Cytidine-stabilized copper nanoclusters as a fluorescent probe for sensing of copper ions and hemin

We reported a sensitive and selective fluorescence “turn on–off” strategy for detection of Cu²⁺ and hemin, respectively. The fluorescence “turn on” sensor for Cu²⁺ detection had a wide linear range of 0.05–2.0 μM with a limit of detection (LOD) of 0.032 μM, and the fluorescence “turn off” sensor for hemin detection possessed a wide linear range of 0.05–4.0 μM with an LOD of 0.045 μM. The sensor for Cu²⁺ or hemin exhibited high selectivity over other possible substances. In addition, it was demonstrated by using various analytical characterization techniques that the fluorescence “turn on” sensor for Cu²⁺ was constructed on the basis of the formation of water-soluble fluorescent copper nanoclusters (CuNCs), and the fabrication of the fluorescence “turn off” sensor for hemin was predominately based on the inner filter effect of hemin on the fluorescence of the CuNCs. Finally, the proposed fluorescence “turn on–off” sensor system was successfully applied for detection of Cu²⁺ in lake water samples and hemin in duck blood samples.

A sensitive and selective fluorescence “turn on–off” strategy for simultaneous detection of Cu²⁺ and hemin was proposed on the basis of the formation of fluorescent CuNCs and the inner filter effect of hemin on the fluorescence of the CuNCs.     

Bis-BODIPY linked-triazole based on catechol core for selective dual detection of Ag+ and Hg2+

Herein, we introduced a new chemosensor, bis-BODIPY linked-triazole based on catechol (BODIPY-OO) prepared by bridging two units of BODIPY fluorophore/triazole binding group with a catechol unit. A solution of this compound displayed 4- and 2-fold enhancements in fluorescence intensity after adding a mole equivalent amount of Ag⁺ and Hg²⁺ ions in methanol media, respectively. ¹H NMR titrations of BODIPY-OO with Ag⁺ and Hg²⁺ suggested that the triazole was involved in the recognition process. BODIPY-OO showed high sensitivity toward Ag⁺ and Hg²⁺ over other metal ions with detection limits of 0.45 μM and 1 μM, respectively. It can also distinguish Hg²⁺ from Ag⁺ by addition of an EDTA. This compound can therefore be employed as practical fluorescent probe for monitoring the presence of Ag⁺ and Hg²⁺ ions.

BODIPY–triazole–catechol combination serves as a “turn-on” fluorescent probe for dual detection and differentiation of Hg²⁺ and Ag⁺ ions.     

An ‘‘off–on–off’’ sensor for sequential detection of Cu2+ and hydrogen sulfide based on a naphthalimide–rhodamine B derivative and its application in dual-channel cell imaging

A novel colorimetric and fluorometric sensor with unique dual-channel emission to sequentially detect Cu²⁺ and hydrogen sulfide (H₂S) was synthesized from naphthalimide–rhodamine B through the PET and FRET mechanism. The sensor showed a selective “off–on” fluorescence response with a 120-fold increase toward Cu²⁺, and its limits of detection were 0.26 μM and 0.17 μM for UV-vis and fluorescence measurements, respectively. In addition, 1–Cu²⁺ was an efficient “on–off” sensor to detect H₂S with detection limits of 0.40 μM (UV-vis measurement) and 0.23 μM (fluorescence measurement), respectively. Furthermore, the sensor can also be used for biological imaging of intracellular staining in living cells. Therefore, the sensor should be highly promising for the detection of low level Cu²⁺ and H₂S with great potential in many practical applications.

A novel colorimetric and fluorometric sensor with unique dual-channel emission to sequentially detect Cu²⁺ and hydrogen sulfide (H₂S) was synthesized from naphthalimide–rhodamine B through the PET and FRET mechanism.     

