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1.
Masoud Foroutan Sahar Dalvand Shahram Khademvatan Hamidreza Majidiani Hamidreza Khalkhali Sajjad Masoumifard Gebraeil Shamsaddin 《Transfusion and apheresis science》2017,56(4):544-551
Background
The risk of transfusion transmitted leishmaniasis (TTL) from apparently healthy persons or asymptomatic individuals, should not be ignored. Lack of a comprehensive review, encouraged us to design a systematic review with meta-analysis approach to assess the prevalence of Leishmania infection in healthy blood donors.Methods
For this purpose, 6 English databases (PubMed, Scopus, Web of Sciences, Science Direct, EMBASE and CINAHL) were browsed from January 1990 to July 2016.Results
Due to significant heterogeneity, the random-effects model was used (I2 = 98.04% and 94.68%, for serological and molecular methods, respectively). A total of 496 papers were found through searching in which 17,816 apparently healthy blood donors were examined for Leishmania infection. The weighted overall prevalence of Leishmania infection in this group was estimated 4% (95% CI = 2–7) and 8.7% (95% CI = 4.2–14.3) using serological and molecular methods, respectively.Conclusions
High serological prevalence does not justify widespread donor screening. Leukodepletion filters would substantially decrease the risk of TTL, hence they are potentially proposed in endemic areas specifically for high-risk recipients. To better enlighten the epidemiological aspects of Leishmania infection in blood donors, it is suggested to perform high-level stewardship and more precise studies with regard to involved risk factors. 相似文献2.
C. Kajetanek R. Rousseau K. Makridis M. Sanchez C. Delin P. Djian 《Journal de Traumatologie du Sport》2017,34(1):37-43
Background
Anterior cruciate ligament (ACL) rupture is a common pathology. Risk factors include significant tibial slope. The purpose of this study was to determine whether this relationship is observed in recurrent rupture of the ACL. Our hypothesis was that significant tibial slope is a risk factor for rupture.Material and methods
We reviewed at two years follow-up 386 ligamentoplasties performed from 2000 to 2012. There were 20 recurrent ruptures in this series (5.2%). These patients, mean age 21 ± 6.3 years, underwent 3D EOS goniometry to measure the medial and lateral tibial slope (MTS and LTS, respectively). These same measures were made in a comparative group of controls that had ACL surgery without secondary rupture and seen at mean 33.4 (range 28–37) months follow-up.Results
Mean MTS was significantly lower in the recurrent rupture group (10.5 ± 3.3° vs. 12.8 ± 2.7°; P = 0.02). This led to asymmetrical tibial slope with a mean LTS in the recurrent rupture group greater than the mean MTS (delta = –0.52 vs. +0.83). The mean LTS was not significantly different between groups (11 ± 3.4° vs. 12 ± 3.4°; P = 0.30).Conclusion
This study demonstrated that asymmetrical tibial slope due to a MTS lower than the LTS can be a risk factor for recurrent rupture of the ACL.Case-control
Level III. 相似文献3.
Background
There is a need of comprehensive work dealing with the quality of plasma for fractionation with respect to the IgG content as today most plasma derivates are used to treat patients with immunodeficiencies and autoimmune disorders. Therefore, a prospective study was carried out to analyse IgG levels before plasmapheresis and every 200 ml collected plasma.Materials and methods
Fifty-four experienced plasmapheresis donors were recruited for subsequent 850 ml plasmapheresis using the Aurora Plasmapheresis System. Donor? peripheral blood counts were analysed before and after plasmapheresis using an electronic counter. Total protein, IgG and citrate were measured turbidometrically before, during and after apheresis as well as in the plasma product. Furthermore, platelets, red and white blood cells were analysed as parameters of product quality.Results
An average of 2751 ± 247 ml blood was processed in 47 ± 6 min. The collected plasma volume was 850 ± 1 mL and citrate consumption was 177 ± 15 mL. A continuous drop of donors’ IgG level was observed during plasmapheresis. The drop was 13% of the IgG baseline value at 800 mL collected plasma. Total protein, IgG and cell counts of the plasma product met current guidelines of plasma for fractionation.Conclusion
Donors’ IgG levels during apheresis showed a steady decrease without compromising the quality of plasma product. 相似文献4.
