共查询到20条相似文献,搜索用时 15 毫秒
1.
Thanh Chung Pham Yeonghwan Choi Chaeeon Bae Cong So Tran Dongwon Kim Ok-Sang Jung Yong-Cheol Kang SungYong Seo Hyun Sung Kim Hwayoung Yun Xin Zhou Songyi Lee 《RSC advances》2021,11(17):10154
Cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) are essential biothiols for cellular growth, metabolism, and maintenance of a biological system. Thus, the detection of biothiols is highly important for early diagnosis and evaluation of disease progression. In this article, a series of sulfonyl aza-BODIPYs was synthesized, characterized, and examined by 1H-NMR, 13C-NMR, crystallization, photophysical properties and DFT calculation. Among these structures, a fluorescent probe, BDP-1, exhibited selective detection of Cys among various biothiols via nucleophilic aromatic substitution and typical size of Cys molecules. BDP-1 showed color change and near-infrared (NIR) fluorescence enhancement after reaction with Cys to generate BDP-OH, confirmed by HRMS. The red shift of absorption wavelength showed a similar tendency resulting in time-dependent density functional theory (TD-DFT). Furthermore, the calculated detection limit of BDP-1 toward Cys was 5.23 μM. This probe facilitates the colorimetric and fluorescent detection of Cys over other biothiols.A new fluorescent and colorimetric probe based-on sulfonyl aza-BODIPY (BDP-1–3) are designed and synthesized for selective cysteine detection. 相似文献
2.
Zhixiang Lu Yunming Liu Shuhan Lu Yuan Li Xiaolan Liu Yu Qin Liyan Zheng 《RSC advances》2018,8(35):19701
The detection of Ag+ in the environment is very important to determine the level of pollution from silver complexes, which have caused various human health problems. Herein, an aggregation-induced emission (AIE) chromophore (tetraphenylethane, TPE) attached to a benzimidazole group (tetra-benzimidazole, TBI–TPE) is synthesized and utilized to detect Ag+ in the environment. The strong chelating effect between the benzimidazole group and Ag+ leads to the formation of aggregates, and strong yellow fluorescence signals were observed after adding Ag+ into a TBI–TPE solution. The stoichiometry of the complex of TBI–TPE and Ag+ was established to be 1 : 2 using photochemical and mass spectra measurements. The detection limit of the Ag+ assay is 90 nM with a linear range from 100 nM to 6 μM. This study provides a facile method to determine Ag+ in real environmental samples with satisfactory results.We develop a highly selective TPE-based AIE fluorescent probe containing a benzimidazole group for the detection of Ag+. 相似文献
3.
A coumarin-based dual responsive fluorescent probe with a simple structure was developed for the detection of Cys and HSO3−. Under simulated physiological conditions, Cou-F displayed an on–off fluorescence response to Cys at 521 nm and an off–on fluorescence response to HSO3− at 500 nm. Furthermore, Cou-F had the advantages of high sensitivity, strong specificity and rapid response. The detection limits of Cou-F toward Cys and HSO3− were 0.54 μM and 0.65 μM, respectively. Cou-F enabled high selective responses to Cys and HSO3− over other biologically related species. The response times of Cou-F toward Cys and HSO3− were 80 s and 100 s. The fluorescence imaging of Cys and HSO3− was achieved in living RAW246.7 cells.A coumarin-based dual responsive fluorescent probe with a simple structure was developed for the detection of Cys and HSO3−. 相似文献
4.