Integration detection of mercury(ii) and GSH with a fluorescent “on-off-on” switch sensor based on nitrogen,sulfur co-doped carbon dots

Using aurine and citric acid as precursors, we have synthesized stable blue-fluorescent nitrogen and sulfur co-doped carbon dots (NS-CDs), with a high quantum yield of up to 68.94% via a thermal lysis method. The fluorescent NS-CDs were employed as a sensitive sensor for the integration detection of Hg²⁺ and glutathione (GSH). This was attributed to Hg²⁺ effectively quenching the fluorescence of the NS-CDs by static quenching, and then GSH was able to recover the fluorescence owing to the stronger binding between Hg²⁺ and the sulfhydryl of GSH. Based on the “on-off-on” tactic, the detection limits of Hg²⁺ ions and GSH were 50 nM and 67 nM respectively. The fluorescence sensor was successfully applied to detect Hg²⁺ ions and GSH in actual samples (tap water and fetal bovine serum). Furthermore, we have proved that the sensor had good reversibility. Overall, our NS-CDs can serve as effective sensors for environmental and biological analysis in the future.

NS-CDs are employed as a sensitive sensor for the integration detection of Hg²⁺ and GSH. Hg²⁺ effectively quenching the fluorescence by static quenching. GSH is able to recover the fluorescence owing to the stronger binding between Hg²⁺ and GSH.     

A reasonably constructed fluorescent chemosensor based on the dicyanoisophorone skeleton for the discriminative sensing of Fe3+ and Hg2+ as well as imaging in HeLa cells and zebrafish

In this study, a new fluorescent sensor dicyanoisophorone Rhodanine-3-acetic acid (DCI-RDA) (DCI-RDA) has been developed by employing a DCI-based push–pull dye as the fluorophore and RDA as the recognition moiety for the simultaneous sensing of Fe³⁺ and Hg²⁺ with a large Stokes Shift (162 nm), high selectivity and sensitivity, and low LOD (1.468 μM for Fe³⁺ and 0.305 μM for Hg²⁺). In particular, DCI-RDA has a short response time (30 s). The Job''s plot method in combination with ¹H NMR titration and theoretical calculations was used to determine the stoichiometry of both DCI-RDA-Fe³⁺/Hg²⁺ complexes to be 1 : 1. Moreover, DCI-RDA is applied as a fluorescent probe for imaging in HeLa cells and zebrafish, indicating that it can be potentially applied for Fe³⁺/Hg²⁺ sensing in the field of biology.

A new fluorescent sensor dicyanoisophorone rhodanine-3-acetic acid has been developed by employing a DCI-based push–pull dye as the fluorophore and RDA as the recognition moiety for the simultaneous sensing of Fe³⁺ and Hg²⁺.     

Simultaneous detection of acetaminophen and 4-aminophenol with an electrochemical sensor based on silver–palladium bimetal nanoparticles and reduced graphene oxide

In this paper, Ag–Pd bimetallic nanoparticles uniformly distributed on reduced graphene oxide (rGO) were synthesized by redox reaction between Pd²⁺, Ag⁺and GO, and were characterized by X-ray diffractometry, field emission scanning electron microscopy, electrochemical impedance spectroscopy and thermal gravimetric analyses. A novel electrochemical sensor was constructed based on these nanocomposites using glassy carbon as a substrate. Under optimal conditions, the linear ranges were 0.50–300.00 μM for PA and 1.00–300.00 μM for 4-AP, with the detection limits of 0.23 μM for PA and 0.013 μM for 4-AP, respectively. This sensor was successfully applied to the determination of PA in pharmaceutical formulations and gave satisfactory results with a lower detection limit, wider linear range and good reproducibility.

Simultaneous detection of acetaminophen and 4-aminophenol with a highly sensitive electrochemical sensor based on silver–palladium bimetal nanoparticles and reduced graphene oxide.     

