超敏C反应蛋白、心脏型脂肪酸结合蛋白、肌酸激酶同工酶、肌红蛋白及肌钙蛋白联合检测对急性心肌梗死的诊断价值

目的 探讨超敏C反应蛋白(hs-CRP)、心脏型脂肪酸结合蛋白(H-FABP)、肌酸激酶同工酶(CK-MB)、肌红蛋白(MYO)及肌钙蛋白I(cTnI)联合检测对急性心肌梗死(AMI)诊断价值.方法 选择临床诊断为AMI患者46例(AMI组)和健康体检正常者96例(对照组),检测两组hs-CRP、H-FABP、CK-MB、MYO、cTnI水平,并观察比较AMI组在入院2、4、6h时各项观察指标变化情况.结果 AMI组患者入院2h时血清中hs-CRP、H-FABP、CK-MB、MYO、cTnI等水平明显高于对照组(t=46.0,均P＜0.01),AMI组在入院4h时各项观察指标明显高于入院2h时(t=46.0,均P＜0.01),入院6h时明显高于4h时(t=46.0,均P＜0.01);且hs-CRP、H-FABP、CK-MB、MYO、cTnI联合检测时,对AMI诊断的灵敏度为98.9％、特异性100％、阳性预测值100％.结论 hs-CRP、H-FABP、CK-MB、MYO、cTnI联合检测对AMI的诊断有重要临床价值.     

心脏型脂肪酸结合蛋白、缺血修饰白蛋白联合检测在急性冠脉综合征早期诊断和危险分层中的临床价值

目的:探讨心脏型脂肪酸结合蛋白(H-FABP)、缺血修饰白蛋白(IMA)联合检测在急性冠脉综合征(ACS)早期诊断和危险分层中的临床价值。方法:97例ACS患者根据疾病类型分为UA组(n=52例)和AMI组(n=45例),按院内死亡风险分为低危组(n=21例)、中危组(n=39例)和高危组(n=37例),同期选择50例门诊健康体检者作为对照组。比较各组H-FABP、IMA、c Tn I、CK-MB阳性率及血清H-FABP、IMA表达水平。结果:UA组和AMI组H-FABP、IMA诊断阳性率均明显高于c Tn I、CK-MB(P<0.05),且AMI组H-FABP诊断阳性率均明显高于UA组(P<0.05),而UA组和AMI组IMA诊断阳性率比较无显著差异性(P>0.05);ACS患者血清H-FABP、IMA表达水平均高于对照组(P<0.05),且随着危险程度加重,血清H-FABP、IMA表达水平均明显升高(P<0.05)。结论:H-FABP、IMA联合检测在ACS早期诊断和危险分层中均具有较高的临床价值。     

应用cTnI、BNP及心肌酶谱联合检测诊断急性冠脉综合征中的临床意义分析

目的分析血浆心肌肌钙蛋白(c Tn I)、血清脑钠肽(BNP)及心肌酶谱联合检测诊断急性冠脉综合征中的临床意义。方法选择确诊为急性冠脉综合征(ACS)患者35例作为观察组,和经冠状动脉造影检查显示正常的其他疾病患者35例作为对照组,应用即时检验(POCT)法检测c Tn I、BNP、CK-MB、CK、AST、LDH浓度,比较分析两组差异以及和ACS之间的关系。结果观察组患者入院时c Tn I、BNP、CK-MB、CK、AST、LDH浓度显著高于对照组患者(P0.05);治疗10d后观察组患者c Tn I、BNP浓度仍显著高于对照组患者(P0.05),但CK-MB、CK、AST、LDH浓度和对照组相比无显著差异(P0.05);治疗10d后,观察组患者c Tn I、BNP浓度显著低于入院时的检测浓度(P0.05)。结论使用POCT法对c Tn I、BNP及心肌酶谱进行联合检测并对治疗前后变化进行分析有助于早期明确诊断ACS并对治疗效果进行评估。     

