鹿瓜多肽与中医推拿联合治疗腰椎间盘突出症的疗效观察

目的探讨鹿瓜多肽与中医推拿联合治疗腰椎间盘突出症的疗效。方法选择笔者所在医院骨科腰椎间盘突出症病人126例,随机分为治疗组63例与对照组63例,对照组运用中医推拿手法结合卧床休息治疗,治疗组在对照组的治疗基础上静滴鹿瓜多肽,比较两组患者的治疗效果。结果在126例腰椎间盘突出患者中,治疗组治愈率为44.4%,对照组治愈率为27.0%,差异有统计学意义(P<0.05);治疗组治愈+显效为95.3%,对照组治愈+显效为79.3%,差异有统计学意义(P<0.01)。结论鹿瓜多肽与中医推拿联合治疗腰椎间盘突出症疗效明显,值得推广。     

鹿瓜多肽促进骨折愈合的疗效观察

目的：研究鹿瓜多肽治疗骨折愈合的疗效。方法选择骨折损伤的部位及性质基本相同的骨折患者50例,随机分为两组;治疗组25例、对照组25例。对照组仅给予常规治疗,治疗组常规治疗基础上应用鹿瓜多肽注射液10mL加入250mL 0．9％氯化钠注射液中静脉滴注,每天1次,疗程为10 d。结果治疗组有效率92．00％,对照组有效率80．00％;两组比较有显著性差异（ P＜0．05）。结论鹿瓜多肽注射液促进骨折愈合的疗效是肯定的。     

鹿瓜多肽治疗颈型颈椎病的临床疗效观察

目的观察鹿瓜多肽注射液对颈型颈椎病患者疼痛指标的影响,并评价其临床疗效。方法选择本院骨科门诊颈型颈椎病患者70例,随机分为两组,在常规理疗及手法治疗的基础上,观察组予以鹿瓜多肽注射液静滴,对照组以丹参针静滴,两组均以7天为1个疗程,治疗前后均采用视觉疼痛模拟评分法(visual analogue scale,VAS)记录疼痛分值。结果治疗前两组患者VAS评分无明显差异,治疗后观察组VAS评分降低较显著(P<0.05)。结论鹿瓜多肽注射液可明显减轻颈型颈椎病患者的疼痛,临床疗效肯定。     

丹红注射液联用鹿瓜多肽后对四肢骨折愈合的疗效观察

目的:观察丹红注射液联用鹿瓜多肽治疗新鲜四肢骨折的临床疗效。方法:27例老年四肢新鲜骨折患者随机分为二组,对照组(13例)丹红注射液治疗,治疗组(14例)丹红注射液联用鹿瓜多肽,余常规治疗2组相同。结果:治疗组骨折临床愈合时间、有效率与对照组比较差异有统计学意义(P<0.05),且治疗组未见明显不良反应。结论:丹红注射液联用鹿瓜多肽治疗四肢新鲜骨折更能有效促进骨折的愈合。     

鹿瓜多肽注射液治疗慢性腰腿痛的临床观察

目的观察鹿瓜多肽注射液治疗慢性腰腿痛的临床疗效。方法40例慢性腰腿痛患者随机分为二组,每组各20例,对照组常规治疗,在对照组常规治疗基础上加用鹿瓜多肽注射液8mg,1次／d,10d为1个疗程,连用1-2个疗程。结果治疗组症状缓解率与对照组比较差异有统计学意义（P〈0．05）。结论鹿瓜多肽注射液治疗慢性腰腿痛有效,能够减轻慢性腰腿痛患者症状。     

鹿瓜多肽与甲钴胺联合治疗腰椎间盘突出症的临床观察

目的观察鹿瓜多肽与甲钴胺联合治疗腰椎间盘突出症的临床效果。方法选择腰椎间盘突出症患者64例,随机数字表法分为2组,对照组应用甲钴胺注射液0．5mg,1次／d,3次／周,肌肉注射,治疗约2个月后作为维持量每隔1～3个月可给予一次,0．5mg肌肉注射。治疗组在此基础上用5％葡萄糖注射液或生理盐水注射液250～500ml溶解稀释鹿瓜多肽注射液,16～24mg／d静脉注射,10—15d为1个疗程。观察2组视觉模拟评分（VAS）情况。结果治疗组34例,治疗前VAS评分0—3分4例,4—6分10例,7～10分20例。治疗后VAS0～3分21例,4～6分8例,7～10分5例;对照组30例,治疗前VAS评分为0—3分4例,4—6分9例,7～10分17例。治疗后VAS评分0—3分8例,4—6分6例,7—10分16例。治疗组的治疗效果要显著好于对照组,差异有统计学意义（P〈0．05）。2组随访1个月至2年均未发现在治疗后的基础上症状再次加重。复查CT或磁共振成像（MRI）突出椎间盘未见缩小,但神经根水肿消失。结论对腰椎间盘突出症患者采用鹿瓜多肽和肌肉注射甲钴胺联合治疗是较理想的方法。     

