龈沟液中TNFα的检测及其与IL—8的关系

目的：对牙周炎患者龈沟液（ＧＣＦ）中ＴＮＦα进行定量分析，初步探讨ＴＮＦα与牙周炎临床指标及ＧＣＦ中ＩＬ－８的关系。方法：采集２３例成人牙周炎患者ＧＣＦ，并记录临床指标：菌斑指数，牙龈指数，牙周袋深和附着水平。用Ｗｈａｔ－ｍａｎⅠ号滤纸条采集ＧＣＦ，通过ＥＬＩＳＡ法对ＧＣＦ中ＴＮＦα进行检测。结果：牙周炎ＧＣＦ中ＴＮＦα的检出率为４４．１２％，含量范围为０．００１～０．０２４ｍｇ／Ｌ，ＧＣＦ中ＴＮＦα含量与牙周附着水平，ＧＣＦ中ＩＬ－８水平相关显著；而健康组ＧＣＦ中ＴＮＦα检出率为０。结论：ＴＮＦα参与牙周炎症反应，并与细胞因子ＩＬ－８有一定的联系。     

牙周炎患者红细胞免疫状态的初步分析

目的：通过对牙周炎患者外周血红细胞Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）和红细胞免疫复合物花环率（ＲＢＣ－ＩＣＲ）的检测,观察青少年牙周炎（ＪＰ）、快速进展型牙周炎（ＲＰＰ）、成人牙周炎（ＡＰ）的红细胞免疫功能状况。方法：采用红细胞酵母菌花环试验方法。结果：ＪＰ、ＲＰＰ患者红细胞免疫粘附力明显低于正常人。ＪＰ、ＲＰＰ患者ＲＢＣ－Ｃ３ｂＲＲ分别为（１０．０３±０．９２）％、（１２．２０±０．９４）％,与对照组（１５．９０±１．７７）％有显著差异（Ｐ＜０．００１,Ｐ＜０．０１）;ＲＢＣ－ＩＣＲ分别为（２７．８９±１．７７）％、（２７．０１±１．７９）％,与对照组（３０．６４±３７）％有显著性差异（Ｐ＜０．０１）;ＡＰ患者ＲＢＣ－Ｃ３ｂＲＲ为（１６．０２±１．２５）％、ＲＢＣ－ＩＣＲ为（３０．０４±１．７３）％,与对照组无显著性差异（Ｐ＞０．０５）。结论：提示ＪＰ、ＲＰＰ的发病机理可能与宿主的红细胞免疫功能低下有关。     

牙周炎患者龈沟液中IL—1活性检测

目的：通过对牙周炎龈沟液（ｇｉｎｇｉｖａｌｃｒｅｖｉｃｕｌａｒｆｌｕｉｄ，ＧＣＦ）中ＩＬ－１活性进行检测，初步探讨ＩＬ－１在牙周炎症中的作用。方法：以成人牙周炎为研究对象，通过细胞检测法对患牙ＧＣＦ中ＩＬ－１活性进行检测。结果：在患牙ＧＣＦ中可测到具有较高活性的ＩＬ－１，而在健康ＧＣＦ中未能测到。结论：牙周炎时局部有活性ＩＬ－１渗出，ＩＬ－１是参与牙周炎症反应的重要细胞因子。     

牙周病患者治疗前后龈沟液中碱性磷酸酶水平的变化

选择牙周健康者（Ｈ）及边缘性龈炎（ＭＧ）、慢性成人牙周炎（ＣＡＰ），快速进展型牙周炎（ＲＰＰ）患者共３５人，２０４颗牙齿，测定其龈沟液（ＧＣＦ）中碱性磷酸酶（ＡＬＰ）的水平。发现牙周组织有破坏的患牙（ＣＡＰ和ＲＰＰ组），其龈沟液（ＧＣＦ）－碱性磷酸酶（ＡＬＰ）水平高于牙周无破坏者（Ｈ和ＭＧ组）ＧＣＦ－ＡＬＰ水平与牙周临床指标呈高度正相关，牙周治疗后临床指标及ＧＣＦ－ＡＬＰ水平均明显下降。提示ＧＣＦ     

