Biocompatible supramolecular pseudorotaxane hydrogels for controllable release of doxorubicin in ovarian cancer SKOV-3 cells

A series of injectable and biocompatible delivery DOX-loaded supramolecular hydrogels were fabricated by using presynthesized DOX-2N-β-CD, Pluronic F-127 and α-CD through host–guest interactions and cooperative multivalent hydrogen bonding interactions. The compositions and morphologies of these hydrogels were confirmed by PXRD and SEM measurements. Moreover, the Rheological measurements of these hydrogels were studied and the studies found that they showed a unique thixotropic behavior, indicting a fast self-healing property after the continuous oscillatory shear stress. Using α-CD as a capping agent, slow and sustained DOX release was observed at different pH values after 72 h. The amount of DOX released at pH 7.4 was determined to be 49.0% for hydrogel 1, whereas the releasing amount of the DOX was increased to 66.3% for hydrogel 1 during the same period at pH 5.5 (P < 0.05), indicating a higher release rate of the drug under more acidic conditions. Taking hydrogel 1 as a representative material, the toxicities of DOX and hydrogel 1 on ovarian cancer cells (SKOV-3) at different exposure durations were examined. The results revealed that hydrogel 1 was less cytotoxic than free DOX to SKOV-3 cells (P < 0.05), suggesting sustained release by these hydrogels in the presence of ovarian cancer cells. It is anticipated that this exploration can provide a new strategy for preparing drug delivery systems.

A series of injectable and biocompatible delivery DOX-loaded supramolecular hydrogels were fabricated by using presynthesized DOX-2N-β-CD, Pluronic F-127 and α-CD through host–guest interactions and cooperative multivalent hydrogen bonding interactions.     

A graphene hybrid supramolecular hydrogel with high stretchability,self-healable and photothermally responsive properties for wound healing

Wound healing is a ubiquitous healthcare problem in clinical wound management. In this paper, the fabrication of a graphene hybrid supramolecular hydrogel (GS hydrogel) for wound dressing applications is demonstrated. The hydrogel is composed of two components, including N-acryloyl glycinamide (NAGA) as the scaffold and graphene as the photothermally responsive active site for photothermal therapy. Based on the multiple hydrogen bonds between the dual amide motifs in the side chain of N-acryloyl glycinamide, the hydrogel exhibits high tensile strength (≈1.7 MPa), good stretchability (≈400%) and self-recoverability. In addition, the GS hydrogel shows excellent antibacterial activity towards methicillin-resistant Staphylococcus aureus (MRSA), benefiting from the addition of graphene that possesses great photothermal transition activity (≈85%). Significantly, in vivo animal experiments also demonstrated that the GS hydrogel effectively accelerates the wound healing processes by eradicating microbes, promoting collagen deposition and angiogenesis. In summary, this GS hydrogel demonstrates excellent mechanical performance, photothermal antimicrobial activity, and promotes skin tissue regeneration, and so has great application potential as a promising wound dressing material in clinical use.

The hydrogel demonstrated properties with high stretchability, self-healable and photothermal properties. Notably, photothermal therapy could be established due to its photothermal responsiveness, benefiting infected wound healing.     

A pH-sensitive and sustained-release oral drug delivery system: the synthesis,characterization, adsorption and release of the xanthan gum-graft-poly(acrylic acid)/GO–DCFP composite hydrogel

