Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro

Manufacturing or using nanomaterials may result in exposure of workers to nanoparticles. Potential routes of exposure include skin, lung and gastrointestinal tract. The lack of health-based standards for nanomaterials combined with their increasing use in many different workplaces and products emphasize the need for a reliable temporary risk assessment tool. Therefore, the aim of this work was to explore the effects of different doses of titanium dioxide nanoparticles on human gastric epithelial cells in vitro. We analyzed proliferation by MTT assay, apoptosis by Tunel, migration by injury assay, oxidative stress by determining GSH/GSSG ratio and DNA damage by Comet assay on nanoparticle-treated AGS human gastric epithelial cell line in comparison to controls. We show and discuss the tumor-like phenotypes of nanoparticles-exposed AGS cells in vitro, as increased proliferation and decreased apoptosis. Our results demonstrate for the first time that nanoparticles induce tumor-like phenotypes in human gastric epithelial cells.     

Theoretical study of D–A′–π–A/D–π–A′–π–A triphenylamine and quinoline derivatives as sensitizers for dye-sensitized solar cells

We have designed four dyes based on D–A′–π–A/D–π–A′–π–A triphenylamine and quinoline derivatives for dye-sensitized solar cells (DSSCs) and studied their optoelectronic properties as well as the effects of the introduction of alkoxy groups and thiophene group on these properties. The geometries, single point energy, charge population, electrostatic potential (ESP) distribution, dipole moments, frontier molecular orbitals (FMOs) and HOMO–LUMO energy gaps of the dyes were discussed to study the electronic properties of dyes based on density functional theory (DFT). And the absorption spectra, light harvesting efficiency (LHE), hole–electron distribution, charge transfer amount from HOMO to LUMO (Q_CT), D index, H_CT index, S_m index and exciton binding energy (E_coul) were discussed to investigate the optical and charge-transfer properties of dyes by time-dependent density functional theory (TD-DFT). The calculated results show that all the dyes follow the energy level matching principle and have broadened absorption bands at visible region. Besides, the introduction of alkoxy groups into triarylamine donors and thiophene groups into conjugated bridges can obviously improve the stability and optoelectronic properties of dyes. It is shown that the dye D4, which has had alkoxy groups as well as thiophene groups introduced and possesses a D–π–A′–π–A configuration, has the optimal optoelectronic properties and can be used as an ideal dye sensitizer.

We have designed four dyes based on D–A′–π–A/D–π–A′–π–A triphenylamine and quinoline derivatives for DSSCs and studied their optoelectronic properties as well as the effects of the introduction of alkoxy groups and thiophene group on the properties.     

Highly selective non-enzymatic electrochemical sensor based on a titanium dioxide nanowire–poly(3-aminophenyl boronic acid)–gold nanoparticle ternary nanocomposite

A novel three component (titanium dioxide nanowire (TiO₂ NW), poly(3-aminophenyl boronic acid) (PAPBA) and gold nanoparticles (Au NPs)) based ternary nanocomposite (TNC) (designated as TiO₂ NW/PAPBA–Au TNC) was prepared by a simple two-stage synthetic approach and utilized for the fabrication of a non-enzymatic (enzyme-free) glucose (NEG) sensor. In stage 2, the PAPBA–Au NC was formed by oxidative polymerization of 3-APBA using HAuCl₄ as oxidant on the surface of pre-synthesized TiO₂ NW via electrospinning (stage 1). The formation of PAPBA–Au NC as the shell on the surface of the TiO₂ NW (core) was confirmed by field emission scanning electron microscopy (FE-SEM). Notably, we obtained a good peak to peak separation, and a high peak current for the redox Fe(CN)₆^3−/4− process indicating excellent electron transfer capability at the glassy carbon electrode (GCE)/TiO₂ NW/PAPBA–Au TNC interface. Also, the fabricated TiO₂ NW/PAPBA–Au TNC provides excellent electrocatalytic activity towards glucose detection in neutral (pH = 7.0) phosphate buffer solution. The detection of glucose was monitored using differential pulse voltammetry. The obtained sensitivity and detection limits are superior to many of the TiO₂ based enzymatic and non-enzymatic glucose sensors reported in the literature. Furthermore, the TiO₂ NW/PAPBA–Au TNC sensor is preferred because of its high selectivity to glucose in the presence of co-existing interfering substances and practical application for monitoring glucose in human blood serum samples.