Geng Yang Xia Meng Shimin Fang Hongdong Duan Lizhen Wang Zhenzheng Wang 《RSC advances》2019,9(15):8529
An efficient fluorescent probe Pyr-Rhy based on pyrazole was developed, which can detect Hg2+ in water. Its fluorescence properties were studied by UV-vis and fluorescence spectroscopy, and the study results indicated that this probe can selectively detect Hg2+via complexation reaction, and then cause a remarkable color change from colorless to pink and a strong fluorescence enhancement can be observed. Furthermore, this probe showed high sensitivity with the detection limit down to 2.07 × 10−8 M, and its stoichiometric ratio toward Hg2+ ions was 1 : 1. The sensing mechanism was investigated by Job''s plot 1H NMR titrations, and FT-IR spectra analysis, which demonstrated a chelation-enhanced fluorescence (CHEF) mechanism. More importantly, obvious color changes of sensor Pyr-Rhy can be observed when it was impregnated on filter paper testing strips and immersed in Hg2+ solution (water as solution), indicating its potential application for trace Hg2+ detection in environmental samples.An efficient fluorescent probe Pyr-Rhy based on pyrazole was developed, which can detect Hg2+ in water. 相似文献
5.
Herein, a simple two-photon turn-on fluorescent probe, N-(6-acyl-2-naphthayl)-maleimide (1), based on a dual PeT/ICT quenching mechanism is reported for the highly sensitive and selective detection of cysteine (Cys) over other biothiols. The probe was applied in the two-photon imaging of Cys in cultured HeLa cells, excited by a near-infrared laser at 690 nm. N-(6-acyl-2-naphthayl)-maleimide (1) is a simple two-photon fluorescent probe with selectivity for cysteine, based on a thiol-Michael-addition-transcyclization cascade and dual PeT/ICT quenching mechanism.Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are structurally similar biothiols, but their biological functions are quite different from one another.1–6 Among these biothiols, Cys functions as one of the twenty-one amino acids for peptide and protein synthesis, and Cys deficiency is also associated with certain disease symptoms.7–10 Methods for the selective detection and differentiation of Cys among different biothiols, including high performance liquid chromatography (HPLC),11 capillary electrophoresis,12 electrochemical assay,13 UV-vis spectroscopy,14 and fluorescence-based methods,15–17 are important for its biological studies. Recently, fluorescent probes have attracted much attention as vital chemical biology tools due to their high sensitivity, convenient operation, and real-time imaging capabilities.18–20 A number of Cys-selective fluorescent probes have been reported,21 which utilize Cys-selective recognition groups such as aldehydes,11,22 acrylates,23 thioesters,24 and electron-deficient aromatic halides25–27 in their structures. However, many of them have relatively long response times and low sensitivity due to a slow cyclization process. In addition, fluorescent probes with high selectivity for Cys over Hcy are difficult to achieve because they differ by only one methylene group.28 Recently, we reported that N-(N′-butyl-1,8-naphthalimide-4-yl)-maleimide, containing a single maleimide group as the recognition group, is a fast, sensitive, and selective fluorescent probe for Cys based on a dual photo-induced electron transfer (PeT) and photo-induced intramolecular charge transfer (ICT) quenching mechanism.28 Different from many other maleimide-based fluorescent probes that only undergo a PeT mechanism,15 the additional ICT quenching mechanism keeps the 1,8-naphthalimide (NAP) fluorophore in the thiol-Michael adduct in a low fluorescence emission state due to the strong electron-withdrawing effects of the succinimide group at its 4-position. Then, a subsequent transcyclization step, involving the formation of a six-membered thiomorpholinone ring and cleavage of a five-membered succinimide ring, converts the non-fluorescent thiol-Michael adduct into the major fluorescent product, in which the ICT quenching is removed, resulting in a drastic fluorescence turn-on response.28 A similar transcyclization process and the simultaneous removal of ICT quenching allowed us to design a NAP-based turn-on fluorescent probe for γ-glutamyltranspeptidase29 and a coumarin-based turn-on fluorescent probe with dual recognition groups and dual cyclization for the selective detection of Cys.30 In addition, another NAP-based dual PeT/ICT probe was recently reported