯精针刀治疗寒湿凝滞型神经根型颈椎病有更好的临床疗效。     

The Evolutional Development of Traditional Chinese Medicine (TCM) Outside China Mainland: Challenges,Training, Practice, Research, and Future Development

This overview has provided an account of evolutional changes of an experience-based traditional medical practice of traditional Chinese medicine (TCM) towards modernisation to keep up with recent advances in analytical and biomedical sciences, and information technology, which may help readers to understand why applying biomedical research methodology to TCM modernisation, while maintaining the experience-based concepts, principles and heritage of TCM’s personalised health and medical approaches in balancing body’s functions with physical and mental harmony when facing environmental changes, can contribute to gain global appreciation and acceptance of TCM in healthcare. It is envisaged that such future development and integration with biomedicine-based main-stream medicine (MSM) in practice will provide valuable medical care in the development of future personalised health and medicine as well as TCM product development.     

Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Galium mexicanum

Ethnopharmacological relevance

To study the potential benefit of the traditional Mexican medicinal plant Galium mexicanum Kunth (Rubiaceae). Hexane, chloroform, and methanol extracts as well as various fractions from these extracts were tested to determine antibacterial, antifungal, antiparasitic or anti-inflammatory activities in vitro.

Materials and methods

Aerial parts of the plant were extracted with various solvents and fractionated accordingly. Their antibacterial and antifungal activities were assessed on nine bacterial and four fungal strains. Leishmania donovani was used as a protozoan strain for antiparasitic activity. The anti-inflammatory activity of the compounds was investigated by measuring the secretion of interleukin-6 when macrophages were exposed to lipopolysaccharide.

Results

Various extracts and fractions obtained from this plant exhibit antibacterial, antifungal, antiparasitic, and anti-inflammatory activities. Of special interest was the hexane fraction HE 14b, which show antibacterial (ranging between 67 and 666 μg/ml) and antifungal (at concentrations of 333 μg/ml) activities. Also the hexane fraction HE 5 exhibited antiparasitic activity (at concentrations of 260 μg/ml), whereas the methanol fraction ME 13-15 showed a potent anti-inflammatory activity when compared to dexamethasone. Chemical analyses of the chloroform extract show the presence of triterpenes, saponins, flavonoids, sesquiterpene lactones, and glucosides, but no tannins were detected in the assayed extract.

Conclusions

The benefit of Galium mexicanum as a traditional medicinal plant was confirmed using antibacterial and antifungal assays in vitro. We also report for the first time, and to the best of our knowledge, antiparasitic and anti-inflammatory activities of this plant.     

血清CEA、CA-125、CA-153、CA-199、CA242联合检测对消化道肿瘤诊断的临床意义

目的：探讨血清CEA、CA-125、CA-153、CA-199、CA242联合检测对消化道肿瘤诊断的临床意义。方法：采用酶联免疫吸附法（ELISA法）检测130例恶性消化道肿瘤患者（食管癌32例、胃癌34例、胰腺癌30例、大肠癌34例）血清CEA、CA-125、CA-153、CA-199、CA242水平。结果：表明上述四种消化道肿瘤的血清CEA、CA-125、CA-153、CA-199、CA242与对照组比较结果统计学上均有显著性差异（P〈0．01）。除胰腺癌之外，其余3种消化道肿瘤五项肿瘤标记物联合检测的阳性率均显著高于单一标志物检测（P〈0．05），分别为食管癌62．5％、胃癌85．2％、大肠癌82．3％。结论：血清CEA、CA-125、CA-153、CA-199、CA242联合检测可以显著提高消化道恶性肿瘤诊断的敏感性。     