Background & objectives
A previous review of transfusion practices in our institution between 1998 and 2008 showed a trend of high ratios of red cells (RC) to plasma (FFP) and platelets to RC towards the later years of review period. The aim of the study was to further evaluate transfusion practices in the form of blood product usage and outcomes following massive transfusion (MT)Methods
All adult patients with critical bleeding who received a MT (defined as ≥10 units of RC in 24 h) in 2008 and between January 2010 and December 2014 were identified. Blood and blood products transfused, in-hospital mortality, 24 h and 90-day mortality were analysed for the period 2010–2014. Blood and blood product usage, massive transfusion protocol (MTP) activation and use of ROTEM between 2008 and 2014 were compared.Results
A total of 190 MT including surgical (52.1%), gastro-intestinal bleeding (25.3%), trauma (11.6%) and obstetric haemorrhage (5.8%) episodes were identified between 2010 and 2014. The overall in-hospital mortality was 26.7% with a significant difference in 24 h (p = 0.04) and 90-day mortality (p = 0.02) between diagnostic groups. Comparing 2008 (n = 33) and 2014 (n = 23), there was no significant difference in median RC, FFP and platelet units, cryoprecipitate doses and RC:FFP ratio; however there was an increase in number of patients who used cryoprecipitate (54.5% vs 87%, p = 0.01).Conclusion
Aligned with haemostatic resuscitation, the trend continues in the form of increased use of plasma and higher RC:FFP transfusion ratios including an increase in number of patients receiving cryoprecipitate. 相似文献5.
Background
Rhnull is a rare autosomal recessive phenotype, which is characterized by the lack of Rh antigen expression on the red blood cells (RBCs). Rhnull of the regulator type is caused by RHAG mutation. In this study, a novel nonsense mutation in RHAG gene was identified in a Chinese Rhnull individual.Objectives and methods
Rh phenotypes of the Rhnull individual and his family members were typed by standard serological methods. DNA sequences of all ten exons of RHAG gene were analyzed using genomic DNA by polymerase chain reaction (PCR) and direct-sequencing.Results
Serological testing results showed a D-C-c-E-e- phenotype in the proband. Molecular analyses revealed a 540C > A mutation in exon 4 of RHAG gene was present at the homozygous state in the proband. His parents were heterozygous for the mutation, and his brother didn’t carry the mutation. The 540C > A mutation was nonsense mutation, which led to a premature stop codon (Tyr180stop).Conclusion
These results indicated that the 540C > A nonsense mutation in RHAG gene caused the regulator type of Rhnull phenotype in a Chinese individual. Our results contributed to a greater understanding of the genetic mechanisms of Rhnull phenotype. 相似文献6.
Hella Pfeiffer Susanne Achenbach Julian Strobel Robert Zimmermann Reinhold Eckstein Erwin F. Strasser 《Transfusion and apheresis science》2017,56(4):535-538
Background and objectives
Monocytes can be cultured into dendritic cells with addition of autologous plasma, which is highly prone to platelet contamination due to the apheresis process. Since platelets affect the maturation process of monocytes into dendritic cells and might even lead to a diminished harvest of dendritic cells, it is very important to reduce the platelet contamination. A new collection device (Spectra Optia) was analyzed, compared to two established devices (COM.TEC, Cobe Spectra) and evaluated regarding the potential generation of source plasma.Materials and methods
Concurrent plasma collected during leukapheresis was analyzed for residual cell contamination in a prospective study with the new Spectra Optia apheresis device (n = 24) and was compared with COM.TEC and Cobe Spectra data (retrospective analysis, n = 72). Donor pre-donation counts of platelets were analyzed for their predictive value of contaminating PLTs in plasma harvests.Results
The newest apheresis device showed the lowest residual platelet count of the collected concurrent plasma (median 3.50 × 109/l) independent of pre-donation counts. The other two devices and sets had a higher platelet contamination. The contamination of the plasma with leukocytes was very low (only 2.0% were higher than 0.5 × 109/l).Conclusions
This study showed a significant reduction of platelet contamination of the concurrent plasma collected with the new Spectra Optia device. This plasma product with low residual platelets and leukocytes might also be used as plasma for fractionation. 相似文献7.