Sensitive electrochemical sensor based on poly(l-glutamic acid)/graphene oxide composite material for simultaneous detection of heavy metal ions

Heavy metal pollution can be toxic to humans and wildlife, thus it is of great significance to develop rapid and sensitive methods to detect heavy metal ions. Here, a novel type of electrochemical sensor for the simultaneous detection of heavy metal ions has been prepared by using poly(l-glutamic acid) (PGA) and graphene oxide (GO) composite materials to modify the glassy carbon electrode (GCE). Due to the good binding properties of poly(l-glutamic acid) (PGA) for the heavy metal ions (such as Cu²⁺, Cd²⁺, and Hg²⁺) as well as good electron conductivity of graphene oxide (GO), the heavy metal ions, Cu²⁺, Cd²⁺, and Hg²⁺ in aqueous solution can be accurately detected by using differential pulse anodic stripping voltammetry method (DPASV). Under the optimized experiment conditions, the modified GCE shows excellent electrochemical performance toward Cu²⁺, Cd²⁺, and Hg²⁺, and the linear range of PG/GCE for Cu²⁺, Cd²⁺, and Hg²⁺ is 0.25–5.5 μM, and the limits of detection (LODs, S/N ≥ 3) Cu²⁺, Cd²⁺, and Hg²⁺ are estimated to be 0.024 μM, 0.015 μM and 0.032 μM, respectively. Moreover, the modified GCE is successfully applied to the determination of Cu²⁺, Cd²⁺, and Hg²⁺ in real samples. All obtained results show that the modified electrode not only has the advantages of simple preparation, high sensitivity, and good stability, but also can be applied in the field of heavy metal ion detection.

A novel electrochemical sensor with high stability and good reproducibility for the simultaneous detection of heavy metal ions was prepared by using PGA/GO to modify the GCE, showing high sensitivity of superior to most of the reported values.     

Rapid electrochemical quantification of trace Hg2+ using a hairpin DNA probe and quantum dot modified screen-printed gold electrodes

Rapid, simple, sensitive and specific approaches for mercury(ii) (Hg²⁺) detection are essential for toxicology assessment, environmental protection, food analysis and human health. In this study, a ratiometric hairpin DNA probe based electrochemical biosensor, which relies on hairpin DNA probes conjugated with water-soluble and carboxyl functionalized quaternary Zn–Ag–In–S quantum dot (QD) on screen-printed gold electrodes (SPGE), referred to as the HP-QDs-SPGE electrochemical biosensor in this study, was developed for Hg²⁺ detection. Based on the “turn-off” reaction of a hairpin DNA probe binding with a mismatched target and Hg²⁺ through the formation of T–Hg²⁺–T coordination, the HP-QDs-SPGE electrochemical biosensor can rapidly quantify trace Hg²⁺ with high ultrasensitivity, specificity, repeatability and reproducibility. The conformational change of the hairpin DNA probe caused a significant decrease in electrochemical intensity, which could be used for the quantification of Hg²⁺. The linear dynamic range and high sensitivity of the HP-QDs-SPGE electrochemical biosensor for the detection of Hg²⁺ was studied in vitro, with a broad linear dynamic range of 10 pM to 1 μM and detection limits of 0.11 pM. In particular, this HP-QDs-SPGE electrochemical biosensor showed excellent selectivity toward Hg²⁺ ions in the presence of other metal ions. More importantly, this biosensor has been successfully used to detect Hg²⁺ in deionized water, tap water, groundwater and urine samples with good recovery rate and small relative standard deviations. In summary, the developed HP-QDs-SPGE electrochemical biosensor exhibited promising potential for further applications in on-site analysis.

A ratiometric hairpin DNA probe based electrochemical biosensor, which relies on hairpin DNA probes conjugated with water-soluble and carboxyl functionalized quantum dot on screen-printed gold electrodes, was developed for Hg²⁺ detection.     

Selective determination of Ag+ in the presence of Cd2+, Hg2+ and Cu2+ based on their different interactions with gold nanoclusters