分段开窗旷置结合切扩挂线置管引流术治疗复杂性肛瘘的安全性多中心临床研究

目的探讨分段开窗旷置结合切扩挂线置管引流术治疗复杂性肛瘘的安全性。方法采用随机对照、多中心平行试验的设计方法,将240例复杂性肛瘘患者随机分为2组:试验组120例采用分段开窗旷置结合切扩挂线置管引流术治疗,对照组120例采用切开挂线术治疗,比较2组肛门直肠结构和功能改善情况。结果术后试验组肛门失禁和肛门狭窄、移位、变形等发生率低;肛管直肠压力、直肠容量感觉功能和直肠肛管反射强度降低幅度对照组明显大于试验组。结论分段开窗旷置结合切扩挂线置管引流术治疗复杂性肛瘘可控性与安全性好,值得作为肛瘘手术的新规范化术式在临床中推广应用。     

方剂剂量与君臣佐使关系初探

以2 022个方剂为研究对象, 围绕其中中药剂量及君臣信息, 计算中药的相对剂量, 并进行方剂内的中药相对剂量排名, 利用相关关系分析方法, 进行"中药相对剂量排名"与"君臣佐使情况"之间的相关关系假设检验。探讨中药剂量与中药"君、臣、佐、使"之间的关系。中药相对剂量排名与君、臣地位存在相关关系, 且为负相关。中药相对剂量排名与佐、使地位不存在相关关系。某味中药在处方中如果相对剂量高, 越有可能成为该方的主要药物, 而某味中药是否为该方的佐药或使药与相对剂量无关。     

A prospective,randomized, double-blind,multicenter comparative study on the safety and efficacy of Celecoxib and GCSB-5, dried extracts of six herbs,for the treatment of osteoarthritis of knee joint

Ethnopharmacological relevance

This prospective, randomized, double-blind, multicenter study compared the efficacy and safety of Celecoxib and GCSB-5, a new product from extracts of six herbs, for the treatment of knee osteoarthritis.

Materials and methods

A total of 198 eligible patients were randomly assigned to the Celecoxib group (n=99 patients) or the GCSB-5 group (n=99 patients) for the 12-week study. The amount of change and percentage of the change in Western Ontario and McMaster Universities (WOMAC) Arthritis Index from the baseline, the change in pain on walking by visual analogue scale (VAS), physician's global assessment on response to therapy (PGART) by five point Likert scale, and the amount of rescue medicine taken were used as parameters for efficacy. Adverse drug reactions (ADRs) were carefully investigated.

Results

The WOMAC score improved in both the Celecoxib group and GCSB-5 group by 20.5 and 21.3 (P=0.79). The percentage of the change in WOMAC score were −42.0% and −38.9% (P=0.54). The pain VAS score decreased by 29.9 and 27.9 (P=0.58). The responders by PGART were 95.3% and 93.8% (P= 0.66), and the median amount of rescue medicine taken were 2.0 and 6.5 tablets (P=0.06). The incidence of ADRs were 31.3% and 21.2% (P=0.11). The most common ADRs were gastrointestinal system related; 17.2% in GCSB-5 group and 22.2% in Celecoxib group. Any severe ADR was not observed in either group.

Conclusions

The result of this study supports that GCSB-5 is comparable to Celecoxib in terms of the efficacy and safety for the treatment of osteoarthritis of knee joint.     

Comparative biological study of roots,stems, leaves,and seeds of Angelica shikokiana Makino

Ethnopharmacological relevance

Angelica shikokiana has been used as a health food for its anticancer, anti-inflammatory, antibacterial, antiallergic, and blood vessel dilating effects in Japan. It can also be used to prevent and treat hepatitis, diabetes, hyperlipidemia, and arteriosclerosis.

Aim of the study

The present study was designed to compare the biological activities such as melanin synthesis inhibitory, anti-allergy, anti-lipase, anti-bacterial, anti-oxidant, and neuroprotective activities of different parts of the plant that may justify the use of this plant in folk medicine.

