D2-40在乳腺癌中的表达及临床意义

目的探讨一种新的淋巴管特异标记物D2-40在乳腺癌中的作用及其临床病理意义。方法应用单克隆抗体D2-40检测15例正常乳腺组织和53例乳腺癌的淋巴管密度(LVD)的情况,并分析其和临床病理参数之间及和腋窝淋巴结转移之间的关系。结果D2-40选择性地表达在乳腺的淋巴管内皮细胞,淋巴结转移阳性组淋巴管密度明显高于淋巴结转移阴性组和正常乳腺组织,并且癌灶边缘淋巴管密度显著高于癌组织中间的密度,差异有统计学意义。结论D2-40抗体的应用更敏感和特异地检测出肿瘤的淋巴管密度,并且在预测淋巴结转移方面有帮助,因此具有良好的临床病理应用价值。     

贲门腺癌中VEGF-C、VEGF-D和D2-40的表达及意义

目的 以一种新的淋巴管特异标记物D2-40标记贲门腺癌(gastric cardiac adenocarcinoma, GCA)及癌周组织淋巴管,并进行淋巴管计数,研究其与VEGF-C 、VEGF-D表达的关系及其临床病理意义.方法应用免疫组化SP法检测贲门癌、癌旁及正常黏膜组织中VEGF-C、VEGF-D、D2-40的表达情况.结果 癌及癌旁组中淋巴管密度(lymphatic vessel density,LVD)明显高于正常组(P<0.05),而癌组与癌旁组间LVD统计学无明显差异.癌组织中LVD与淋巴结转移、TNM分期及肿瘤浸润深度呈正相关(P<0.01),而与分化程度无关;VEGF-C、VEGF-D在贲门癌中的表达明显高于癌旁组和正常组(P<0.01);癌组织中LVD在VEGF-C和VEGF-D表达阳性组中高于阴性组(P<0.05);LVD与VEGF-C、VEGF-D的表达呈正相关(P<0.05).结论 贲门癌及癌周间质中D2-40标记的LVD升高可能增加了贲门癌淋巴结转移的风险;D2-40、 VEGF-C、VEGF-D的联合检测可作为评估贲门癌淋巴结转移潜能的标志物,并且对其预后评估有一定的价值.     

血管内皮生长因子-A与血管内皮生长因子-C在非小细胞肺癌中表达及与淋巴结转移的相关性

目的 探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)-A和VEGF-C在非小细胞肺癌(non-small lung cancer,NSCLC)中的表达及与淋巴管生成、转移的关系.方法 以60例肺癌组织作为实验组,20例肺正常组织作为参考组,采用免疫组织化学方法检测其中的VEGF-A和VEGF-C两种蛋白表达,以D2-40 及CD34分别标记组织淋巴管和血管中的内皮细胞,并记录淋巴管的密度,血管作为对比,结合NSCLC临床、病理参数系统分析.结果 ①肺癌组织内VEGF-A蛋白阳性的表达率为73.33%(44/60)明显高于肺正常组织25.00%(5/20)(χ2=14.7641,P=0.0001),VEGF-C蛋白的阳性表达率为83.33%(50/60) 明显高于肺正常组织30.00%(6/20)(χ2=20.3175,P =0.0001).②肺癌组织VEGF-A蛋白阳性的表达高于癌旁周围组织(χ2=4.4815,P=0.0343),癌组织内VEGF-C蛋白阳性的表达高于癌旁周围组织(χ2=8.5333,P=0.0035).③VEGF-A与VEGF-C蛋白的表达和患者的性别、年龄大小、分化的程度、肿瘤大小、组织学无关,但淋巴结转移与PTNM分期呈显著相关(χ2=6.3736,P=0.0116)和(χ2=6.6516,P=0.0099).④VEGF-A蛋白阳性组织中微淋巴管密度(microlymphatic vessel density,MLVD)显著高于阴性组织(t=-7.2735,P<0.005),VEGF-C蛋白阳性的组织中MLVD 显著高于阴性组织(t=6.9338,P<0.005).MLVD与淋巴结转移和PTNM分期显著相关(t=-12.1146,P<0.05).结论 NSCLC组织中VEGF-A与VEGF-C二者蛋白的表达可能通过促进增加淋巴管生成从而促进淋巴结的转移.因此,在NSCLC中VEGF-A和VEGF-C蛋白可作为评估淋巴结转移的重要标记因子.     

