芦荟多糖对动物实验性胃溃疡的影响

目的研究芦荟多糖对动物实验性胃溃疡的影响。方法应用大鼠幽门结扎型胃溃疡模型、大鼠醋酸烧灼型胃溃疡模型、小鼠利血平致胃溃疡模型,观察芦荟多糖ig给药对实验性胃溃疡的预防与治疗作用及对大鼠胃液、胃酸、胃黏液、胃蛋白酶分泌及活性的影响。结果芦荟多糖对实验性胃溃疡具有明显的预防及治疗作用,并能抑制胃液分泌、胃酸分泌及减少胃蛋白酶、胃黏液的分泌,但对胃蛋白酶活性无明显影响。结论芦荟多糖对动物实验性胃溃疡具有良好的预防及治疗作用,并能抑制溃疡致病因子胃酸及胃蛋白酶的分泌。     

芩连合剂对实验性胃溃疡大鼠胃黏膜保护作用的机制研究

目的探讨芩连合剂(QLHJ)对急性胃溃疡大鼠胃黏膜的保护作用及其作用机制.方法采用无水乙醇灌胃法复制大鼠急性胃溃疡模型,测定胃黏膜细胞内丙二醛(MDA)、超氧化物歧化酶(SOD)的表达、谷胱苷肽过氧化物酶(GSH-Px)活力及钙离子浓度.结果模型组大鼠胃黏膜细胞内钙离子浓度较其他各组明显升高,与芩连合剂组和正常组比较有非常显著性差异(P＜0.01);SOD、GSH-Px的表达较其他各组明显降低,MDA表达较其他各组明显升高,与芩连合剂组、地尔硫组和正常组比较有显著性差异(P＜0.05,P＜0.01).结论QLHJ通过降低胃黏膜细胞内钙离子浓度的水平,降低胃黏膜细胞内MDA的表达和提高胃黏膜细胞内SOD和GSH-Px的表达,从而发挥其保护胃溃疡大鼠胃黏膜细胞的作用.     

平溃散对急性胃溃疡及胃黏膜损伤的保护作用

目的:研究平溃散(绞股蓝,沙棘等)对抗急性溃疡的作用.方法:采用组织胺和消炎痛法建立大鼠实验模型,测量溃疡面积和胃黏液的量.结果:平溃散对组胺引起的急性溃疡有一定的抑制作用,对消炎痛引起的胃黏膜分泌的减少有一定的抑制作用.结论:平溃散具有抗溃疡、保护胃黏膜的作用.     

Cytoprotective effects of Bergenia ciliata Sternb, extract on gastric ulcer in rats

Bergenia ciliata is used for the treatment of stomach disorders in the folk medicine of some areas of South East Asia. This study was designed to evaluate its gastroprotective effects on ethanol/HCl, indomethacin and pylorus ligation-induced gastric ulcers in rats. Doses of 15, 30 and 60 mg/kg b/w of the aqueous and methanol extracts of the rhizome were administered 1 h after ulcerogenic treatment. The animals were killed 3 h later, their stomachs removed and the mean area of ulcer lesion was determined. The weight of mucus and gastric acidity were also measured. The aqueous extract decreased the ulcer lesion (p < 0.05) in all models to a greater extent than the methanol extract, but at the higher doses the effect was reduced. In addition, the antiulcer activity appears to be mediated via cytoprotective effects conferred by enhancement of the mucosal barrier, rather than by prevention of gastric acid secretion or the lowering of pH and acidity.     