心肌肌钙蛋白I和肌红蛋白对急性心肌梗死的诊断价值及预后评价

目的　探讨血清心肌损伤标志物心肌肌钙蛋白 I(c Tn I)和肌红蛋白 (Mb)对急性心肌梗死 (AMI)的诊断价值及预后评价。方法　连续搜集了急性胸痛的患者 12 1例 ,其中 AMI患者 6 3例 ,非 AMI患者 5 8例 ,对上述病例进行血清 c Tn I、Mb和肌酸激酶同工酶 (CK- MB)测定 ,观察其变化规律 ,分组比较心脏事件发生率。结果　 AMI发病 6 h以内 ,Mb首先升高 ,敏感性达 87.1%。发病 18～ 2 4 h,c Tn I敏感性达 10 0 .0 % ,且于发病第 5日仍维持敏感性达 6 1.1%。入院即刻 Mb敏感性 92 .1% ,高于 c Tn I 5 5 .6 %和 CK- MB 5 7.1% ;Mb特异性82 .8% ,低于 c Tn I96 .7%。入院次日晨 c Tn I敏感性 96 .8% ,高于 Mb6 1.9%和 CK- MB77.8%。AMI患者入院即刻 c Tn I增高组与正常组心脏事件发生率分别为 2 2 .9%和 3.6 % (P<0 .0 5 ) ,校正基线特征等多变量因素后 ,c Tn I是 AMI患者发生心脏事件独立的危险性预测因子 ,OR值 1.171(P<0 .0 5 )。结论　 c Tn I联合 Mb是诊断AMI的良好指标 ,其灵敏性和特异性能得到最佳体现。c Tn I亦有助于中后期 AMI的诊断。c Tn I是 AMI患者发生心脏事件独立的危险性预测因子     

血浆微小RNA-499、心肌肌钙蛋白I及超敏C反应蛋白在诊断急性心肌梗死患者中的价值

目的探讨血浆微小RNA-499(mi RNA-499)、心肌肌钙蛋白I(c Tn I)和超敏C反应蛋白(hs-CRP)联合检测对急性心肌梗死(AMI)诊断的价值。方法选择我院2012年7月至2013年12月收治的50例急性心肌梗死患者作为观察组,同时选择我院同期进行体检的无心脑血管疾病史的50例健康体检者为对照组,分别检测两组受检者的mi RNA-499、Myo、c Tn I和hs-CRP含量。结果心肌梗死者mi RNA-499、c Tn I和hs-CRP等水平均明显高于健康体检者,且比较差异有统计学意义(P<0.05);mi RNA-499相对表达量于发病0² h开始升高,22 h开始升高,2⁴ h达到最高峰,44 h达到最高峰,4⁸ h开始持续回落,c Tn I和hs-CRP水平均在08 h开始持续回落,c Tn I和hs-CRP水平均在0² h开始升高,82 h开始升高,8¹2 h达到高峰,1212 h达到高峰,12¹6 h开始回落。mi RNA-499、c Tn I和hs-CRP等水平均于3 d后回到正常水平。结论 mi RNA-499、c Tn I和hs-CRP各有优缺点,三者联合应用能取长补短,是一种很有实用价值的检测方法,对早期诊断AMI具有较高应用价值,值得推广。     

脂肪酸结合蛋白在急性心肌梗死患者早期诊断中的应用研究

目的探讨脂肪酸结合蛋白在急性心肌梗死(AMI)患者早期诊断中的应用价值。方法选取AMI患者60例根据胸痛到就诊时间不同分为4h组及4～8h组。对全部患者于就诊时即刻抽血检测心肌肌钙蛋白I(cTnI),肌酸激酶同工酶(CK-MB)及心脂肪酸结合蛋白(H-FABP),计算3种检测指标对两组患者诊断的特异度。结果两组患者在4h组的cTnI、CK-MB与H-FABP特异度差异对比有统计学意义(P<0.05)。结论 H-FABP在AMI早期诊断中特异度优于cTnI、CK-MB。     

心肌梗死诊断中心肌三项的重要作用

目的探讨评价急性心肌梗死（AMI）患者早期诊断中应用血清肌钙蛋白T（c Tn T）、肌酸激酶同工酶（CK-MB）以及肌红蛋白（Mb）心肌三项检测的重要意义。方法随机选取医院收治的80例AMI患者为研究对象（AMI组）,同时选择选取同期住院的无冠心病患者40例（非AMI组）作为对照,分别测定2组患者在胸痛发作后1-4h、4-8h、8-12h以及〉12h的c Tn I、Mb、CK-MB水平,同时对比分析心肌三项单项检测结果与三项联合检测结果对AMI的特异度和敏感度。结果 AMI组在胸痛发作后不同时段测定的c Tn I、Mb、CK-MB水平明显高于非AMI组,差异有统计学意义（P〈0.05）,且心肌三项联合检测对AMI诊断的特异度、敏感度均优于三项单独检测结果,差异有统计学意义（P〈0.05）。结论心肌三项检测对于早期诊断具有较高的临床应用价值,且三项联合检测可有效提高早期AMI患者的诊断正确率,对于临床及时救治,减少病死率,提高预后均具有重要作用。     

