白细胞介素-1B-511单核苷酸多态性与胃癌关系的流行病学研究

目的研究中国胃癌高、低发区白细胞介素（IL）-1B-511单核苷酸多态性、幽门螺杆菌（Hp）感染与胃癌的芙系。方法胃癌高发区（陕西省）胃癌患者、健康志愿者各102例，胃癌低发区（广东省）胃癌患者、健康志愿者各104例，两组人群在性别比及年龄上均匹配。采用限制性片段长度多态性（PCR—RFLP）分析IL-1B-511单核苷酸多态性，酶联免疫吸附法（ELISA）检测血清抗Hp—IgG抗体。结果在胃癌低发区，胃癌患者IL-1B-511T／T基因型频率明显高于对照人群（26．9％比13．4％，X^2=5．85，P〈0．05；OR=2．37，95％CI为1．16～4．82）。在胃癌高发区，胃癌患者IL-1B-511 T／T基因型频率与对照人群尢明显差异（27．5％比24．5％，X^2=0．41，P〉0．05）；高发区对照人群的IL-1B-511 T／T基因型频牢明显高于低发区相应人群（24．5％比13．4％，X^2=4．1，P〈0．05）。Hp感染轻度增加低发区人群发生胃癌的危险性（OR=3．03，95％CI为1．61～5．71），而IL-1B-511 T／T基因型增加Hp感染后胃癌发生的危险性（OR=8．0，95％CI为1．39～35．7）。结论IL-1B-511 T／T基因型与中国人胃癌发生有关，IL-1B-511 T／T基因型增加HP感染后胃癌发生的危险性。     

IL-1基因多态性与Hp感染后胃癌易感性的研究

目的 研究白细胞介素1B基因（IL-1B）启动子区域-31位点和-511位点及白细胞介素1受体拮抗剂基因（IL-1RN）多态性在我国北方人群胃癌患者与胃炎患者中的分布，探讨各基因型与胃癌的相关性。方法 收集126例胃癌患者与125例慢性胃炎患者（对照组）的外周血标本和流行病学资料，提取基因组DNA；IL-1RN基因采用PCR方法直接测定，IL-1B-31基因采用PCR-CTPP方法，IL-1B-511基因采用PCR-RFLP的方法进行基因分型。通过快速尿素酶、^14C呼气试验及Hp血清IgG抗体的方法检测Hp感染。结果 IL-1RN有5种基因型，分别为1／1、1／3、1／4、1／2和2／2型，其出现频率在胃癌组中分别为76．19％、4．76％、6．35％、11．90％和0．79％；在对照组分别为76．00％、4．00％、4．80％、13．60％和1．60％。各基因型在胃癌组和对照组中分布差异无统计学意义。IL-1B-31位点有3种基因型C／C、C／T和T／T型，在胃癌组中的频率分别为12．70％、47．62％和39．68％；在对照组中的频率分别为28．00％、48．80％和23．20％。与C／C型相比较，携带T／T基因型者胃癌发生的风险增加，OR=3．772（95％CI=1．786-7．966）。IL-1B-511位点有3种基因型C／C、C／T和T／T型，在胃癌组中的频率分别为19．20％、56．80％和24．00％；在对照组中的频率分别为23．38％、49．19％和27．42％。各基因型在胃癌组和对照组中分布差异无统计学意义。结论 IL-1B基因启动子区域-31位点的基因多态性可能与国人胃癌易感性相关；尚未有证据表明IL-1RN和IL-1B-511位点的基因多态性与国人胃癌易感性相关。     

