骨髓增生异常综合征患者血清白细胞介素8的检测

目的：探讨骨髓增生异常综合征（ＭＤＳ）患者血清白细胞介素８（ＩＬ－８）检测的临床价值。方法：夹心酶联免疫吸附法（ＥＬＩＳＡ法）检测１６例ＭＤＳ患者血清ＩＬ－８,并与正常对照组比较及ＭＤＳ亚型间比较。结果：ＭＤＳ患者血清ＩＬ－８水平显著高于正常对照组（Ｐ＜０ ．０５）,ＲＡＥＢ／ＲＡＥＢ－ｔ组显著高于ＲＡ／ＲＡＳ组（Ｐ＜０．０５）。结论：ＩＬ－８在ＭＤＳ的发病中有一定作用,ＩＬ－８的检测可为ＭＤＳ的疗效判断及预后转归提供有价值的线索。     

雷公藤单体TZ93对人外周血单个核细胞表型的影响

研究雷公藤治疗系统性红斑狼疮的作用机制，以激活的正常外周血单个核细胞（ＰＢＭＣ）为细胞模型，应用 荧光激活细胞分类 仪（ＦＡＣＳ）分析以藤单体ＴＺ９３对ＰＢＭＣ细胞表型的影响。１０名正常女性的ＰＢＭＣ分成３组：Ｉ组ＰＢＭＣ，Ⅱ组ＰＢＭＣ＋ＰＨＡ，Ⅲ且ＰＢＭ＋ＰＨＡ＋ＴＺ９３，分别加入鼠抗入ＣＤ８、ＣＤ４、ＣＤ２５、ＮＫ抗体。结果：ＦＡＣＳ显示ＴＺ９３能显著抑制激活的ＰＢＭＣ中ＣＤ４、ＣＤ８、ＣＤ２     

体外循环心内直视手术中细胞因子的变化及其意义

目的：了解体外循环（ＣＰＢ）心内直视手术中细胞因子的变化，并探讨其临床意义。方法：测定心内直视手术病人不同时间的ＩＬ－６、ＩＬ－８和ＭＤＡ，应用ＥＬＩＳＡ法测定ＩＬ－６，ＩＬ－８，ＴＢＡ比色法测定ＭＤＡ。结果：ＩＬ－６，ＩＬ－８和ＭＤＡ在体外循环结束后明显高于体外循环前（Ｐ〈０．０５）。ＩＬ－８与ＣＰＢ时间及ＭＤＡ呈正相关。结论：作为免疫细胞激活剂，ＩＬ－６，ＩＬ－８参与了炎性反应及缺血和再灌注损     

体外循环心内直视手术中细胞因子的变化及其意义

目的：了解体外循环（ＣＰＢ）心内直视手术中细胞因子的变化,并探讨其临床意义。方法：测定心内直视手术病人不同时间的ＩＬ－６、ＩＬ－８和ＭＤＡ,应用ＥＬＩＳＡ法测定ＩＬ－６、ＩＬ－８,ＴＢＡ比色法测定ＭＤＡ。结果：ＩＬ－６、ＩＬ－８和ＭＤＡ在体外循环结束后明显高于体外循环前（Ｐ＜００５）。ＩＬ－８与ＣＰＢ时间及ＭＤＡ呈正相关。结论：作为免疫细胞激活剂,ＩＬ－６、ＩＬ－８参与了炎性反应及缺血和再灌注损伤的过程     

严重烧伤后营养支持途径对机体肠道功能的影响

目的：考察严重烧伤早期两种营养支持途径对机体肠道功能的影响。方法：１９例烧伤病人随机分为早期肠道营养组（ＥＮ）和肠外营养组（ＰＮ）,分别于伤后１、４、８、１４ｄ测胃泌素、胃动素、ＭＤＡ、ＳＯＤ、内毒素、ＴＮＦ、ＤＡＯ及肠道通透性。结果：ＥＮ组血胃泌素、胃动素ＰＢＤ４、ＰＢＤ８显著高于ＰＮ组（Ｐ〈０．０５,Ｐ〈０．０１）。血浆ＭＤＡ在ＰＢＤ４、ＰＢＤ８显著低于ＰＮ组（Ｐ〈０．０１）,ＥＮ组ＳＯＤ在Ｐ     