In this study, graphene oxide (GO) was successfully prepared using the improved Hummers method, and the prepared GO powder was dissolved in distilled water and subjected to ultrasonic stripping. Diclofenac potassium (DCFP) was selected as a model drug to systematically evaluate the adsorption mechanism of DCFP by GO. Different reaction models were constructed to fit the adsorption kinetics and adsorption isotherms of DCFP on GO, in order to further explore the underlying adsorption mechanism. The results demonstrated that the pseudo-second-order kinetic model and Freundlich model could better delineate the adsorption process of DCFP by GO. Both π–π stacking and hydrophobic interaction were mainly involved in the adsorption process, and there were electrostatic interaction and hydrogen bonding at the same time. Then, the xanthan gum-graft-poly(acrylic acid)/GO (XG-g-PAA/GO) composite hydrogel was synthesized by in situ polymerization as a slow-release drug carrier. For this reason, a XG-g-PAA/GO–DCFP composite hydrogel was synthesized, and its in vitro drug release and pharmacokinetic data were assessed. The results showed that the synthesized XG-g-PAA/GO composite hydrogel had a certain mechanical strength and uniform color, indicating that GO is evenly distributed in this composite hydrogel. Moreover, the results of a swelling ratio test demonstrated that the swelling ratios of the XG-g-PAA/GO composite hydrogel were significantly increased with increasing pH values, implying that this material is sensitive to pH. The in vitro drug release experiment showed that the cumulative release of DCFP after 96 h was significantly higher in artificial intestinal fluid than in artificial gastric fluid. These findings indicate that the XG-g-PAA/GO–DCFP composite hydrogel exhibits pH sensitivity under physiological conditions. Besides, the results of in vivo pharmacokinetic analysis revealed that the t_1/2 of DCFP group was 2.03 ± 0.35 h, while that of the XG-g-PAA/GO–DCFP composite hydrogel group was 10.71 ± 2.04 h, indicating that the synthesized hydrogel could effectively prolong the drug action time. Furthermore, the AUC_(0–t) of the DCFP group was 53.99 ± 3.18 mg L⁻¹ h⁻¹, while that of the XG-g-PAA/GO–DCFP composite hydrogel group was 116.79 ± 14.72 mg L⁻¹ h⁻¹, suggesting that the bioavailability of DCFP is greatly enhanced by this composite hydrogel. In conclusion, this study highlights that the XG-g-PAA/GO–DCFP composite hydrogel can be applied as a sustained-release drug carrier.

In this study, graphene oxide (GO) was successfully prepared using the improved Hummers method, and the prepared GO powder was dissolved in distilled water and subjected to ultrasonic stripping.     

NIR laser-responsive liquid metal-loaded polymeric hydrogels for controlled release of doxorubicin

Liquid metals (LMs) have recently emerged as a new class of promising multifunctional materials with attractive properties. They have excellent photothermal conversion efficiency, generating heat under near-infrared (NIR) laser irradiation. This work reports encapsulating LM droplets into poly(NIPAm-co-MBA) hydrogels (PNM) to achieve nanodispersed liquid metals in bulk polymeric hydrogels for NIR laser-responsive materials. LM droplets (∼530 nm) are produced by dispersing an alloy of gallium and indium (EGaIn) into glycerol. The LM-loaded PNM hydrogels (PNM/LM) exhibited excellent thermal-/NIR laser-responsive ability. In a water bath, the weight of the PNM/LM can decrease 92% at 50 °C. And the volume of PNM/LM can decrease 62% under NIR laser irradiation for 12 min. Because of its thermal-/NIR laser-responsive ability and porous three-dimensional (3D) networks, PNM/LM is very suitable for use as a drug carrier. We also prepared doxorubicin (DOX)-loaded PNM/LM hydrogels (PNM/LM/DOX) and demonstrated that the PNM/LM/DOX hydrogel can generate heat and raise its temperature under NIR laser irradiation. When the temperature becomes higher than the lower critical solution temperature (LCST), such a hydrogel would shrink immediately and extrude the DOX encapsulated in its networks simultaneously, then complete the controlled release of the pre-loaded drug. Further, an in vitro cytotoxicity test indicated the biocompatibility and feasibility as a chemophotothermal synergistic therapeutic of the present hydrogel. This NIR laser-responsive hydrogel fully exhibits its superiority as a drug carrier which promises great potential in future targeted controlled drug release.

NIR laser-responsive liquid metal-loaded polymeric hydrogel exhibits its superiority in drug carrier of controlled release.     