喉鳞状细胞癌中BRAP和VEGF-C的表达及其与淋巴管生成的关系

目的探讨BRAP和VEGF-C在喉鳞状细胞癌(laryngeal squamous cell carcinoma, LSCC)组织中的表达及其与组织内淋巴管生成及淋巴结转移的关系。方法采用免疫组化PV 6000法检测84例LSCC组织及15例癌旁组织中BRAP、VEGF-C的表达,并用D2-40免疫组化染色标记检测微淋巴管生成情况。分析BRAP、VEGF-C蛋白表达与淋巴管生成及淋巴结转移的关系。结果 LSCC中BRAP、VEGF-C的高表达率分别为65.5%和53.6%,均高于癌旁组织(P0.05)。BRAP、VEGF-C在淋巴结转移组的表达均高于无淋巴结转移组(P0.05),两者的表达有相关性(P0.01)。淋巴结转移组的微淋巴管密度(micro-lymphatic vessel density, MLVD)高于无淋巴结转移组(P0.01),BRAP、VEGF-C高表达组的MLVD值比其低表达组均增高(P0.01)。结论 BRAP和VEGF-C在LSCC中呈高表达,BRAP可能通过上调VEGF-C促进肿瘤淋巴管的生成及淋巴结转移。     

VEGF-C表达与胰腺癌淋巴结转移及预后的关系

目的 观察血管内皮生长因子(VEGF)-C在胰腺癌组织内的表达情况,分析VEGF-C的表达与胰腺癌淋巴结转移和预后之间的关系。方法 取胰腺癌病例52例,其中,伴淋巴结转移组36例,无淋巴结转移组16例。应用免疫组化法和Western blot技术观察VEGF-C在胰腺癌组织内的表达。以D2-40作为淋巴管内皮特异性标记物,观察胰腺癌组织内淋巴管生成的情况。采用Kaplan-Meier法绘制生存曲线判断VEGF-C的表达对胰腺癌预后的影响。结果 Western blot和免疫组化法检测结果表明,VEGF-C主要表达于胰腺癌细胞浆内,淋巴结转移组阳性表达量明显高于无淋巴结转移组(p<0.05)。D2-40表达于胰腺癌组织内淋巴管内皮细胞,VEGF-C阳性组淋巴管数密度明显高于VEGF-C阴性组(p<0.05),表明VEGF-C的表达与胰腺癌淋巴管生成密切相关。Kaplan-Meier生存分析表明VEGF-C表达阴性患者的生存率均高于VEGF-C表达阳性患者,VEGF-C的表达影响患者的预后。结论 VEGF-C在胰腺癌的淋巴管生成和淋巴结转移过程中发挥重要作用,VEGF-C的表达是影响胰腺癌患者预后的主要因素之一。     

血管内皮生长因子D和血管内皮生长因子受体3在人结肠癌中的表达及淋巴道转移中的作用

目的 观察血管内皮生长因子D(VEGF-D)和血管内皮生长因子受体3(VEGFR-3)在人结肠癌组织中的表达,检测结肠癌组织中的微淋巴管密度(LMVD),探讨VEGF-D和VEGFR-3在淋巴管生成以及结肠癌淋巴道转移中的作用.方法 选择55例不同时期,不同分化程度的人结肠癌组织样本,应用免疫组织化学染色的方法,观察VEGF-D和VEGFR-3在人结肠癌组织中的表达,应用Podoplanin标记淋巴管,检测结肠癌组织中的淋巴管密度.结果 在55例结肠癌组织中,VEGF-D的阳性表达率为54.5%,明显高于在癌周正常组织内的表达(P＜0.05);结肠癌组织中VEGFR-3表达的阳性率为69.1%,明显高于在癌周正常组织内的表达(P＜0.01);并且VEGFR-3的表达与VEGF-D的表达具有显著相关性(P＜0.01).在结肠癌组织中,淋巴结转移阳性组,浸润深度超过肌层组,DukeC、D期的VEGF-D的表达水平和LMVD明显高于淋巴结转移阴性组,浸润深度未超过肌层组,Duke A、B期(P＜0.01),经计数淋巴管数量,癌组织中的LMVD明显高于癌周正常组织(P＜0.01),并且LMVD与VEGF-D的表达显著相关(P＜0.01).结论 结肠癌组织中VEGF-D的表达水平随着癌的浸润和转移程度的增强而增高,并且通过上调其受体VEGFR-3的表达而促进癌组织中淋巴管的生成,从而促进癌的浸润和转移.     