狼疮性肾炎血清酶的变化及临床意义

目的：研究狼疮性肾炎（ＬＮ）患者血清酶的变化及临床意义。方法：通过全自动生化分析仪检验３４例ＬＮ患者及３０名健康体检者血中８种血清酶。结果：ＬＮ组较健康组ＬＤＨ、ＨＢＤＨ、ＡＳＴ、ＧＧＴ（ｐ〈０．０１）、ＡＬＰ（ｐ〈０．０５）明显升高,均有显著性意义,而ＣＫ、ＡＭＹ和ＡＬＴ（ｐ〉０．０５）无显著性意义。ＬＤＨ和ＨＢＤＨ（ｐ〈０．０００５）之间有高度相关性。结论：ＬＤＨ、ＨＢＤＨ、ＡＳＴ、ＧＧＴ和Ａ     

地塞米松及雷公藤甲素对哮喘CD4、CD8细胞凋亡的影响

目的：探讨地塞米松（ＤＭ）和雷公藤对哮喘ＣＤ４、ＣＤ８细胞凋亡的作用。方法：采用卵蛋白致敏复制哮喘豚鼠模型。哮喘豚鼠经腹腔内注射ＤＭ和雷公藤甲素（ＴＰ）后,用淋巴细胞分离液的密度梯度离心分离外周血单个核细胞（ＰＢＭＣｓ）。采用免疫细胞化学（ＩＣＣ）与３’末端转移酶介导的脱碱磷酸尿苷（ｄＵＴＰ）缺口末端标记（ＴＵＮＥＬ）双标染色对ＣＤ４、ＣＤ８细胞凋亡进行检测。结果：ＤＭ和ＴＰ均能诱导哮喘外周血ＣＤ     

介入放射环境影响人T-Sub、CD25、CD16的观察与防护

目的：现察介入放射诊疗人员防护前后的 Ｔ细胞亚群、ＩＬ－２Ｒ（ＣＤ２５）、ＮＫ细胞（ＣＤ１６）的影响．方法：左３２名放射科人员中选择从事介入放射诊疗１０年以上，慢性疲劳症状突出者１０人为现察对象；使用功能性食品抗疲Ｉ号胶囊防护，设防护前组（ＢＧ）、防护后组 （ＡＧ）、对照组（ＣＧ），使用流式细胞仪测定防护前后的 Ｔ细胞亚群、ＣＤ２５、ＣＤ１６。结果：ＡＧ与ＢＧ相比，总Ｔ细胞ＣＤ３上升，亚群中的ＣＤ８呈上升，ＣＤ４略有下降，ＣＤ４／ＣＤ８下降，ＣＤ２５和ＣＤ１６上升（Ｐ＜０.０５，Ｐ＜０．０１）；ＢＧ与ＣＧ相比，ＣＤ３、ＣＤ４、ＣＤ８、ＣＤ１６、ＣＤ２５均呈低下水平（Ｐ＜０.０５，Ｐ＜０.０１），ＣＤ４／ＣＤ８。比值出现异常升高等．结论：长期从事医疗辐射环境在危害机体造血的同时，也危害Ｔ细胞亚群及 Ｔ细胞 ＣＤ１６、ＣＤ２５的表达，Ｉ胶囊有明显的改善造血、调节细胞免疫功能的抗辐射作用．     