龈沟液中的炎症调节因子——白细胞介素8

为研究白细胞介素８（ｉｎｔｅｒｌｅｕｋｉｎ－８，ＩＬ－８）在牙周炎中的作用，作者应用ＥＬＩＳＡ法检测了５４例（１０５个牙）成人牙周炎和２４名（５４个牙）健康对照者龈沟液中ＩＬ－８的水平。结果显示：龈沟液中ＩＬ－８的浓度低于３０μｇ／Ｌ时，ＩＬ－８与龈沟出血指数呈正相关（Ｐ＜０．０１）。龈沟液中ＩＬ－８的浓度高于３０μｇ／Ｌ时，ＩＬ－８与出血指数和探诊深度呈负相关（Ｐ值均小于０．０１）。９１％的牙周炎患牙龈沟液中ＩＬ－８低于３０μｇ／Ｌ。结果提示：ＩＬ－８可能具有抗炎和致炎的双重调节作用；其抗炎和致炎作用的分界值约为３０μｇ／Ｌ。高浓度的ＩＬ－８具有抗炎作用，低浓度的ＩＬ－８具有致炎作用。牙周炎时，龈沟液中的ＩＬ－８主要起致炎作用。     

龈沟液中天冬氨酸转氨酶及乳酸脱氢酶水平在牙周炎诊断中的作用

对３０例牙周炎患者的１２０颗牙及９例健康对照者的３６颗牙的龈沟液（Ｇｉｎｇｉｖａｌ Ｇｒｅｖｉｃｕｌａｒ ｆｌｕｉｄ，ＧＣＦ）中天冬氨酸转氨酶（Ａｓｐａｒｔａｔｅ ａｍｔｈｏｒａｎｓｆｅｒａｓｅ，ＡＳＴ）及乳酸脱氢酶（Ｌａｃｔａｔｅ ｄｅｈｙｄｒｏｇｅｎａｓｅ，ＬＤＨ）水平进行了测定，发现牙周炎患者ＧＣＦ－ＡＳＴ及ＧＣＦ－ＬＤＨ水平明显高于健康对照者。并且两种酶水平与牙周临床指标呈高度正相关。提示     

龈沟液中I型胶原自身抗体的检测

本研究用间接ＥＬＩＳＡ法检测成人牙周炎（ＡＰ）２７例为４４牙，快速进展性牙周炎（ＲＰＰ）１０例２６牙及吉健康对照（Ｈ）１２例牙龈沟液中的Ｉ型胶原自身抗体（ＩｇＧ）。结果ＡＰ，ＲＰＰ及Ｈ各组的Ｉ型胶原抗体检出率分别为２７．３％，２６．９％和２２．２％，各组间的检出率无统计学差异（Ｐ〉０．０５），且Ｉ型胶原抗体水平与临床参数也无显著相关。本研究结果不支持牙周炎时机体对Ｉ型胶原产生了自身体液免疫反应，牙     

TNF—α及IL—1β与多形核白细胞在牙周炎症组织中？…

目的 探讨快速进展性牙周炎（ｒａｐｉｄｌｙ ｐｒｏｇｒｅｓｓｉｖｅ ｐｅｒｉｏｄｏｎｔｉｔｉｓ，ＲＰＰ）患者局部牙周组织中多形核白细胞（ｐｏｌｙｍｏｒｐｈｏｎｕｃｌｅａｒ ｎｅｕｔｒｏｐｈｉｌ，ＰＭＮ）过度浸润及激活与肿瘤坏死因子α（ｔｕｍｏｒ ｎｅｃｒｏｓｉｓ ｆａｃｔｏｒ－α）和白细胞介素１β（ｉｎｔｅｒｌｅｕｋｉｎ－１β，ＩＬ－１β）的关系。方法 采用夹心ＥＬＩＳＡ法检测２２例ＲＰＰ患者共４     