胰腺癌CD105和D2-40的表达及其临床病理意义

目的探讨新生血管内皮标记物CD105和淋巴管内皮标记物D2-40在胰腺癌组织中的表达及其临床病理意义。方法应用免疫组化SP法检测胰腺癌组织芯片中CD105和D2-40在88例胰腺导管腺癌和11例正常胰腺组织的表达水平,并分别计数微血管密度(MVD)和淋巴管密度(LVD),分析其与胰腺癌临床分期和有无淋巴结转移及其预后的关系。结果 88例胰腺导管腺癌组织微血管密度明显高于11例正常胰腺组织(P0.05);88例胰腺导管腺癌组织淋巴管密度明显高于11例正常胰腺组织(P0.05);胰腺癌临床分期级别越高,微血管密度越大(P0.01),有淋巴结转移的胰腺癌组织中微血管密度高于无淋巴结转移的胰腺癌组织(P0.01);胰腺癌临床分期级别越高,淋巴管密度就越高(P0.01),病理分级越高的胰腺癌组织淋巴管密度越高(P0.01),有淋巴结转移的胰腺癌组织中淋巴管密度高于无淋巴结转移的胰腺癌组织(P0.01)。结论 CD105和D2-40在胰腺导管腺癌组织中的表达显著高于正常胰腺组织,并且和胰腺癌的临床分期和淋巴结转移显著相关,二者的联合检测可能更有助于判断胰腺癌病人的预后。     

血管内皮生长因子D在卵巢上皮癌组织内的表达与淋巴结转移的关系

目的观察血管内皮生长因子D(vascular endothelial growth factor D,VEGF-D)在卵巢上皮癌组织内的表达,探讨VEGF-D在卵巢癌淋巴管生成中的作用及其与淋巴结转移的关系。方法取人卵巢上皮癌组织78例,免疫组化法观察VEGF-D在卵巢上皮癌组织内的表达情况。以淋巴管内皮特异性标记物D2-40标记淋巴管,计数癌组织内淋巴管数密度。结果VEGF-D蛋白主要表达于卵巢癌细胞胞浆内,在淋巴结转移组卵巢癌组织内的表达水平明显高于无淋巴结转移组(P<0.01)。淋巴结转移组卵巢癌组织内的淋巴管数密度明显高于无淋巴结转移组(P<0.01)。结论VEGF-D表达与卵巢癌淋巴管数密度及淋巴结转移之间具有显著的相关性。     

VEGF-C在卵巢癌局部淋巴结内淋巴管生成中的作用

目的观察血管内皮生长因子-C(VEGF-C)在卵巢癌组织内的表达,分析其与卵巢癌局部淋巴结内淋巴管生成之间的关系。方法取卵巢癌64例,其中,有淋巴结转移40例,无淋巴结转移24例。应用免疫组化法和Western blot技术观察VEGF-C在卵巢癌组织内的表达。以D2-40作为淋巴管内皮特异性标记物,检测卵巢癌局部淋巴结内淋巴管生成情况。结果 VEGF-C主要表达于卵巢癌细胞浆和胞膜以及癌组织周围浸润的炎性细胞,在有淋巴结转移组的表达率明显高于其在无淋巴结转移组的表达率。Western blot检测结果表明,VEGF-C蛋白在有淋巴结转移的卵巢癌组织中的表达量高于其在无淋巴结转移的卵巢癌组织内的表达量。D2-40表达于卵巢癌局部淋巴结内的淋巴管内皮细胞,在有转移的淋巴结内可见大量新生的淋巴管,淋巴管腔内存在入侵的肿瘤细胞,在无转移的淋巴结内观察到新生的淋巴管。在无淋巴结转移组病例中,卵巢癌组织VEGF-C阳性者局部淋巴结内淋巴管密度明显高于VEGF-C阴性者淋巴结内的淋巴管密度。结论 VEGF-C的表达与卵巢癌淋巴结转移密切相关,卵巢癌在发生局部淋巴结转移之前存在淋巴结内淋巴管生成的现象,卵巢癌组织内VEGF-C的表达在卵巢癌局部淋巴结内的淋巴管生成中可能发挥重要作用。     