反流性肾病患儿T淋巴细胞免疫活性的变化及其意义

目的：探讨细胞免疫在反返流性肾病（ＲＮ）发病中的作用。方法：通过流式细胞（ＦＣＭ），应用单标和双标免疫荧光法，利用单顾隆抗体测定１２例ＲＮ患儿外周血单个核细胞（ＰＢＭＣ）表面膜抗原，从而分析ＲＮ患者Ｔ淋巴亚群及茯活化情况。结果：Ｔ淋巴细胞亚群ＣＤ３、ＣＤ４与正常组相仿，ＣＤ８增高，ＣＤ４／ＣＤ８比值低于正常；Ｔ细胞ＣＤ２５高于正常，Ｔ细胞亚群活化表达ＣＤ３ＣＤ２４５、ＣＤ４ＣＤ２５、ＣＤ８ＣＤ２５     

脱钙骨基质治疗良性骨肿瘤及瘤样病变骨缺损

目的：介绍动用胎儿脱钙骨基质（ＤＢＭ）治疗良性骨肿瘤及瘤样病变骨缺损的方法及疗效。方法：对７２例良性骨肿瘤及瘤样病变患者病灶清除术后遗留的骨缺损,以ＤＢＭ进行充填。结果：ＤＢＭ植入后４周,骨缺损处已有新骨生成,８周以后成骨作用显著,密度逐渐均匀,相互连成一片。１０～１２周密度接近正常骨组织。ＤＢＭ有促进成骨作用。结论：ＤＢＭ具有良好的组织相容性和诱导成骨作用。彻底的病灶清除,合适量的ＤＢＭ植入残腔     

男性腰椎和股骨颈骨密度测量的临床价值

目的:探讨测量男性腰椎、股骨颈骨密度的临床价值.方法:对224例20～74岁成年男性的腰椎和股骨颈行定量CT骨密度测量,并对结果进行分析.结果:成年男性腰椎和股骨颈骨密度峰值年龄为20～24岁,35岁以后股骨颈骨密度下降速率大于腰椎,二者累积丢失率差异具有显著性(P<0.05).结论:男性股骨颈的骨密度的测量值较腰椎更具有临床意义.     

强直性脊柱炎患者骨密度的影响因素分析

目的:分析强直性脊柱炎（AS）患者腰椎和股骨颈骨密度（BMD）改变与年龄、性别、病程、髋关节病变及急性时相反应物之间的关联。方法:选取91例确诊AS患者,采用双能X线吸收法测定腰椎（L₂~L₄）、股骨颈BMD值,骨质疏松诊断标准用T值表示。结果:男性AS患者腰椎、股骨颈BMD均低于女性AS患者（P<0.05）。AS合并髋关节病变患者与无髋关节病变患者股骨颈BMD差异无统计学意义（P>0.05）。患者年龄与腰椎BMD T值呈负相关关系（P<0.05）,而患者病程、急性时相反应物与AS患者BMD均无相关关系（P>0.05）。结论:AS患者男性比女性更易发生骨量减少和骨质疏松,随着年龄的增加,腰椎骨质疏松发生增多。病程、髋关节病变以及急性时相反应物对AS患者BMD改变无显著影响。     

老年男性骨矿物含量变化的临床研究

为探讨男性骨质疏松的影响及危害,用双能X线骨密度测量仪（DEXA）对284例老年男性腰椎及股骨骨矿物含量进行测定,得出随着年龄的增长,骨矿物含量逐渐下降,其骨量丢失的变化规律为：股骨Wards三角＞股骨颈＞腰椎（L2～L4）＞股骨粗隆。骨质疏松发病率在70y以后明显增加,骨质疏松骨折发生率为18％。提示：男性骨质疏松发生率之高及其造成的危害应引起人们高度重视,骨密度（BMD）测量尤其是髋部骨量测定,对骨质疏松早期诊断及其骨折的预防有着重要的意义。     

母患骨质疏松的女儿呈低峰值骨量吗?