白细胞介素-1基因多态性与消化性溃疡的关系

背景：大量临床流行病学证据表明消化性溃疡发病率的地域差异与宿主免疫遗传因素密切相关,目前宿主炎症因子基因多态性与消化性溃疡的关系正受到广泛关注。目的：探讨白细胞介素（IL）-1B-511、IL-1RN基因多态性与消化性溃疡的关系。方法：选取2008年9月～2009年5月昆明医学院第一附属医院确诊的57例十二指肠溃疡（DU）、38例胃溃疡（GU）以及40例非萎缩性胃炎（NAG）患者。以快速尿素酶试验和Giemsa染色检测幽门螺杆菌（H.pylori）感染,采用PCR-RFLP检测IL-1B-511、IL-1RN基因多态性。分析IL-1基因多态性、H.pylori感染、年龄与不同疾病之间的关系。结果：NAG、DU和GU组之间H.pylori感染率、IL-1B-511、IL-1RN基因型频率的差异无统计学意义。与NAG和DU相比,年龄≥60岁是GU的危险因素（OR=5.650,95%CI：1.811～17.624;OR=3.159,95%CI：1.254～7.955）。IL-1B-511、IL-1RN基因型和H.pylori感染与消化性溃疡类型无关（P〉0.05）。结论：在昆明市,年龄≥60岁是GU的危险因素,IL-1基因多态性与消化性溃疡无关。     

中国2型糖尿病人群AMPKα2基因多态性与冠心病风险的相关性研究

目的研究我国T2DM人群AMPKα2基因多态性与冠心病（CAD）风险的相关性。方法以326例T2DM患者为研究对象,其中180例伴有CAD（cAD＋组）,146例不伴CAD（CAD-组）。应用聚合酶链式反应-限制性内切酶片断长度多态性（PCR-RFLP）技术或基因测序方法,研究AMPKα2基因8个单倍型标记单核苷酸多态性（tag-SNPs）与CAD风险的关系。结果（1）SNP rs11206887 GG基因型携带者较非携带者发生CAD的风险显著增加（OR=2．507,95％CI=1．244-5．053,P=0．010）,校正年龄、性别、BMI、吸烟、糖尿病病程后仍存在统计学差异（OR′=2．469,95％CI′=1．182～5．157,P′=0．016）。（2）SNP rs2143749 GG基因型携带者较非携带者发生CAD的风险增高（OR=1．680,95％CI=1．029-2．741,P=0．038）。（3）SNP rs2746347 TT基因型携带者较非携带者发生CAD的风险有增高趋势（OR=2．875,95％CI=1．034-7．996,P=0．043,校正OR′=1．715,95％CI′=1．016-2．895,P′=0．044）。（4）SNPrs2143749和SNP rs11206887 GG／GG基因型组合携带者较非携带者发生CAD的风险增高（P=0．014）。结论我国T2DM患者AMPK α2 SNPs与CAD的发病风险可能相关。     

髓过氧化物酶基因多态性与胃癌易感性的关系

流行病学研究表明髓过氧化物酶（MPO）-463G→A多态性与多种疾病相关.目的：探讨MPO基因多态性与胃癌易感性之间的关系.方法：应用PCR-RFLP检测117例胃癌患者和105例对照者的MPO-463G→A多态性,比较两组MPO基因型频率和等位基因频率,分析MPO基因多态性与胃癌危险性的关系,并行分层分析探讨性别、年龄、吸烟状况、幽门螺杆菌（H.pylori）感染和胃癌家族史对胃癌的影响.结果：胃癌组GA和 AA基因型频率显著低于对照组（GA：25.6%对37.1%,AA：3.4%对11.4%,P〈0.05）,A等位基因频率亦明显降低（16.2%对30.0%,P〈0.05）.与GG基因型患者相比,携带MPO-463GA/AA基因型者罹患胃癌的危险性明显降低（OR=0.43,95% CI：0.25-0.75）.分层分析示男性、年龄≤60岁、不吸烟、H.pylori感染阴性和无胃癌家族史人群携带GA/AA基因型较携带GG基因型者罹患胃癌的危险性降低.结论：MPO基因多态性与胃癌易感性相关,A等位基因对胃癌易感性具有保护作用.     

