茶多酚抗牙菌斑作用机理的体外研究

目的研究茶多酚对牙菌斑的影响。方法茶多酚用乙醇，乙酸乙酯从绿茶中提取，并用FeCl3及薄层层析法鉴定，S．sobrinus6715（g）的游离型和结合型GTF分别经硫酸铵和尿素处理获得，Somogyi法测GTF酶活性，染色法测蛋白质含量，葱酮法测葡聚糖含量。通过红外光谱鉴定葡聚糖。结果茶多酚能抑制变链球茵的生长、GTF酶活性及酶产生的胞外多糖。结论茶多酚能有效控制菌斑。     

茶多酚含片抗牙菌斑作用的观察

目的　研究茶多酚含片抗牙菌斑的作用和价值。方法　将 15 0例自愿受试者随机分为A、B试验组和对照组 ,每组 5 0例。试验组给予茶多酚含片 ,每人每次含服 1片 ,3次 /d。A组连续服用 3周后改为给予安慰剂 ,B组连续服用茶多酚含片 6周。结果　 2周后试验组的牙菌斑指数明显低于对照组 (P <0 0 1)。试验A组 3周后停用茶多酚含片 ,连续 3周检查仍显效。结论　茶多酚含片具有明显的抗牙菌斑作用。     

茶多酚抗牙菌斑作用机理的体外研究

研究茶多酚对牙菌斑的影响。方法 茶多酚用乙醇，乙酸乙酯从绿茶中提取，并用ＦｅＣｌ３及薄层层析法鉴定，Ｓ．ｓｏｂｒｉｎｕｓ６７１５的游离型和结合型ＧＴＦ分别经硫酸铵和尿素处理获得，Ｓｏｍｏｇｙｉ法测ＧＴＦ酶活性，染色法测蛋白质含量，蒽酮法测茚矣糖含量。     

茶多酚含片抗牙菌斑作用观察试食报告

有许多研究证实,一定浓度的茶多酚可以抑制或减少牙菌斑的数量及在牙面上的粘附。康齿王含片每片含茶多酚≥３６ｍｇ,经试验证实,康齿王含片对口腔致龋菌、变形链球菌、边远链球菌有抑菌作用和抗附着作用,０．５～１．０％康齿王有明显抑制变形链球菌转移酶活性作用。...     

表面活性剂ZonylFSC对人牙菌斑的影响

目的：研究表面活性剂ＺｏｎｙｌＦＳＣ对人牙菌斑的控制作用。方法：选择８位志愿者的４８个牙进行短期含漱试验，以蒸馏水为阴性对照，洗必太为阳性对照，以菌斑指数值（ＰｌＩ）代表控制菌斑的结果。结果：用０．２％ＺｏｎｙｌＦＳＣ含漱后ＰｌＩ值明显低于阴性对照组（Ｐ＜０．０１），菌斑指数降低约２１％，用０．２％洗必太较用同浓度ＺｏｎｙｌＦＳＣ的ＰｌＩ值低（Ｐ＜０．０１）。结论：ＺｏｎｙｌＦＳＣ能有效地控制菌斑，其菌斑控制作用弱于洗必太。     

十二烷基硫酸钠抗牙菌斑作用的实验研究

十二烷基硫酸钠（ｓｏｄｉｕｍｄｏｅｃｙｌｓｕｌｆａｔｅ，ＳＤＳ）常作为去垢剂广泛地应用于牙膏之中。已证实ＳＤＳ与羟基磷灰石（ＨＡ）有高度亲合力，通过ＳＤＳ与ＨＡ的结合，干扰蛋白质对ＨＡ的吸附，从而影响牙面获得性膜的形成。为进一步了解ＳＤＳ的抑菌和抗菌斑作用，本研究利用口腔恒化器和扫描电镜及同位素标记液闪检测技术，对ＳＤＳ的抗菌斑作用进行了观察。结果显示，经ＳＤＳ处理后的牙釉质表面没有成熟的牙菌斑形成，仅有少数细菌散在附着，其放射性明显少于对照组。提示ＳＤＳ具有抗菌斑作用，是牙膏中的天然抗菌斑成分之一。     

右旋糖酐酶对牙菌斑作用的研究

牙菌斑是龋齿和牙周病重要的致病因素之一。因此为了预防和控制龋齿和牙周病,抑制牙菌斑的发生和发展是至关重要的。利用右旋糖酐酶抑制牙菌斑,近年来受到各国口腔医学研究的重视。国处已有这方面的一些报道。本研究利用中国科学院微生物研究所提供的右旋     

牙菌斑体外模型的研究

采用从人牙菌斑中分离到的,目前认为与龋病发生有关的细菌,利用细菌连续培养技术,形成人工菌斑模型。结果表明该方法模拟的菌斑在形态、结构上与自然牙菌斑有极大的相似性,二者镜下实难区分。可以认为,该模型系统完全可以作为牙菌斑—牙釉质界面的体外模型,为研究牙菌斑致病性提供一个新的前景。     