by Meka and Heagy for the detection of hydrogen sulfide, although two recognition groups instead of one were adopted in their probe to achieve the dual quenching mechanism.31Our previous work and that of other groups has demonstrated that the combination of PeT and ICT mechanisms is particularly suitable for the design of fluorescent probes with a significant fluorescence turn-on response.30–33 However, many of these probes have a short excitation wavelength in the UV or visible range, which is not optimal for biological applications due to enhanced phototoxicity and/or autofluorescence.34,35 Considering that two-photon fluorescence imaging has advantages such as the excitation process being carried out by a near-infrared (NIR) laser that has a reduced cell toxicity and low fluorescence background,36 in this work, we aimed to introduce a similar dual PeT/ICT quenching mechanism to the known two-photon fluorophore 6-acyl-2-naphthylamine37–39 in order to design a simple maleimide-based two-photon fluorescent probe, 1, for the selective detection of Cys over Hcy and GSH. It was also tested to determine whether it is a turn-on fluorescent probe with high sensitivity and selectivity, which reacts with Cys via a fast two-step thiol-Michael addition and transcyclization cascade reaction.28 The structure of probe 1 is shown in Fig. 1. It has a maleimide group at its 2-position, which promotes the PeT quenching effect. It also has an additional electron-withdrawing methylcarbonyl group at its 6-position to ensure a pull–pull ICT quenching effect.Open in a separate windowFig. 1Design rationale of the fluorescent probe 1 for the selective turn-on detection of Cys over Hcy and GSH.Probe 1 was conveniently synthesized from 6-acyl-2-naphthylamine (3)39 in a two-step process with a total yield of 38% (see Scheme S1 in the ESI†). First, the amine 3 was reacted with maleic anhydride to form the maleic amide acid 4. Then, the amide acid 4 was cyclized to afford the maleimide 1 in the presence of acetic anhydride (see the ESI† for more details).We then investigated the absorption and fluorescence emission response of the probe towards just 1 equiv. of Cys. The time-dependent absorption spectra upon the addition of 1 equiv. of Cys are shown in Fig. 2a. Probe 1 has a maximum absorption peak at 292 nm. Upon addition of Cys, the maximum absorption peak shifts to 314 nm, a red-shift of 22 nm. Notably, an isosbestic point can be seen at 295 nm after 2 min, indicating the formation of an intermediate within 2 min, which is then converted into the final product. The UV spectral changes supported the presence of a proposed cascade reaction sequence for the fast formation of a thiol-Michael adduct intermediate, which then underwent a relatively slow intramolecular transcyclization process to give the final product. From time-dependent fluorescence emission studies (Fig. 2b), probe 1 was found to have almost no fluorescence emission due to dual PeT and ICT quenching effects. Upon the addition of 1 equiv. of Cys, a drastic turn-on fluorescence response (a >3000 fold increase) was observed at 446 nm (see Fig. S1b in the ESI†). The fluorescence intensity at 446 nm reached its maximum value after around 30 min indicating that the cascade reaction finished in about 30 min (Fig. 2b, and S2a in the ESI†). The pseudo-first-order reaction kinetic constant based on the fluorescence enhancement was calculated as 0.123 min−1 (half-time = 5.64 min, Fig. S2b in the ESI†), indicating an overall fast cascade reaction. Fluorescence titration experiments using an increasing amount of Cys from 0 to 4.0 equiv. over 30 min showed a steady increase in the fluorescence intensity and the maximum intensity was reached at exactly 1.0 equiv. of Cys. Further Cys addition did not increase the fluorescence intensity, indicating that probe 1 reacts with Cys in a 1 : 1 molar ratio (Fig. 2c and S3 in the ESI†), which was also supported by the Job plot (see Fig. S4 in the ESI†). From the linear relationship of the fluorescence intensity at 446 nm versus the Cys concentrations, the detection limit of probe 1 (2 μM) for Cys was calculated as 1.4 nM (S/N = 3, Fig. 3d), indicating that 1 is a highly sensitive probe for Cys. Moreover, the probe showed excellent selectivity for the detection of Cys over many other species (Fig. 2e, and S5 in the ESI†), including the structurally similar thiols Hcy, GSH, and N-acetylcysteine (NAC). The fluorescence intensity at 446 nm for 1 