牙周病患者牙龈组织前列腺素E_2、血栓素B_2、6－酮－前列腺素F_（1α）水平的研究

采用ＲＩＡ对１１例健康牙龈组织（Ｈ）、１１名龈炎（Ｇ）患者的１７个牙、２６名成人牙周炎（ＡＰ）患者３６个牙和１４名青少年牙周炎（ＪＰ）患者的２０个牙的牙龈组织同时测定ＰＧＥ_２、ＴＸＢ_２、６－Ｋ－ＰＧＦ_（１α）的含量。结果以ＰＧＥ_２最多，ＴＸＢ_２次之，６－Ｋ－ＰＧＦ_（１α）最少；３种前列腺素（ＰＧｓ）均随病变严重程度而增加，除Ｇ组ＴＸＢ_２和６－Ｋ－ＰＧＦ_（１α）与Ｈ组相差显著（Ｐ＜０．０５）外，其余各病变组与Ｈ组比，３种ＰＧｓ均相差非常显著（Ｐ＜０．０１），ＡＰ组和ＪＰ组比Ｇ组也有非常显著升高（Ｐ＜０．００１），而ＡＰ和ＪＰ组间相差不显著（Ｐ＞０．０５），表明３种ＰＧｓ在牙周病发病中具有重要作用。     

成人牙周炎患者治疗前后龈沟液中谷胱甘肽过氧化物酶水平的变化

目的：了解谷胱甘肽过氧化物酶（ＧＰｘ）在牙周病变过程中的变化及其临床意义。方法：选择２３例成人牙周炎（ＡＰ）患者的４４颗患牙,记录临床指标ＧＩ、ＰＤ、ＡＬ,并测定治疗前后ＧＣＦ－ＧＰｘ水平。结果：ＧＣＦ－ＧＰｘ与ＰＤ、ＡＬ负相关（Ｐ〈０．０５,Ｐ,０．００５）,与ＧＩ无明显相关性。牙周治疗后临床指标均明显下降（Ｐ〈０．００１）,ＧＣＦ－ＧＰｘ水平明显上升（Ｐ〈０．００１）,但ＧＣＦ－ＧＰＸ水平的上     

Determination of pseudocholinesterase activity in the gingival crevicular fluid, saliva, and serum from patients with juvenile periodontitis and rapidly progressive periodontitis

By use of a spectrophotometric method, pseudocholinesterase (PCE) activities were determined in gingival crevicular fluid (GCF), saliva, and serum from patients with juvenile periodontitis (JP) and rapidly progressive periodontitis (RPP) and from controls. The PCE activity in the GCF samples was 181 +/- 48 U/L in the JP group, 588 +/- 135 U/L in the RPP group, and 88.5 +/- 29.1 U/L in the control group. Saliva PCE activity levels were 9.1 +/- 1.7 U/L in the JP group, 21.8 +/- 4.5 U/L in the RPP group, and 12.7 +/- 0.8 U/L in the control group. GCF contained a higher PCE activity than saliva but a lower one than that of serum. The RPP group had a significantly higher PCE activity in both the GCF and saliva samples. No significant differences could be found regarding serum enzyme levels. Also, no significant correlations were present between biochemical values and the severity of periodontal disease. GCF may be an important source for the PCE content of saliva. It is suggested that the increased PCE activity seen in RPP patients might be caused by either the direct production of esterases by bacteria or the induction of esterases during periodontal destruction.     

Determination of serum antibody against Bacteroides gingivalis from rapidly progressive periodontitis and juvenile periodontitis patients

Bacteroides gingivitis (Bg) is one of the major pathogens associated with periodontitis. This is the first report in China on serum antibody level against Bg from patients with rapidly progressive periodontitis (RPP) and juvenile periodontitis (JP) using ELISA method. 21 RPP patients, 20 JP patients and 30 healthy subjects (H) were involved in this study. The results showed that the ratio of positive antibody response was 100% in RPP, 80% in JP and 30% in control group. The antibody response in both RPP and JP groups were significantly greater than that in healthy group (P less than 0.001). The difference between RPP and JP groups were also statistically significant (P less than 0.05).     

The role of IL-1 beta and TNF-alpha in hyper-reactivity of neutrophils in rapidly progressive periodontitis patients]