胃溃宁抗胃溃疡的研究

目的:研究胃溃宁的抗胃溃疡及抑酸作用。方法:分别采用皮下注射利血平法、shay氏结扎幽门法、乙酸烧灼法等建立大鼠胃溃疡模型,随机分组,模型对照组灌胃给予0.5%羧甲基纤维素钠10ml/kg、药物治疗组分别灌胃给予雷尼替丁50mg/kg、胃康胶囊1.8g/kg和不同剂量的胃溃宁(4.0g/kg,2.0g/kg,1.0g/kg),观察胃溃宁对大鼠胃溃疡面积、胃液分泌量、胃液总酸度及胃蛋白酶活性的影响。结果:胃溃宁4.0g/kg,2.0g/kg,1.0g/kg能明显抑制各型胃溃疡的形成,并有效减小胃溃疡面积,与模型对照组比较,有显著性差异(P<0.01或P<0.05);胃溃宁4.0g/kg,2.0g/kg对于幽门结扎型溃疡,能有效减少胃液分泌量,降低胃液分泌总酸度,抑制胃蛋白酶活性,与模型对照组比较,有明显差异(P<0.01或P<0.05)。结论:胃溃宁对大鼠胃溃疡模型有明显的抗胃溃疡作用。     

Studies on the antiulcer activity of Moringa oleifera leaf extract on gastric ulcer models in rats

The methanol fraction of M. oleifera leaf extract was found to possess significant protective actions in acetylsalicylic acid, serotonin and indomethacin induced gastric lesions in experimental rats. A significant enhancement of the healing process in acetic acid—induced chronic gastric lesions was also observed with the extract-treated animals.     

dl-四氢巴马汀抗大鼠胃溃疡作用

 目的研究dl-四氢巴马汀(dl-THP)对大鼠急、慢性溃疡的保护作用,并初步分析其机制。方法胃黏膜损伤由吲哚美辛、束缚应激、结扎幽门和胃浆膜下注入醋酸引起。dl-THP15,30,45mg·kg^-1灌胃,1日2次,共5次。测定大鼠胃溃疡数目、面积、胃酸分泌、胃蛋白酶活性以及胃黏膜血流。结果dl-THP15,30,45mg·kg^-1能抑制大鼠胃黏膜损伤,且呈剂量依赖性;dl-THP30mg·kg^-1对大鼠慢性胃溃疡的抑制率为75.8%,与雷尼替丁30mg·kg^-1的67.7%相仿。dl-THP可增加胃黏膜血流,但对胃酸和胃蛋白酶分泌无影响。结论dl-THP具有明显抗实验性胃溃疡作用,可能与其增加胃黏膜血流有关,亦可能部分通过阻滞胃黏膜DA受体而发挥作用。     

Quercetin and naringenin; effects on ulcer formation and gastric secretion in rats

The antiulcerogenic effect of two flavonoids, quercetin and naringenin, in acute gastric ulcer (cold-restraint and pylorus-ligated) has been investigated. In both models quercetin and naringenin presented a significant decrease in ulcer index with respect to the control group. Nevertheless, in the Shay-ligated method no decrease in either volume, acidity or pepsin were observed, however, a significant difference was found in histamine secretion values.     

铝碳酸镁合奥美拉唑治疗胃溃疡49例临床疗效观察

目的：探讨铝碳酸镁合奥美拉唑治疗胃溃疡的临床疗效。方法：将98例胃溃疡患者随机分为奥美拉唑治疗组（对照组）和铝碳酸镁合奥美拉唑治疗组（观察组）各49例,比较两组患者的临床疗效。结果：观察组患者治疗总有效率为91．8％,显著高于对照组患者的75．5％,组间差异有统计学意义（P〈0．05）。结论：铝碳酸镁合奥美拉唑治疗胃溃疡临床疗效较好,值得临床推广应用。     

胃疡宁丸抗实验性胃溃疡的药效及机制研究

目的:研究胃疡宁丸抗实验性胃溃疡的药效及其机制.方法:采用大鼠醋酸注射法、水浸应激性、阿司匹林诱发及幽门结扎性胃溃疡模型,观察胃疡宁丸抗胃溃疡的作用并考察其对血清NO,EGF含量,胃黏膜血流量,PGE2含量,及对胃液分泌、胃酸含量、胃蛋白酶活性的影响.结果:胃疡宁丸能够降低各种胃溃疡模型的溃疡指数,升高NO,EGF含量,PGE2含量及胃黏膜血流量,抑制胃液分泌和胃酸含量及降低胃蛋白酶活性.结论:胃疡宁丸具有明显的抗实验性胃溃疡的作用,该作用与其减少胃黏膜损伤因子(胃酸、胃蛋白酶),增加胃黏膜屏障的功能,增强胃黏膜修复作用(NO,EGF,PGE2,胃黏膜血流量)有关.     