Nrf2在食管鳞癌组织中的表达及意义

目的:探讨Nrf2(Nuclear factor E2 p45-related factor2)在食管鳞癌组织中的表达及其与临床病理学特征的关系。方法:采用免疫组化SP法检测Nrf2在32例食管鳞癌,30例癌旁组织,21个阳性淋巴结和24个阴性淋巴结组织中的表达。结果:Nrf2阳性表达主要定位于细胞核中,在食管鳞癌中的阳性表达率为78.13%,显著高于癌旁组织(13.33%),淋巴结癌转移阳性组织中的表达率(66.67%)也显著高于淋巴结癌转移阴性组织中的表达水平(20.83%),均具有统计学意义(P<0.05)。Nrf2的阳性表达随淋巴结的转移度的增加而表达增加(P<0.05),但在不同年龄、性别、TNM分期、肿瘤分化程度及不同部位之间差异无统计学意义。结论:Nrf2在食管鳞癌中高表达,表达的高低与淋巴结转移与否及转移度有关。     

Smad4和VEGF-C在喉癌中的表达及其与淋巴管生成之间的关系

目的观察Smad4与VEGF-C在喉癌组织内的表达情况,分析Smad4和VEGF-C的表达与喉癌组织内淋巴管生成及淋巴结转移之间的关系。方法取喉癌病例58例,其中淋巴结转移组34例,无淋巴结转移组24例。应用免疫组化法和Western blot技术观察Smad4和VEGF-C在喉癌组织内的表达。以D2-40特异性标检测喉癌组织内淋巴管生成情况。结果 Smad4在无淋巴结转移的喉癌组织内的表达率明显高于其在有淋巴结转移组的表达率。Smad4表达阳性组的淋巴管数密度(LVD)明显低于Smad4表达阴性组的LVD。VEGF-C在淋巴结转移组的表达率明显高于其在无淋巴结转移组的表达率。Smad4的表达与VEGF-C的表达呈显著的负相关性(r=-0.391)。结论 VEGF-C在喉癌淋巴管的发生及淋巴结转移中发挥重要作用。Smad4与VEGF-C的表达呈负相关,Smad4可能有抑制喉癌淋巴管生成和淋巴道转移的作用。     

趋化因子受体CCR7的表达与卵巢癌淋巴管入侵及淋巴结转移的关系

目的 观察趋化因子受体CCR7在卵巢癌组织内的表达情况,分析CCR7的表达与肿瘤淋巴管入侵和淋巴结转移之间的关系。方法 取临床资料完整的卵巢癌病例64例,其中,淋巴结转移组40例,无淋巴结转移组24例。应用免疫组化法和Western blot技术观察CCR7在卵巢癌组织内的表达。以D2-40作为淋巴管内皮特异性标记物,观察卵巢癌组织内肿瘤淋巴管入侵的情况。结果 免疫组化法和Western blot检测结果表明,CCR7表达于卵巢癌细胞浆和胞膜内,有淋巴结转移组的表达量明显高于无淋巴结转移组(p<0.05)。D2-40表达于卵巢癌组织内淋巴管内皮细胞,肿瘤淋巴管入侵在CCR7阳性组的发生率明显高于CCR7阴性组的发生率,CCR7表达与肿瘤淋巴管入侵发生率有显著相关性(p<0.05)。结论 CCR7的表达与卵巢癌发生肿瘤淋巴管入侵和淋巴结转移密切相关,提示CCR7在卵巢癌淋巴结转移中可能起重要作用。     