equiv. of Cys was significantly higher (12.2-fold, 9.1-fold, and 17.7-fold, respectively) than that of 10 equiv. of Hcy, GSH, or NAC. To further confirm the reaction mechanism, the reaction product, 2, from the reaction of probe 1 with Cys, was isolated and its structure was confirmed using 1H nuclear magnetic resonance (NMR) spectroscopy, 13C NMR spectroscopy, 2D-rotating-frame nuclear Overhauser effect spectroscopy (ROESY), and high-resolution mass spectrometry (HRMS) (see the ESI for more details†). The fluorescence quantum yields of probe 1 and product 2 were measured as 0.002 and 0.782, respectively (see the ESI for more details†). Therefore, the formation of the transcyclization product 2 was determined to be responsible for the observed fluorescence turn-on response. For the other thiols, the transcyclization steps of the thiol-Michael adducts were much slower, resulting in the observed high selectivity. Overall, we have shown here that probe 1 is a highly sensitive and selective turn-on fluorescent probe for Cys.Open in a separate windowFig. 2(a) Time-dependent UV-vis spectra of probe 1 (10 μM) upon the addition of 1 equiv. of Cys (a spectrum was recorded every 2 minutes); (b) time-dependent fluorescence emission spectra of probe 1 (2 μM) upon the addition of 1 equiv. of Cys (a spectrum was recorded every 3 minutes); (c) time-dependent fluorescence emission intensity at 446 nm of probe 1 (2 μM) upon addition of Cys (0 to 4 equiv.); (d) a linear relationship of the fluorescence intensity at 446 nm versus the Cys concentration (0.2–2.0 μM); (e) fluorescence response of probe 1 (2 μM) at 446 nm toward various species in PBS buffer (10 mM, pH 7.4): (1) blank; (2) Cys; (3) Hcy; (4) GSH; (5) NAC; (6) valine; (7) glycine; (8) isoleucine; (9) lysine; (10) leucine; (11) histidine; (12) asparagine; (13) methionine; (14) proline; (15) serine; (16) alanine; (17) threonine; (18) arginine; (19) glutamine; (20) aspartic acid; (21) glutamic acid; (22) tyrosine; (23) tryptophan; (24) phenylalanine; (25) glucose; (26) H2O2; (27) Na+; (28) K+; (29) Ca2+; (30) Mg2+; (31) Fe3+; (32) Fe2+; (33); Cu2+; (34) Zn2+ (All measurements were made in 10 mM PBS buffer, pH 7.4, 25 °C, and λex = 314 nm).Open in a separate windowFig. 3Two-photon fluorescence images (b, e, h, k) of HeLa cells collected at 410–510 nm (blue to cyan-blue, λex = 690 nm), the corresponding bright field view (a, d, g, j), and overlap of the fluorescence channel and the bright field view (c, f, i, l) after different treatments: (a–c) the cells were pretreated with 0.5 mM of N-ethylmaleimide (NEM) for 30 min and then incubated with 10 μM of probe 1 for 30 min; (d–f) cells were first pretreated with 0.5 mM of NEM for 30 min, then after addition of 1 mM of Cys were incubated for 30 min, and finally, incubated with 10 μM of probe 1 for 30 min (scale bar = 10 μm); the conditions for (g–i) and (j–l) were similar to those of (d–f), except that 10 μM of Hcy and 10 μM of GSH were used instead of 10 μM of Cys.Encouraged by the fast, selective, and sensitive in vitro fluorescence response of probe 1 for the detection of Cys, we further evaluated its potential use as a two-photon imaging agent for Cys in biological systems, such as in living cells. The fluorescence response of probe 1 towards Cys at different pH values was evaluated and a suitable pH range for Cys detection was determined to be 7.0 to 10.0, which is a good range for cell imaging applications because physiological conditions have a pH of around 7.4 (see Fig. S7 in the ESI†). HeLa cells were then pretreated with N-ethylmaleimide (NEM, 0.5 mM) for 30 min to remove the endogenous cellular thiols, and incubated with Cys (1 mM), Hcy (1 mM), or GSH (1 mM), respectively for 30 min to increase the specific thiol levels. The samples were then further incubated with probe 1 (10 μM) for 30 min and were then washed with PBS buffer before two-photon fluorescence cell images and the corresponding bright-field view images were taken (Fig. 3(d–l)). Control images were also taken for samples pretreated with NEM (0.5 mM) and then incubated with probe 1 (10 μM) (Fig. 3a–c). Only cells pretreated with NEM and then Cys showed a distinctive blue fluorescence (Fig. 3e). The above cell imaging studies clearly demonstrated that probe 1 is capable of the selective detection and imaging of intracellular Cys over Hcy and GSH in living cells by two-photon fluorescence imaging with low background fluorescence interference. 相似文献
6.