A highly selective and sensitive enzymeless electrochemical glucose sensor was fabricated based on a novel ternary nanocomposite composed of titanium dioxide nanowire, poly(3-aminophenyl boronic acid) and gold nanoparticles.     

Effect of fluorine substituents on benzothiadiazole-based D–π–A′–π–A photosensitizers for dye-sensitized solar cells

Two D–π–A′–π–A organic dyes with triazatruxene (TAT) as the electron donor, thiophene as the π-spacer, benzoic acid as the anchor group, and benzothiadiazole (BT) or difluorobenzo[c][1,2,5]thiadiazole (DFBT) as the additional acceptor, namely LS101 and LS102, respectively, were applied to dye-sensitized solar cells (DSSCs). As fluorine substituents are usually strong electron-withdrawing groups, introducing two fluorine atoms into BT was expected to strengthen the electron-withdrawing ability of the auxiliary acceptor, resulting in DSSCs with a broader light capture region and further improved power conversion efficiency (PCE). Fluorine is the smallest electron-withdrawing group with an induction effect, but can also act as an electron-donating group owing to its conjugation effect. When the conjugation effect is dominant, the electron-withdrawing ability of additional acceptor DFBT decreases instead. Accordingly, the band gap of LS102 was broadened and the UV-vis absorption spectrum was blue-shifted. In the end, DSSCs based on LS101 achieved a champion PCE of 10.2% (J_sc = 15.1 mA cm⁻², V_oc = 966 mV, FF = 70.1%) while that based on LS102 gave a PCE of only 8.6% (J_sc = 13.4 mA cm⁻², V_oc = 934 mV, FF = 69.1%) under standard AM 1.5G solar irradiation (100 mW cm⁻²) with Co²⁺/Co³⁺ as the electrolyte.

The results and interpretations can clearly explain the reasons for the poor photovoltaic performance of DFBT in DSSCs.     

Lecithin–chitosan–TPGS nanoparticles as nanocarriers of (−)-epicatechin enhanced its anticancer activity in breast cancer cells

Natural compounds such as (−)-epicatechin show a variety of biological properties including anticancer activity. Nonetheless, (−)-epicatechin''s therapeutic application is limited due to its low water solubility and sensitivity to oxygen and light. Additionally, previous studies have reported that the encapsulation of flavonoids in nanoparticles might generate stable deliverable forms, which improves the availability and solubility of the bioactive compounds. The aims of this study were to generate (−)-epicatechin-loaded lecithin–chitosan nanoparticles (EC-LCT-NPs) by molecular self-assembly and to assess their cytotoxic potential against breast cancer cells. Various parameters were measured to characterize the EC-LCT-NPs including size, polydispersity index (PdI), zeta potential, morphology and entrapment efficiency. The results showed that the mean particle size of the EC-CLT-NPs was 159 ± 2.23 nm (PdI, 0.189), and the loading and entrapment efficiencies of (−)-epicatechin were 3.42 ± 0.85% and 56.1 ± 3.9%, respectively. The cytotoxic effect of the EC-CLT-NPs was greater than that of free (−)-epicatechin on breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-436 and SK-Br3). Indeed, EC-LCT-NPs showed an IC₅₀ that was four-fold lower (85 μM) than free (−)-epicatechin (350 μM) and showed selectivity to cancerous cells. This study demonstrated that encapsulating (−)-epicatechin into lecithin–chitosan nanoparticles opens new options for breast cancer treatment.

Natural compounds such as (−)-epicatechin show a variety of biological properties including anticancer activity.     

X-ray powder diffraction to analyse bimetallic core–shell nanoparticles (gold and palladium; 7–8 nm)