Smad4的表达与卵巢癌淋巴管生成及淋巴结转移之间的关系

目的观察Smad4和血管内皮生长因子(VEGF)-C在卵巢癌组织内的表达情况,分析Smad4和VEGF-C的表达与卵巢癌淋巴管生成及淋巴结转移之间的关系。方法取卵巢癌病例60例,其中,淋巴结转移组36例,无淋巴结转移组24例。应用免疫组化法和Westernblot技术观察Smad4和VEGF-C在卵巢癌组织内的表达。以D2-40作为淋巴管内皮特异性标记物,检测卵巢癌组织内淋巴管生成情况。结果 Smad4表达于卵巢癌细胞胞浆和胞核内,其在无淋巴结转移组的表达率明显高于其在有淋巴结转移组的表达率。Smad4表达阳性组的淋巴管数密度(LVD)明显低于Smad4表达阴性组的LVD。VEGF-C主要表达于卵巢癌细胞胞浆内,其在淋巴结转移组的表达率明显高于其在无淋巴结转移组的表达率。Smad4的表达与VEGF-C的表达呈显著的负相关性。Western blot检测结果表明,VEGF-C蛋白在有淋巴结转移卵巢癌组织中的表达量高于其在无淋巴结转移卵巢癌组织内的表达量,而Smad4在有淋巴结转移卵巢癌组织内的表达量明显低于其在无淋巴结转移组的表达量。结论 Smad4与VEGF-C的表达呈负相关,Smad4可能通过调节VEGF-C蛋白的表达而抑制卵巢癌淋巴管生成和淋巴道转移。     

Lateral peritumoral lymphatic vessel invasion can predict lymph node metastasis in esophageal squamous cell carcinoma.

Lymph node metastasis is an important prognostic factor in many types of cancer. We investigated the clinical significance of lymphangiogenesis and lymphatic vessel invasion in esophageal squamous cell carcinoma. We evaluated lymphatic vessel density and lymphatic vessel invasion in the intratumoral, peritumoral and normal compartments using D2-40 immunostaining. In addition, the peritumoral compartment was divided into the lateral peritumoral compartment and the non-lateral peritumoral compartment. The lymphatic vessel density was higher in the peritumoral and intratumoral compartments than in the normal compartment. However, the lymphatic vessel density did not correlate with any pathological parameters including lymph node metastasis. Intratumoral and peritumoral lymph vessels were small and collapsed while normal lymphatic vessels and lymphatic vessels with lymphatic vessel invasion were dilated and large. The presence of lymphatic vessel invasion, in the lateral peritumoral compartment but nowhere else, significantly correlated with lymph node metastasis. These results suggest that lymphangiogenesis might occur with esophageal cancer, but it does not play a direct role in lymphatic vessel invasion and lymph node metastasis. Peritumoral lymphatic vessel invasion, especially in the lateral peritumoral compartment, should imply a high probability of regional lymph node metastasis.     

D2-40 immunoreactivity in penile squamous cell carcinoma: a marker of aggressiveness

D2-40 immunohistochemical expression was investigated in tissue specimens from 39 patients with squamous cell carcinoma of the penis who underwent partial or total penectomy between 1987 and 2008. Patient age, tumor size, and grade; D2-40-positive lymphatic vessel density in intratumoral, peritumoral, and normal tissue; cell positivity for D2-40 in intratumoral and normal tissue; and D2-40 staining intensity and distribution were analyzed and correlated with disease-specific survival. Analysis of D2-40-positive lymphatics disclosed that mean lymphatic vessel density was greater in peritumoral tissue than in intratumoral and normal tissue and lower in patients with lymph node metastasis than in those without lymph node metastasis. The receiver operating characteristic curve showed that an intratumoral lymphatic vessel density greater than 2.0 had 83.3% sensitivity and 78% specificity in predicting lymph node metastasis. Analysis of cell immunoreactivity showed cytoplasmic D2-40 positivity in intratumoral and normal tissue in 89.7% and 65.5% of patients, respectively. A strong correlation emerged between grade of cell differentiation and D2-40 immunoreactivity in intratumoral tissue; in particular, 88.9% of tumors with weak podoplanin expression were G1, whereas strong cellular immunoreactivity was detected in 83.3% of G3 patients (P = .003; χ(2) test). A significant correlation was also noted between pattern of reactivity and tumor grade because the basal layer was positive in patients with undifferentiated tumors (100% of G3) and in 72.2% of G1 tumors (P = .021; χ(2) test). D2-40 seems to be a useful marker for the development of node metastasis in squamous cell carcinoma of the penis, although validation in larger series is required to confirm its predictive value.     

Lymphatic microvessel density as prognostic marker in colorectal cancer.