In this work, a fluorescence method was developed for selective detection of Ag⁺ in the presence of Cd²⁺, Hg²⁺, and Cu²⁺ based on gold nanoclusters (AuNCs). That is, bovine serum albumin (BSA) templated AuNCs with double emission peaks were synthesized using BSA as a protective agent. AuNCs with uniform distribution and average size between 2.0 and 2.2 nm were synthesized using a green and simple method, and showed bright orange-red fluorescence under ultraviolet light. AuNCs have two emission peaks at 450 nm and 630 nm with an excitation wavelength of 365 nm. Under alkaline conditions, Cd²⁺ can combine with the surface sulfhydryl groups of BSA–AuNCs to form Cd–S bonds, which cause AuNCs to aggregate, resulting in an increase in fluorescence intensity at 630 nm. Conversely, due to the d¹⁰–d¹⁰ metal affinity interaction, the addition of Hg²⁺ can reduce the fluorescence peak at 630 nm. Ag⁺ was reduced to Ag⁰ by gold nuclei in AuNCs, forming a stable hybrid Au@ AgNCs species with blue-shifted and enhanced fluorescence. Finally, the paramagnetic behavior of Cu²⁺ combined with BSA causes the excited electrons of the gold cluster to lose their energy via ISC, eventually leading to simultaneous quenching of the two emission peaks. The results show that the limit of detection (LOD) of Ag⁺, Hg²⁺, Cd²⁺ and Cu²⁺ is 1.19 μM, 3.39 μM, 1.83 μM and 5.95 μM, respectively.

A fluorescence method was developed for selective detection of Ag⁺ in the presence of Cd²⁺, Hg²⁺, and Cu²⁺ based on gold nanoclusters. The limit of detection for Ag⁺, Hg²⁺, Cd²⁺ and Cu²⁺ is 1.19 μM, 3.39 μM, 1.83 μM and 5.95 μM, respectively.     

A ratiometric and colorimetric probe for detecting Hg2+ based on naphthalimide–rhodamine and its staining function in cell imaging

In this work, a rhodamine derivative was developed as a colorimetric and ratiometric fluorescent probe for Hg²⁺. It exhibited a highly sensitive fluorescence response toward Hg²⁺. Importantly, studies revealed that the probe could be used for ratiometric detection of Hg²⁺, with a low detection limit of 0.679 μM. The mechanism of Hg²⁺ detection using compound 1 was confirmed by ESI-MS, ¹H NMR, and HPLC. Upon the addition of Hg²⁺, the rhodamine receptor was induced to be in the ring-opening form via an Hg²⁺-promoted hydrolysis of rhodamine hydrazide to rhodamine acid. In addition to Hg²⁺ detection, the naphthalimide–rhodamine compound was proven to be effective in cell imaging.

A new probe based on naphthalimide–rhodamine was applied in recognition of Hg²⁺ by a FRET mechanism.     

A facile imine-linked covalent organic framework doped with a carbon dot composite for the detection and removal of Hg2+ in surface water

Hg²⁺ is one of the most toxic chemical species in the water environment, and thus developing a new fluorescent covalent organic framework for both the detection and removal of Hg²⁺ is highly desirable. Herein, a fluorescent composite, termed TpPa-1 COF@CDs, was synthesized by inverse emulsion polymerization method using an imine covalent organic framework as the supporting material and carbon dots as the fluorescent sensor element. The crystallinity, porosity, rich functional receptors (hydroxyl and amino groups), thermal stability and fluorescent properties of TpPa-1 COF@CDs were characterized. The results showed that TpPa-1 COF@CDs exhibited a good detection and removal performance for Hg²⁺, which was evidenced by its high sensitivity (LOD = 0.75 μg L⁻¹), superior selectivity, large adsorption capacity (235 mg g⁻¹), fast adsorption rate (30 min equilibrium time) and good regeneration (at least five cycles). More importantly, the simple functional monomer, short reaction time and metal-free raw material made TpPa-1 COF@CDs reliable, cost effective and eco-friendly. This research demonstrated the facile construction of a functional covalent organic framework composite for water environmental remediation technologies of metal pollution.

TpPa-1 COF@CDs as a fluorescent composite exhibited good detection and removal performance for Hg²⁺. The simple functional monomer, short reaction time and metal-free raw material made TpPa-1 COF@CDs reliable, cost effective and eco-friendly.     

A highly sensitive and selective chemosensor for Pb2+ based on quinoline–coumarin

In this study, a highly sensitive and selective fluorescent chemosensor, ethyl(E)-2-((2-((2-(7-(diethylamino)-2-oxo-2H-chromene-3-carbonyl)hydrazono)methyl)quinolin-8-yl)oxy)acetate (1), was synthesized and characterized by ¹H NMR, ¹³C NMR and ESI-MS. Sensor 1 showed an “on–off” fluorescence response to Pb²⁺ with a 1 : 1 binding stoichiometry in CH₃CN/HEPES buffer medium (9 : 1 v/v). The detection limit of sensor 1 to Pb²⁺ was determined to be 0.5 μM, and the stable pH range for Pb²⁺ detection was from 4 to 8.

