复方延胡索片中延胡索的醇提工艺优选

复方延胡索片系根据妇科名老中医经验方研制成的复方制剂，由延胡索、蒲黄、三棱等8味中药组成，具活血化瘀，破气行滞，消积定痛之功效，用于治疗妇女原发性痛经。延胡索为方中君药，所含化学成分主要为生物碱，如延胡索甲素(延胡索碱，d-cordaline)、乙素(dl-四氢掌叶防己碱，dz-     

延胡索乙素提取工艺的改进

<正> 延胡索乙素(Corydalis B)为有效的镇静、止痛药。我们以防己科植物黄藤(Fibraurea recisa Pierre)为原料,首先提取掌叶防己碱,经氢化反应,制取延胡索乙素结晶,并对原提取方法作了改进,收到较好的效果。一、工艺流程     

延胡索炮制前后多组分质量控制方法的研究

[目的]建立超高效液相色谱法(UPLC)同时测定延胡索药材中四氢非洲防己碱、原阿片碱、四氢黄连碱、非洲防己碱、延胡索乙素、盐酸小檗碱、盐酸巴马汀、去氢紫堇碱8种生物碱含量的方法,并对延胡索生品及炮制品进行测定。[方法]采用UPLC检测方法,ACQUITY UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm),乙腈(B)-0.2%甲酸水溶液,梯度洗脱,流速:0.3 mL/min,柱温55℃,检测波长280 nm。[结果]四氢非洲防己碱,原阿片碱、四氢黄连碱、非洲防己碱、延胡索乙素、盐酸小檗碱、盐酸巴马汀、去氢紫堇碱在0.395～98.8、0.4～100.2、0.99～247.5、0.197～49.4、1.004～251、0.198～49.5、0.398～99.6、1～250μg/mL浓度范围内线性良好,平均加样回收率在98.10%～100.87%,RSD≤1.94(n=6)。[结论] UPLC所建立的方法简便、快速、准确,可应用于延胡索药材中多种成分含量测定,为延胡索药材质量控制提供更加科学、便捷的方法。     

黄芪、当归及其配伍对正常和血虚模型大鼠全血比粘度和血浆比粘度的影响

反相ＨＰＬＣ法测定３种延胡索生物碱含量祝晨陈，何宏贤，金蓉鸾。中国中药杂志，１９９４，１９（２）：７７～７９采用反相ＨＰＬＣ法分析了灰绿延胡素、长距延胡索和对叶延胡索中８种生物碱（延胡索丙素，巴马亭，去氢紫堇碱，延胡索乙素，延胡索甲素，黄连碱，去氢紫...     

D-四氢掌叶防己碱通过AMPK信号通路抑制HepG2肝癌细胞脂肪蓄积的作用及其机制

[目的]探讨D-四氢掌叶防己碱在HepG2肝癌细胞中对脂肪蓄积的抑制作用及相关机制．[方法]用D-四氢掌叶防己碱（0,5,10,20,40,80umo／L）处理HepG2肝癌细胞24h,采用MTS法测定细咆毒性;采用Westernblot方法检测与脂肪积蓄有关的AMPK蛋白质的表达情况;采用RT-PCR方法检测与AMPK信号通路有关的SREBP1c、脂肪酸舍酶（FAS）及硬脂酰辅酶去饱和酶（SCDl）等相关基因的表达情况．[结果]D-四氢掌叶防己碱呈浓度依赖性地抑制三酰甘油的含量,增加AMPK磷酸化;D-四氢掌叶防己碱抑制与脂肪合成有关的SREBPlc、FAS及SCDl基因的表达．[结论]D-四氢掌叶防己碱在HepG2肝癌细咆中通过AMPK信号通路抑制脂肪蓄积．     

黑木耳多糖的分离、纯化和鉴定

延胡索中的季铵碱(如去氢延胡索甲素)在缺氧条件下能明显延长动物的存活时间。作者采用分光光度法测定了延胡索生品及醋炒、酒炒和盐炒三种延胡索炮制品中的季铵盐含量,并以心得安为阳性对照     