Lead is a highly toxic heavy metal, and various functional nucleic acid (FNA)-based biosensors have been developed for the detection of Pb2+ in environmental monitoring. However, most fluorescence biosensors that have been reported were designed on the basis of a double-labeled (fluorophore and quencher group) DNA sequence, which not only involved an inconvenient organic synthesis but also restricted their wider use in practical applications. Here, we utilized a G-rich DNA sequence as a recognition probe and conjugated fluorene (CF) to develop a fluorescence sensor without a quencher based on the aggregation-caused quenching (ACQ) effect. In the presence of Pb2+, the degree of aggregation of CF was reduced because Pb2+ induced the formation of a G-quadruplex structure of the CF-DNA probe, and the fluorescence signal increased with the concentration of Pb2+ (0–1 μM), with a limit of detection of 0.36 nM. This fluorescent probe without a quencher enables the sensitive and selective detection of Pb2+. On the basis of these advantages, the CF-DNA probe represents a promising analytical method for detecting Pb2+.Fluorescent probe with only a fluorophore but no quencher for detecting Pb2+ on the basis of the aggregation-caused quenching (ACQ) phenomenon. 相似文献
7.
A novel, highly sensitive and fast responsive turn-on fluorescence probe, 2,2′-((1E,1′E)-((1,10-phenanthroline-2,9-diyl)bis(methanylylidene)) bis(azanylylidene)) diphenol (ADMPA), for Cd2+ was successfully developed based on 2,9-dimethyl-1,10-phenanthroline and o-aminophenol. ADMPA showed a remarkable fluorescence enhancement toward Cd2+ against other competing cations, owing to the suppression of the photo-induced electron transfer (PET) and CH N isomerization. A good linear relationship (R2 = 0.9960) was obtained between the emission intensity of ADMPA and the concentration of Cd2+ (0.25–2.5 μM) with a detection limit of 29.3 nM, which was much lower than that reported in literature. The binding stoichiometry between ADMPA and Cd2+ was 2 : 1 as confirmed by the Job''s Plot method, which was further confirmed by a 1H NMR titration experiment. Moreover, the ADMPA probe was successfully applied to detect Cd2+ in real water samples with a quick response time of only 6.6 s, which was about 3–40 times faster than the reported cadmium ion probe.A novel, highly sensitive and fast responsive turn-on fluorescence probe ADMPA for Cd2+ was successfully developed based on 2,9-dimethyl-1,10-phenanthroline and o-aminophenol. 相似文献
8.
Three novel β-pinene-based fluorescent probes 2a–2c were designed and synthesized for the selective detection of Al3+. Probe 2a showed higher fluorescence intensity toward Al3+ than the other two compounds. Probe 2a determined the concentration of Al3+ with a rapid response time (45 s), wide pH range (pH = 1–9), excellent sensitivity (LOD = 8.1 × 10−8 M) and good selectivity. The recognition mechanism of probe 2a toward Al3+ was confirmed by 1H NMR, HRMS and DFT analysis. Probe 2a was successfully used as a signal tool to quantitatively detect Al3+ in food samples and environmental water samples. Furthermore, probe 2a was successfully utilized to label intracellular Al3+, indicating its promising applications in living cells.Probe 2a exhibiting high sensitivity, good selectivity, wide pH range, lower detection limit, and rapid detection for Al3+, probe 2a was applied for the successful detection of Al3+ in water samples, food samples and HeLa cells. 相似文献
9.