A comparative X-ray powder diffraction study on poly(N-vinyl pyrrolidone) (PVP)-stabilized palladium and gold nanoparticles and bimetallic Pd–Au nanoparticles (both types of core–shell nanostructures) was performed. The average diameter of Au and Pd nanoparticles was 5 to 6 nm. The two types of core–shell particles had a core diameter of 5 to 6 nm and an overall diameter of 7 to 8 nm, i.e. a shell thickness of 1 to 2 nm. X-ray powder diffraction on a laboratory instrument was able to distinguish between a physical mixture of gold and palladium nanoparticles and bimetallic core–shell nanoparticles. It was also possible to separate the core from the shell in both kinds of bimetallic core–shell nanoparticles due to the different domain size and because it was known which metal was in the core and which was in the shell. The spherical particles were synthesized by reduction with glucose in aqueous media. After purification by multiple centrifugation steps, the particles were characterized with respect to their structural, colloid-chemical, and spectroscopic properties, i.e. particle size, morphology, and internal elemental distribution. Dynamic light scattering (DLS), differential centrifugal sedimentation (DCS), atomic absorption spectroscopy (AAS), ultraviolet-visible spectroscopy (UV-vis), high-angle annular dark field imaging (HAADF), and energy-dispersed X-ray spectroscopy (EDX) were applied for particle characterization.

A comparative X-ray powder diffraction study on poly(N-vinyl pyrrolidone) (PVP)-stabilized palladium and gold nanoparticles and bimetallic Pd–Au nanoparticles (both types of core–shell nanostructures) was performed.     

Regio-regular alternating diketopyrrolopyrrole-based D1–A–D2–A terpolymers for the enhanced performance of polymer solar cells

We designed and synthesized regio-regular alternating diketopyrrolopyrrole (DPP)-based D₁–A–D₂–A terpolymers (PDPPF2T2DPP-T2, PDPPF2T2DPP-TVT, and PDPPF2T2DPP-DTT) using a primary donor (D₁) [3,3′-difluoro-2,2′-bithiophene (F2T2)] and a secondary donor (D₂) [2,2′-bithiophene (T2), (E)-1,2-di(thiophen-2-yl)ethene (TVT), or dithieno[3,2-b:2′,3′-d]thiophene (DTT)]. A PDPP2DT-F2T2 D–A polymer was synthesized as well to compare optical, electronic, and photovoltaic properties. The absorption peaks of the terpolymers (PDPPF2T2DPP-T2, PDPPF2T2DPP-TVT, and PDPPF2T2DPP-DTT) were longer (λ_max = 801–810 nm) than the peak of the PDPP2DT-F2T2 polymer (λ_max = 799 nm), which is associated with the high-lying HOMO levels of the terpolymers (−5.08 to −5.13 eV) compared with the level of the PDPP2DT-F2T2 polymer (−5.38 eV). The photovoltaic properties of these DPP-based polymers were investigated under simulated AM 1.5G sunlight (100 mW cm⁻²) with a conventional structure (ITO/PEDOT:PSS/polymer:PC₇₁BM/Al). The open-circuit voltages (V_oc) of photovoltaic devices containing the terpolymers were slightly lower (0.68–0.70 V) than the V_oc of the device containing the PDPP2DT-F2T2 polymer (0.79 V). The short-circuit current (J_sc) of the PDPPF2T2DPP-DTT device was significantly improved (14.14 mA cm⁻²) compared with that of the PDPP2DT-F2T2 device (8.29 mA cm⁻²). As a result, the power conversion efficiency (PCE) of the PDPPF2T2DPP-DTT device (6.35%) was increased by 33% compared with that of the simple D–A-type PDPP2DT-F2T2 device (4.78%). The highest J_sc and PCE values (the PDPPF2T2DPP-DTT device) were attributed to an optimal nanoscopically mixed morphology and strong interchain packing with a high face-on orientation in the blend film state. The study demonstrated that our strategy of using multiple donors in a regio-regular alternating fashion could fine-tune the optical, electronic, and morphological properties of D–A-type polymers, enhancing the performance of polymer solar cells.

We designed and synthesized regio-regular alternating diketopyrrolopyrrole (DPP)-based D₁–A–D₂–A terpolymers (PDPPF2T2DPP-T2, PDPPF2T2DPP-TVT, and PDPPF2T2DPP-DTT) for use in polymer solar cells.     

Synthesis and biological characterization of alloyed silver–platinum nanoparticles: from compact core–shell nanoparticles to hollow nanoalloys

Bimetallic nanoparticles consisting of silver and platinum were prepared by a modified seeded-growth process in water in the full composition range in steps of 10 mol%. The particles had diameters between 15–25 nm as determined by disc centrifugal sedimentation (DCS) and transmission electron microscopy (TEM). Whereas particles with high platinum content were mostly spherical with a solid silver core/platinum shell structure, mostly hollow alloyed nanoparticles were observed with increasing silver content. The internal structure and the elemental distribution within the particles were elucidated by high-resolution transmission electron microscopy (HRTEM) in combination with energy-dispersive X-ray spectroscopy (EDX). The particles were cytotoxic for human mesenchymal stem cells (hMSC) above 50 mol% silver. This was explained by dissolution experiments where silver was only released at and above 50 mol% silver. In contrast, platinum-rich particles (less than 50 mol% silver) did not release any silver ions. This indicates that the presence of platinum inhibits the oxidative dissolution of silver.