白细胞介素-6基因多态性对冠心病发病易感性的影响及其机制

目的了解白细胞介素-6（IL-6）基因-597G／A及-572C／G多态性对冠心病（CHD）发病易感性的影响及其影响机制。方法 应用聚合酶链反应．限制性片段长度多态性（PCR-RFLP）分析方法,测定245例CHD患者和260例正常对照者的IL-6基因型,探讨其与CHD的相关关系;观察基因型对血清IL-6水平的影响,并采用logistic回归分析法了解基因型与CHD其他危险因素间的相互作用。结果 两组研究对象中-597位点均仅发现GG基因型。-572C／G基因型和等位基因频率在两组间存在明显统计学差异（P均〈0．01）,CHD组GG基因型和G等位基因频率均显著高于对照组（P均〈0．01）;CG、GG基因型人群患CHD的风险分别为CC基因型人群的1．46倍（95％CI：1．01～2．10,P〈0．05）和5．19倍（95％CI：1．69～15．89,P〈0．01）;不同基因型患者间血清IL-6水平无统计学差异（P〉0．05）;-572C／G基因型与总胆固醇、甘油三酯间存在一定的交互作用,OR值分别为1．76（95％CI：1．05～3．16,P〈0．05）、2．51（95％CI：1．04～6．45,P〈0．05）。结论 IL-6基因-597G／A多态性可能与中国汉族人群CHD发病易感性无关,而-572C／G多态性可能是该人群CHD发病的易感基因之一,其可能通过对组织IL-6水平的影响及与血脂的协同作用参与CHD的发生。     

胃癌易感性白细胞介素-1B基因突变检测芯片的初步临床应用

背景：白细胞介素（IL）-1B基因的单核苷酸多态性（SNP）与胃癌发生密切相关，幽门螺杆菌（H．pylori）感染增加了IL-1B基因突变型宿主胃癌发生的危险性。目的：检测IL-1B基因-31C/T和-511T／C两个位点的多态性．分析其准确性，指导胃癌易感人群的预测和H．pylori感染的个性化治疗，以防治胃癌。方法：取158例胃癌、24例十二指肠溃疡和50例慢性胃炎患者以及98名正常对照者的外周血基因组DNA为模板，聚合酶链反应（PCR）扩增样本IL-1B基因片段，应用Outdo胃癌易感性IL-1B基因突变检测芯片分析-31C／T和-511T／C的基因多态性．并通过测序验证芯片检测的正确性。结果：胃癌组IL-1B-31TT、-511CC和-31TT/511CC基因型的频率显著低于十二指肠溃疡组、慢性胃炎组和正常对照组（21．5％对58．3％、46．0％和57．1％；20．9％对58．3％、50．0％和52．0％；9．5％对45．8％、32．0％和32．7％）（P〈0．05），IL-1B-31CC、-511TT和-31CC／511TT基因型的频率则显著高于十二指肠溃疡组、慢性胃炎组和正常对照组（39．9％对16．7％、24．0％和7．1％；44.3％对12．5％、16．0％和8．2％：23.4％对4．2％、10．0％和5．1％）（P〈0．05）。与测序结果相比，20例基因芯片检测的准确率为100％。结论：IL-1B基因-31T-to-C、-511C-to-T突变与胃癌易感性增加有关。Outdo胃癌易感性IL-1B基因突变检测芯片的检测结果准确可靠．值得进一步研究以确定其临床应用价值。     