8.
Roman Małachowski Olga Grzybowska-Izydorczyk Nelly Besson Anna Szmigielska-Kapłon Mateusz Nowicki Agnieszka Wierzbowska 《Transfusion and apheresis science》2017,56(4):563-565
Introduction
Thrombocytapheresis is an alternative treatment beneficial in rare circumstances, when cytoreductive agents are contraindicated, drug therapy gave no response or the expected response would be too slow. Here we present a case of a pregnant woman who underwent 5 thrombocytaphereses using Spectra Optia device to reduce circulating platelets (PLT) count and prepare for Cesarian section.Patient characteristics and performed treatment
A 39-year-old woman with diagnosed chronic myeloid leukemia (CML) was treated with interferon because of too high PLT count. The treatment was well tolerated but the effect was not satisfactory (PLT count remained high). Because of high risk of bleeding during childbirth, the healthcare providers decided to perform thrombocytapheresis to reduce circulating PLT count below 1000 × 10E3/μl, and to prepare the patient for a planned Cesarean section. The results are presented as mean ± SD.Results
Five therapeutic aphereses procedures were performed, with a Spectra Optia device (TerumoBCT). A mean of 1.3 ± 0.3 total blood volume was processed and we observed a mean PLT drop of 42.3 ± 17.7%. Each apheresis procedure resulted in a PLT level ≤1000 × 10E3/μl. PLT CE1 was high 50.6 ± 2.6% and reproducible. The white blood cell (WBC) loss was low (18.5% ± 11.0%). No adverse effects were observed.Conclusion
Therapeutic platelet depletion using the Spectra Optia? Apheresis System can be effective and safe during pregnancy. Thrombocytapheresis procedures were reproducible and Spectra Optia system successfully adjusted settings to each procedure conditions. Thrombocytapheresis seems to be a viable and safe option even in pregnant women. 相似文献9.
Akram Mansouri Mohammad Reza Adhami Mojarad Gholamreza Badfar Lila Abasian Shoboo Rahmati Wesam Kooti Mohammad Hossein YektaKooshali Ali Soleymani Milad Azami 《Transfusion and apheresis science》2017,56(3):404-409
Introduction
Asymptomatic Toxoplasma gondii transmitted from blood donors to receiving patients has become a concern. Numerous articles have been published on the epidemiology of T. gondii in blood donors in Iran and a systematic review of the results shows quite different findings, hence this study was conducted to determine the seroprevalence of T. gondii in Iranian blood donors using meta-analysis.Methods
We searched for epidemiological studies that were published until July 2016 in eight English databases including: Scopus, PubMed, Ovid, Science Direct, Cochrane, Web of Science, Springer, Wiley Online Library, and four Iranian databases: Magiran, Iranmedex, SID and Medlib as well as Google Scholar. To estimate pooled estimator, regarding high heterogeneity and significance of I2 index, Random effects model was used. Data analysis was conducted using Review Manager Ver5.3.5 and P < 0.05 was considered significant.Results
Among 10 studies with a sample size of 4538 Iranian blood donors, the seroprevalence of T. gondii was calculated to be 34.4% (CI95%: 24.9–43.9). The rate in males and females was 33.36% (CI95%: 19.92–46.81) and 37.63% (CI95%: 20.81–54.45), respectively. The seroprevalence of IgM and IgG antibodies among blood donors were 2.74% (CI95%: 0.55–4.92) and 31.84% (CI95%: 20.61–43.08), respectively. The seroprevalence of T. gondii was significantly associated regarding blood group (P = 0.04), but this relation was not significant with blood transfusion (P = 0.62).Conclusion
The results of this review indicate a high seroprevalence of T. gondii (about one-third) in Iranian blood donors and emphasizes the importance of screening the seroprevalence of T. gondii of donated blood in Iranian Blood Transfusion Organization. 相似文献10.