A highly sensitive and selective fluorescent chemosensor 1 was synthesized and used for naked eye detection of Pb²⁺.     

Environmentally friendly synthesis of photoluminescent biochar dots from waste soy residues for rapid monitoring of potentially toxic elements

Single-step environmentally friendly synthesis of biochar dots (BCDs) was developed using hydrothermal treatment of waste biomass. Using soy residue as the carbon precursor, the resultant BCDs had strong and stable photoluminescence. Characterization by atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and infrared spectroscopy indicates that the BCDs prepared were water soluble, spherical, oxygenous and nitrogen-doped carbon nanoparticles with 10–20 nm in diameter. The fluorescence quantum yield of the BCDs was 3.7%. The use of the BCDs as a very effective fluorescent probe for label-free, rapid, and selective detection of Hg²⁺ and Fe³⁺ ions was further demonstrated with good linear relationships at 0–50 μM and 10–50 μM, respectively. The minimum detection limits of Hg²⁺ and Fe³⁺ were 100 nM and 30 nM. Furthermore, the feasibility of using the BCDs for monitoring of Hg²⁺ and Fe³⁺ in open waters was also established.

Waste biomass was used as a carbon precursor to prepare photoluminescent biochar dots for economical and eco-friendly monitoring of Hg²⁺ and Fe³⁺ ions.     

Plasmonic nanoprobes based on the shape transition of Au/Ag core–shell nanorods to dumbbells for sensitive Hg-ion detection

We report a sensitive and selective localized surface plasmon resonance (LSPR) nanoprobe for the detection of mercuric ions (Hg²⁺) using gold/silver core–shell nanorods as an optical nanosubstrate. Sulfide can quickly react with silver atoms to generate Ag₂S at room temperature in the presence of oxygen. The transformation from Ag shell to Ag₂S on the nanorod surface results in its LSPR absorption band shifting to a longer wavelength, which is attributed to their different refractive indices. Interestingly, the morphology also changed from a rod-like to dumbbell shape. However, in the presence of Hg²⁺, this morphology transformation is inhibited because the sulfide reacts with free Hg²⁺ prior to the Ag atoms. The amount of Ag₂S reduced with the increasing concentration of Hg²⁺, and the absorption band shift was also decreased. According to this “rod-like to dumbbell or not” shape change, a sensitive and selective LSPR nanoprobe was established, assisted by UV-Vis absorption spectroscopy. The detection limit of this probe for Hg²⁺ was as low as 13 nM. The efficiency of this probe in complex samples was evaluated by the detection of Hg²⁺ in spiked water samples.

Sensitive plasmonic nanoprobes for the sensitive detection of mercury ions based on a “rod-like to dumbbell or not” morphology transition of the Au/Ag core–shell hybrid nanorods.     

New portable smartphone-based PDMS microfluidic kit for the simultaneous colorimetric detection of arsenic and mercury

A smartphone-based microfluidic platform was developed for point-of-care (POC) detection using surface plasmon resonance (SPR) of gold nanoparticles (GNPs). The simultaneous colorimetric detection of trace arsenic and mercury ions (As³⁺ and Hg²⁺) was performed using a new image processing application (app). To achieve this goal, a microfluidic kit was fabricated using a polydimethylsiloxane (PDMS) substrate with the configuration of two separated sensing regions for the quantitative measurement of the color changes in GNPs to blue/gray. To fabricate the microfluidic kit, a Plexiglas mold was cut using a laser based on the model obtained from AutoCAD and Comsol outputs. The colorimetric signals originated from the formation of nanoparticle aggregates through the interaction of GNPs with dithiothreitol – 10,12-pentacosadiynoic acid (DTT-PCDA) and lysine (Lys) in the presence of As³⁺ and Hg²⁺ ions. This assembly exhibited the advantages of simplicity, low cost, and high portability along with a low volume of reagents and multiplex detection. Heavy Metals Detector (HMD), as a new app for the RGB reader, was programmed for an Android smartphone to quantify colorimetric analyses. Compared with traditional image processing, this app provided significant improvements in sensitivity, time of analysis, and simplicity because the color intensity is measured through a new normalization equation by converting RGB to an Integer system. As a simple, real-time, and portable analytical kit, the fabricated sensor could detect low concentrations of As³⁺ (710 to 1278 μg L⁻¹) and Hg²⁺ (10.77 to 53.86 μg L⁻¹) ions in water samples at ambient conditions.