Anisotropic tough poly(vinyl alcohol)/graphene oxide nanocomposite hydrogels for potential biomedical applications

Hydrogels, one of the most important bioinspired materials, are receiving increasing attention because of their potential applications as scaffolds for artificial tissue engineering and vehicles for drug delivery, etc. However, these applications are always severely limited by their microstructure and mechanical behavior. Here we report the fabrication of a tough polyvinyl alcohol/graphene oxide (PVA/GO) nanocomposite hydrogel through a simple and effective directional freezing–thawing (DFT) technique. The resulting hydrogels show well-developed anisotropic microstructure and excellent mechanical properties with the assistance of DFT method and lamellar graphene. The hydrogels with anisotropic porous structures that consisted of micro-sized fibers and lamellas exhibit high tensile strengths, up to 1.85 MPa with a water content of 90%. More interestingly, the PVA/GO composite hydrogels exhibit the better thermostability, which can maintain the original shape when swollen in hot water (65 °C). In addition, the hydrogels with biocompatibility show good drug release efficiency due to the unique hierarchical structure. The successful synthesis of such hydrogel materials might pave the way to explore applications in biomedical and soft robotics fields.

Tough PVA/GO nanocomposite hydrogel with well-developed anisotropic microstructure and excellent mechanical properties.     

Dual responsive oligo(lysine)-modified Pluronic F127 hydrogels for drug release of 5-fluorouracil

Peptide-containing hydrogels have become a research hotspot due to their unique secondary structure and biocompatibility. Herein, we used amino-terminated F127 as a macroinitiator to initiate the ring-opening polymerization of l-lysine(z)-NCA, and the obtained oligo(lysine)-modified F127 (FL) had degrees of polymerization of lysine of 2, 5, and 8. The results showed that the FL hydrogels had reversible temperature-dependent sol–gel transitions, and the introduction of lysine increased the critical gel temperature. In the dilute solution of FL, the micelle size increased and aggregated as the pH increased; the micelle grew into a rod-like shape under alkaline conditions. Scanning electron micrographs showed that the interior of the FL hydrogel had a more complete porous structure. The FL-2 hydrogel loaded with 5-fluorouracil exhibited an approximately linear release trend within 12 h and has good biocompatibility. Therefore, FL hydrogels have potential applications in the field of biomedicine.

Oligo(lysine)-F127 hydrogels have a temperature-responsive sol–gel transition and pH-responsive micelle morphology.     

Mussel-inspired in situ fabrication of a photothermal composite hydrogel for MR-guided localized tumor ablation

Photothermal ablation could be considered an effective treatment for tumors, but accurate administration and enrichment of photothermal agents remain a huge challenge. Herein, a mussel-inspired photothermal polymeric hydrogel (PPH) was synthesized through a ferric iron-triggered simultaneous metal–catechol coordination reaction and oxidative polymerization of covalently linked pyrrole. The PPH with rapid gelation (less than 10 s) exhibited high photothermal conversion efficiency (49.3%), which enabled effective hyperthermal therapy in situ. Besides, the introduced iron could be used as a T₂-weighted contrast agent for real-time MR imaging to explore the retention and bio-degradation of PPH in vivo. Overall, our findings evidence that the resultant PPH, which possesses potential application in tumor ablation in situ, and metal–catechol coordination strategy inspired by mussel adhesion may stimulate biomedical hydrogel development.

A mussel-inspired photothermal polymeric hydrogel (PPH) was synthesized has been successfully used in tumor ablation in situ.     

Tetracycline hydrochloride loaded citric acid functionalized chitosan hydrogel for wound healing

Citric modified chitosan (CC) hydrogel containing antibacterial drugs is developed by the freezing and thawing treatment method. The SEM image of the CC hydrogel revealed a porous structure. The rheological properties, porosity, swelling rate, water uptake, tensile properties and in vitro degradation were found to be tunable via CC concentration. To enhance antibacterial properties, tetracycline hydrochloride (TH) representing the drug model, was integrated into the CC hydrogel. The cumulative release of drug was also tunable via CC concentration. The drug loaded CC hydrogel showed enhanced antimicrobial activity against E. coli and S. aureus. In animal tests, it was found the TH loaded CC hydrogel accelerated the healing of the wounds created on rats. These results suggest that the drug loaded CC hydrogel has a promising future in wound healing as a wound dressing.

Citric modified chitosan (CC) hydrogel containing antibacterial drug for wound healing applications.     