心脏型脂肪酸结合蛋白在急性心肌梗死早期诊断中的优势

目的:研究心肌型脂肪酸结合蛋白(H-FABP)对急性心梗患者早期诊断中的优势,比较不同心脏标志物诊断AMI的价值。方法:选择2013年1月~2014年1月于某院心血管内科就诊,胸骨后疼痛发作12d内但未确诊为急性心梗的患者165例,采肘静脉血5ml。采用快速检测试剂盒(胶体金法)检测H-FABP,同时测定肌钙蛋白T(cTnT)和肌酸激酶同工酶MB(CK-MB)。采用SPSS17.0软件比较3种心脏标志物以及各种标志物间联合运用在诊断急性心肌梗死(AMI)的灵敏度、特异度。在AMI组内,按照胸痛发作的时间不同,分为2h组,2~4h组,4~6h组,6h组,分别对不同组H-FABP、cTnT及CK-MB阳性结果进行比较。结果:H-FABP对AMI的诊断灵敏度(85.0%)高于cTnT(61.25%)和CK-MB(53.75%)以及cTnT和CK-MB的联合应用(65.0%)。单一运用H-FABP与联合运用H-FABP和其他标志物诊断AMI的灵敏度无统计学差异。所有指标的特异度均无统计学意义(P0.05)。对于起病2h内的AMI患者,H-FABP的阳性率(66.67%)显著优于CK-MB(16.67%,P0.05)和cTnT(25%,P0.05);对于起病2~4h内的AMI患者,H-FABP的阳性率(81.82%)显著优于CK-MB(45.45%,P0.05)和cTnT(50%,P0.05));对于起病4~6h内的AMI患者,H-FABP的阳性率(100%)优于CK-MB(77.78%,P0.05)和cTnT(88.89%,P0.05);对于起病6h以上的AMI患者,H-FABP的阳性率与CK-MB和cTnT无明显统计学差异。将同一检测指标不同起病时间段的阳性率比较显示:H-FABP在2h(66.67%)与2~4h(81.82%)2个时段无显著差异,但低于4~6h段及6h段(100%,P0.05)。结论:H-FABP早期诊断急性心肌梗死(尤其是4h内)较CK-MB和cTnT具有更重要的价值。     

急性心肌梗塞患者五项指标联合检测的临床意义

目的:探讨急性心肌梗塞(AMI)患者N末端脑钠肽前体(NT-pro BNP)、C反应蛋白(CRP)、心肌肌钙蛋白I(c Tn I)、肌红蛋白(Myo)及肌酸激酶同工酶MB(CKMB)的水平及临床意义。方法:选取某院2013年5月~2013年12月60例心肌梗塞患者为观察组,选取同期健康体检人群60例为对照组,同时检测两组标本NT pro BNP、CRP、c Tn I、Myo、CKMB的测定值并进行比较;观察组发作后分别在3h、6h、12h、24h、48h检测五项指标测定值并进行比较。结果:观察组与对照组NT-pro BNP、CRP、c Tn I、Myo、CKMB测定值比较有显著差异(P0.05)。五项指标中Myo的敏感度最高,AMI发作后最早升高,c Tn I的特异性最强,且持续时间最长,与最低项指标比较有显著差异(P0.05)。结论:N末端脑钠肽前体(NT-pro BNP)、C反应蛋白(CRP)、心肌肌钙蛋白I(c Tn I)、肌红蛋白(Myo)及肌酸激酶同工酶MB(CKMB)联合检测有助于早期准确诊断急性心肌梗塞。     

