宫廷理筋手法治疗纤维肌痛综合征临床观察

目的 观察宫廷理筋手法治疗纤维肌痛的疗效。方法 应用宫廷理筋手法对30例纤维肌痛患者进行6周的治疗,采用纤维肌痛影响量表（fibromyalgia impact questionnaire, FIQ）测评患者的生活质量,采用数字分级量表（numerical rating scale, NRS）和压痛点数评价疼痛的变化情况。结果 与治疗前比较,治疗后患者的FIQ和NRS评分均显著降低,压痛点数显著减少,差异有统计学意义（P＜0.05）。结论 宫廷理筋手法对纤维肌痛综合征有显著的临床疗效。     

手法联合盐酸氨基葡萄糖治疗膝骨关节炎临床观察

目的 基于红外热成像技术评价手法治疗膝骨关节炎(knee osteoarthritis,KOA)的临床疗效,探讨手法作用机制,形成手法治疗KOA规范化诊疗评估方案。方法 通过简单随机方法将40例KOA患者分为对照组和治疗组,每组20例（治疗组和对照组分别被剔除1例和2例）。治疗组采用手法治疗联合盐酸氨基葡萄糖胶囊口服,对照组采用自我按摩联合盐酸氨基葡萄糖胶囊口服。治疗2周后,以膝关节区域温度、督脉温度、视觉模拟量表（visual analogue scale, VAS）评分、西安大略和麦克马斯特大学骨关节炎指数（the Western Ontario and McMaster Universities Osteoarthritis Index,WOMAC）、连续5次坐立时间、15 m直线连续行走时间为指标,评价两组疗效。结果 两组治疗后VAS评分均显著降低（P＜0.05）;治疗组治疗前后VAS评分差值显著大于对照组（P＜0.05）。两组治疗后WOMAC的疼痛、僵硬、生活功能评分和总分显著降低（P＜0.05）;其中治疗组治疗前后WOMAC总分和僵硬评分差值显著大于对照组（P＜0.05）。两组治疗后连续5次坐立时间、15 m直线连续行走时间均显著降低（P＜0.05);治疗组治疗后连续5次坐立时间、15 m直线连续行走时间降低值显著大于对照组（P＜0.05）。两组治疗后膝关节区域温度显著降低（P＜0.05）,督脉区域温度显著升高(P＜0.05);治疗组治疗后膝关节区域温度降低值和督脉区域温度升高值显著大于对照组（P＜0.05)。两组基于WOMAC总分的临床疗效比较,差异有统计学意义（P＜0.05）,治疗组临床疗效明显优于对照组。结论 中医手法治疗可减轻KOA患者膝关节疼痛、僵硬症状,改善日常生活功能,疗效优于自我按摩。     

手法结合肌力训练治疗膝骨关节炎的临床疗效观察

目的 观察手法结合肌力训练治疗膝骨关节炎（KOA）的疗效。方法 将在我院就诊的120例KOA患者随机分为观察组与对照组各60例，观察组行手法结合肌力训练治疗，对照组行玻璃酸钠关节内注射治疗。评定两组临床疗效，统计分析两组治疗前和治疗5周后视觉模拟评分（VAS）、西安大略和麦克马斯特大学骨关节炎指数评分（WOMAC）、血清炎性因子肿瘤坏死因子-α（TNF-α）、白细胞介素-1 β（IL-1β ）含量和远红外热像图（IRT）。结果 治疗后，观察组总有效率高于对照组（ P<0.05）；观察组VAS评分、WOMAC评分、血清炎性因子TNF-α、IL-1β 含量均低于对照组（ P<0.05）；治疗前两组典型病例膝关节IRT均表现不同程度的低温反应，治疗后，膝关节IRT色域均趋暖。结论 手法结合肌力训练能改善KOA患者膝关节的功能活动，减轻疼痛症状，对KOA有一定的防治作用，是一种行之有效且安全简便的防治一体化方案。     

理筋正骨手法联合易化牵伸术治疗陈旧性踝关节扭伤30例

目的 观察中医理筋正骨手法联合易化牵伸术治疗陈旧性踝关节扭伤的疗效。方法 采用随机对照的方法,将60例陈旧性踝关节扭伤患者随机分为治疗组和对照组,每组30例,治疗组采用理筋正骨手法联合易化牵伸术治疗,对照组采用电磁波治疗仪照射和针刺治疗,治疗2个疗程后分别比较患者Baird- Jackson踝关节功能评分、疼痛视觉模拟评分（visual analogue scale,VAS）以及压痛程度评分的差异。结果 治疗后2组患者VAS评分、压痛程度评分、Baird- Jackson踝关节功能评分较本组治疗前显著改善（P＜0.05）,且治疗组各评价指标改善程度显著大于对照组（P＜0.05）。结论 理筋正骨手法联合易化牵伸术可以改善陈旧性踝关节扭伤患者症状,提高临床疗效。     