Material and methods

The roots, stems, leaves and, seeds of Angelica shikokiana were separately extracted with water and ethanol. Each extract was examined for melanin synthesis inhibitory and anti-allergy activity on B16-melanoma and RBL-2H3 cells using IgE and A23187 as a stimulant for β-hexosaminidase release, respectively. We also evaluated the inhibition of two enzymes, lipase and acetylcholine esterase, and of the bacterial growth of two species, Escherichia coli and Staphylococcus aureaus. The anti-oxidant activity was determined using oxygen radical anti-oxidant capacity, ORAC assay and its relation to the phenolic content was estimated using the Folin–Ciocalteu method. Besides, the protective effect of the extracts against H₂O₂-induced oxidative stress in mouse neuroblastoma, Neuro-2A cells was investigated.

Results

The most active extract exhibiting melanin synthesis inhibition (63%) and at the same time with low cytotoxicity (15%) was the ethanol extract of roots at 20 µg/ml, followed by the ethanol extract of stems (57% inhibition, 5% cytotoxicity). On the other hand, the highest inhibitions of β-hexosaminidase release were recorded for the ethanol extract of leaves with IC₅₀ value of 6.89 µg/ml followed by the water extract of the seeds and leaves with IC₅₀ value of 78.32 and 88.44 µg/ml, respectively. For anti-lipase assay, ethanol extracts of the stems and roots showed the strongest inhibition with IC₅₀ values of 204.06 and 216.24 µg/ml, respectively. None of the examined extracts showed any activity against Escherichia coli. while the ethanol extract of the roots and stems showed moderate inhibition for Staphylococcus aureus with minimum inhibitory concentration of 400 µg/ml. Ethanol extract of the roots showed only 30% inhibition of acetylcholine esterase enzyme. The results of anti-oxidant, phenolic content and protective effect against H₂O₂-induced cytotoxicity assays showed highly correlated data. Ethanol extract of the stems (ORAC value of 1.08 µmol Trolox/ mg and phenolic content 44.25 μg GAE/mg) increased the cell viability of H₂O₂-treated Neuro-2A cells by 28%.     

Anti-inflammatory and cicatrizing activities of thymol, a monoterpene of the essential oil from Lippia gracilis, in rodents

Ethnopharmacological relevance

Lippia gracilis Schauer (Verbenaceae) has long been recognized in folk medicine as a medicinal plant. The essential oil of Lippia gracilis has antimicrobial activity and is used externally to treat cutaneous diseases, burns, wounds, and ulcers. Recently, our research group demonstrated that the essential oil of Lippia gracilis leaves possesses antinociceptive and anti-inflammatory actions and its major component identified was thymol. The objective of this study was to assess the anti-inflammatory and wound healing activities of thymol in rodents.

Materials and methods

For the anti-inflammatory analysis the paw oedema and peritonitis models were used, followed by the assessment of the mieloperoxidase (MPO) activity, total cell counting, and histological analysis. The animals were treated (i.p., n=6/group) with thymol (10, 30, and 100 mg/kg), dexamethasone (2 mg/kg), or vehicle (1% Tween 80). In order to assess the wound healing potential, thymol was vehiculated into collagen-based dressing films and a biological wound healing test was conducted. The retraction index of the wounds and histological analysis were performed on the 3rd, 7th, 14th, and 21th days, split into three groups: undressed wounds (CTR), dressed with collagen-based films (COL), and dressed with collagen-based containing thymol (COLTHY) films.

Results

Thymol reduced significantly the oedema (100 mg/kg, P<0.001) and, besides, diminished the influx of leukocytes to the injured area (10, 30, and 100 mg/kg), according to the assessment of MPO activity (P<0.001), total cell count (P<0.05), and histological analysis. Wounds dressed with COLTHY films showed significantly bigger wound retraction rates (7 and 14 day, P<0.05) and improved the granulation reaction, as well provided better collagenization density and arrangement during wound healing.

Conclusions

This study suggests that thymol is a promising compound to be used in treatment of inflammatory processes as well as wound healing. The pharmacological actions of Lippia gracilis in popular medicine practices may be related, at least in part, to the presence of thymol in the essential oil.     