Bimetallic nanoparticles consisting of silver and platinum were prepared by a modified seeded-growth process in water in the full composition range in steps of 10 mol%.     

Monoclonal antibody conjugated magnetic nanoparticles could target MUC‐1‐positive cells in vitro but not in vivo

MUC1 antigen is recognized as a high‐molecular‐weight glycoprotein that is unexpectedly over‐expressed in human breast and other carcinomas. In contrast, C595 a monoclonal antibody (mAb) against the protein core of the human urinary epithelial machine, is commonly expressed in breast carcinomas. The aim of this study was to conjugate ultra‐small super paramagnetic iron oxide nanoparticles (USPIO) with C595 mAb, in order to detect in vivo MUC1 expression. A dual contrast agent (the C595 antibody‐conjugated USPIO labeled with 99mTc) was prepared for targeted imaging and therapy of anti‐MUC1‐expressing cancers. The C595 antibody‐conjugated USPIO had good stability and reactivity in the presence of blood plasma at 37 °C. No significant differences were observed in immunoreactivity results between conjugated and nonconjugated nanoparticles. The T₁ and T₂ measurements show >79 and 29% increments (for 0.02 mg/ml iron concentrations) in T₁ and T₂ values for USPIO‐C595 in comparison with USPIO, respectively. The nanoprobes showed the interesting targeting capability of finding the MUC1‐positive cell line in vitro. However, we found disappointing in vivo results (i.e. very low accumulation of nanoprobes in the targeted site while >80% of the injected dose per gram was taken up by the liver and spleen), not only due to the coverage of targeting site by protein corona but also because of absorption of opsonin‐based proteins at the surface of nanoprobes. Copyright © 2014 John Wiley & Sons, Ltd.     

Characterization and simulation study of organic solar cells based on donor–acceptor (D–π–A) molecular materials

In this study, the analysis of microelectronic and photonic structure in a one dimension program [AMPS-1D] has been successfully used to study organic solar cells. The program was used to optimize the performance of organic solar cells based on (carbazole-methylthiophene), benzothiadiazole and thiophene [(Cbz-Mth)-B-T]2 as electron donors, and [6,6]-phenyl-C₆₁-butyric acid methyl ester (PCBM) as an electron acceptor. The optoelectronic properties of these dyes were investigated by using the Density Functional Theory DFT/B3LYP/6-31G(d,p) method. We studied the influence of the variation of the thickness of the active layer, the temperature, and the density of the effective states of the electrons and the holes in the conduction and valence bands respectively on the performance of the solar cells based on [(Cbz-Mth)-BT]2–PCBM as a photoactive material, sandwiched between a transparent indium tin oxide (ITO) and an aluminum (Al) electrode. The addition of other thiophene units in the copolymer or the deposition of a layer of PEDOT between the anode (ITO) and the active layer, improves the performances of the cell, especially resulting in a remarkable increase in the value of the power conversion efficiency (PCE).

The solar cell ITO/PEDOT/[(Cbz-Mth)-B-DT]2-A:PCBM/Al under study and the results obtained, including a power conversion efficiency of 11%. The impact of several parameters on the performance has been studied to obtain the optimal device architecture.     

Scanning atmospheric-pressure plasma jet treatment of nickel oxide with peak temperature of ∼500 °C for fabricating p–i–n structure perovskite solar cells

Scanning atmospheric-pressure plasma jet (APPJ) treatment of nickel oxide with a peak temperature of 500 °C was performed for fabricating p–i–n structure perovskite solar cells (PSCs). APPJ post-treatment increases the haze of NiO on FTO glass, leading to enhanced light scattering in PSCs that in turn improves the cell efficiency. APPJ treatment on NiO also improves the wettability to facilitate the follow-up deposition of CH₃NH₃PbI₃. This also leads to better PSC performance. X-ray photoelectron spectroscopy indicates that APPJ treatment results in fewer C–N bonds and reduced NiAc₂ content, suggesting more complete conversion of the liquid precursor into NiO. With three APPJ scans, the average PCE improves from 11.91% to 13.47%, with the best-performing PSC achieving an efficiency of 15.67%.