小针刀配合超短波、关节松动术治疗肩周炎的临床观察

目的 观察小针刀配合超短波、关节松动术的治疗作用.方法 将118例患者随机分为治疗组和对照组,对照组进行超短波治疗,治疗组采用小针刀配合超短波、关节松动术治疗,21天后对比疗效.结果 两组患者临床疗效比较,治疗组愈显率明显高于对照组;治疗组与对照组疼痛视觉模拟评分法（VAS）评分比较,治疗后治疗组VAS减低,明显低于对照组（P＜0.01）.结论 针刀配合超短波、关节松动术治疗肩周炎比单独采用超短波治疗效果显著.     

针灸辨证施治配合关节松动术治疗冻结肩80例疗效观察

目的：对照观察针灸辨证施治配合关节松动术对冻结肩的临床疗效。方法：将80例冻结肩患者随机分为治疗组和对照组。治疗组用针灸辨证施治配合关节松动术治疗，对照组只用针灸辨证施治。治疗前、后进行肩关节活动度（ROM）和肩痛症状（VAS）评估。结果：两组患者治疗前后肩关节活动度（ROM）和肩痛症状（VAS）较治疗前差异有显著性意义（P〈0．01），两组间肩关节活动度（ROM）和肩痛症状（VAS）比较差异有显著性意义（P〈0．01），治疗组优于对照组。结论：针灸辨证施治配合关节松动术治疗冻结肩的疗效优于单纯采用针灸辨证施治。     

针刺配合关节松动术治疗肩关节周围炎40例

目的观察针刺法配合关节松动术治疗肩关节周围炎的疗效。方法将160例肩关节周围炎患者分为四组,每组40例。针刺组患者给予选穴针刺疗法,关节松动术组给予关节松动术治疗,每日1次,每次40min;针刺＋关节松动组,同时给予针刺及关节松动治疗,对照组予常规疗法。治疗8周后,评定疗效并进行组间比较。结果有效率依次为针刺＋关节松动术组（90%）、关节松动组（85%）、针刺组（82.5%）、对照组（70%）,与对照组比较均有统计学意义（P〈0.05）。结论针刺结合关节松动术治疗肩关节周围炎安全有效,无不良反应。     

关节松动术结合物理治疗对腰椎间盘突出症的临床观察

目的:探讨关节松动术结合物理治疗腰椎间盘突出症的临床应用和治疗效果。方法:将40例腰椎间盘突出症患者随机分为治疗组和对照组,治疗组采用关节松动术结合干扰电、牵引、超短波方法进行治疗;对照组采用传统按摩手法配合牵引治疗。治疗前后评定患者的临床疗效,观察两组患者治疗后评定分值。结果:两组临床治愈率较佳,两组治愈率比较,P>0.05;有效率比较,P>0.05;治疗前后疼痛改善程度比较,P<0.001。结论:关节松动术结合物理治疗对腰椎间盘突出症患者在临床症状改善、疼痛减轻方面有满意的临床疗效。     

关节松动术结合物理治疗对腰椎间盘突出症的临床观察

目的：探讨关节松动术结合物理治疗腰椎间盘突出症的临床应用和治疗效果。方法：将40例腰椎间盘突出症患者随机分为治疗组和对照组,治疗组采用关节松动术结合干扰电、牵引、超短波方法进行治疗;对照组采用传统按摩手法配合牵引治疗。治疗前后评定患者的临床疗效,观察两组患者治疗后评定分值。结果：两组临床治愈率较佳,两组治愈率比较,P〉0.05;有效率比较,P〉0.05;治疗前后疼痛改善程度比较,P〈0.001。结论：关节松动术结合物理治疗对腰椎间盘突出症患者在临床症状改善、疼痛减轻方面有满意的临床疗效。     

中医手法松动术治疗术后骨关节僵硬疗效观察分析

目的观察分析术后骨关节僵硬患者采取中医手法松动术治疗的临床效果。方法择取2016年6月至2018年6月我院收治的76名术后骨关节僵硬患者开展研究,将其随机划分为对照组(n=38)和观察组(n=38),对照组人员采取一般治疗,观察组添加中医手法松动术治疗。对入选患者治疗效果及关节活动情况开展对比研究。结果观察组治疗有效率及关节活动情况均优于对照组,差异显著(P0.05)。结论术后骨关节僵硬患者采取中医手法松动术治疗效果显著,可改善患者关节活动情况,对患者恢复有积极作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.