A. Radafy F. Marty A. Fantou A. Savigny Y. Bohu S. Blanchard J.-F. Chermann 《Journal de Traumatologie du Sport》2017,34(1):44-61
Introduction
The concussion, mild head injury, is found in contact sports event and whose evolution is marked by the appearance of post-concussion syndrome. A protocol standardized for concussed players was set up with a systematic review between 48 h and 72 h done to evaluate the severity of the concussion and consider the return to competition. During this consultation, we establish the score SCAT2 “Sport Concussion Assessment Tool 2”. The objective is to show or not a correlation between the SCAT2 score and duration of post-concussion syndrome.Methods
In this prospective study, patients were seen at 48–72 h after the concussion to establish the SCAT2 and followed until disappearance of post-concussion syndrome and return to play. Then, we used a correlation test to compare the score SCAT2 and duration of post-concussion syndrome. Then, we compared each item of SCAT2 score and the duration of post-concussion syndrome.Results
Overall, 174 players were included. On our population, there is a significant correlation between the SCAT2 and duration of post-concussion syndrome (R2 = 0.22 ; P = 1.10?10). Only, the number of symptoms present at 48 h appears predictive of prolonged post-concussion syndrome (R2 = 0.22 ; P = 7.10?11). No data appears significant for other items.Conclusion
On our population, SCAT2 score 48–72 h post-concussion is predictive of the duration of post-concussion syndrome, although this correlation appears moderate here (R2 = 0.22). 相似文献11.
Background and Objectives
Although several studies have reported on the use of children as donors for peripheral blood stem cells (PBSC), data on the predictive factors of CD34+ stem cell yield in healthy pediatric donors are very limited.Design and Method
We retrospectively analyzed factors predicting the yield for a target CD34 cell dose of >3 × 106/kg recipient body weight in 140 apheresis in 100 healthy pediatric donors. The donors were evaluated in four groups assigned according to their ages of being 0–4 years, 5–9 years, 10–14 years and 15–18 years. 38 donors underwent second apheresis, two of which required third apheresis.Results
Median age and body weight were 9.8 years (range: 2–18 years) and 35.8 kg (range 11–84 kg), respectively. The median number of CD34+ cells in first apheresis was 3.9 × 106/kg of recipient body weight (RBW) (range: 0.03–33 × 106/kg RBW). Sixty–two out of 100 donors (62%) needed only one apheresis. The CD34+ cell count in stem cell product and the amount of CD34+ cell yield considering donors’ body weight obtained from the first apheresis was not statistically different among defined age groups. On multivariate analysis, variables that had a significant impact on CD34+ cell collection being more than 3 × 106 kg RBW were donor to recipient weight ratio and periperal CD34+ cell count.Conclusion
Our data suggest that CD34+ stem cell yield can be predicted from circulating CD34+ cell concentration on apheresis day and donor to recipient weight ratio in healthy pediatric donors. 相似文献12.
Background
Proper stem cell mobilization is one of the most important steps in hematopoietic stem cell transplantation (HSCT). The aim of this paper is to share our 6 years’ experience and provide practical clinical approaches particularly for stem cell mobilization and collection within the series of more than 200 successive allogeneic HSCT at our transplant center.Subjects & Methods
Two hundred and seven consecutive patients who underwent allogeneic peripheral blood stem cell transplantation were included in this study. Age, sex, weight, complete blood counts, CD34+ cell counts, total collected amount of CD34+ cells, CD34+ cells per 10 l processed, mobilization failure and adverse events were reviewed.Results
Median age was 40.2 ± 12.9 (21–68) years and 46.4 ± 13.4 (17–67) years for donors and patients, respectively. The number of donors who had undergone adequate CD34+ cell harvesting and completed the procedure on the fourth day was 67 (32.8% of all patients). Only 12 patients required cell apheresis both on day 5 and 6. Apheresis was completed on day 4 and/or day 5 in 94.2% of all our donors. There was no significant association between CD34+ stem cell volume and age, gender and weight values of donors. Mobilization failure was not seen in our series.Conclusions
G-CSF is highly effective in 1/3 of the donors on the 4th day in order to collect enough number of stem cells. We propose that peripheral stem cell collection might start on day 4th of G-CSF treatment for avoiding G-CSF related side effects and complications. 相似文献13.