硬膜外注射脉络宁配合手法复位治疗腰椎间盘突出症术后复发70例

目的观察硬膜外注射脉络宁配合手法复位治疗腰椎间盘突出症术后复发的疗效。方法将140例腰椎间盘突出症术后复发患者随机均分为两组,分别采用硬膜外注射脉络宁配合手法复位治疗(治疗组)与常规按摩、理疗(对照组)。结果治疗组总有效率、愈显率分别为97.14%和71.43%,均明显高于对照组的77.14%和42.86%(P>0.05);理疗28 d后,两组疼痛视觉模拟评分(VAS)均较治疗前明显下降,治疗组下降更明显(P<0.01)。结论采用硬膜外注射脉络宁配合手法复位治疗腰椎间盘突出症术后复发,效果显著,值得推广。     

复方骨肽注射液联合弥可保治疗腰椎间盘突出症的疗效观察

目的观察复方骨肽注射液联合弥可保配合常规西药、牵引治疗腰椎间盘突出症的疗效。方法将182例患者随机分为观察组(92例)和对照组(90例),观察组予复方骨肽注射液联合弥可保配合常规西药、牵引治疗,对照组予以常规西药、牵引治疗。结果观察组临床有效率为92.4%,高于对照组的75.6%(P<0.05)。结论复方骨肽注射液联合弥可保治疗腰椎间盘突出症疗效满意,且安全、无明显不良反应,值得临床推广应用。     

针刺联合正骨手法治疗腰椎间盘突出症的疗效观察

目的观察针刺联合正骨手法治疗腰椎间盘突出症的疗效。方法选取我院骨伤科门诊病例70例,按随机数字表分为两组,即试验组(40例)和对照组(30例)。采用针刺法结合正骨手法对腰椎间盘突出症进行治疗。结果总有效率,试验组为97.5%,对照组为73.3%。结论针刺联合正骨手法治疗腰椎间盘突出症治愈率高,效果显著,复发率低。此外,疗程短,价格低廉,具有广泛的应用推广价值。     

血塞通注射液治疗腰椎间盘突出症66例疗效分析

目的探讨血塞通注射液治疗腰椎间盘突出症的临床疗效。方法病例126例,全部符合腰椎间盘突出症诊断标准。随机分为2组,对照组给予口服中药汤剂、牵引、推拿、理疗等治疗;治疗组在对照组基础上给予血塞通注射液。结果经1个疗程治疗后,治疗组总有效率为97.0%。结论血塞通注射液治疗腰椎间盘突出症有良好效果,值得推广应用。     

药品化妆品抽检信息化应用的研究与思考

结合药品化妆品抽样检验政策分析、文献回顾和工作机制研究,梳理了国家药品化妆品抽样检验工作中的信息化应用,针对信息化建设的现状提出相应思考与建议。药品化妆品抽样检验在法律制度体系、工作机制等方面整体保持一致,信息化应用可相互借鉴、协同发展。药品化妆品抽样检验信息化工具可实现抽检全过程管理,应进一步强化药品化妆品抽样检验信息化建设的统筹规划和顶层设计,提升服务抽检计划制定、指导抽检实施和协助核查处置,加强抽检数据信息共享方面的技术支撑作用。     

Evaluation of the content and stability of the constituents of mother tinctures and tinctures: the case of Crataegus oxyacantha L. and Hieracium pilosella L

As a part of our investigations on the content and stability of herbal drug preparations, we evaluated the content and stability of tinctures and mother tinctures of Hawthorn leaves and flowers and Hawkweed. Hawthorn preparations are mainly used by patients with cardiac diseases; Hawkweed is employed for the treatment of cellulitis and obesity due to its diuretic properties. Both tinctures (DER 1:5) and mother tinctures (DER 1:10) are herbal preparations reported in the European Pharmacopoeia. The first preparation is obtained using dried herbal drugs; the latter is a homoeopathic preparation obtained with fresh plant material, often used in substitution of tinctures. The aim of this work was to assess the qualitative and quantitative profile of the constituents of the investigated preparations and the chemical stability of their marker constituents from long-term testing using HPLC assays. Characteristic constituents of Hawthorn leaves and flowers are flavonoids such as vitexin-2'-O-rhamnoside and hyperoside and oligomeric procyanidins. Characteristic constituents of Hawkweed are caffeoyl-quinic acid derivatives, flavonoids and a coumarin:umbelliferone. Our investigation showed that Hawthorn mother tincture had a higher concentration of procyanidins with respect to the tincture but the stability of these constituents were very low in both preparations. Total flavonoidic content was 3.33 mg/ml, about 1.5 times more than the content of mother tincture and the shelf-life t(90) was about 7 months for both preparations. For Hawkweed preparations a content of caffeoyl-quinic acid derivatives (ca. 4 mg/ml) was found, but their stability was good only in the tincture. The concentrations of flavonoids and umbelliferone were two times as much in the tincture with respect to the mother tincture. Stability of these two classes of constituents was good for both preparations over a 9-month period.     