Scanning atmospheric-pressure plasma jet (APPJ) treatment of nickel oxide with a peak temperature of 500 °C was performed for improving the performance of p–i–n structure perovskite solar cells (PSCs).     

Antioxidant and anti-aging potential of a peptide formulation (Gal2–Pep) conjugated with gallic acid

Skin is highly vulnerable to premature aging due to external stress, therefore, in this study, a peptide formulation, (galloyl)₂–KTPPTTP (Gal₂–Pep) was synthesized by combining TPPTTP peptide, and gallic acid (GA). All peptides were synthesized on 2-chlorotrityl chloride resin using solid-phase peptide synthesis (SPPS), and analyzed on an electrospray ionization (ESI)/quadrupole-time-of-flight (Q-TOF) tandem mass spectroscopy (MS) system. Initially, Gal₂–Pep showed no toxicity below the concentration 100 μM with cell survival rate of 88% for keratinocytes and fibroblasts. The reactive oxygen species (ROS) scavenging activity of Gal₂–Pep was more stable compared to GA alone; and after four weeks at room temperature, its ROS scavenging activity remained higher than 50%. Moreover, the peptide formulation, Gal₂–Pep also exhibited elastase inhibitory effect in CCD-1064Sk fibroblast cells. Based on the results of RT-qPCR, it was proved in this study that Gal₂–Pep increased the expression of PGC-1α to prevent oxidative stress, and validated its potential as an anti-aging agent through increasing the expression of type I collagen and by decreasing the expression of matrix metalloproteinase-1 (MMP1). The findings obtained reinforce the suggestion that the peptide formulation synthesized in this study could be used as a natural antioxidant and anti-aging agent for its cosmetic applications.

Skin is highly vulnerable to premature aging due to external stress, therefore, in this study, a peptide formulation, (galloyl)₂–KTPPTTP (Gal₂–Pep) was synthesized by combining TPPTTP peptide, and gallic acid (GA).     

Activation of ZrO2–WO3 solid acid catalysts in a Friedel–Crafts reaction through post-hydrothermal treatment

ZrO₂–WO₃ mixed oxide plays an essential role in the chemical and petroleum industries. So far, very little work has paid attention to the activation of the low activity of ZrO₂–WO₃ catalysts. In this work, poorly reactive ZrO₂–WO₃ was prepared as a model catalyst by a sol–gel method and it was accompanied by post-hydrothermal treatment with various solutions. The catalytic results in the Friedel–Crafts reaction of anisole and benzyl alcohol showed that the post-hydrothermal treatment with ethylenediamine or ammonium hydroxide solutions dramatically improved the activity of ZrO₂–WO₃, while the hydrothermal treatments with water or ammonia chloride solution resulted in poorer activity and selectivity. The former treatments were found to induce a huge transformation of the ZrO₂ crystal from monoclinic to tetragonal as well as a significant increase in acidic WO_x clusters that anchored onto ZrO₂. The generation of the WO_x clusters was responsible for the activation of ZrO₂–WO₃.

The high pH value of the post-hydrothermal treatment induces the generation of acidic WO_x cluster active sites.     

Carbon nanotube–titanium dioxide nanocomposite support for improved activity and stability of an iridium catalyst toward the oxygen evolution reaction

In order to improve the electrocatalytic activity and stability of an iridium (Ir) nanoparticle catalyst toward the oxygen evolution reaction (OER) in acidic electrolyte, carbon nanotube and titanium dioxide nanocomposites (CNT@TiO₂) are presented as a high-performance support. TiO₂ was synthesized on CNTs by using a novel layer-by-layer solution coating method that mimics atomic layer deposition (ALD) but is cost-effective and scalable. In the nanocomposites, CNTs serve as the electron pathways and the surface TiO₂ layers protect CNTs from corrosion under the harsh OER conditions. Thus, CNT@TiO₂ demonstrates excellent corrosion resistance as well as a high electrical conductivity (1.6 ± 0.2 S cm⁻¹) comparable to that of Vulcan carbon (1.4 S cm⁻¹). The interaction between Ir and TiO₂ promotes the formation of Ir(iii) species, thereby enhancing the OER activity and stability of the Ir nanoparticle catalyst. Compared to commercial carbon-supported Ir (Ir/C) and Ir black catalysts, CNT@TiO₂-supported Ir exhibits superior OER activity and stability.