血管内皮生长因子基因启动子区多态性与血管性痴呆的关系

目的研究血管内皮生长因子（VEGF）基因启动子区多态性与血管性痴呆（VD）发病的关系。方法选择中国北方汉族139例VD患者和150名健康对照者。用直接测序的方法对VEGF基因启动子区测序；用聚合酶链反摩限制性片段长度多态（PCR—RFLP）和直接电泳PCR产物的方法对VEGF基因启动子区多态性进行分型，并对所有标本进行载脂蛋白Ee4（ApoEe4）基因分型，将两组受试者分为携带和不携带ApoEe4基因亚组[ApoEe4（＋）和ApoEe4（-）组]。结果①中国北方汉族人群中的VEGF基因启动子区存在-2578C／A、-2549I／D和-1154G／A三个多态性位点。其中-2578C／A和-2549I／D存在显著的连锁不平衡，-2549I／D位点为18个碱基的插Ⅳ缺失。②多态性位点-2578C／A、-2549I／D的基因型频率、等位基因频率分布，VD组患者和健康对照组间差异无统计学意义（P〉0．05）。经过ApoEe4分层后，VD组和对照组基因型频率、等位基因频率分布差异也无统计学意义。-1154G／A位点，VD组患者的GG基因型频率、G等位基因频率分布明显高于健康对照组（P=0．037，P=0．018）。ApoEe4（-）的亚组中，这种差异同样存在（P=0．021，P=0．010）。用Logistic回归校正年龄、性别和ApoEe4基因后，显示-1154G／A位点GG基因型患者VD的发病风险是AA基因型的1．58倍（OR=1．58，95％CI：1．073—2．338，P=0．020）。（3）ApoEe4（-）的亚组中，-2549I／-1154A单体型在VD组中少于健康对照组（OR=0．536，95％CI．O．328～0．877，P=0．012）。-2549I／-1154G单体型在VD组中明显多于健康对照组（OR=1．785，95％CI：1．018～3．131，P=0．041）。在ApoEe4（＋）的亚组中差异无统计学意义。结论VEGF基因启动子区-1154G／A位点的GG基因型是VD发病的危险因素。此危险因素是独立于ApoEe4基因而影响VD发病的。     

胃癌白细胞介素-β基因多态性与胃癌关系的荟萃分析

背景：随着胃癌病因研究的深入，目前发现许多宿主因素与胃癌的形成密切相关，白细胞介素(IL)-1B基因多态性成为重要的候选因素。目的：综合评价IL-1B-511等位基因及其基因型在胃癌发生中的作用，以期较深入地揭示IL-1B基因多态性与胃癌的关系。方法：共有7篇发表于1994年1月-2003年10月期间的有关IL-1B基因多态性与胃癌关系的国内外病例对照研究纳入荟萃分析，采用Review Manager 4．2统计软件对其结果进行定量综合分析。结果：以IL-1B-511位点的C／C基因型为对照，C／T、T／T、和*／T基因型均为胃癌的危险因素，其OR值(95％CI)分别为1．78(1．51—2．10)、1．92(1．07—3．44)和1．79(1．54～2．07)，但T／T基因型经敏感性分析后结果逆转。结论：IL-1B-511位点的C／T和*/T基因型是胃癌的危险因素,但T／T基因型与胃癌的关系尚未明确。     

白细胞介素-1β基因多态性与慢性阻塞性肺疾病易感性的系统评价

目的通过Meta分析探讨IL-1β基因多态性与慢性阻塞性肺疾病（COPD）易感性的关系。方法计算机及手工检索1980年1月至2013年1月发表的关于IL-1β基因多态性和COPD易感性关系的文献资料。根据纳入及排除标准筛选文献并提取数据。Meta分析采用RevMan5．0．25和Stata11．0软件进行。合并效应采用比值比（OR）和95％可信区间（95％CI）进行评价。发表偏倚通过漏斗图直观判断和Egger回归法、Begg秩相关法定量检测。敏感性分析为剔除不符合H—W平衡的文献后重新进行Meta分析。5篇文献（6项研究）被纳入Meta分析,共有749例COPD患者及923例对照纳入研究。结果Meta分析结果表明,IL-1β-511C／T基因多态性与COPD易感性无关联（TvsC：OR=0．97,95％CI=0．76～1．24：TTvsCC：OR：0．93,95％CI=0．55—1．59;CT＋TYvsCC：OR=1．25,95％CI=0．98～1．58;TTvsCT＋CC：OR=0．82,95％CI：0．64—1．05）,IL-1β-31T／C基因多态性与COPD易感性亦无明显联系（CUST：OR=0．99,95％CI=0．86～1．15;CCvsTF：OR=0．99,95％CI=0．72～1．35;CT＋TTvsCC：OR=1．21．95％CI=0．94—1．55;TTvsCT＋CC：OR=0．80,95％CI=0．63～1．03）。结论IL-1β-511C／T、-31T／C基因多态性与COPD易感性无关。     

