胃癌患者可溶性白细胞介素—2受体与肿瘤坏死因子—α水平？…

为观察可溶性白细胞介素」－２受体（ｓＩＬ－２Ｒ）和肿瘤坏死因子－α（ＴＮＦ－α）对胃癌的诊断及估计预后的价值，应用酶联免疫双抗夹心法对７８例胃癌患者及对照者血清检测上述两种成分，进行临床观察。结果发现：胃癌患者血清ｓＩＬ－２Ｒ和ＴＮＦ－α水平明显高于胃良性疾病和正常人（均Ｐ〈０．０１）；胃癌Ⅲ、Ⅳ期高于Ⅰ、Ⅱ期（均Ｐ〈０．０１）；手术切除后（根治性或姑息性）ｓＩＬ－２Ｒ和ＴＮＦ－α显著下降（均Ｐ〈     

胃癌患者可溶性白细胞介素-2受体与肿瘤坏死因子-α水平的临床观察

为观察可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）和肿瘤坏死因子－α（ＴＮＦ－α）对胃癌的诊断及估计预后的价值，应用酶联免疫双抗夹心法对７８例胃癌患者及对照者血清检测上述两种成分，进行临床观察。结果发现：胃癌患者血清ｓＩＬ－２Ｒ和ＴＮＦ－α水平明显高于胃良性疾病和正常人（均Ｐ＜０．０１）；胃癌Ⅲ、Ⅳ期高于Ⅰ、Ⅱ期（均Ｐ＜０．０１）；手术切除后（根治性或姑息性）ｓＩＬ－２Ｒ和ＴＮＦ－α显著下降（均Ｐ＜０．０１）；手术后复发或转移患者血清中ｓＩＬ－２Ｒ和ＴＮＦ－α含量升高（均Ｐ＜０．０１）；如果患者血清ｓＩＬ－２Ｒ含量高于１０００ｋＵ／Ｌ同时伴有ＴＮＦ－α持续高于１２０μｇ／Ｌ，预兆生存期短。提示监测胃癌患者血清ｓＩＬ－２Ｒ和ＴＮＦ－α水平对临床诊断、病情估计和预后有重要意义     

大肠癌患者血清sIL-2R和TNF检测的临床意义

目的研究大肠癌患者血清可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）和肿瘤坏死因子（ＴＮＦ）变化的临床意义．方法采用双抗体夹心ＥＬＩＳＡ法对７６例大肠癌患者手术前后血清ｓＩＬ－２Ｒ和ＴＮＦ水平进行检测，其中根治性手术４８例，姑息性手术２８例，以４０例献血员为正常对照组．结果大肠癌患者血清ｓＩＬ－２Ｒ（Ｕ／Ｌ）和ＴＮＦ（ｐｇ／Ｌ）分别为６０３６±９４３和９６２±９１，明显高于正常对照组的２０８３±７９６和２５１±４７（Ｐ＜００１），两者的增高与大肠癌Ｄｕｋｅ分期及手术方式不同密切相关．结论动态观察血清ｓＩＬ－２Ｒ和ＴＮＦ的变化，可作为大肠癌诊断、评价疗效及监测预后的参考指标．     

肝硬化患者血清肿瘤坏死因子－α含量变化及意义

应用放免法测定５１例肝硬化患者血清肿瘤坏死因子α（ＴＮＦ－α）含量。结果表明：肝硬化患者血清ＴＮＦ－α明显高于对照组（Ｐ＜０．０５），且与血清总胆红质（ＢＩＬ）及转氨酶（ＡＬＴ）呈正相关（Ｐ＜０．０１，Ｐ＜０．０５），按Ｃｈｉｌｄ肝功能分级，Ａ、Ｂ、Ｃ各级组血清ＴＮＦ－α含量依次递增，且各级组间有显著性差异（Ｐ＜０．０１）。肝硬化合并感染患者ＴＮＦ－α显著高于未感染者（Ｐ＜０．０１），且易发生肝─肾综合征（Ｐ＜０．０５）。     