To improve the electrocatalytic activity and stability of an iridium nanoparticle catalyst toward the oxygen evolution reaction, carbon nanotube and titanium dioxide nanocomposites (CNT@TiO₂) are presented as a high-performance support.     

A novel study on preparation of H2TiO3–lithium adsorbent with titanyl sulfate as titanium source by inorganic precipitation–peptization method

A peroxy lithium titanate sol was prepared with low-cost and easily available titanyl sulfate as the titanium source, lithium acetate as the lithium source, and aquae hydrogenii dioxidi as the complexing agent using an inorganic precipitation–peptization method. The sol system was aged, centrifugal-washed, dried and calcined to obtain a pure precursor, Li₂TiO₃, followed by pickling with hydrochloric acid to obtain the H₂TiO₃–lithium adsorbent. The effects of aging time and calcination temperature on the target product were investigated. The results indicate that the sol-system is stable, which is beneficial for loading on a suitable carrier, such as ceramic foams. Centrifugal-washing, instead of vacuum filtration-washing, is conducive to product formation. The most suitable aging time of precursor sol is 24 h and the appropriate calcination temperature is 750 °C. The lithium drawn-out ratio of samples synthesized in this condition reaches 89.50% after pickling with 0.2 M hydrochloric acid for 8 h at 70 °C. Moreover, the Li⁺ uptake of the adsorbent (adsorption capacity) reaches 29.96 mg g⁻¹ and 33.35 mg g⁻¹ when the adsorption time is 1 h and 8 h, respectively.

A peroxy Li₂TiO₃ sol was prepared with low-cost TiOSO₄ as titanium source, CH₃COOLi as lithium source, and H₂O₂ as complexing agent by inorganic precipitation–peptization method.     

Large-area perovskite solar cells – a review of recent progress and issues

In recent years, perovskite solar cells (PSCs) have attracted great attention in the photovoltaic research field, because of their high-efficiency (certified 22.1%) and low-cost. In this review paper, we briefly introduce the history of efficiency development for PSCs, and discuss some of the major problems for large-area (≥1 cm²) PSC devices. In addition, we summarize the recent progress in the aspects of fabrication methods for large-area perovskite films, and improving the efficiency and stability of the large-area PSC devices. Finally, we give a short summary and outlook of large-area PSC devices. This article is mainly organized into three parts. The first part focuses on the main fabricating technologies for large-area perovskite films. The second section discusses some methods that are used to improve the efficiency of PSCs. In the last part, different approaches are used to improve the stability of PSCs.

In this review, we summarize the recent progress in the aspects of the fabrication methods for large-area perovskite films, improving the efficiency and stability of the large-area PSC devices.     

Cul4A-DDB1–mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine

Although serine metabolism plays a crucial role in the proliferation and survival of tumor cells, how it supports tumor cell migration remains poorly understood. Phosphoglycerate dehydrogenase (PHGDH) catalyzes the oxidation of 3-phosphoglycerate to 3-phosphonooxypyruvate, the first committed step in de novo serine biosynthesis. Here we show that PHGDH was monoubiquitinated by cullin 4A–based E3 ligase complex at lysine 146 in colorectal cancer (CRC) cells, which enhanced PHGDH activity by recruiting a chaperone protein, DnaJ homolog subfamily A member 1, to promote its tetrameric formation, thereby increasing the levels of serine, glycine, and S-adenosylmethionine (SAM). Increased levels of SAM upregulated the expression of cell adhesion genes (laminin subunit gamma 2 and cysteine rich angiogenic inducer 61) by initiating SET domain containing 1A–mediated trimethylation of histone H3K4, thereby promoting tumor cell migration and CRC metastasis. Intriguingly, SAM levels in tumors or blood samples correlated with the metastatic recurrence of patients with CRC. Our finding not only reveals a potentially new role and mechanism of SAM-promoted tumor metastasis but also demonstrates a regulatory mechanism of PHGDH activity by monoubiquitination.     