Timing of Implantation and Closure of the Palatal Shelf in New Zealand White and Japanese White Rabbits

Two specific developmental events, namely implantation and palatal shelf closure, are of specific interest because they define, respectively, the beginning and the end of the treatment period in embryo-fetal developmental toxicity studies for pharmaceutical products. Thus, a detailed evaluation of the timing of implantation and closure of the hard palate is necessary to assure use of the proper exposure window in developmental toxicity studies in rabbits, the nonrodent species most commonly evaluated in regulatory developmental toxicology studies. The purpose of this study was to determine the timeline for implantation and closure of the hard palate in the New Zealand White rabbit, and to determine if this timeline differed in the Japanese White rabbit.

To describe the timing of implantation, the uteri from does of the New Zealand White rabbit and the Japanese White rabbit were examined on gestation days (GDs) 5 through 8 for macroscopic evidence of implantation. To assess palatal shelf closure, fetuses were removed on GDs 17, 18, and 19 and fixed in Bouin's solution. The fetuses were then categorized into five stages of palatal shelf closure: open (Stage I); approach of the palatal shelves (Stage II); partial closure of the hard palate (Stage III); full closure of the hard palate (Stage IV); and full closure of the soft palate (Stage V).

In both the New Zealand White and Japanese White rabbit strains, implantation was initiated on GD 6.5 and was completed on GD 7. Partial closure of the palate began on GD 17.5, and by GD 19, closure of the hard palate was completed in all fetuses, and closure of the soft palate was completed in 75–96% of the fetuses.

The timing of implantation and palatal shelf closure were comparable between the New Zealand White rabbit and the Japanese White rabbit. Therefore, treatment beginning on GD 7 and continuing until GD 19 encompasses the period of major organogenesis and is considered appropriate for use in developmental toxicity studies using either of these two strains of rabbits.     

广州市337份糕点的理化检验结果分析

目的 了解广州市区市售糕点的卫生质量状况,为广州市开展食品卫生监督工作提供一项可靠依据。方法随机抽取广州区各国营和个体食品加工厂、销售商店和个体销售摊位点糕点样品,按照国家标准GB／T5009—2003《糕点卫生标准分析方法》的检验项目进行检验与评价。结果共抽检337份糕点,总合格率为96．1％。三类糕点的合格率以“其他糕点类”最低为84．2％。其中酸价合格率为96．4％,过氧化值合格率为99．7％,铅、砷合格率均为100．0％。结论糕点的合格率有了一定的提高,酸价、过氧化值的合格率稍偏低,应加强对这两项指标的监测。     

中国与欧盟药品抽查检验监管对比研究

目的： 对比分析我国药品抽查检验与欧盟CAPs抽查检验监管机制,为制定相关政策提供参考。方法： 检索我国药品抽查检验与欧盟CAPs抽查检验公开信息,汇总分析我国药品抽查检验与欧盟CAPs抽查检验监管机制,就抽查检验目的、组织管理、抽检计划、抽样管理、检验管理和抽查检验结果运用管理等进行对比和分析。结果与结论： 我国药品抽查检验与欧盟CAPs抽查检验整体合格率均较高,体现整体药品质量稳定可控;我国药品抽查检验涉及品种多且覆盖面更广,综合质量分析服务监管和助力企业质量提升效果显著;欧盟CAPs抽查检验中三种管理工具即品种遴选工具、凭单更换机制和信息沟通机制对我国药品监管具有借鉴意义,欧盟CAPs抽查检验工作方向可供我国药品抽查检验借鉴。     