Yiqing Liu Ting Kang Qian He Yuefu Hu Zeping Zuo Zhihua Cao Bowen Ke Weiyi Zhang Qingrong Qi 《RSC advances》2021,11(24):14824
The disruption of copper homeostasis (Cu+/Cu2+) may cause neurodegenerative disorders. Thus, the need for understanding the role of Cu+ in physiological and pathological processes prompted the development of improved methods of Cu+ analysis. Herein, a new near-infrared (NIR) fluorescent turn-on probe (NPCu) for the detection of Cu+ was developed based on a Cu+-mediated benzylic ether bond cleavage mechanism. The probe showed high selectivity and sensitivity toward Cu+, and was successfully applied for bioimaging of Cu+ in living cells.The disruption of copper homeostasis (Cu+/Cu2+) may cause neurodegenerative disorders. 相似文献
10.
Keli Zhong Longlong Deng Jie Zhao Xiaomei Yan Tong Sun Jianrong Li Lijun Tang 《RSC advances》2018,8(42):23924
A novel near-infrared fluorescent probe (L) based on a 1,4-diethyl-1,2,3,4-tetrahydro-7H-pyrano[2,3-g]quinoxalin-7-one scaffold has been synthesized and characterized. Probe L displays highly selective and sensitive recognition to H2S over various anions and biological thiols with a large Stokes shift (125 nm) in THF/H2O (6/4, v/v, Tris–HCl 10 mM, pH = 7.4). This probe exhibits turn-on fluorescence for H2S through HS− induced thiolysis of dinitrophenyl ether. Confocal laser scanning micrographs of MCF-7 cells incubated with L confirm that L is cell-permeable and can successfully detect H2S in living cells.A novel “off–on” fluorescent probe (L) for H2S detection with NIR emission and imaging H2S in living cells. 相似文献
11.
Tetracycline (TC) is a broad-spectrum antibiotic. When humans consume too much food containing tetracycline residues, it can be a serious health hazard. Therefore, it is essential to develop a strategy to detect TC. In this study, we prepared light blue-green luminescent nitrogen-doped graphene quantum dots (N-GQDs) by a hydrothermal method using the natural products potato straight-chain starch and urea as precursors; the fluorescence quantum yield of the prepared N-GQDs was 5.2%. We investigated the detection of tetracycline (TC) by this N-GQD fluorescent sensor based on the internal filtration effect (IFE) of TC on N-GQDs. The reaction is green, simple and no other contaminating products are present. A good linear relationship was established between the relative fluorescence intensity ratio of the system and the logarithm of the TC concentration of 2.5 × 10−10 to 5 × 10−6 M (R2 = 0.9930), with a detection limit of 9.735 × 10−13 M. The method has been used to analyze TC in three real food samples (whole milk, skim milk, honey) with low detection limits (3.750 × 10−11 to 2.075 × 10−9 M), wide linear range, and satisfactory recoveries of 93.80–109.20% were obtained. In conclusion, the proposed method is a green, rapid, highly sensitive and selective method for the detection of tetracycline in real food samples, demonstrating the potential application of N-GQDs in food detection.Tetracycline (TC) is a broad-spectrum antibiotic. 相似文献
12.