In situ synthesis of ZnO–GO/CGH composites for visible light photocatalytic degradation of methylene blue

A novel ZnO–GO/CGH composite was prepared using an in situ synthesis process for photodegradation of methylene blue under visible light illumination. The chitin–graphene composite hydrogel (CGH) was used to provide uniform binding of the nano ZnO–GO composite to the hydrogel surface and prevent their agglomeration. GO provides multi-dimensional protons and electron transport channels for ZnO with a flower-like structure, which possessed improved photo-catalytic activity. SEM analysis indicates that the hydrogel has good adsorption properties with rougher surfaces and porous microstructure, which enables it to adsorb the dyes effectively. Under synergetic enhancement of adsorption and photo-catalysis, catalytic activity and nano ZnO–GO/CGH recycling improved greatly. Synthesized nano ZnO–GO/CGH showed high dye removal efficiency of 99%, about 2.2 times that of the pure chitin gel under the same condition. This suggests the potential application of the new photocatalytic composites to remove organic dyes from wastewater.

A novel ZnO–GO/CGH composite was prepared using an in situ synthesis process for photodegradation of methylene blue under visible light illumination.     

UCN–SiO2–GO: a core shell and conjugate system for controlling delivery of doxorubicin by 980 nm NIR pulse

Herein, graphene oxide (GO) has been attached with core–shell upconversion-silica (UCN–SiO₂) nanoparticles (NPs) to form a GO–UCN–SiO₂ hybrid nanocomposite and used for controlled drug delivery. The formation of the nanocomposite has been confirmed by various characterization techniques. To date, a number of reports are available on GO and its drug delivery applications, however, the synergic properties that arise due to the combination of GO, UCNPs and SiO₂ can be used for controlled drug delivery. New composite UCN@SiO₂–GO has been synthesized through a bio-conjugation approach and used for drug delivery applications to counter the lack of quantum efficiency of the upconversion process and control sustained release. A model anticancer drug (doxorubicin, DOX) has been loaded to UCNPs, UCN@SiO₂ NPs and the UCN@SiO₂–GO nanocomposite. The photosensitive release of DOX from the UCN@SiO₂–GO nanocomposite has been studied with 980 nm NIR laser excitation and the results obtained for UCNPs and UCN@SiO₂ NPs compared. It is revealed that the increase in the NIR laser irradiation time from 1 s to 30 s leads to an increase in the amount of DOX release in a controlled manner. In vitro studies using model cancer cell lines have been performed to check the effectiveness of our materials for controlled drug delivery and therapeutic applications. Obtained results showed that the designed UCN@SiO₂–GO nanocomposite can be used for controlled delivery based therapeutic applications and for cancer treatment.

A GO–UCN–SiO₂ hybrid nanocomposite for loading of doxorubicin and its use in in vitro efficiency for killing carcinoma cells.     

Silver ions blocking crystallization of guanosine-based hydrogel for potential antimicrobial applications

In this work, the detailed crystallization process of 2′-deoxy-2′-fluoroguanosine (^FG_d) hydrogel has been studied using single crystal X-ray diffraction, variable-temperature nuclear magnetic resonance (VT-NMR), and scanning electron microscopy (SEM). Both solid and solution results indicated that the K⁺-mediated G-quartet structures were unstable and easily resulted in the breakdown of the hydrogel to form linear ribbon structures by forming mimic reverse Watson–Crick base pairs between the two faces with an intermolecular hydrogen-bond (N10H–O11). Accordingly, Ag⁺ was introduced to block the crystallization of ^FG_d to form long lifetime stable supramolecular hydrogel (>6 months) and possible silver-ions-mediated base pair motifs were suggested via NMR, UV, and mass spectroscopy (MS) in combination with powder X-ray diffraction (PXRD) and circular dichroism spectroscopy (CD). Furthermore, ^FG_dAg hydrogel exhibited low toxicity for normal oral keratinocyte cells (NOK-SI) and good antibacterial activities for Fusobacterium nucleatum in vitro.

Ag⁺ was introduced to block the crystallization of ^FG_d forming long lifetime stability supramolecular hydrogel (>6 months), which displayed low toxicity for NOK-SI cell and good antibacterial activities.     

Novel albumin-binding photothermal agent ICG-IBA-RGD for targeted fluorescent imaging and photothermal therapy of cancer

In this work, we present a novel photothermal agent ICG-IBA-RGD based on albumin-binding strategy for enhanced tumor targeting imaging and photothermal therapy. In vitro and in vivo experiments demonstrated that ICG-IBA-RGD exhibits excellent photothermal conversion capability and high tumor ablation efficiency.