目的研究骨质疏松母亲的女儿峰值骨量是否比正常母亲的女儿更低,并初步探讨导致这一差异的因素。方法从上海市各社区招募401个汉族核心家庭(包括父母双亲和一名20～40岁的健康女儿),用双能X线吸收仪测定腰椎(L1-4)和股骨近端各部位骨密度(BMD),剔除父亲患骨质疏松或骨量减少的家庭,获得126个骨质疏松母亲及其女儿和136个年龄匹配的BMD正常母亲及其女儿。结果骨质疏松母亲的女儿L1-4、股骨颈和大转子各部位BMD值(0.94g/cm2±0.10g/cm2,0.75g/cm2±0.10g/cm2,0.61g/cm2±0.08g/cm2,显著低于正常母亲的女儿(1.00g/cm2±0.11g/cm2,0.80g/cm2±0.11g/cm2,0.66g/cm2±0.09g/cm2,均P<0.001),且体重也显著低于正常母亲的女儿(P<0.05),两组间年龄、身高、月经初潮年龄、牛奶摄入者比例、摄入量等差异均无统计学意义。多因素逐步回归分析显示:体重是影响女儿各部位BMD的最重要因素,分别解释L1-4、股骨颈和大转子BMD值变异的9.4%、16.5%和16.6%。剔除体重因素后,母亲患骨质疏松成为影响女儿BMD的最重要因素,分别解释L1-4、股骨颈和大转子的BMD值变异的5.1%、5.3%和4.2%。结论母患骨质疏松的女儿具有低的峰值骨量,体重是最重要的影响因素,其次为母亲具低的BMD。     

细胞色素P450c 17α基因启动子区Msp AI多态性与上海市汉族男性骨量、骨大小的关系

目的探讨细胞色素P450c 17α(CYP17)基因启动子区Msp AI多态性与上海市汉族男性骨量、骨大小的关系.方法应用聚合酶链反应-限制性片段长度多态性检测上海市397例46～80岁无亲缘关系的汉族男性(其中324例健康男性,73例骨质疏松患者)CYP17基因Msp AI多态性,并用双能X线吸收仪检查研究对象腰椎1～4和股骨近端股骨颈、大转子区及Ward's三角部位骨密度(BMD)、骨矿含量(BMC)和骨截面大小(CSA).结果397例男性CYP17 Msp AI基因型频率分布依次为TC(51.1%)、CC(33.8%)和TT(15.1%),等位基因T和C频率分布分别为40.7%和59.3%,等位基因频率分布符合Hardy-Weinberg定律.未发现该多态性在73例骨质疏松患者和324例健康男性之间频率分布的差异.通过协方差分析,在397例受试者、73例骨质疏松患者或324例健康男性中,均未显示CYP17基因Msp AI多态性与腰椎1～4和股骨近端各部位BMD、BMC和CSA的相关性.结论CYP17基因MspAI多态性不是影响上海市汉族男性骨量和骨大小变异的遗传因素.     

佛山地区女性峰值骨量影响因素研究

目的研究佛山地区健康女性峰值骨量的影响因素,为下一步骨质疏松症的预防和治疗提供科学依据。方法对本地区1317名健康体检女性行腰椎骨密度测定,以问卷形式进行可能影响骨量的有关因素调查,分析峰值骨量的影响因素。结果峰值骨量与体重指数、运动、营养呈显著正相关,而月经初潮、母系骨折史与峰值骨量呈负相关。结论月经初潮、母系骨折史、体重、运动、牛奶摄入等因素与本地区女性人群峰值骨量有密切关系。     

Bone density of élite female athletes with stress fractures

To investigate whether stress fractures occurring in élite female athletes are related to reductions in bone mineral density (BMD), we measured BMD in nine athletes with such fractures and nine athletes without fractures who were matched for age, weight, height and sport. BMD was measured in three regions: upper limbs (distal radius), axial skeleton (lumbar spine) and lower limbs (femoral neck) by photon absorptiometry. The number of menses per year was significantly less (P less than 0.04) and the age of menarche was significantly delayed (mean +/- SD; 16.1 +/- 0.4 v. 14.4 +/- 1.5 years, P less than 0.02) in the fracture group compared with the non-fracture group. There was no significant difference in BMD between the two groups at any of the measurement sites. Moreover although the fractures occurred mainly in lower limb bones, at sites characterised by predominantly cortical bone, all athletes had femoral neck BMD values within the 95% confidence limits for normal non-athletic women. We conclude that stress fractures in élite female athletes are largely independent of BMD.     