Ping Ju Ensheng Zhang Long Jiang Ze Zhang Xiangyang Hou Yuqi Zhang Hua Yang Jijiang Wang 《RSC advances》2018,8(39):21671
A new three-dimensional metal–organic framework (MOF) sensor with molecular formula (C2H6NH2)2[Tb2(ptptc)2(DMF)(H2O)]·DMF·6H2O (complex 1) has been constructed from terphenyl-3,3′,5,5′-tetracarboxylic acid (H4ptptc) and terbium nitrate under solvothermal conditions. The structure of complex 1 was characterized by single-crystal X-ray diffraction analysis (XRD), elemental analysis, IR spectroscopy and thermogravimetric (TG) analysis, and the purity was further confirmed by powder X-ray diffraction (PXRD) analysis. XRD analysis reveals that complex 1 crystallizes in a triclinic system P space group and consists of a three-dimensional anionic network which has one-dimensional channels. Fluorescence titration experiments showed that complex 1 displayed real-time, highly selective and sensitive fluorescence quenching behavior towards picric acid with a nanomolar scale experimental detection limit (100 nM). Recycling titration experiments suggested that the as-synthesized probe has good reversibility and can be used for at least five cycles in fluorescence titration experiments without obvious fluorescence intensity reduction or framework structure destruction. Furthermore, the high selectivity and sensitivity as well as good recyclability of complex 1 make it a potential fluorescent sensor for picric acid.A new three-dimensional reusable Tb-MOF fluorescent sensor for the highly selective and sensitive detection of picric acid was reported. 相似文献
13.
Zujun Xu Wenxiu Shi Chengjun Yang Jing Xu Huapeng Liu Jing Xu Baocun Zhu 《RSC advances》2019,9(19):10554
Mercury (Hg) is one of the major toxic heavy metals, harmful to the environment and human health. Thus, it is significantly important to find an easy and quick method to detect Hg2+. In this study, we designed and synthesized a simple fluorescent probe with excellent properties, such as high sensitivity and selectivity, rapid response, and outstanding water solubility. When Hg2+ (5 μM) was added to the probe solution, it exhibited a very large fluorescent enhancement (about 350-fold stronger than the free probe) with the help of hydrogen peroxide (H2O2). Probe HCDC could quantitatively detect Hg2+ in the range of 0–10 μM using the fluorescence spectroscopy method and the detection limit was measured to be about 0.3 nM (based on a 3σ/slope). Analytical application was also studied, and the probe HCDC exhibited excellent response to Hg2+ with the addition of H2O2 in real water samples. So, our proposed probe HCDC provided a practical and promising method for determining Hg2+ in the environment.A novel water-soluble, highly selective and sensitive rapid-response fluorescent probe was developed to monitor mercury ions in real water samples. 相似文献
14.
Junwei Chen Yuna Lin Wanjin Xing Xingchen Zhang Huan Xu Wei Wang Kaiyan Lou 《RSC advances》2022,12(15):9473
An anthracenecarboximide-guanidine based turn-on fluorescent probe ANC-DCP-1 for selective detection of glyoxals (methylglyoxal and glyoxal, GOS) over formaldehyde under weak acidic conditions around pH 6.0 was reported. The probe showed great potential in studying relative GOS levels in weak acidic biological fluids such as in urine for diabetic diagnosis and prognosis, and also found application in the food industry such as for fast unique manuka factor (UMF) scale determination of Manuka honey.Formation of 5-membered dihydroxyimidazolidines with increased deprotonation at around pH 6.0 and enhanced intramolecular charge transfer for turn-on fluorescence detection of glyoxals. 相似文献
15.
16.
Lihong Li Min Zhang Kaijing Chang Yu Kang Guodong Ren Xiaoyu Hou Wen Liu Haojiang Wang Bin Wang Haipeng Diao 《RSC advances》2019,9(55):32308
A highly sensitive and selective fluorescent probe for fluoride ions has been developed by incorporating the dimethylphosphinothionyl group as a recognition moiety into the fluorophore of coumarin. The detection mechanism is based on the fluoride ion-triggered cleavage of the dimethylphosphinothionyl group, followed by the release of coumarin, which leads to a large fluorescence enhancement at 455 nm (λex = 385 nm). Under the optimized conditions, the fluorescence enhancement of the probe is directly proportional to the concentration of fluoride ions in the range of 0–30 μM with a detection limit of 0.29 μM, which is much lower than the maximum content of fluoride ions guided by WHO. Notably, satisfying results have been obtained by utilizing the probe to determine fluoride ions in real-water samples and commercially available toothpaste samples. The proposed probe is rather simple and may be useful in the detection of fluoride ions in more real samples.A sensitive and selective fluorescent off–on probe is developed for fluoride ion detection, and its applicability has been demonstrated by determining fluoride ions in real-water samples and toothpaste samples. 相似文献
17.