In this work, we present a novel photothermal agent ICG-IBA-RGD based on albumin-binding strategy for enhanced tumor targeting imaging and photothermal therapy.     

Effective photocatalytic degradation and physical adsorption of methylene blue using cellulose/GO/TiO2 hydrogels

Environmentally friendly cellulose/GO/TiO₂ hydrogel photocatalyst has been successfully fabricated via a green, simple, and one-step method and evaluated as the photocatalyst and adsorbent for the removal of methylene blue (MB). The XRD and FTIR analysis suggested the strong interaction among cellulose, GO and TiO₂, resulting from the formation of hydrogen bonds. Due to the unique porous structure of cellulose hydrogel and introduction of GO, the cellulose/GO/TiO₂ hydrogel showed superior (degradation ratio ∼ 93%) and reproducible (no significant change during the ten consecutive cycles) performance in the removal of MB under UV light. Consequently, the prepared cellulose/GO/TiO₂ hydrogel can be applied as an eco-friendly, high-performance, reproducible, and stable photocatalyst and adsorbent for the removal of MB. This green hydrogel is a promising candidate for dye wastewater treatment. Moreover, this work is expected to extend the scope of bio-templated synthesis of other nanomaterials for various applications.

New functional cellulose/GO/TiO₂ hydrogels are prepared via a simple method, showing superior and reproducible performance in the removal of MB.     

Dissolving microneedles delivering cancer cell membrane coated nanoparticles for cancer immunotherapy

Recently, a variety of tumor vaccines and immune system stimulators such as toll-like receptor (TLR) agonists have been widely investigated for cancer immunotherapy via transdermal delivery. Despite these great research efforts, low efficiency and discomfort remain a huge technical hurdle for the development of immunotherapeutics. Here, we design a facile method to deliver drugs to the skin through microneedles (MNs) to stimulate the immune system in two ways. As one of the tumor vaccines, cancer cell membrane proteins can act as tumor-specific antigens that are presented to antigen presenting cells (APCs) to activate the immune system. In addition, a toll-like receptor 7 (TLR7) agonist of imiquimod (R837) can suppress cancer cell growth by inhibiting angiogenesis. Using poloxamer 407 (F127) as a nanocarrier, F127 nanoparticles (F127 NPs) are loaded with R837 and then coated with cancer cell membranes (M). These F127–R837@M NPs are loaded in rapidly dissolving MNs and delivered through the skin. MNs loaded with F127–R837@M NPs show significant inhibition of cancer cell growth in both prophylactic vaccination and antitumor immunotherapy in vivo. The dual immune system stimulating F127–R837@M NPs could be effectively used for cancer immunotherapy.

Recently, a variety of tumor vaccines and immune system stimulators such as toll-like receptor (TLR) agonists have been widely investigated for cancer immunotherapy via transdermal delivery.     

Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids

Hydrogels with concave microwells are one of the simplest means to obtain uniform-sized cellular spheroids. However, the inherent swelling of hydrogels leads to reduced mechanical strength and thus deforms the structure of the microwells. In this study, we developed a hydrogel with microwells for formation of vascular spheroids via non-swellable di-acrylated Pluronic F127 (F127-DA), which showed higher mechanical strength than a conventional di-acrylated polyethylene glycol (PEG-DA) hydrogel. The uniform-sized vascular spheroids were spontaneously generated by human umbilical vein endothelial cells (HUVECs) and fibroblasts in the microwells. The endothelial functions of vascular spheroids were about 1-fold higher than those in two-dimensional (2D) culture, as indicated by secretion of nitric oxide (NO), prostacyclin (PGI2) and tissue factor pathway inhibitor (TFPI). Interestingly, the vascular spheroids with large diameter showed higher sensitivity to ethanol toxicity than those with small diameter, possibly due to the higher endothelial functions of large spheroids. Hence, F127-DA hydrogel with concave microwells provides a convenient way of forming uniform-sized spheroids that are useful for high throughput screening of drug/food toxicity.