A smartphone-based microfluidic platform was developed for point-of-care (POC) detection using surface plasmon resonance (SPR) of gold nanoparticles (GNPs).     

A chemosensor with a paddle structure based on a BODIPY chromophore for sequential recognition of Cu2+ and HSO3−

In this study, a highly selective chemosensor ML based on a BODIPY fluorescent chromophore was synthesized for sequential recognition of Cu²⁺ and HSO₃⁻ in a CH₃OH/H₂O (99 : 1 v/v) system, which contained three recognition sites and its structure characterized by ¹H NMR, ¹³C NMR and ESI-HR-MS. The sensor ML showed an obvious “on–off” fluorescence quenching response toward Cu²⁺ and the ML-Cu²⁺ complex showed an “off–on” fluorescence enhancement response toward HSO₃⁻. The detection limit of the sensor ML was 0.36 μM to Cu²⁺ and 1.4 μM to HSO₃⁻. In addition, the sensor ML showed a 1 : 3 binding stoichiometry to Cu²⁺ and the recovery rate of ML-Cu²⁺ complex identifying HSO₃⁻ could be over 70%. Sensor ML showed remarkable detection ability in a pH range of 4–8.

A highly selective chemosensor based on a BODIPY chromophore for sequential recognition of Cu²⁺ and HSO₃⁻.     

Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione

A convenient, fast, sensitive and highly selective fluorescence sensor for the detection of glutathione (GSH) based on DNA derived bio-dots (DNA bio-dots)/polydopamine (PDA) nanoparticles was constructed. The fluorescent switch of DNA bio-dots was induced to turn off because of fluorescence resonance energy transfer (FRET) reactions between DNA bio-dots and PDA. The presence of GSH blocked the spontaneous oxidative polymerization of dopamine (DA) to PDA, leading the fluorescent switch of DNA bio-dots to be “turned on”. The degree of fluorescence recovery of DNA bio-dots is linearly correlated with the concentration of GSH within the range of 1.00–100 μmol L⁻¹, and the limit of detection (LOD) is 0.31 μmol L⁻¹ (S/N = 3, n = 9). Furthermore, the fluorescence sensor was successfully used to quantify GSH in human urine and glutathione whitening power, indicating the fluorescence sensor has potential in the detection of human body fluids and pharmaceutical preparations.

The turn-on fluorescence signal mechanism for detection of GSH.     

Sensitive distance-based paper-based quantification of mercury ions using carbon nanodots and heating-based preconcentration

This article reports the first fluorescent distance-based paper device coupled with an evaporating preconcentration system for determining trace mercury ions (Hg²⁺) in water. The fluorescent nitrogen-doped carbon dots (NCDs) were synthesized by a one-step microwave method using citric acid and ethylenediamine. The fluorescence turn-off of the NCDs in the presence of Hg²⁺ was visualized with a common black light, and the distance of the quenched fluorescence correlated to Hg²⁺ concentration. The optimal conditions for pH, NCD concentration, sample volume, and reaction time were investigated. Heating preconcentration was used to improve the detection limits of the fluorescent distance-based paper device by a factor of 100. Under the optimal conditions, the naked eye limit of detection (LOD) was 5 μg L⁻¹ Hg²⁺. This LOD is sufficient for monitoring drinking water where the maximum allowable mercury level is 6 μg L⁻¹ as established by the World Health Organization (WHO). The fluorescent distance-based paper device was successfully applied for Hg²⁺ quantification in water samples without interference from other cations. The proposed method provides several advantages over atomic absorption spectroscopy including ease of use, inexpensive material and fabrication, and portability. In addition, the devices are simple to fabricate and have a long shelf-life (>5 months).