HPLC同时测定复方防己注射液中延胡索乙素、粉防己碱和防己诺林的含量

目的:建立同时测定复方防己注射液中延胡索乙素、粉防己碱和防己诺林的含量的方法。方法:色谱条件Diamonsil C_(18)(4.6 mm×150 mm,5μm),流动相为乙腈-0.2%三乙胺梯度洗脱,检测波长为280 nm。结果:延胡索乙素、粉防己碱和防己诺林碱线性范围分别为0.5556~4.630μg,8.772~73.10μg和5.133~42.775μg(r=0.9999)。方法回收率分别为98.9%,102.5%,100.6%(RSD2%)。结论:本方法准确,简便,可为复方防己注射液中延胡索乙素、粉防己碱和防己诺林的含量测定方法提供依据。     

延胡索中生物碱成分的研究

目的 提取分离延胡索中的生物碱供延胡索种质评价使用。方法 运用色谱学和波谱学方法分离鉴定延胡索中的生物碱。结果 从延胡索总碱中分离得到了13种生物碱，鉴定了其中12种生物碱的结构，分别为:d-紫堇碱，四氢巴马亭，脱氢紫堇碱，四氢黄连碱，四氢小檗碱，异紫堇球碱，原托品碱，四氢非洲防己胺，氯仿巴马亭，saulatine，海罂粟碱，脱氢海罂粟碱。结论 生物碱saulatine为首次从罂粟科植物中分得，异紫堇球碱为首次从延胡索中分得。     

中药黄藤中的季铵生物碱研究

本文从中药黄藤的根及根茎中分得三种季铵生物碱,化学及光谱数据证明,一为掌叶防己碱(巴马汀),二为药根碱,三系伪非洲防己胺碱。后者为国内首次得到的一种天然伪原小檗碱型生物碱。     

汉防己甲素脂质体在大鼠体内的药动学研究

目的 考察汉防己甲素脂质体在大鼠体内的药动学特性.方法 采用薄膜分散法制备汉防己甲素脂质体,大鼠尾静脉分别注射汉防己甲素脂质体或溶液剂,用HPLC测定不同时间血浆中汉防己甲素的浓度,通过3P97程序计算药动学参数.结果 制得的脂质体包封率为(79.7±0.68)%,汉防己甲素脂质体和溶液剂大鼠尾静脉单次给药均符合二室开放模型,与溶液剂相比,汉防己甲素脂质体的AUC显著增加.结论 汉防己甲素脂质体能显著增加汉防己甲素在大鼠体内的AUC,延长其在血液循环中的驻留时间.     

脱氢紫堇碱体外对兔血小板中环核苷酸含量的影响

目的：研究脱氢紫堇碱（DHC）对兔血小板中cAMP和cGMP含量的影响以探讨其抑制血小板聚集的作用机制。方法：应用放射免疫分析方法。结果：DHC显著增加兔血小板中cAWP的含量，而降低兔血小板中cGMP的含量。结论：DHC可能通过增加血小板中cAMP的含量和降低血小板中cGMP的含量而产生血小板抑制作用。     

中药延胡索类研究——Ⅰ.4种延胡索块茎中生物碱的含量比较

本文用反相高效液相色谱法分离测定了延胡索Corydalis yanhusuo W.T.Wang、齿瓣延胡索C.turtschaninovii Bess.、东北延胡索C.ambigua Cham.et Schltd.、土元胡C.humosa Migo及2种野生延胡索C.yanhusuo块茎,5省市的11件栽培品,3件商品中叔胺型生物碱(±)-四氢巴马亭、(+)-紫堇碱和季铵型生物碱黄连碱、巴马亭、去氢紫堇碱的含量。     

延胡索生熟饮片在复方配伍后药效成分溶出规律研究

[目的]研究延胡索生熟饮片在不同复方中配伍后生物碱类成分的溶出规律。[方法]以金铃子散、元胡止痛片和延胡索汤的组成为依据,将延胡索和醋延胡索分别入3个复方中,采用超高液相色谱法(UPLC)对3个复方配伍后的水提液进行检测,观察生物碱类成分的溶出规律。[结果]无论选用延胡索还是醋延胡索,3个复方配伍组合与未配伍者相比,延胡索乙素溶出量均未显著提高,去氢紫堇碱也呈现相同的规律。[结论]延胡索生熟饮片在这3个复方配伍组合后对其延胡索乙素和去氢紫堇碱溶出量的影响差异较小。     

Effects of Postharvest Processing and Geographical Source on Phytochemical Variation of Corydalis Rhizoma