OBJECTIVE: Neutrophils (PMNs) from rapidly progressive periodontitis (RPP) was found to generate abnormally high levels of oxygen radicals. Elastase activity in gingival crevicular fluid (GCF) from RPP was also found much higher. It suggested that PMNs in some RPP patients are hyper-reactive. The purpose of this study was to investigate the mechanism of PMN hyper-reactivity by surveying the correlation of TNF-alpha level with elastase activity in GCF and by evaluating the association between PMN infiltration and the expression of IL-1 beta and TNF-alpha in gingival tissues from RPP patients. METHODS: 41 GCF samples from 22 RPP patients and 34 GCF samples from 11 healthy controls were collected. The total amount of TNF-alpha in GCF was detected using ELISA. The elastase activity was measured with a low molecular weight substrate (S2484) specific for granulocyte. The correlation of TNF-alpha level with elastase activity in a GCF sample was analyzed with Spearman correlation. 20 gingival specimens were obtained respectively from 10 RPP patients and 5 periodontally healthy controls. The expression of IL-1 beta and TNF-alpha was detected with immunohistochemistry. The distribution of PMN was observed with hematoxylin and eosin staining. RESULTS: Total amount of TNF-alpha in GCF was positively correlated with elastase activity (r = 0.44, P < 0.05). The IL-1 beta- and TNF-alpha-positive cells in gingiva were superimposed in areas where PMNs infiltration predominant. CONCLUSION: The hyper-reactivity of PMN in RPP patients was related to locally produced IL-1 beta and TNF-alpha.     

龈沟液中的IL—8抑制因子及其生物活性

In order to confirm the existence of Interleukin-8 (IL-8) inhibitor and its biological activity in gingival crevicular fluid (GCF), this study examined the GCF taken from 7 adult periodontitis (AP) patients. In neutralization test of IL-8, the results showed that the mean level of IL-8 was less than 1 ng/ml, which had been added into the GCF before ELESAs was performed to measure the amount of IL-8 in GCF. The mean level of IL-8 in the GCF of AP group was significantly lower than that of healthy group (P < 0.001). In biological activity test of IL-8 inhibitor, using pooled GCF taken from 8 AP patients (23 teeth), the results showed that the GCF (without recombinant human IL-8, rhIL-8) caused more white blood cell (WBC) migration than blank control group (physiological saline) did. When the amount of rhIL-8 increased in GCF from 0.1 microgram to 1 microgram, the WBC count increased by 18.6% which was less than the increase rate (49.1%) in control group with same dose of IL-8. In the saline group containing rhIL-8, the WBC chemotactic response appeared as an inverted "V"-shape curve. All these data suggested that 1. Certain kinds of IL-8 inhibitor exist in GCF which can "cleave" IL-8. 2. The level of IL-8 inhibitor(s) increases significantly in the GCF from periodontitis sites. 3. The GCF of adult periodontitis patient has strong chemotactic effects on WBC. IL-8 inhibitor(s) in GCF can slightly suppress the chemotactic effect induced by IL-8. When assessing the role of IL-8 in pathophysiology, the high and low dose of IL-8 might have different sense.     

龈沟液中存在白细胞介素8降解酶及其自身抗体

目的探究龈沟液中是否存在白细胞介素８（ｉｎｔｅｒｌｅｕｋｉｎ８,ＩＬ８）的抑制因子。方法（１）将１３例成人牙周炎的１４份及８例牙周健康者的９份龈沟液样本各自一分为二,１／２加入丝氨酸蛋白酶抑制剂──苯甲基磺酰氟,另１／２加入等量的磷酸盐缓冲液作为对照。ＥＬＩＳＡ法检测各样本中的ＩＬ８含量。（２）对１５例成人牙周炎的４１份龈沟液样本,用间接ＥＬＩＳＡ法测定龈沟液中的ＩＬ８自身抗体（ＩｇＧ）。结果（１）加入酶抑制剂的龈沟液中ＩＬ８的检出量（３．０１ｍｇ／Ｌ±５．７９ｍｇ／Ｌ）显著高于对照组（０．０５ｍｇ／Ｌ±０．１５ｍｇ／Ｌ）,Ｐ＜０．００１。（２）龈沟液中ＩＬ８自身抗体水平高于阴性对照标准差３倍。龈沟液中加入大肠杆菌超声粉碎液对此检测结果无明显影响。结论龈沟液中存在能降解ＩＬ８的丝氨酸蛋白酶;成人牙周炎的龈沟液中存在ＩＬ８自身抗体     

Relationship between interleukin-6 levels in gingival crevicular fluid and periodontal status in patients with rheumatoid arthritis and adult periodontitis