Gengo Kashiwazaki Ryo Watanabe Akihiro Nishikawa Koyori Kawamura Takashi Kitayama Takao Hibi 《RSC advances》2022,12(24):15083
Fructose is widely used in the food industry. However, it may be involved in diseases by generating harmful advanced glycation end-products. We have designed and synthesized a novel fluorescent probe for fructose detection by combining a phenylboronic acid group with a BODIPY-based hydrophobicity probe. This probe showed a linear fluorescence response to d-fructose concentration in the range of 100–1000 μM, with a detection limit of 32 μM, which is advantageous for the simple and sensitive determination of fructose.We have designed and synthesized a novel fluorescent probe for fructose detection through hydrophobic interactions by combining a phenylboronic acid group and a BODIPY-based hydrophobicity probe with a detection limit of 32 μM. 相似文献
18.
Based on the fluorescence quenching phenomenon, a smart fluorescent microsensor was synthesized. The bifenthrin (BI) microsensor inherited the high selectivity of molecular imprinted polymers (MIPs) and the excellent fluorescence properties of aqueous CdTe quantum dots (QDs). Aqueous CdTe QDs are functionalized by octadecyl-4-vinylbenzyl-dimethyl-ammonium chloride (OVDAC). A type of functional monomer, 4-vinylphenylboronic acid (VPBA), was used and its boronic acid groups could covalently combine with a cis-diol compound for direct imprinting polymerization. The OVDAC-functionalized aqueous CdTe QDs were used as solid supports and auxiliary monomers. Under optimal conditions, experimentation showed that BI had a linear detection range of 10 to 300 μmol L−1 with a correlation coefficient of 0.9968 and a high imprinting factor (IF) of 4.53. In addition, the prepared MIP-OVDAC/CdTe QDs were successfully used to detect BI in water samples. Therefore, this work provided a highly selective and sensitive fluorescence probe for the detection of BI. In addition, the fluorescence probe could be used to detect other targets by changing the functional monomers.Based on the fluorescence quenching phenomenon, a smart fluorescent microsensor was synthesized. 相似文献
19.
A new, robust and reliable methodology for three-protease screening in a single-enzyme mode has been developed and verified, employing a multi-purpose peptide probe with three selectively cleavable sites furnished with four fluorophores. A triple-FRET-based single-excitation quadruple-emission concept for unambiguous sensing of trypsin, chymotrypsin and caspase-8 in the lowest detectable concentrations of 0.5 ng mL−1, 0.2 μg mL−1, and 2 U mL−1, respectively, has been applied and graphically depicted. Then the developed 4-dye probe has been also studied from the perspective of simultaneous two-protease screening, which was found only partially feasible, primarily due to unselective chymotrypsin cleavage.A triple-FRET four-dye system for detection of three proteases has been developed and verified. 相似文献
20.
Mingxin Luo Bo Sun Chen Zhou Qingqing Pan Yue Hou Huan Zhang Jing Sun Chenyang Zou 《RSC advances》2022,12(36):23215
In this research, a novel selective and sensitive fluorescent sensor for detecting Fe3+ was designed and synthesized; it revealed an obvious fluorescence quenching effect upon addition of Fe3+, and possessed the quantitative analysis ability on account of the formation of a 1 : 1 metal–ligand complex. Furthermore, the density functional theory calculations were utilized to study the molecular orbitals as well as the spatial structure. Simultaneously, the cell experiments and zebra fish experiments verified the application value of the sensor in the biological field.A novel selective and sensitive fluorescent sensor for detecting Fe3+ was designed and synthesized; it revealed obvious fluorescence quenching effect upon addition of Fe3+, and possessed the quantitative analysis ability on account of the formation of a 1 : 1 metal–ligand complex. 相似文献