COMPARISON OF BONE MASS IN FOREARM, LUMBAR VERTEBRA AND HIP BY SINGLE AND/OR DUAL ENERGY X-RAY ABSORPTIOMETRY

Objective. Bone mineral density(BMD) was measured in normal Chinese women with single X-ray sbsorptiometry (SXA) and dtml energy X-ray absorptiometry (DEXA). These two methods were compared to evaluate the sensitivity in reflecting bone loss. Methods. Measurements were performed in 300 women aged 20～79(5 for each age). The 8mm distal,1/4 distal, ultra distal site ot the nondominant torearrn were measured with SXA; the AP spine(L2-4), right femoral neck(Neck), Ward‘s triangle(Ward)and trochantor (Troch) were measured with DEXA. Ten wcanen had 5 repeated measurements to evaluate the reproducibility ot the equipments. Rgsults. The BMD peak for Neck and Ward was found between age 20～29, for ultra distal and L2-4 at age 30～39, for 8mm distal, 1/4 distal and Troth at age 40～49. After reaching the peak values, BMD at all sites decreased with increasing age. The highest rate of yearly loss of BMD was at 8ram distal. Resuits of measurement showed siguifieant correlation between SXA and DEXA. Comparing with premenopausal women, the bone loss rate in postnaenopausal women was faster and highest up to 11～15 years and the highest rate was at the Ward. Conclusion. The present study gives the normal values of BMD at seven sites for nccmal Chinese wonaen. The ages of BMD peak were different at seven sites. The yearly loss of BMD was highest at 8ram distal, it seemed to be the sensitive region for bone loss with age. The postmenopansal women had highly signiflcantly loss bone mass than the premenopausal women, Comparing with premenopause, postmenopsuse BMD for Ward‘s triangle with DEXA seemed to be most sensitive to the effect of menopause.     

Assessment of serial changes of bone mineral density at lumbar spine and femoral neck before and after liver transplantation

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Predictors of bone mass in healthy older men in the community

OBJECTIVES: To determine the predictors of bone mass (in lumbar spine and femoral neck) in healthy older men living in the community. DESIGN: Cross-sectional study. Bone mineral density (BMD) was measured and known predictors of bone mass and bone turnover were assessed. SUBJECTS AND SETTING: 113 independent, healthy men (70 years and over), not taking glucocorticoid therapy and without medical conditions known to affect bone mass and bone turnover, were recruited from recreational and sports clubs in southern Sydney in April/May 1997. MAIN OUTCOME MEASURES: BMD (measured by dual-energy x-ray absorptiometry); known predictors of bone mass (height; weight; body mass index; calcium level; serum 25-hydroxyvitamin D and free testosterone levels); and markers of bone turnover (serum bone Gla protein and procollagen-1 concentrations, and urinary deoxypyridinoline excretion rates). RESULTS: The mean age of the men was 76.6 years (range, 70-92 years). Mean (SE) BMD of the lumbar spine was 1.143 (0.019) g/cm2 and that of the femoral neck was 0.897 (0.013) g/cm2. BMD values indicating osteoporosis were found in the lumbar spine in 13 men (11.5%) and in the femoral neck in 35 men (31%). The best predictor of lumbar spine BMD was weight (R = 0.37; P = 0.001), and weight- and age-predicted femoral-neck BMD (R = 0.49; P < 0.001). The study group was analysed in two groups with BMD higher or lower than median bone mass corrected for age. Men with lower femoral-neck BMD for age had significantly lower weight, lower lean mass and higher bone Gla protein concentrations. In addition, men with lower lumbar spine BMD for age also had significantly lower fat mass. CONCLUSIONS: These data indicate that measures of body composition, such as weight and lean mass, are the main predictors of bone mass in healthy, community-dwelling older men.