茶多酚对牙周主要病原菌的体外抗菌活性研究

目的　探讨茶多酚对牙周主要病原菌的抗菌活性。方法　采用琼脂稀释法药物敏感试验,测定茶多酚对牙周主要病原菌的最小抑菌浓度(MIC)。结果　茶多酚对99株牙周主要病原菌均有明显的抗菌作用,对牙龈卟啉单胞菌的MIC50 、MIC90 为2 5 0mg/L ,对中间普氏菌的MIC50 、MIC90 为5 0 0mg/L ,对产黑色素普氏菌、具核梭杆菌和生痰二氧化碳噬纤维菌的MIC50为5 0 0mg/L ,MIC90 为10 0 0mg/L。本实验中1×10 6CFU/ml与1×10 8CFU/ml细菌接种密度对茶多酚的MIC值无明显影响。结论　茶多酚对牙周主要病原菌具有抗菌活性。     

Plaque surface area after rinsing with a low-level fluoride-containing Darjeeling tea

Plaque surface area after rinsing with a tea, containing low levels of fluoride, was evaluated in 30 human volunteers with a single-blind, one-operator, three-period, three-treatment cross-over study. The mouthwashes employed were: (1) tea (Darjeeling first flush:< 0.2 ppm F), (2) Meridol®, (3) control (tap water:< 0.2 pprn F). During the experiment the participants refrained from oral hygiene procedures and rinsed their mouth three tiroes a day after each meal with 20 ml of the respective rinse. After 3 d standardized photographs were taken of the upper canines and premolars and the plaque areas were measured planimetrically. Statistical analysis showed a marked decrease in plaque surface area after rinsing with Meridol, whereas no difference in plaque surface area was observed after mouthrinsing with tea and tap water, respectively.     

复方茶多酚漱口液治疗少儿牙龈炎疗效研究

目的 :探讨复方茶多酚漱口液对少儿牙龈炎的治疗效果。方法 :用随机方法对 2 40例 6～ 16岁少儿牙龈炎患者进行临床研究。试验组 12 0例 ,使用复方茶多酚漱口液 ;对照一组 60例使用洗必泰漱口液 ;对照二组 60例使用茶多酚漱口液。结果 :复方茶多酚漱口液能有效治疗少儿牙龈炎 ,复方茶多酚治疗 6d后 ,有效率明显高于茶多酚组 ,与对照二组相比 ,有显著性差异 (χ2 =5 .49P <0 .0 5 )与对照一组相比 ,无显著差异 (χ2 =1.0 5 2P >0 .0 5 ) ,实验室研究结果显示复方茶多酚具有较好的抗菌作用。结论 :复方茶多酚液是一种中西医结合的新型漱口液 ,具有良好的应用前景。     

A pilot study of the role of green tea use on oral health

To cite this article:
Int J Dent Hygiene 9 , 2011; 110–116
DOI: 10.1111/j.1601‐5037.2009.00440.x
Awadalla HI, Ragab MH, Bassuoni MW, Fayed MT, Abbas MO. A pilot study of the role of green tea use on oral health. Abstract: Introduction: An increasing number of people all around the world are turning to the nature by using the natural herbal products in both prophylaxes and treatment of different diseases. Green tea with active chemical ingredients posses diverse pharmacological properties that include anti‐inflammatory, anticariogenic, antioxidant and antibacterial effects. Aims: To assess the possible protective properties of green tea on oral health. Methods: The researchers used the following measurements: Streptococcus mutans count in saliva and plaque, Salivary and plaque pH values, Gingival Bleeding Index (GBI). The above‐mentioned measurements were applied to a sample consists of 25 subjects before and after rinsing with green tea for 5 min (short‐term study). While, S. mutans count for saliva and plaque and GBI measurements, this experimental intervention study was carried out in the El‐Azhar University dental clinic. Results: The results of this study showed that there was a statistically significant difference among subjects pre‐ and post‐rinsing with 2% green tea for 5 min concerning S. mutans count in saliva and plaque, salivary and plaque pH values and GBI. Conclusion: This study supports the effectiveness of local application of green tea as antibacterial and anticariogenic material as it decreases the acidity of the saliva and plaque, so it is a cost‐effective caries prevention measures especially in developing countries.     