复方丹蒲汤抗实验性胃溃疡的作用

目的:研究复方丹蒲汤(丹参、蒲公英、砂仁等)抗实验性胃溃疡的作用及其机制。方法:采用小鼠水浸应激性、利血平性、无水乙醇性、幽门结扎性胃溃疡模型观察复方丹蒲汤对胃溃疡的保护作用及对大鼠胃液分泌的影响;采用小鼠胃排空方法观察复方丹蒲汤对胃运动的影响;以热板法观察其镇痛作用。结果:复方丹蒲汤对小鼠水浸应激性、利血平性及无水乙醇性胃溃疡模型均有明显的保护作用;能抑制大鼠胃酸及胃蛋白酶的分泌,降低大鼠胃溃疡指数,减少溃疡面积;抑制小鼠胃排空;延长小鼠热刺激的痛反应时。结论:复方丹蒲汤具有抗实验性胃溃疡的作用。该作用可能与其抑制胃液分泌、胃平滑肌运动及镇痛作用有关。     

水黄皮根抗实验性胃溃疡活性部位的筛选研究

目的：对水黄皮根抗实验性胃溃疡的有效部位进行筛选。方法：采用大鼠无水乙醇胃黏膜损伤模型和小鼠利血平型胃溃疡模型,观察水黄皮根乙醇总提取物和不同极性萃取部位对胃黏膜的影响。结果：水黄皮根乙醇提取物、醋酸乙酯和正丁醇萃取部位都能明显抑制无水乙醇和利血平对胃黏膜的损伤。其对无水乙醇模型的损伤抑制率分别是86.4%,85.4%,11.5%,对利血平模型的溃疡抑制率分别是37.8%,33.8%,19.7%。结论：水黄皮根乙醇提取物、醋酸乙酯和正丁醇萃取物具有明显抗实验性胃溃疡作用,作用最强的部位是醋酸乙酯萃取部位。     

白芨多糖对动物实验性胃溃疡的影响

目的观察白芨多糖对实验性小鼠和大鼠胃黏膜损伤的影响。方法采用水提醇沉的方法制备白芨多糖。采用冷水应激、乙醇诱导法制作小鼠胃溃疡模型,幽门结扎等方法制作大鼠胃溃疡模型。试验动物各分为5组:白芨多糖低剂量组、白芨多糖中剂量组、白芨多糖高剂量组、对照组和模型组,连续给药3 d,造模后处死动物并观察胃溃疡指数。结果白芨多糖对冷水应激小鼠胃黏膜损伤、乙醇诱导的小鼠胃黏膜损伤及结扎型大鼠胃黏膜损伤均具有保护作用。结论白芨多糖对实验性动物胃溃疡具有保护作用。     

云母对实验性胃溃疡愈合质量的影响

目的:探讨云母对实验性胃溃疡愈合质量的影响。方法:建立实验性醋酸致大鼠胃溃疡模型,成模后分组给药治疗14 d,取其标本,评估再生黏膜组织学及功能成熟度,以判定溃疡愈合质量。结果:经云母治疗后,大鼠再生黏膜厚度增加、囊状扩张腺体数目减少,肉芽组织炎症细胞浸润数量减少、微血管数量增加、胶原面积增加,黏膜中性黏液和氨基己糖分泌增多,血浆PGE₂,EGF分泌增多,免疫组化染色中黏膜局部EGFR,bFGF阳性细胞表达增强。结论:云母能提高再生黏膜组织学及功能成熟度,最终达到高质量的溃疡愈合。     