Saeed Mohammadi Shirzad Nasiri Mohammad Hossein Mohammadi Ashraf Malek Mohammadi Mohsen Nikbakht Mahdi Zahed Panah Hiva Safar Shayan Mostafaei Amir Hossein Norooznezhad Ahmad Reza Soroosh Kamran Alimoghaddam Ardeshir Ghavamzadeh 《Transfusion and apheresis science》2017,56(2):226-232
Objectives
One of the most important surgical issues applied in the treatment of pilonidal sinus disease is wound healing. The aim of this study was to investigate the possible effect of platelet-rich plasma (PRP) gel on accelerating wound healing in these patients.Methods
In this randomized, controlled, parallel group clinical trial, 110 patients were randomly allocated into two parallel groups with the same size (controls and treatment arm) after meeting inclusion and exclusion criteria. After the surgery, controls were treated by classic wound dressing while the case group was treated with PRP gel in a classic wound dressing platform. The patients were then evaluated for duration of antibiotics consumption, experienced pain and the time of returning to routine activities. Also, both groups were assessed for angiogenesis (by detecting CD34+ cells using immunohistochemical assay) and collagen sedimentation (masson’s trichrome staining) using pre-complete healing wound biopsy. All the statistical analyses were performed using SPPS 20 and p-values of less than 0.05 considered statically significant.Results
According to the results, patients treated with PRP gel went through a significantly faster healing process (8.69 ± 1.18 in controls and 4.78 ± 0.87 weeks in PRP gel treated ones with the P-value = 0.03) and returned to their routine activities (3.3 ± 0.64 for the treatment of arm and 6.5 ± 1.03 weeks for controls with the P-value = 0.00) while experiencing less pain (P-value = 0.00) and shorter anti-biotic consumption duration (P-value = 0.00).Conclusion
Considering the results, authors of this study suggest PRP gel treatment for post operation wound dressing of pilonidal sinus disease with healing by secondary intention. 相似文献14.
Matthew S. Karafin Erica Carpenter Amy Pan Pippa Simpson Joshua J. Field 《Transfusion and apheresis science》2017,56(3):345-351
Background
In adults with sickle cell disease (SCD), the effects of the red cell storage lesion are not well defined. The objectives of this study were to: (1) describe the distribution of storage ages provided to adults with SCD, and (2) evaluate clinical outcomes associated with storage age.Patients and methods
We performed a retrospective cohort study of adults with SCD managed with prophylactic simple transfusion regimens. Units were universally pre-storage leukocyte reduced and CEK-matched. Age of the unit was 42 days minus the difference between the expiration and transfusion dates. A mixed effects model, which accounts for a subject's contribution to repeated transfusion encounters, was used to investigate the association between storage age and the incidence of hospital encounters for infection and pain crises prior to the next red cell transfusion.Results
Over the study interval, twenty-eight steady-state adults with SCD received 627 units via simple transfusion over 281 outpatient encounters. Overall median unit storage age was 22 days (range: 2–42 days). Receipt of older units was associated with an increased incidence of emergency department or hospital admission for infection prior to the next transfusion (p = 0.04). There was no association between unit storage age and admission for pain (p = 0.4).Discussion
In a cohort of chronically transfused adults with SCD, we provide evidence that receipt of older units is associated with a higher rate of admission for infection. Prospective studies will need to validate these data and explore potential mechanisms by which these older units promote infection. 相似文献15.