Interleukin 1beta polymorphisms increase risk of hypochlorhydria and atrophic gastritis and reduce risk of duodenal ulcer recurrence in Japan

BACKGROUND & AIMS: Interleukin-1 beta (IL-1beta) polymorphisms are associated with increased risk of gastric cancer in whites. This study aimed to examine effects of these polymorphisms on gastric acid secretion, atrophic gastritis, and risk of peptic ulcer in Japan. METHODS: We determined IL-1B-511/-31 and IL-1RN genotypes and measured gastric juice pH, serum pepsinogen (PG) I and II levels, and gastritis and atrophy scores in Helicobacter pylori-positive patients with gastritis only, gastric ulcers, or duodenal ulcers (DUs), and H. pylori-negative controls. RESULTS: In the H. pylori-positive group, subjects with the proinflammatory IL-1B-511 T/T genotype had the highest atrophy and gastritis scores, the highest median gastric juice pH, and the lowest median serum PG I/PG II ratios. Although gastric juice pH significantly increased and serum PG I and PG I/PG II ratios significantly decreased in the IL-1B-511 T/T genotype group with age, no such age-dependent changes were observed in the C/C genotype group. Changes in the C/T genotype group were intermediate. In the H. pylori-negative group, the IL-1 loci had no effect on any of the physiologic or morphologic parameters. Carriage of IL-1RN allele 2 significantly protected against DU disease while the IL-1B-511 T/T genotype significantly protected against DU recurrence in patients older than 60 years. CONCLUSIONS: Proinflammatory IL-1beta polymorphisms are associated with hypochlorhydria and atrophic gastritis in Japan. The effects are dependent on H. pylori infection and become more significant with advancing age. This may explain the high incidence of gastric cancer in Japan and also the age-dependent decrease in DU recurrence in infected subjects.     

Association between interleukin-1 gene polymorphisms and Helicobacter pylori infection in gastric carcinogenesis in a Chinese population

BACKGROUND AND AIM: Helicobacter pylori is a major cause of chronic gastritis and peptic ulcer disease and a definite carcinogen for gastric adenocarcinoma. However, the underlying pathogenic mechanisms are not fully understood. Interleukin-1 (IL-1) is a key cytokine involved in H. pylori-induced gastric inflammation. The present study aimed to determine polymorphisms of IL-1B and IL-1 receptor antagonist (IL-1RN) genes and their association with H. pylori infection and gastroduodenal diseases in Chinese patients. METHODS: Three hundred and ninety-nine patients with gastroduodenal diseases (129 chronic gastritis, 127 duodenal ulcer and 143 non-cardiac gastric cancer) and 264 healthy controls were genotyped for IL-1B-511 and IL-1RN gene polymorphisms by the PCR-RFLP method. H. pylori infection status was determined by a validated serological test. RESULTS: The frequency of IL-1B-511 T allele was significantly higher in H. pylori positive patients with non-cardiac gastric cancer than in both H. pylori negative patients with non-cardiac gastric cancer (60%vs 46%, P = 0.0342, OR = 1.666, 95% confidence interval [CI]: 1.045-2.656) and in healthy controls (60%vs 48%, P = 0.0071, OR = 1.665, 95%CI: 1.149-2.412). However, the polymorphism was not associated with chronic gastritis and duodenal ulcer. Multivariate logistic regression analyses identified that IL-1B-511 T/T carrier status was an independent risk factor for non-cardiac gastric cancer in the presence of H. pylori infection (adjusted OR = 3.01, 95%CI: 1.27-7.11, P = 0.01), and the frequency of IL-1B-511 T allele was an increased risk factor for developing gastric cancer (P = 0.03, adjusted OR = 2.29, 95%CI: 1.08-4.86). There was no association between IL-1RN gene polymorphisms and H. pylori infection and other gastroduodenal diseases. CONCLUSION: IL-1B-511 T allele is associated with H. pylori infection in non-cardiac gastric cancer in a Chinese population. The IL-1B-511 gene polymorphism appears to play an important role in gastric carcinogenesis in Chinese patients with H. pylori infection.     