The pH-triggered drug release and simultaneous carrier decomposition of effervescent SiO2–drug–Na2CO3 composite nanoparticles: to improve the antitumor activity of hydrophobic drugs

To achieve a better release effect of hydrophobic drugs and spontaneous nanocarrier disintegration by dissolution as well as the CO₂ production of Na₂CO₃ further, improving the therapeutic effect of hydrophobic drugs, and thereby avoiding the accumulation of the nanocarrier in vivo to produce organ toxicity, effervescent SiO₂–drug–Na₂CO₃ composite nanoparticles (ESNs) were prepared in this study using a tetraethyl orthosilicate hydrolysis method. Sodium carbonate was used as the effervescent disintegrant to respond to the acidic microenvironment of the tumor. The properties of ESNs were assessed and TEM images were taken to verify the self-disintegration characteristics of nanocarrier materials. The in vitro anticancer efficacy of ESNs was evaluated in human breast cancer MCF-7 cells. ESNs loaded with hydrophobic drugs were successfully constructed, and showed high entrapment efficiency and drug loading. The nanocarrier successfully achieved self-disintegration in a PBS environment of pH value at 5.0, and showed excellent antitumor effect in vitro. ESNs can effectively load hydrophobic drugs and achieve self-disintegration, while avoiding toxicity from the accumulation of the nanocarrier. These results suggest that ESNs are a promising drug delivery system capable of maximizing the anticancer therapeutic efficacy and minimizing the systemic toxicity.

Effervescent SiO₂–drug–Na₂CO₃ composite nanoparticles were prepared in this study using a tetraethyl orthosilicate hydrolysis method to achieve a better release effect of hydrophobic drugs and spontaneous nanocarrier disintegration by dissolution.     

In vivo switch to IL-10–secreting T regulatory cells in high dose allergen exposure

High dose bee venom exposure in beekeepers by natural bee stings represents a model to understand mechanisms of T cell tolerance to allergens in healthy individuals. Continuous exposure of nonallergic beekeepers to high doses of bee venom antigens induces diminished T cell–related cutaneous late-phase swelling to bee stings in parallel with suppressed allergen-specific T cell proliferation and T helper type 1 (Th1) and Th2 cytokine secretion. After multiple bee stings, venom antigen–specific Th1 and Th2 cells show a switch toward interleukin (IL) 10–secreting type 1 T regulatory (Tr1) cells. T cell regulation continues as long as antigen exposure persists and returns to initial levels within 2 to 3 mo after bee stings. Histamine receptor 2 up-regulated on specific Th2 cells displays a dual effect by directly suppressing allergen-stimulated T cells and increasing IL-10 production. In addition, cytotoxic T lymphocyte–associated antigen 4 and programmed death 1 play roles in allergen-specific T cell suppression. In contrast to its role in mucosal allergen tolerance, transforming growth factor β does not seem to be an essential player in skin-related allergen tolerance. Thus, rapid switch and expansion of IL-10–producing Tr1 cells and the use of multiple suppressive factors represent essential mechanisms in immune tolerance to a high dose of allergens in nonallergic individuals.     

Functional drug carriers formed by RGD-modified β-CD-HPG for the delivery of docetaxel for targeted inhibition of nasopharyngeal carcinoma cells

In this study, a drug delivery system was prepared by grafting the targeting molecule arginine-glycine-aspartic acid (RGD) onto hyperbranched polyglycerol (HPG)-modified β-cyclodextrin (β-CD-HPG) for the targeted inhibition of nasopharyngeal carcinoma (NPC) cells. The obtained β-CD-HPG-RGD with a relatively small size and low surface charge delivered docetaxel (Doc) effectively and displayed a targeting effect to human NPC HNE-1 cells, as confirmed by confocal laser scanning microscopy and flow cytometry. The in vitro drug release analysis exhibited the controlled drug release kinetics of the β-CD-HPG-RGD/Doc nanomedicine. β-CD-HPG-RGD/Doc effectively inhibited the proliferation of HNE-1 cells and promoted apoptosis. Moreover, its biocompatibility in vitro and in vivo was assessed. The results indicate that the β-CD-HPG-RGD/Doc nanomedicine has potential application in NPC targeting therapy.

A new RGD targeting drug carrier based on HPG-modified β-CD has been synthesized. This carrier showed excellent biocompatibility and targeting ability. The obtained NPs could effectively inhibit the proliferation of tumor cells.