胃液表皮生长因子在胃溃疡愈合中的作用

目的　探讨人胃溃疡愈合过程中表皮生长因子 (EGF)含量的变化及其作用。方法　采用放免的方法 ,对正常胃粘膜 (8例 )、胃溃疡活动期 (GA组 10例 )、愈合期 (GH组 10例 )、瘢痕期 (GS组 10例 )胃液的EGF含量的进行测定。结果　正常胃粘膜的胃液EGF的含量为 (5 0 2 .6± 5 1.3)pg/ml(n =8) ,GA组胃液EGF含量为 (15 6 .2± 4 6 .7)pg/ml(n =10 ) ,经统计学处理差异有显著性意义 (P <0 .0 5 ) ;而GH和GS组胃液的EGF含量分别为 (386 .1± 76 .8)pg/ml(n =10 )、(4 6 6 .3± 95 .4 )pg/ml(n =10 ) ,与GA组比较 ,差异有显著性意义(P <0 .0 5 )。结论　胃液EGF与溃疡的发生和愈合有关。     

野甘草乙醇提取物治疗大鼠胃溃疡作用的研究

目的研究野甘草乙醇提取物对胃溃疡的治疗作用。方法通过醋酸烧灼法制成大鼠胃溃疡模型,按照同窝同性、体质量配对分为2组,实验组每只以野甘草乙醇提取物1 g/(kg.d)喂养,共喂30 d;对照组不喂药。30 d后剖腹取胃,测定溃疡体积。结果实验组大鼠胃溃疡体积小于对照组(P<0.05)。结论野甘草乙醇提取物对大鼠胃溃疡有治疗作用,应对其药理作用进行更深入的研究。     

健胃愈疡颗粒对胃溃疡患者胃黏膜hTFF1mRNA表达的影响

目的　探讨健胃愈疡颗粒 (JWYY)对胃溃疡 (GU)患者胃黏膜hTFF1mRNA表达的影响 ,揭示其抗溃疡复发的分子机制。方法　将 60例胃镜下确诊的GU (中医辨证为肝郁脾虚证 )患者 ,随机分为JWYY治疗组和西药对照组 ,每组 3 0例 ;另设胃镜下无胃黏膜病变的患者 10例为正常组。两组GU患者第 1个疗程结束前后均于胃镜下取溃疡边缘组织活检标本 2块 ,迅速置液氮 -80℃保存并行RT -PCR检测。结果　JWYY能提高人胃黏膜hTFF1mRNA表达 ,两组经统计学处理无显著性差异。结论　JWYY能增强人胃黏膜hTFF1mRNA的上增性表达 ,从而提高溃疡愈合质量 ,是JWYY抗胃溃疡复发作用的部分机制     

Gastroprotective effects of the essential oil of Hyptis crenata Pohl ex Benth. on gastric ulcer models

Ethnopharmacological relevance

Hyptis crenata Pohl ex Benth (Lamiaceae), popularly known as “hortelã-brava” or “hortelã do campo”, is widely distributed in the northeast of Brazil. In Brazil, the leaves of this plant have been used in traditional medicine for the treatment of gastrointestinal disturbances, including gastric ulcers. In an attempt to experimentally validate this claimed antiulcerogenic activity, the gastroprotective effects of the essential oil extracted from the leaves of the Hyptis crenata Pohl ex Benth (EOHc) were evaluated in recognized gastric ulcer models in mice.

Materials and methods

EOHc was obtained by hydrodistillation and analyzed using gas chromatography–mass spectrometry (GC–MS). Swiss male mice (25–30 g) were used for the studies. The gastric ulcers were induced by oral administration of absolute ethanol or indomethacin 45 min after oral pretreatment with EOHc, vehicle and positive control drugs. One hour after the ulcerative challenges, the stomachs were removed and the area of the lesions was measured. The volume, pH and total acidity of the gastric secretions were determined using the pylorus ligature model. The gastrointestinal motility was measured using gastric emptying and intestinal transit. The ethanol-induced gastric mucus depletion and lipid peroxidation were also analyzed.