hTERT基因多态性与胃癌易感性的研究

人端粒酶逆转录酶(hTERT)作为端粒酶的关键酶参与了包括胃癌在内的多种肿瘤的发生和发展,有研究发现该基因的多个单核苷酸多态性(SNP)位点与多种恶性肿瘤具有不同程度的相关性。目的:探讨hTERT基因rs2853676和rs2853677位点SNP与胃癌遗传易感性的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测297例胃癌、105例萎缩性胃炎和402例对照组患者rs2853676和rs2853677位点的基因多态性,采用病理学检查和~(13)C-尿素呼气试验检测幽门螺杆菌(Hp)感染。结果:胃癌组rs2853676位点AA基因型频率显著高于对照组(15.2%对6.5%,P=0.01),AA基因型携带者患胃癌的风险增加2.47倍(95%CI:1.46~4.16)。三组rs2853677位点CC、TC、TT基因型频率差异无统计学意义。与对照组相比,萎缩性胃炎组和胃癌组Hp感染率显著升高(64.8%、56.9%对40.3%,P均0.01),OR值分别为2.73(95%CI:1.74~4.26)、1.96(95%CI:1.44~2.67)。Logistic回归分析发现,Hp感染与基因突变无明显交互作用。结论:hTERT基因rs2853676基因多态性与胃癌遗传易感性有关,其增加胃癌的风险与Hp感染可能无关。     

The role of interleukin-1beta gene polymorphism in the gastric carcinogenesis]

BACKGROUND/AIMS: This study was aimed to investigate the polymorphism of interleukin-1beta(IL-1B) and IL-1 receptor antagonist (IL-1RN) gene and the relationship between genotypes and development of gastric adenocarcinoma in Korean, and to investigate the role of Helicobacter pylori (H. pylori) infection. METHODS: The study population comprised of 258 patients with gastric adenocarcinoma. They were classified according to Lauren's classification and the status of H. pylori infection. Genomic DNA was extracted from the gastric tissue. As a control, genomic DNA from peripheral lymphocyte of 100 healthy individuals was used. The amplified products of -511 bp and -31 bp fragments in the IL-1B by PCR were digested by restriction enzyme and separated for RFLP. Variable number tandem repeats were amplified and subjected to RFLP of IL-1RN. RESULTS: There was no significant difference in the genotype of IL-1B-511T and IL-1B-31C between the adenocarcinoma group and the control group. IL-1RN allele 1 homozygote in the intestinal type showed high frequency of 91.7% (p=0.007). In the H. pylori-positive group of the adenocarcinoma, the frequency of IL-1B-31C was significantly higher than that of H. pylori-negative group (p=0.045). CONCLUSIONS: The single nucleotide polymorphism of IL-1B-31C may contribute to the development of the gastric adenocarcinoma in the H. pylori-positive population.     