幽门螺杆菌感染患者白细胞介素—8和肿瘤坏死因子—α的表达

为研究幽门螺杆菌（Ｈｐ）感染时血清及胃粘膜组织白细胞介素（ＩＬ）－８、肿瘤坏死因子（ＴＮＦ）－α水平及其病理作用，采用放射免疫法和酶联免疫吸附试验夹心法分别检测了Ｈｐ（－）～（＋＋＋）者血清和胃粘膜组织中的ＩＬ－８、ＴＮＦ－α，并观察胃粘膜组织炎症活动程度与Ｈｐ感染密度的关系。结果：（１）胃粘膜组织ＩＬ－８、ＴＮＦ－α水平高低依次为Ｈｐ感染（＋＋＋）＞（＋＋）＞（＋）＞对照组，４组间差异均有显著意义（Ｐ＜０．０５）。（２）Ｈｐ根除后血清ＩＬ－８水平无明显变化，ＴＮＦ－α水平则明显下降；胃粘膜组织ＩＬ－８、ＴＮＦ－α水平下降，与根除前相比有显著性差异（Ｐ＜０．０５）。（３）胃粘膜组织炎症活动程度与Ｈｐ感染密度相关（精确概率值为０．００２６，Ｐ＜０．０１）。结果提示：Ｈｐ感染时胃粘膜组织ＩＬ－８、ＴＮＦ－α水平明显高于对照组，损伤程度随Ｈｐ感染密度增加而加重     

重症细菌性感染体内炎症介质与血清钙变化的临床观察

观察１０例重症感染患者体内肿瘤坏死因子（ＴＮＦ－α）、白介素-２（ＩＬ-２）和血清钙的变化。结果显示,对照组ＴＮＦ－ａ６７．４±３５．４,ＩＬ-２６１０±２１０,血青钙２．０１±０．１５;病例组ＴＮＦ－ａ１７６．３±５８．４,ＩＬ-２８６６±２４０,血清钙１．８０±０．１８,与对照组相比较,Ｐ＜０．０５或Ｐ＜０．０１,差异有显著性。血清钙浓度降低与ＴＮＦ－ａ,ＩＬ-２浓度增加密切相关。结合文献探讨重症感染患者体内炎症介质和血清钙的关系及其临床意义。     

肾综合征出血热患者血清IL-6、尿液肿瘤坏死因子、IL-6、IL-8的检测

目的探讨细胞因子在肾综合征出血热（ＨＦＲＳ）发病中的作用。方法采用双抗体夹心ＥＬＩＳＡ法对４８例ＨＦＲＳ患者及２０例正常人血清白细胞介素（ＩＬ）－６、尿液肿瘤坏死因子（ＴＮＦ）、ＩＬ－６、ＩＬ－８进行动态检测。结果ＨＦＲＳ患者血清ＩＬ－６、尿液ＴＮＦ、ＩＬ－６、ＩＬ－８含量较对照组明显增高（Ｐ＜０．００１）；发热期已增高，低血压期继续增高，少尿期达峰值；其含量随病情加重而升高，各型间差异有显著性；血清ＩＬ－６与特异性抗体升高有明显关系，与血清β２－微球蛋白（β２－ＭＧ）、血尿素氮（ＢＵＮ）、血肌酐（Ｃｒ）均呈高度正相关；尿液ＩＬ－６与ＴＮＦ、ＩＬ－８呈显著正相关（ｒ＝０．５６２１，Ｐ＜０．００５；ｒ＝０．３８４５，Ｐ＜０．０１）。结论ＨＦＲＳ患者病程中ＴＮＦ、ＩＬ－６、ＩＬ－８均处于高活性状态，ＩＬ－６与体液免疫反应亢进所致的免疫病理损伤有关，ＩＬ－６、ＩＬ－８、ＴＮＦ参与肾脏的免疫损伤，可作为判定患者预后和转归的指标。     