钉棒系统固定并前路减压植骨融合治疗胸腰段脊柱骨折的临床研究

目的：研究钉棒系统固定并前路减压植骨融合治疗胸腰段脊柱骨折的临床疗效。方法：将本院2003年11月～2010年1月收治的71例胸腰段脊柱骨折患者遵照知情同意原则分为治疗组36例和对照组35例,对照组采用AF系统固定治疗,治疗组采用椎弓根钉棒系统钉棒系统固定并前路减压植骨融合治疗,比较分析两组的治疗情况。结果：观察组的优良率为97.2%,明显高于对照组的77.1%;两组的并发症发生率分别为2.8%、5.7%,组间差异无统计学意义（P〉0.05）。结论：钉棒系统固定并前路减压植骨融合治疗胸腰段脊柱骨折获得较佳的临床疗效且安全性好,是临床首选的治疗方案之一。     

广东某高校教职工血压状况调研

目的 调查高校教职工血压状况,分析其高血压病患病率、职业、年龄分布特点。方法 按照1999年WHOASH高血压病诊断标准,对广东某高校教职工2002年526人的健康检查结果进行分析。结果 本组高校教职工高血压总患病率为15.39％,男为10.76％,女为21.01％;其中单纯收缩期高血压(含亚组)占44.44％,高血压Ⅰ级(含亚组)占40.74％。高血压Ⅱ级(中度)占12.35％, 高血压Ⅲ级.(重度)占2.47％。职业分组中高血压患病率:离退人员组为41.7％,后勤服务组为11.11％,行政管理组为5.21％:教师教辅组为1.44％。年龄分组中高血压患病率表现为:男女两组均随年龄增大而患病率增高,40岁以下组患病率明显低于50岁以上组(P<0.01)高血压患者集中分布在50岁以上各年龄组中,女性各年龄组高血压患病率高于男性。结论 本组高校教职工高血压患病率低于其他文献,说明高血压病的”三级预防”措施能有效防治高血压病极其并发症的发生、发展。     

Preparation and culture of precision-cut organ slices from human and animal

1.?Human and animal precision-cut organ slices are being widely used to obtain drug metabolism and toxicity profiles in vitro. These data are then used to predict what might be seen in human patients. The accuracy of this prediction and extrapolation of the findings based on human or animal in vitro systems to the findings that occur in vivo is dependent on both the quality of the tissue itself and the quality of the in vitro system.

2.?The quality of human organs used in research is dependent on procurement methods, warm ischaemia time, preservation solutions, cold ischaemia time, and donor-specific factors. It is important to confirm that the organs being used are highly viable and fully functional before using them in scientific studies.

3.?The optimal preparation and incubation of organ slices is also essential in maintaining slice viability and function. It is important to prepare the slices in a cold preservation solution, to prepare the slices at a correct thickness, and to incubate the slices in a system where the slice rotates in out of the oxygen atmosphere and medium.

4.?Meeting the criteria outlined here will lead to successful organ slice cultures for investigating drug-induced mechanisms and organ-specific toxicity.     

Effect of exposure to fluoride and acetaminophen on oxidative/nitrosative status of liver and kidney in male and female rats

BackgroundThis study was undertaken to investigate, the effect of 6 weeks treatment with acetaminophen (AAP) and fluoride (F), administered either separately or together, on nitric oxide generation, lipid and protein peroxidation, total antioxidant status and level of reduced glutathione in the liver and kidney of male and female Wistar Han rats. Also, the influence of AAP on F excretion in urine was determined.MethodsThirty adult male and female rats were divided into five equal groups of six each: (I) controls drinking tap water; (II) controls drinking tap water and receiving 1 ml of tap water intragastrically; (III) animals receiving 12 mg F/L in drinking water; (IV) animals receiving 150 mg AAP/kg b.w./day; (V) animals receiving 12 mg F/L in drinking water and 150 mg AAP/kg b.w./day.ResultsF and AAP given separately and both together enhanced oxidative and nitrosative stress in investigated tissues. No gender differences were observed in oxidative/nitrosative stress parameters during treatment with F and/or AAP. Interestingly, the combined exposure to F and AAP resulted in an enhancement of oxidative/nitrosative stress in kidney of male and female rats compared to the group treated separately with F and AAP. No additive effect in the measured parameters in the liver during co-exposure to both xenobiotics was noticed.ConclusionsAs expected, the urinary F excretion increased in an exposure time-dependent manner in rats receiving F or a combination of F and AAP. The study also showed that AAP significantly decreased urinary F.     

HPLC法测定复方银翘氨酚维C片中对乙酰氨基酚的含量

目的:建立HPLC法测定复方银翘氨酚维C片对乙酰氨基酚的含量.方法:色谱柱:大连依利特Hypersil ODS,流动相为甲醇-水(20∶80),检测波长为249 nm.结果:线性范围为2.0～10.0 mg·L-1(R=0.999 7),回收率良好,RSD=0.96%.结论:本法简便、准确、重现性好,可适用该制剂的含量测定.