Hydrogels with concave microwells are one of the simplest means to obtain uniform-sized cellular spheroids.     

Ultra-high thermally stable gold nanorods/radial mesoporous silica and their application in enhanced chemo-photothermal therapy

In this work, gold nanorods embedded in ultra-thick silica shells with radial mesopores (AuNR/R-SiO₂) were successfully synthesized in an ethanol/water solution. By optimizing the concentration of CTAB and the volume of ethanol, a shell thickness up to 83 nm was realized. Taking advantage of the ultra-thick silica shell, AuNR/R-SiO₂ exhibited ultra-high thermal stability—could retain the integrity and photothermal effects even after 800 °C thermal annealing, providing inspiring sights into the application under some extreme conditions. After continuous irradiation for twenty times, the photothermal effects of AuNRs coated with R-SiO₂ still remained perfect without performance degradation and shape change. Besides, abundant mesopores could effectively improve the photothermal conversion efficiency of AuNRs. AuNR/R-SiO₂ exhibited an outstanding loading capacity up to 2178 mg g⁻¹ with doxorubicin (DOX) as the model drug, and the release behaviors could be nicely controlled by acidity and near-infrared (NIR) laser to achieve the “On-demand” mode. In vitro experiments showed that AuNR/R-SiO₂ were biocompatible and easy to be internalized by HeLa cells. In addition, due to the ultra-thick silica shell, the effect of the combined chemo-photothermal therapy using AuNR/R-SiO₂/DOX was significantly enhanced, showing a higher therapeutic efficiency than single chem- or photothermal therapy. It was worth noting that AuNR/R-SiO₂ are effective and promising for drug delivery and tumor therapy.

AuNRs coated with ultra-thick SiO₂ shells exhibited ultra-high thermal stability (800 °C), excellent photothermal conversion efficiency (70%) and outstanding loading capacity. The drug release could be nicely controlled by acidity and NIR laser to achieve the “On-demand” mode.     

Synthesis of nanomedicine hydrogel microcapsules by droplet microfluidic process and their pH and temperature dependent release

Chitosan and alginate hydrogels are attractive because they are highly biocompatible and suitable for developing nanomedicine microcapsules. Here we fabricated a polydimethylsiloxane-based droplet microfluidic reactor to synthesize nanomedicine hydrogel microcapsules using Au@CoFeB–Rg3 as a nanomedicine model and a mixture of sodium alginate and PEG-g-chitosan crosslinked by genipin as a hydrogel model. The release kinetics of nanomedicines from the hydrogel were evaluated by simulating the pH and temperature of the digestive tract during drug transport and those of the target pathological cell microenvironment. Their pH and temperature-dependent release kinetics were studied by measuring the mass loss of small pieces of thin films formed by the nanomedicine-encapsulating hydrogels in buffers of pH 1.2, 7.4, and 5.5, which replicate the pH of the stomach, gut and blood, and cancer microenvironment, respectively, at 20 °C and 37 °C, corresponding to the storage temperature of hydrogels before use and normal body temperature. Interestingly, nanomedicine-encapsulating hydrogels can undergo rapid decomposition at pH 5.5 and are relatively stable at pH 7.4 at 37 °C, which are desirable qualities for drug delivery, controlled release, and residue elimination after achieving target effects. These results indicate that the designed nanomedicine hydrogel microcapsule system is suitable for oral administration.

A kind of pH and temperature dependent interpenetrating hydrogel was designed and synthesized via crosslinking of alginate and polyethylene-glycol grafting chitosan by genipin for encapsulated nanomedicine with controlled release.     

Chitosan hydrogel for topical delivery of ebastine loaded solid lipid nanoparticles for alleviation of allergic contact dermatitis