Sedative, vasorelaxant, and cytotoxic effects of convolvulin from Ipomoea tyrianthina

Aim of the study

Ipomoea tyrianthina has been used in Mexican traditional medicine as a mild purgative, for the treatment of nervous disorders, and against tumors. In this study, the effect of convolvulin (an ether-insoluble resin glycoside) from the root of Ipomoea tyrianthina on: Central Nervous System; as spasmolytic and vasodilator; cytotoxic against cancer cell lines is evaluated.

Materials and methods

Convolvulin isolated from the root of Ipomoea tyrianthina (IT-EM) was tested on pentylentetrazole induced seizures, pentobarbital-induced hypnosis, release of GABA and glutamic acid, isolated rat aorta and ileum rings, and against Caco-2 and KB cell lines.

Results

IT-EM increased the hypnotic effect induced by pentobarbital and the release of GABA in brain cortex of mice, but did not protect mice against pentylenetetrazole-induced convulsions. IT-EM produced a significant vasodilator effect in concentration- and endothelium-dependent manners on isolated rat aorta, but did not inhibit significantly contractions on rat ileum, colon, and jejune rings. IT-EM showed cytotoxic activity against nasopharyngeal carcinoma KB cell line.

Conclusions

Convolvulin (IT-EM) from Ipomoea tyrianthina has sedative effect, vasorelaxant effect in concentration- and endothelium-dependent manners, and cytotoxic activity against nasopharyngeal carcinoma KB cell line.     

Ethnopharmacological survey of medicinal plants used by traditional health practitioners in Thanchi,Bandarban Hill Tracts,Bangladesh

Ethnopharmacological relevance

There is very limited information regarding plants used by traditional healers in Bandarban Hill Tracts (BHT), Bangladesh for treating general as well as complex ailments. Current study provides significant ethnopharmacological information, both qualitative and quantitative on medical plants in BHT. Aim of the study This study aimed to collect, analyze and evaluate the rich ethnopharmacologic knowledge on medicinal plants in Thanchi, BHT and attempted to identify the important species used in traditional medicine. Further analysis was done by comparison of the traditional medicinal use of the plants with the available scientific literature data.

Materials and methods

The field survey was carried out in a period of several years in Thanchi upazilla of Bandarban districts, Bangladesh. A total of 53 Traditional Health Practitioners (THPs) were interviewed through open-ended and semi structured questionnaire. The collected data were analyzed qualitatively and quantitatively. This ethnomedicinal knowledge was compared against the available scientific literature for reports of related uses and studies of phytochemical compounds responsible for respective ailments.

Results

A total of 84 species of plants, mostly herbs, belonging to 42 families were identified for the treatment of 70 types of ailments. These ailments were categorized into 16 categories. Leaves were the most frequently used plant parts and juice is the mode of preparation of major portions of the plant species. The most common mode of administration was oral ingestion and topical application. Informant consensus factor (F_ic) values of the present study reflected the high agreement in the use of plants in the treatment of digestive system and respiratory system disorders among the informants. Diseases of the digestive system had highest use-reports and 3 species of plants, namely Centella asiatica (L.) Urban. (dysentery), Justicia gendarussa L. (asthma) and Ocimum tenuiflorum L. (coughing) had the highest fidelity level (FL) of 100%. Clerodendrum viscosum Vent., Achyranthes aspera L and Justicia gendarussa L. showed the highest relative importance (RI) value of 1.86. According to use value (UV) the most important species were Ocimum tenuiflorum L. (2.24) and Achyranthes aspera L. (2.15).

Conclusion

Priority should be given for further phytochemical investigation to plants that scored highest FL, F_ic, UV or RI values, as such values could be considered as good indicator of prospective plants for discovering new drugs. Since knowledge regarding traditional medicine being vulnerable day by day so traditional medicine in school curricula should be incorporated so that younger people could appreciate its usefulness. Also counseling of THPs and gardening of medicinal plants should be taken into consideration immediately in order to smooth continuance and extension of traditional medical knowledge and practice for ensuring safe and effective therapy.