Hyun-Ji Lee Kyung-Hwa Shin Duyeal Song Sun-Min Lee Chulhun L. Chang Chong Woo Chu Je Ho Ryu Tae Bum Lee Yong-Mok Park Kwang Ho Yang Byung Hyun Choi Hyung-Hoi Kim 《Transfusion and apheresis science》2017,56(3):385-388
Introduction
Therapeutic plasma exchange (TPE) is used for temporary support of liver function in patients presenting with early graft dysfunction after liver transplantation (LT) or liver surgery. We analyzed the effect of therapeutic apheresis on patients with liver disease.Methods
Between January 2011 and August 2016, 93 apheresis procedures were performed for 26 patients at our institution. Anti-ABO isoagglutination immunoglobulin (Ig) M titer was checked using a type A and type B 3% red blood cell (RBC) suspension in saline with two-fold serial dilutions of patient serum. Anti-ABO isoagglutination IgG titer was checked by a type A and B 0.8% RBC suspension using a low-ionic strength/Coombs card.Results
ABO-incompatible (ABOi) LT was the most common (n = 10, 38.5%) indication for apheresis; early graft dysfunction after LT (n = 8, 30.7%) was the second most common. Median initial IgM and IgG anti-ABO titers for ABOi LT recipients were 1:16 (range, 1:8–1:128) and 1:48 (range, 1:8–1:2048). We performed preoperative TPE in 10 recipients (median number of sessions, 1.5; range, 1–11). Among patients with early graft dysfunction, those who underwent living donor LT had better survival (4/4; 100%) than those who underwent nonliving donor LT (0/3; 0%). Patients who underwent living donor LT first and then additional LT also survived after three TPE sessions.Conclusion
Therapeutic apheresis is associated with a good survival rate and is essential for liver support in patients with early graft dysfunction after LT or posthepatectomy liver failure and during preparation for ABOi LT. 相似文献16.
Shin-ichiro Fujiwara Shuichi Kino Asashi Tanaka Yuichi Hasegawa Akihiko Yokohama Keizo Fujino Makino Shigeyoshi Mayumi Matsumoto Akihiro Takeshita Kazuo Muroi 《Transfusion and apheresis science》2017,56(5):708-712
Background
Premedication before transfusion is commonly administered in clinical practice despite a lack of evidence for its efficacy. The aim of this study was to clarify the status of premedication and evaluate expert opinions regarding its use in Japanese medical institutions.Method
Between May and July 2016, we conducted a questionnaire survey on premedication before transfusion in 252 medical institutes that were certified by an academic society or employed transfusion experts.Results
A total of 141 institutes (54.2%) responded, and hematologists (n = 113) comprised the most frequent respondents. The purpose of premedication was to prevent urticaria, pruritus, and fever, and washed blood products were used for anaphylactic shock or refractory transfusion reactions before. Drugs for premedication were intravenously administered either just before or 30 min before transfusion. Both inpatients and outpatients were premedicated in a similar manner, and institutional guidelines were not established. More than half of the experts recognized premedication as efficient and necessary, and premedication for previous transfusion reactions was frequently implemented, particularly for platelet transfusion or in patients with hematological diseases. Some institutions administered one or more drugs for premedication from the first transfusion. Antihistamines and hydrocortisone were the most frequently used as premedication.Conclusion
Our study reports the current status of premedication for transfusion in Japan. Antihistamines and hydrocortisone were most commonly used for premedication despite a lack of evidence of their use. These findings may help clarify the indications for premedication and the use of washed blood products. 相似文献17.