BACKGROUND: The aim of this study was to determine and compare interleukin-6 (IL-6) levels in gingival crevicular fluid (GCF) and clinical periodontal findings in patients with rheumatoid arthritis (RA) and adult periodontitis (AP). METHODS: A total of 45 patients divided into 3 groups (15 patients with RA and AP, 15 patients with AP, and 15 periodontally healthy subjects) were included in this study. Plaque index (PI), gingival index (GI), sulcus bleeding index (SBI), probing depth (PD), and attachment level (AL) values for each patient were recorded. Enzyme-linked immunosorbent assay for quantitative detection of IL-6 in each GCF sample was employed. RESULTS: No significant difference could be detected between the RA and AP groups in the mean clinical parameter data except PI. Although the mean GCF IL-6 level in the RA group was the highest, no significant difference could be found among the groups. There was only a strong negative correlation between GCF IL-6 levels and GI scores in the RA group. CONCLUSIONS: In the patients with RA, despite increased local tissue destruction potential due to autoimmunity and higher PI levels than in the AP patients, our findings suggest that medication including corticosteroid and non-steroidal anti-inflammatory drugs may decrease gingival inflammation, but the synthesis and degradation of IL-6 in gingival tissue of RA patients may be different. To our knowledge, this study is the first report determining GCF IL-6 levels in RA patients.     

袋口法重复取样测定龈沟液中酶水平

GCF量极微,如重复采取的样本能恢复至取样前水平,就可对一份样本进行多成份或一种成份的多方法检测.目的:重复取GCF样本,观察两次样本的GCF量和AST、ALP水平的变化,期望寻到能够获得三者再蓄积的较短间隔期.方法:选择未治疗的17名AP和RPP患者的100个位点,对其间隔20分钟用袋口法重复取30秒GCF样本.结果:第2次样本的AST水平显著低于第1次,GCF量和ALP水平的均值则与第1次无差别,但实际仅个别位点的GCF量和ALP能够基本或近于完全恢复,恢复的位点数分别为18.2%和24.7%.提示:重复取样的间隔期宜再延长.     

Prevalence of Bacteroides forsythus, Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in subgingival microflora of Japanese patients with adult and rapidly progressive periodontitis

BACKGROUND, AIMS: This study investigated the prevalence of Bacteroides forsythus, Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans among various periodontitis patients and healthy individuals in Japan, and correlated it with clinical parameters. METHOD: Subgingival plaque samples were collected from 21 patients with adult periodontitis (AP), 8 with rapidly progressive periodontitis (RPP) and 15 healthy individuals. RESULTS: The frequency detected in culture was as follows: B. forsythus was found in 47.6% of AP sites and in 37.5% of RPP sites. P. gingivalis was identified in 64.3% of AP and 59.4% of RPP sites. A. actinomycetemcomitans was detected in 4.8% of AP and 3.1% of RPP sites. The 3 species were detected in only 2 of the healthy individuals. The proportion of B. forsythus in the total microflora in culture was 0.07% in the healthy group, 4.1% in AP and 2.4% in RPP. The proportions of P. gingivalis were 0% in the healthy group, 18.8% in AP and 16.2% in RPP. The proportion of A. actinomycetemcomitans was very low in all 3 groups. A DNA probe detected B. forsythus in 78.6% of AP and 65.6% of RPP sites, as well as P.gingivalis in 58.3% of AP and 59.4% of RPP sites. A. actinomycetemcomitans was detected in only 1.2% of AP sites. The 3 species were undetectable in the healthy group. CONCLUSIONS: The prevalence and the proportion of B. forsythus and P. gingivalis were significantly correlated with clinical parameters, suggesting that B. forsythus and P. gingivalis are closely related to AP and RPP in the Japanese population.     

快速进展性牙周炎牙龈组织中单核/巨噬细胞浸润与Mcp-1表达

目的 研究快速进展性牙周炎牙龈组织中的单核／巨噬细胞浸润与单核细胞趋化蛋白１（ｍｏｎｏｃｙｔｅ ｃｈｅｍｏａｔｔｒａｃｔａｎｔ ｐｒｏｔｅｉｎ－１,ＭＣＰ－１）表达有无异常变化。方法 采用免疫组织化学ＳＰ染色法,取１４例快速进展性牙周炎、６例慢性牙周炎和６例健康牙龈组织,分别用抗ＣＤ１４、ＣＤ６８单克隆抗体检测牙龈组织中的单核／巨噬细胞,用ＭＣＰ－１多克隆抗测于龈组织中ＭＣＰ－１的表达。结果 快速