Objective To assess the relative contributions of postharvest processing and geographical source to phytochemical variation of Corydalis Rhizoma, and rhizome of Corydalis yanhusuo, and to examine what phytochemical components are the most sensitive to the differences of each factor and how they change. Methods HPLC fingerprinting and LC-MS coupled with chemometric approaches were applied. Results The results of principal component analysis (PCA) and hierarchical cluster analysis (HCA) explicitly demonstrated the postharvest processing could produce a greater impact on the phytochemical profiles of Corydalis Rhizoma than geographical source. The contents of most compounds increased after water boiling while decreased after sulphur-fumigation. Protopine, coptisine, and palmatine were the most variable components in processing. Geographical sources also led to a remarkable phytochemical differentiation, in which the environmental variation of the three regions might play a role. Dehydrocorybulbine, coptisine, dehydrocorydaline, and protopine varied most among the three production regions and decreased sequentially in Zhejiang, Shaanxi, and Jiangsu provinces, China. Conclusion Both postharvest processing and geographical source should be enhanced with the priority for the former in the quality control of Corydalis Rhizoma. The application of boiling is supported but the consistency should be improved in practice. Sulphur-fumigation is strongly suggested to be abandoned.     

速效感冒片中扑热息痛的溶出度研究

速效感冒片中扑热息痛的溶出度测定方法，采用双波长分光光度法测定扑热息痛的溶出量，方法回收率（ｎ＝５）为９９．７９％，ＣＶ为０．０７２％，该法操作简便、准确度和灵敏度高。所拟的溶出度测定方法可做为质量评价指标。     

应用ACT—Ultiogel ACA22新和层析柱分离纯化甲胎蛋白

In the present communication, we report a method for the isolation and purification of alpha FP from fetal corpse homogenate in a single-step procedure using Act-Ultrogel AcA 22 affinity chromatogrpahy column. This method proves to be better than the conventional method in simplicity, fastness and validity with recovery, purity and immunoreactivity. The immunoreactivity of the purified alpha FP labelled by the method of Hunter and Greenwood (1962) was 50-60% (B/T%). This was similar to alpha FP standard (WHO). The results indicate that the 125I-alpha FP is suitable for alpha FP radioimmunoassay.     

非同位素PCR方法检测脆性X 综合征FMR-1基因CGG重复序列数目

目的 建立一种可靠、简便的非同位素PCR方法检测脆性X综合征的突变基因。方法 采用生物素标记的CGG寡核苷酸探针，检测通过PCR扩增的FMR-1基因中CGG三核苷酸重复序列数目。从而判断所检样品中FMR—1基因是否正常。结果 该法可检测出正常人及携带者的CGG重复拷贝数。结论 该方法可以简便、安全、可靠地检测FMR—1基因中(CGG)n重复拷贝数，从而确定为正常人或携带者，可作为临床上筛查脆性X综合征的首选方法。     

氧瓶燃烧法测定诺氟沙星胶囊的含量

采用氧瓶燃烧法测定诺氟沙星胶囊的含量，并与非水法、紫外分光光度法进行了比较，结果表明氧瓶燃烧法定量准确，灵敏度高，平均回收率为１０１．３％，ＲＳＤ＝０．５５％（ｎ＝３），可作为诺氧沙星含量测定的一种新方法。     

An Objective Evaluation Method Designed for Pulsating Cardiac Phantom with 64-row MDCT

In the research of non-invasive cardiac coronary imaging with multi-detector CT (MDCT), it is necessary to use a standard cardiac phantom to perform the mass repetitive experiments. The standard phantom can help to investigate the regularity of a technology, and hence provide the instructions to clinic. In this study, an objective evaluation method of coronary stenosis rate, which can process CT images automatically, is developed with a cardiac phantom, to enhance working efficiency and to reduce subjective error. Several experiments were designed to verify the accuracy, the sensitivity and the consistency of this method. Based on the results, we found the accuracy of this objective method is within the clinical acceptable range. This method is sensitive to image quality change, and the results of the method are consistent without being affected by the environment and the operators. This proposed method can help to improve the efficiency of processing mass experimental data based on the cardiac phantom and establish a good foundation for the research on the cardiac coronary imaging with MDCT. This method is also applied to clinical images for tests and acceptable results are achieved in stenosis of low density.