This article reports the first fluorescent distance-based paper device coupled with an evaporating preconcentration system for determining trace mercury ions (Hg²⁺) in water.     

A colorimetric and ratiometric fluorescent sensor for sequentially detecting Cu2+ and arginine based on a coumarin–rhodamine B derivative and its application for bioimaging

In this work, a colorimetric and ratiometric fluorescent sensor based on a coumarin–rhodamine B hybrid for the sequential recognition of Cu²⁺ and arginine (Arg) via the FRET mechanism was designed and synthesized. With the addition of Cu²⁺, the solution displayed a colorimetric change from pale yellow to pink which is discernible by the naked eye. Additionally, the fluorescence intensities of the sensor exhibited ratiometric changes for the detection of Cu²⁺ at 490 and 615 nm under a single excitation wavelength of 350 nm, which corresponded to the emissions of coumarin and rhodamine B moieties, respectively. The fluorescence color change could be visualized from blue to pink. The limits of detection were determined to be as low as 0.50 and 0.47 μM for UV-vis and fluorescence measurements, respectively. More importantly, the sensor not only can recognize Cu²⁺ and form a sensor-Cu²⁺ complex but can also sequentially detect Arg with the resulting complex. The detection limits for Arg were as low as 0.60 μM (UV-vis measurement) and 0.33 μM (fluorescence measurement), respectively. A fluorescence imaging experiment in living cells demonstrated that the fabricated sensor could be utilized in ratiometric fluorescence imaging towards intracellular Cu²⁺, which is promising for the detection of low-level Cu²⁺ and Arg with potentially practical significance.

A FRET-based colorimetric and ratiometric coumarin–rhodamine B fluorescent sensor was designed, and its sensing behaviors for sequentially detecting Cu²⁺ and arginine were studied systematically.     

On-site,rapid and visual method for nanomolar Hg2+ detection based on the thymine–Hg2+–thymine triggered “double” aggregation of Au nanoparticles enhancing the Tyndall effect

This work describes a new nanosensor for the simple, rapid, portable, colorimetric analysis of mercury(ii) (Hg²⁺) ions by combining the sensitive Tyndall effect (TE) of colloidal Au nanoparticles (AuNPs) with specific thymine–Hg²⁺–thymine (T–Hg²⁺–T) coordination chemistry for the first time. For the TE-inspired assay (TEA), in the presence of Hg²⁺ in a sample, the analyte can selectively mediate the hybridization of three types of flexible single-stranded DNAs (ssDNAs) to form stable rigid double-stranded DNAs (dsDNAs) via the T–Hg²⁺–T ligand interaction. Subsequent self-assembly of the dsDNAs with terminal thiol groups on the AuNPs'' surfaces led to their “double” aggregation in addition to the lack of sufficient ssDNAs as the stabilizing molecules in a high-salt solution, resulting in a remarkably enhanced TE signal that positively relied on the Hg²⁺ level. The results demonstrated that such a TEA method enabled rapid naked-eye qualitative analysis of 625 nM Hg²⁺ within 10 min with an inexpensive laser pointer pen as an inexpensive handheld light source to generate the TE response. Making use of a smartphone for portable TE readout could further quantitatively detect the Hg²⁺ ions in a linear concentration range from 156 to 2500 nM with a limit of detection as low as 25 nM. Moreover, the developed equipment-free nanosensor was also used to analyze the Hg²⁺ ions in real samples including tap water, drinking water, and pond water, the obtained recoveries were within the range of 93.68 to 108.71%. To the best of our knowledge, this is the first report of using the AuNPs and functional nucleic acids to design a TE-based biosensor for the analysis of highly toxic heavy metal ions.

A new equipment-free colorimetric nanosensor was initially developed for quantitative point-of-need detection of nanomolar Hg²⁺ ions based on the enhancement in Tyndall effect of Au nanoparticles via their target-triggered “double” aggregation.