Association of interleukin 1B gene polymorphism and gastric cancers in high and low prevalence regions in China

AIM: Our aim was to study the relationship between interleukin 1B (IL-1B) polymorphism, Helicobacter pylori infection, and gastric cancer in high prevalent (Shanxi) and low prevalent (Guangdong) regions in China. METHOD: Genomic DNA was extracted from peripheral blood of 192 healthy volunteers, 84 gastric cancer patients from Guangdong and 169 healthy volunteers, and 86 gastric cancer patients from Shanxi. Polymorphisms in IL-1B that encodes IL-1beta and IL-1RN that encodes IL-1 receptor antagonist were analysed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). These polymorphic sites include promoter regions of IL-1B at positions +3954, -511 (C-T transition), and -31 (T-C transition), and IL-1RN variable tandem repeats. RESULTS: In the low prevalence region, the frequencies of the IL-1B +3954 T/T and IL-1RN *2/*2 genotypes were similar. IL-1B -511T/T genotype frequency was significantly higher among patients with gastric cancer (25.0%) than control subjects (12.5%) (chi2=6.7, p=0.01). In the high prevalence region, the frequencies of the IL-1B +3954T/T and -511T/T genotypes and the IL-1RN *2/*2 genotype in the cancer and control groups were similar. IL-1B -31C/C genotype frequency was significantly higher among patients with gastric cancer (90.0%) than controls (78.0%) (chi2=5.0, p=0.025). Compared with the low prevalence region, control subjects from the high prevalence region had a higher frequency of the IL-1B -511T/T genotype (23.0% v 12.5%; chi2=7.0, p<0.008). While H pylori infection alone had only a modest effect on the risk of gastric cancer development (odds ratio (OR) 5.0 (95% confidence interval (CI) 1.5-16.3)), combined with the IL-1B -511T/T genotype the risk was markedly elevated (OR 17.1, 95% CI 3.8-76.4). CONCLUSION: IL-1B -511T/T genotypes are associated with gastric cancer in China. The effect of IL-1B polymorphism is less obvious in areas of high prevalence for gastric cancer.     

Role of environmental and genetic factor interaction in age-related disease development: the gastric cancer paradigm

The association of Helicobacter pylori (Hp) infection with gastric cancer is well known and might be considered a paradigmatic example of the role that interaction among environmental factors and individual background might play in inducing age-associated disease. To evaluate the role of interaction of Hp infection with genetic background, gastric cancer and chronic gastritis patients as well as random selected controls were typed for five inflammation-related polymorphisms of IL-1 and IL-10 cytokine genes. No association among IL-10 or IL-1 variants with an increased risk of gastric cancer was found, whereas an Hp-independent association of IL-1beta -511T positive genotypes to an increased risk of chronic gastritis was found (Hp-/511T+ OR 1.89, 95% CI: 1.01-3.54; Hp+/-511T+ OR 1.83, 95% CI: 1.05-3.19). Stratification of gastric cancer group according to Hp infection does not allow finding a statistically significant association of Hp+ to the higher histological grading (G3) of gastric cancer (OR 1.54, 95% CI: 0.46-5.11). Our findings seem to confirm that cytokine genetic variants might contribute to determining the background for inflammaging in which H. pylori infection might facilitate cancer development.     

IL-10基因单核苷酸多态性与福建地区非贲门胃癌的相关性研究

目的探讨白细胞介素10（interleukin10，IL-10）启动子区3个位点（IL-1082／-819／-592）的单核苷酸多态性、Mpylori感染与福建地区非责门胃癌之间的相关性。方法采用PCR和直接测序分析来检测IL-10基因多态性；采用胃黏膜快速尿素酶实验检测幽门螺旋杆菌。结果①IL-10．1082位点：胃癌组中A／A型（17．4％）、A／G型（26．4％）、G／G型（9．4％）与对照组相比有统计学意义（P〈0．05）。②IL-10-592位点与IL-10-819位点：胃癌组中C／C基因型（44．8％），C／A基因型（31．2％），A／A基因型（24％）与对照组相比无统计学意义（P〉0．05）。③胃癌患者IL-10-1082、IL-10-592和IL-10-819等位基因分布在H．pylori感染阳性组与阴性组之间，差异无统计学意义（P〉0．05）。结论①IL-10-1082A等位基因与福建地区胃癌发生相关。②IL—10—819／592等位基因与胃癌发生无相关。③IL-10．1082位点、IL-10-819／-592位点等位基因与执pylori感染之间无相关。