抗氧化剂抑制缺血后再灌注损伤相关的心肌细胞凋亡

目的 探讨老年急性胰腺炎（ＡＰ）患者血清一氧化氮（ＮＯ）和肿瘤坏死因子α（ＴＮＦ－α）的变化及临床意义。方法 对３２例老年ＡＰ患者及２０例健康老年人血清中ＮＯ和ＴＮＦ－α水平进行检测。结果 老年ＡＰ患者血清中ＮＯ浓度明显低于正常对照组，且随病情加重降低更为明显（Ｐ〈０．０１）；而血清ＴＮＦ－α浓度明显高于正常对照组，且随病情加重升高更为明显（Ｐ〈０．０１），ＮＯ和ＴＮＦ－α呈显著负相关（Ｐ〈０．０     

乙肝后肝硬化患者血清IL—1β,TNF—α变化对骨代谢影响的临？…

为探讨细胞因子对乙肝后肝硬化骨代谢的影响，采用ＲＩＡ及ＥＬＩＳＡ分别测定３６例乙肝后肝硬化患者血清ＩＬ－１β、ＴＮＦ－α水平及骨密度测量，并与１５例健康者对照。结果表明，肝硬化患者血清ＩＬ－１β、ＴＮＦ－α水平较对照组显著升高（Ｐ〈０．００１，Ｐ〈０．００１），其中骨质疏松（ＯＰ）组较非骨质疏松（ＮＯＰ）组均升高明显（Ｐ〈０．００１，Ｐ〈０．０１），且两组间Ｃｈｉｌｄ－ＰｕｇｈＡ、Ｃ级同级分别比较     

肝癌患者血清可溶性肿瘤坏死因子受体I的表达及临床意义

用ＥＬＩＳＡ方法测定了３０例肝癌患者血清可溶性肿瘤坏死因子受体Ｉ（ｓＴＮＦＲ－Ｉ）水平。结果表明，肝癌患者血清ｓＴＮＦＲ－Ｉ水平明显高于正常人（ｐ〈０．０１），且血清ｓＴＮＦＲＩ水平与临床分期相关。治疗有效者，血清ｓＴＮＦＲ－Ｉ水平均明显下降（ｐ〈０．０１）。治疗后２￣８月，３例无癌生存者，血清ｓＴＮＦＲ－Ｉ水平降至正常，而７例局部未控或复发转移者，血清ｓＴＮＦＲ－Ｉ水平则进行性上升。提示，血清ｓ     

ERCP and MRCP--when and why

Since the introduction of endoscopic retrograde cholangiopancreatography (ERCP) in the 1970s, gastroenterologists have a wide spectrum of diagnostic and therapeutic options in the biliopancreatic ductal system at their disposal. With its arrival in the 1990s, magnetic resonance cholangiopancreatography (MRCP) developed as a potent diagnostic tool in biliopancreatic pathology. Currently, MRCP is widely replacing diagnostic ERCP and thereby avoiding complications related to endoscopic technique.We summarize evidence-based data and demonstrate indications and differential indications for MRCP and ERCP in pancreatic disease. Complications related to the procedures and possible medical prevention are discussed. The feasibility of interventional endoscopy in pancreatic disease is reported in detail. The role of gastroenterologists in performing MRCP is outlined on the basis of practical examples.     

Micronutrients and infection: interactions and implications with enteric and other infections and future priorities

Symposium presentations have focused on the elegant molecular science and the biologic mechanisms by which micronutrients play critical roles in cellular and humoral immune responses, cellular signaling and function, and even in the evolution of microbial virulence. The concluding session examined the practical issues of how best to evaluate the nutritionally at-risk host, especially in the areas of greatest need-an analytical model of nutrient-immune interactions, implications of nutritional modulation of the immune response for disease, and the implications for international research and child health. This overview illustrated how malnutrition may be a major consequence of early childhood diarrhea and enteric infections, as enteric infections may critically impair intestinal absorptive function with potential long-term consequences for growth and development. The potentially huge, largely undefined DALY (disability-adjusted life years) impact of early childhood diarrheal illnesses demonstrates the importance of quantifying the long-term functional impact of largely preventable nutritional and infectious diseases, especially in children in developing areas.