冠心病患者血清超敏C反应蛋白、肌钙蛋白、血脂水平的变化及临床意义

目的研究冠心病患者血清超敏C反应蛋白、肌钙蛋白、血脂水平变化及临床意义。方法随机选取2014年1~12月我院收治的50例冠心病患者设定为观察组,其中25例为稳定型心绞痛(SAP)、15例为不稳定型心绞痛(UAP)、10例为急性心肌梗死(AMI);再选择同期门诊体检的50例健康人员设定为对照组,两组均检测血清超敏C反应蛋白(hs-CRP)、肌钙蛋白(c Tn I)及血脂指标三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)等水平变化。结果观察组SAP组患者血清hs-CRP明显高于对照组;UAP和AMI组患者血清hs-CRP、c Tn I、TG、TC、LDL-C等指标水平均比对照组高,观察组HDL-C明显低于对照组;SAP和UAP组患者血清hs-CRP、c Tn I、TG、TC、LDL-C等指标水平明显低于AMI组,HDL-C水平高于AMI组;SAP组患者血清hs-CRP、c Tn I明显低于UAP组,两组对比差异显著,具有统计学意义(P<0.05);血清hs-CRP与TG、TC、LDL-C等指标呈正相关,与HDL-C指标呈负相关,血清hs-CRP与c Tn I呈正相关(P<0.05)。结论冠心病患者检测血清超敏C反应蛋白(hs-CRP)、肌钙蛋白(c Tn I)及血脂水平,可以预测和治疗冠心病患者的疾病。     

Antagonism of the trypanocide,berenil, and the nematocide,morantel, by tubocurarine

Summary The tubocurarine antagonism, (pA₂ values) of acetylcholine, suxamethonium, morantel and berenil were calculated as 6.5, 6.3, 6.4 and 6.2 respectively with the toad rectus abdominis muscle. It is suggested that tubocurarine competitively inhibits the morantel and berenil induced contracture of the toad rectus abdominis muscle.     

CAR在乳鼠肠、肝、心、脑、肾、肺组织的表达

目的检测柯萨奇-腺病毒受体(CAR)在乳鼠肠、肝、心、脑、肾、肺组织中的表达变化,为研究CVB感染引发病毒性疾病与组织中CAR表达水平的关联性提供参考。方法采用荧光实时定量PCR和Western blot法测定正常BALB/c乳鼠肠、肝、心、脑、肾、肺组织中CAR受体在核酸水平和蛋白水平的相对表达量。结果定量PCR显示CAR在乳鼠脑、肺、肠组织中表达显著高于肝、肾、心组织,差异均有显著性(P<0.01)。Western blot检测发现与定量PCR结果基本一致。结论 CAR的表达存在组织特异分布的特点。     

Hypertension, the Kuna, and the epidemiology of flavanols

A low sodium diet has often been implicated in the protection of low blood pressure populations from hypertension, but several other dietary factors, including those as yet unidentified, may also be involved. The Kuna Indians of Panama are free of hypertension and cardiovascular disease, but this is changing with migration to urban areas. We compared the indigenous diet of Kuna Indians living on remote islands in Panama (Ailigandi), whose lifestyle is largely hunter-gatherer, with those who have moved to a suburb of Panama City (Vera Cruz). Between April and October 1999, members of a Kuna research team administered a 118-item food frequency questionnaire to133 adult Kuna from Ailigandi and 183 from Vera Cruz. Single 24-hour urine collections and nonfasting blood samples were obtained. The Kuna in Ailigandi reported consuming a 10-fold higher amount of cocoa-containing beverages, 4 times the amount of fish, and twice the amount of fruit as urban Kuna (P<0.05 by t test). Salt added was ample among those living in Ailigandi and Vera Cruz according to both self-report (7.1+/-1.1 and 4.6+/-0.3 tsp weekly) and urinary sodium levels (177+/-9 and 160+/-7 mEq Na/g creatinine), respectively. The low blood pressure of island-dwelling Kuna does not seem to be related to a low salt diet. Among dietary factors that varied among migrating Kuna, the notably higher intake of flavanol-rich cocoa is a potential candidate for further study.     

Effects of d-amphetamine,maprotiline, l-dopa,and haloperidol on the components of the predatory behavior of the ferret,Putorius furo l.