Lymph node metastases is an important prognostic indicator for disease progression and crucial for therapeutic strategies in the work-up of colorectal carcinoma. In this study, we investigated tumor lymphangiogenesis and vascular endothelial growth factor (VEGF) expression as predictive markers for the risk of lymph node metastasis and their relation to other prognostic parameters in colorectal carcinoma. Resected colorectal carcinomas from 90 patients were examined, including 30 patients without lymph node metastases, 30 with only lymph node metastases, and 30 with liver metastases. Cases were immunostained for CD31, D2-40, and VEGF. Positivity stained microvessels were counted in densely vascular/lymphatic foci (hot spots) at x 400 field (=0.17 mm2). Intensity of staining for VEGF was scored on a two-tiered scale. D2-40 lymphatic microvessel density demonstrated significant correlation with CD31 counts (20+/-9 vs 18+/-6/0.17 mm2 field, P<0.05) and VEGF expression (P<0.01). VEGF was expressed in 61/90 (67%) cases. D2-40 identified lymphatic tumor invasion in 48/90 patients, which was greater than CD31 (37/90) and hematoxylin and eosin (H&E) (31/90). There was a positive significant correlation of D2-40, CD31 counts, and VEGF expression with the presence of lymphovascular invasion and lymph node metastases (P<0.05). D2-40 lymphatic microvessel density correlated significantly with depth of invasion (pT), positive vascular pedicle lymph nodes and liver metastases (P<0.05). In conclusion, D2-40 lymphatic microvessel density showed prognostic significance with positive correlation with lymphovascular invasion, pT, and metastases to lymph nodes and liver. Immunostaining with D2-40 enhances the detection of lymphatic invasion relative to H&E staining and the endothelial marker, CD31.     

Intratumoral Lymphatics and Lymphatic Vessel Invasion Detected by D2‐40 Are Essential for Lymph Node Metastasis in Bladder Transitional Cell Carcinoma

Bladder cancer is frequently associated with regional lymph node metastasis at the time of diagnosis or after initial treatment, and lymph node metastasis is crucial for clinical therapeutic strategies. Lymphangiogenesis, detected by antibodies specific for lymphatic endothelial cells, is correlated with cancer spread, but the mechanisms that underlie lymphatic spread and the role of lymphangiogenesis in cancer metastasis has been less clear. The aim of this study was to investigate the association of vascular endothelial growth factor (VEGF)‐D expression, intratumoral lymphatics, and lymphatic invasion associated with lymph node metastasis as well as the prognostic analysis in patients with bladder transitional cell carcinoma (TCC). The VEGF‐D expression was evaluated by immunohistochemistry in 72 specimens, and tumoral lymphatic vessels were measured by D2‐40. Counts of lymph vessels were taken in intratumoral and peritumoral areas. Survival analyses and their independent roles were investigated using univariate and multivariate analysis models. The high expression of VEGF‐D was closely associated with the intratumoral lymphatic vessels, tumoral lymphatic invasion, and lymph node metastasis as well as a shorter overall survival. Higher lymphatic vessel density, intratumoral lymphatics, and lymphatic invasion showed a significant association with lymph node metastasis. Univariate analysis indicated that VEGF‐D, intratumoral lymphatics, and lymphatic invasion were associated with overall survival, but they were not independent prognostic factors for bladder TCC in multivariate analysis. We conclude that VEGF‐D plays an essential role in tumoral lymphangiogenesis. Intratumoral lymphatics and lymphatic invasion are important predictive factors of pelvic lymph node metastasis in patients with bladder cancer. Anat Rec, 2010. © 2010 Wiley‐Liss, Inc.     

P53和1d2蛋白在食管鳞状细胞癌的表达及其意义

目的研究P53、Id2蛋白在食管鳞状细胞癌组织的表达及其意义。方法采用免疫组织化学SP法和图像分析技术检测122例食管鳞状细胞癌及90例癌旁正常组织中P53、Id2的表达情况。结果122例食管鳞状细胞癌中P53、Id2表达水平均高于癌旁正常组织（P〈0．01）。P53表达强度与患者的性别、年龄及肿瘤的分化程度未见明显相关性（P〉0．05）,但与肿瘤的浸润深度及淋巴结转移情况相关（P〈0．05）;Id2的表达与患者的性别、年龄及淋巴结转移情况未见明显相关性（P〉0．05）,但与肿瘤的分化程度成负相关（P〈0．05）,且与肿瘤浸润深度正相关（P〈0．05）。结论P53、Id2在食管鳞状细胞癌的高表达可能作为判断食管鳞状细胞癌生物学行为的潜在指标。