茶多酚、MTAD和次氯酸钠液抑制粪肠球菌及其生物膜的作用比较

目的:检测中药茶多酚、MTAD、52.5 g/L次氯酸钠抑制根管壁粪肠球菌生物膜的能力,并分析中药茶多酚作为根管冲洗液的可行性。方法:采用微孔板滴定法检测茶多酚,MTAD和52.5 g/L次氯酸钠液对粪肠球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),采用琼脂扩散实验检测3种抗菌剂对粪肠球菌的抑菌圈。通过建立粪肠球菌感染根管3周和6周的模型,分别用茶多酚、MTAD和52.5 g/L次氯酸钠液对粪肠球菌感染后的根管进行处理,然后对根管壁生物膜中的细菌生存情况进行定性定量分析,比较这3种根管冲洗液抗粪肠球菌生物膜的能力。结果:MIC、MBC和琼脂扩散实验显示:3组冲洗剂对粪肠球菌均有抑制作用。在粪肠球菌感染根管3周的生物膜中,MTAD和次氯酸钠液可以完全抑制粪肠球菌生物膜,茶多酚处理后虽仍有粪肠球菌生长,但明显低于生理盐水处理组的细菌生存数,差异有统计学意义(P<0.05)。在粪肠球菌感染根管6周的生物膜中,次氯酸钠液可以完全抑制细菌生长,茶多酚和MTAD作用后仍有活菌生长,但与对照组相比,细菌生存数均显示了8个log值的下降,差异有统计学意义(P<0.05)。结论:52.5 g/L次氯酸钠液具有很强的抑制根管内粪肠球菌生物膜作用,而茶多酚和MTAD也具有一定的抑制粪肠球菌生物膜的能力。     

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目的观察口泰和复方茶多酚含漱液在固定正畸治疗中对牙龈炎的防治效果。方法选取2005—2006年在遵义医学院附属口腔医院门诊进行固定正畸矫治的病例40例,随机分为两组(每组各20例),分别使用口泰和复方茶多酚含漱液,观察正畸治疗前及治疗后1,2,3个月时牙龈炎发生情况,对牙龈指数(GI)、菌斑指数(PLI)进行检测,并对两组情况进行比较。结果使用不同含漱液后两组之间的GI、PLI差异均有统计学意义(P<0.05或<0.01)。结论复方茶多酚含漱液对固定正畸患者牙龈炎症的预防效果优于口泰。     

In vivo effect of four antibacterial agents upon the acidogenicity of dental plaque

Abstract – The present study was carried out to compare the effect of chlorhexidine, iodine, alcohol and hydrogen peroxide on the acidogenicity of dental plaque in vivo . Plaque pH changes after application of sucrose were measured before and at various time intervals after the topical application of the agents. The MIC values of the different agents were assessed against plaque bacteria in vitro . The antimicrobial activity of chlorhexidine and iodine were similar whereas alcohol and hydrogen peroxide were less effective in the concentrations employed. The results showed that chlorhexidine 2%, iodine 2%, and alcohol 70% inhibited pH drops for a 24-h period after treatment whereas 3% hydrogen peroxide had no effect. Chlorhexidine 0.2% inhibited acid production to a greater extent than did alcohol 50% and iodine 0.2%. It is suggested that retention of chlorhexidine in plaque may explain the observed prolonged effect.     

In vitro and in vivo studies on salifluor/PVM/MA copolymer/NaF combination as an antiplaque agent

Abstract Salifluor (5-n-octanoyl-3′-trifluoromethyl-salicylanilide), a broad spectrum antimicrobial agent, was investigated for its ability to inhibit dental plaque formation. A combination of salifluor with PVM/MA copolymer and NaF was optimized for its antiplaque effect in mouthrinse and dentifrice formulations based on a series of both laboratory and clinical studies. It was found that salifluor, a highly hydrophobic compound, could not be adequately solubilized with the conventional amount of sodium lauryl sulfate (SLS), the most commonly used anionic surfactant in oral hygiene products. However, it was possible to prepare stable mouthrinse formulations using a mixed surfactant system containing both anionic and nonionic surfactants. The most suitable mixture was found to be a combination of SLS, Pluronic and Tauranol m a proportion of 1:1:1, This combination provided adequate stability and high antimicrobial activity as determined by in vitro microbiological tests. Addition of a PVM/MA copolymer to the formulation improved the adsorption and retention of salifluor on simulated tooth surfaces in vitro (saliva coated hydroxyapatite disks) by almost two-fold and also increased the antiplaque efficacy in both laboratory and human clinical studies, it was also found that a non fluoride dentifrice containing a combination of salifluor and PVM/MA copolymer with a dicalcium phosphate dihydrate abrasive, was highly effective in reducing smooth surface and fissure caries in rats. The results of the present studies demonstrated that salifluor is an effective antiplaque agent in mouthrinse and dentifrice when carefully formulated to maximize its delivery and bioavailability on oral surfaces. They also illustrated the difficulties encountered in exploiting the antimicrobial efficacy of highly hydrophobic, nonionic antimicrobial agents such as salifluor in commonly used oral hygiene vehicles.