Results

Our findings are as follows: A significant inhibition of gastric lesions induced by absolute ethanol was observed in the mice pre-treated with EOHc, at a dose of 30 and 100 and 300 mg/kg (5.56±1.51, 2.88±0.82 and 1.71±0.54 mm², respectively) compared to control group (118.03±35.4 mm²). Also, EOHc (300 mg/kg) produced a gastroprotective effect against the gastric lesions induced by indomethacin (16.07±4.68 mm²) compared to control group (38.64±6.1 mm²). EOHc pretreatment produced a reduction in the ethanol-induced lipid peroxidation from 3.9±0.22 to 2.4±0.1 μmol/mg tissue (EOHc-300 mg/kg and control group, respectively). We also observed that EOHc pretreatment decreased the gastric emptying, but did not alter the intestinal transit ratio, ethanol-induced depletion of the gastric wall mucus or secretion parameters (volume, pH and [H⁺]).

Conclusion

Our data indicate that EOHc exerts a gastroprotective effect, indicated by its significant inhibition of gastric lesions in ethanol- and indomethacin-induced ulcer models, which may be associated with its accelerating effect on gastric emptying and reduction in oxidative damages. Our data suggest a potential therapeutic application for EOHc in the treatment of gastric ulcers.     

大黄黄连泻心汤抑制NLRP3炎症小体活性对急性胃溃疡的保护作用及机制研究

[目的] 研究不同浸渍条件下大黄黄连泻心汤抑制 NOD 样受体热蛋白结构域相关蛋白 3（NLRP3）炎症小体活性对急性胃溃疡小鼠的保护作用,探讨其作用机制并筛选最佳浸渍条件。[方法] 对小鼠进行无水乙醇灌胃以建立急性胃溃疡模型,并给予不同浸提条件下的大黄黄连泻心汤进行实验,同时选用 MCC950 抑制剂作为阳性药。对各组小鼠进行胃组织苏木精-伊红（HE）染色,观察胃组织病理变化;统计溃疡指数（UI）;用酸碱度（pH）试纸测试胃液 pH值;检测胃液胃蛋白酶活性（PA）;采用酶联免疫吸附（ELISA）法检测血清中白细胞介素 1β（IL-1β）和白介素 18（IL-18）的水平;采用蛋白免疫印迹法（Western blot）检测药物组小鼠胃组织中 NLRP3、半胱氨酸蛋白酶-1（caspase-1）的蛋白表达。[结果] 与空白组相比,模型组小鼠胃组织出现明显的溃疡病变,各项指标异常。与模型组相比,药物组可降低乙醇诱导下急性胃溃疡模型小鼠的溃疡指数（P＜0.05 或 P＜0.01）,抑制胃酸的分泌（P＜0.05）,抑制胃蛋白酶活性（P＜0.01）;各药物组中促炎细胞因子 IL-1β、IL-18 的表达明显降低（P＜0.01）;通过各药物组灰度值/对照模型组蛋白灰度值的比较,表明药物组能够降低 NLRP3、Caspase-1 蛋白表达（P＜0.01 或 P＜0.05）。最后,对不同浸渍条件下大黄黄连泻心汤的药效进行综合评分,结果表明 E（85 ℃、15 min）组评分最高。[结论] 大黄黄连泻心汤对无水乙醇灌胃诱导的急性胃溃疡小鼠有保护作用,其机制可能与抑制 NLRP3 炎症小体活性有关。通过各药物组抑制 NLRP3 炎症小体的表达差异,得出 85 ℃、15 min 的最佳浸渍条件。     

吴茱萸抗大鼠应激性胃溃疡研究

[目的]研究吴茱萸对大鼠应激性胃溃疡的作用及其可能的机制。[方法]实验分为空白对照组、水浸-束缚应激性(WRS)胃溃疡模型组及模型加吴茱萸生药0.45 g/kg体质量组、0.9 g/kg体质量组和1.8 g/kg体质量组,计算各组大鼠胃溃疡指数;采用高效液相色谱-电化学法(HPLC-ECD)检测大鼠血清肾上腺素(E)水平。[结果]吴茱萸0.45 g/kg组能显著降低溃疡指数(P0.05)抑制溃疡形成。与模型组相比吴茱萸给药组均能显著抑制血清中E含量(P0.05)。[结论]吴茱萸具有抗大鼠应激性胃溃疡的作用,其作用机制可能与调节血清中E含量有关。