Ebastine, is an antihistamine drug that exerts its effect upon oral administration in humans for the treatment of allergic contact dermatitis (ACD), it also has some systemic side effects like gastric distress, headache, drowsiness, and epistaxis. Moreover, topical corticosteroids are used for treatment of ACD, which causes the human skin to lose its thickness and elasticity. Hence, ebastine-loaded solid lipid nanoparticles (E-SLNs) were prepared and their topical efficacy against allergic contact dermatitis was determined. Compritol 888 ATO and tween 80 were used to prepare E-SLNs by cold dilution of the hot micro-emulsion. E-SLNs were optimized statistically by employing a central composite design using Design-Expert® version 11.0. Optimized E-SLNs showed spherical surface morphology, zeta potential of −15.6 ± 2.4 mV, PDI of 0.256 ± 0.03, and particle sizes of 155.2 ± 1.5 nm and th eentrapment efficiency of ebastine was more than 78%. Nanoparticles were characterized using FT-IR, XRD, and TEM. An E-SLNs loaded hydrogel was prepared using chitosan as a gelling agent and glutaraldehyde as a crosslinker. In vitro drug release studies performed for 24 hours on the E-SLNs dispersion and E-SLNs loaded hydrogel showed a sustained release of maximum 82.9% and 73.7% respectively. In vivo studies were conducted on BALB/c mice to evaluate the topical efficacy of the E-SLNs loaded hydrogel for allergic contact dermatitis. ACD was induced on the ear using picryl chloride solution. After induction, ears were treated daily with the E-SLNs loaded hydrogel for 15 days. Swelling behavior, mast cell count, and histopathological studies of the ear confirmed that the hydrogel alleviated the symptoms of allergic contact dermatitis.

Ebastine exerts its effect upon oral administration in humans for the treatment of allergic contact dermatitis (ACD), but it has some systemic side effects. Hence, ebastine-SLNs loaded hydrogel was prepared to increase topical efficacy of ebastine.     

Self-assembled pH-responsive supramolecular hydrogel for hydrophobic drug delivery

In this study, a novel supramolecular hydrogel system, abbreviated as AGC₁₆/NTS, prepared by molecular self-assembly of cationic gemini surfactant 1,3-bis(N,N-dimethyl-N-cetylammonium)-2-propylacrylatedibromide (AGC₁₆) and anionic aromatic compound trisodium 1,3,6-naphthalenetrisulfonate (NTS), was used to encapsulate hydrophobic model drug curcumin (Cur), constructing a pH-responsive drug delivery system. Cur was effectively encapsulated into the hydrophobic domains of AGC₁₆/NTS through hydrophobic interaction, which was confirmed by ¹H NMR measurement. The effects of Cur on the mechanical strength, phase transition behaviour and morphology of AGC₁₆/NTS were characterized by rheology and cryogenic scanning electron microscopy (cryo-SEM) methods. The pH-responsive release of Cur from AGC₁₆/NTS was obtained and the release amount of Cur ascended with pH value decreasing from 7.4 to 3.0. The hydrodynamic sizes of the released Cur-aggregates determined by dynamic light scattering (DLS) were used to analyse the release process of Cur at different pH. The cell viability assay and cell imaging experiment demonstrated that Cur-loaded hydrogel has much higher cytotoxicity and better cell uptake compared to free Cur. Overall, the AGC₁₆/NTS hydrogel is a prospective material for use in encapsulation and controlled-release of hydrophobic drug molecules.

Supramolecular hydrogel, AGC₁₆/NTS, was used to encapsulate hydrophobic drug curcumin (Cur), constructing a pH-responsive drug delivery system; the uptake of released Cur by cancer cells also occurred.     

Uptake and release of photosensitizers in a hydrogel for applications in photodynamic therapy: the impact of structural parameters on intrapolymer transport dynamics

In this study a hydrogel is presented that can be used as a carrier and release system for photosensitizers. Because of the high structural variety of photosensitizers, four different substances were analysed. Two porphyrins, 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluene-sulfonate) and sodium meso-tetraphenylporphine-4,4′,4′′,4′′′-tetrasulfonat, eosin y and methylene blue were selected. Uptake and release of these photosensitizers were studied. All photosensitizers were taken up by the hydrogel not depending significantly on the structure of the photosensitizer, and it was possible to load the hydrogels in the μmol g⁻¹ range. Nevertheless, size and pK_a value were shown to influence the release behaviour. Finally, the singlet oxygen generation of the photosensitizer after release was demonstrated. The photosensitizer was still highly active and produced a sufficient amount of singlet oxygen.

Different photoactive compounds were evaluated regarding their uptake and release in polymer hydrogels with respect to size, charge, and acidity.