Nicole Arlt Remo Rothe Thomas Juretzek Heidrun Peltroche Torsten Tonn Rainer Moog 《Transfusion and apheresis science》2017,56(3):461-465
Background
Relatively slow-growing bacteria like Propionibacterium acnes represent a challenge for quality control investigations in sterility release testing of blood components and advanced therapeutic medicinal products (ATMPs).Methods
A convenient validation with 7 matrices was performed using buffy coat, stem cells, islet cells, natural killer cells, red blood cells, platelets and plasma in the microbial detection system Bact/Alert®3D incubator. All matrix samples were spiked twofold with Propionibacterium acnes with approximately 50 colony forming units (CFUs) per bottle in iAST and iNST culture bottles for 14 days using a multishot bioball. Additionally, the stem cell preparations were also incubated in iFAplus and iFNplus culture bottles, which include neutralizing polymers.Results
The Bact/Alert®3D-System detected Propionibacterium acnes in anaerobic culture bottles in buffy coat [3.3 d (= positive signal day to detection as mean value)], red blood cells [3.2 d], platelets [3.3], plasma [3.7 d], natural killer cells [3.3 d] and islet cells [4.9 d], resp. No growth of Propionibacterium was found in autologous stem cells using iAST and iNST culture bottles. However, Propionibacterium was safely detected in the iFNplus culture bottle with polymers in the stem cell matrix. A successful validation of media was performed.Conclusions
Our study shows that Bact/Alert®3D-System safely detects the relatively slow-growing bacterium Propionibacterium acnes in different matrices in a practical way except stem cells. Using the iFNplus culture bottle for stem cell products positive signals were observed. 相似文献18.
Fergun Yilmaz Nur Soyer Ruchan Uslu Mahmut Tobu Burcak Karaca Guray Saydam Fahri Sahin Filiz Vural 《Transfusion and apheresis science》2017,56(6):809-813
Background
In the literature, risk factors for poor mobilization were tried to identify. However, most of the studies consisted heterogeneous group of patients including both hematologic and oncologic malignancies. In this study, we aimed to identify the risk factors for poor mobilization in adults with solid tumors.Methods
We enrolled 49(47 men, 2 women) adult patients with solid tumor who were mobilized between September 2007 and February 2017. All the mobilization procedures were performed with G-CSF(10 μg/kg/day) with chemotherapy. Mobilization insufficiency was defined as peripheral blood CD34 + stem cell number less than 10/μl and/or total collected CD34 + cells less than 2.5 × 10 6/kg.Results
The patients were divided into two groups, patients with successful mobilization at the first attempt(group 1, 36 patients,73.5%) and poor mobilizers (group 2, 13 patients 26.5%). Second and third mobilization attempt was needed in 11 and 2 patients, respectively. The median number of CD34 + cells collected was 7,08 × 106/kg(0,6–19) with a median 4(1–6) apheresis. There was no statistical difference between two groups in terms of patient’s and mobilization characteristics. Only number of CD 34 + stem cells collected was statistically different (median 9,07 × 106/kg CD34 + cells in group 1 versus 2,14 × 106/kg in group 2, p < 0.05). The only possible risk factor that we could define was presence of organ metastasis.Conclusions
Since several methods and new drugs are available for peripheral stem cell collecting, risk factors should be identified clearly in adult population with solid tumors. So multicenter studies should be constructed for resolving this problem. 相似文献19.
Introduction
The WAA Registry allows detailed registration of hemapheresis data. Our center registers results there as well. We summarize our results as compared to those of the WAA Registry.Materials and methods
Hemapheresis results are registered in the WAA Registry in Umea, Sweden. The patients’ identity is protected by coding. General data (age, gender, weight, procedure, technique used etc.) or special data (occurrence and type of adverse reactions, health condition, quality of life etc.) are completed in a pre-defined form.Results
In 2006-2016, we registered 7,927 hemaphereses in 956 patients in the WAA Registry; 40.4% in men and 59.6% in women aged 53 ± 15 years. There were mostly no significant differences in the individual interventions between our center and the WAA Registry; only the share of cascade filtrations/rheophereses is quite different (9 times higher in our center ? 18.2% of interventions as compared to 2.1% in the WAA Registry). The share of photophereses (32.1%) is relatively high ? due to cooperation with the bone marrow transplantations department.Discussion and conclusion
In regular quality assessment, one center usually does not have enough data and experience with some diseases or interventions; therefore, comparison with the WAA Registry results is valuable not only for the quality of interventions but also for side effect prevention. On the other hand, the advantage is that every center has its unique code and may work quite independently (quick and independent non-competitive assessments). Five-minute duration of registration is advantageous in a time-demanding work; moreover, the registration is free. 相似文献20.