Ferret predation on rats was examined in an arena. One hour before the test one of the following drugs was administered: d-Amphetamine (0.8 and 1.4 mg/kg IM), maprotiline (10 and 40 mg/kg orally), l-dopa (30 and 60 mg/kg orally), or haloperidol (0.14 and 0.6 mg/kg IM). Provided that capture was successful, the sequence of the behavioral components was not changed by these drugs. With the exceptions of paw movements and rolling over, which were not affected by the drugs, the components of predatory behavior were influenced differently. This leads to the assumption that a drug affects different mechanisms which control behavior. It is assumed that dopamine is involved in the control of capture elicitation as well as in the control of pursuit and biting. Capture elicitation was inhibited by d-amphetamine and l-dopa, but not by maprotiline, and was even facilitated by haloperidol. The orientation of pursuit movements and biting was impaired by l-dopa and improved by haloperidol, whereas maprotiline did not influence these components.     

Bromocriptine,dihydroergotoxine, methysergide,d-LSD,CF 25-397, and 29-712: Effects on the metabolism of the biogenic amines in the brain of the rat

The effects of the ergolene derivatives bromocriptine, dihydroergotoxine, methysergide, d-LSD, CF 25-397, and 29-712 on the metabolism of the biogenic amines in the brain of the rat were investigated.All six ergolene derivatives were found to increase the concentration of 4-hydroxy-3-methoxyphenylethylene glycol sulphate in the brain stem, i.e., to increase the turnover of noradrenaline (NA). Since in brain homogenates the agents inhibited the binding of ³H-dihydroergocryptine to -adrenoceptors, but only weakly inhibited the binding of ³H-alprenolol to -adrenoceptors, it is suggested that the increased turnover of NA may be a consequence of a blockade of -adrenoceptors by ergolenes.All of the ergolenes increased the concentration of serotonin (5-HT) in the cortex, but only bromocriptine and 29-712 increased the concentration of 5-hydroxyindoleacetic acid (5-HIAA), the other compounds decreasing the concentration of this metabolite, i.e., inhibiting 5-HT turnover. Reserpine-induced PGO waves in the cat were inhibited by all six compounds, bromocriptine and 29-712 being the least active. Both of these findings suggest that the ergolenes possess serotonergic activity. The increase in the concentration of 5-HIAA after bromocriptine and 29-712 may be secondary to some action on other systems.The actions of the ergolenes on the metabolism of dopamine (DA) in the striatum are more complex. Bromocriptine, 29-712, and, to a much lesser extent, dihydroergotoxine reduced the concentration of 3,4-dihydroxyphenylacetic acid (DOPAC), i.e., they inhibited DA turnover. These findings are compatible with the proposed dopaminergic activity of the drugs. CF 25-397 caused a slight increase in the DOPAC concentration at high doses, and d-LSD and methysergide caused pronounced increases. At doses below 1 mg/kg i.p., d-LSD decreased the DOPAC concentration. This biphasic effect of d-LSD may be due to interaction with different types of DA receptors or may reflect some secondary action of the compound.The profiles of activity of the various ergolenes are discussed. Bromocriptine and 29-712, wich have similar profiles of activity, can be clearly differentiated from the other ergolenes. CF 25-397 seems to be a potent and, at low doses, specific serotonergic drug.     

Quantitative Characterization of the Interaction Space of the Mammalian Carbonic Anhydrase Isoforms I,II, VII,IX, XII,and XIV and their Inhibitors,Using the Proteochemometric Approach

The critical role of carbonic anhydrases in different physiological processes has put this protein family at the center of attention, challenging major diseases like glaucoma, neurological disorders such as epilepsy and Alzheimer's disease, obesity, and cancers. Many QSAR/QSPR (quantitative structure–activity/property relationship) researches have been carried out to design potent carbonic anhydrase inhibitors (CAIs); however, using inhibitors with no selectivity for different isoforms can lead to major side‐effects. Given that QSAR/QSPR methods are not capable of covering multiple targets in a unified model, we have applied the proteochemometric approach to model the interaction space that governs selective inhibition of different CA isoforms by some mono‐/dihydroxybenzoic acid esters. Internal and external validation methods showed that all models were reliable in terms of both validity and predictivity, whereas Y‐scrambling assessed the robustness of the models. To prove the applicability of our models, we showed how structural changes of a ligand can affect the selectivity. Our models provided interesting information that can be useful for designing inhibitors with selective behavior toward isoforms of carbonic anhydrases, aiding in their selective inhibition.