八正合剂抗感染作用的实验研究

目的　探讨八正合剂治疗泌尿系统感染性疾病的药理学作用基础。方法　将大肠杆菌注入小鼠膀胱后观察药物对细菌上行感染肾脏的抑制作用 ;家兔 iv大肠杆菌内毒素后观察药物对细菌内毒素引起机体发热的影响 ;采用大鼠足跖 sc角叉菜胶所致炎性肿胀模型观察药物对炎性肿胀及机械压迫足跖疼痛的抑制作用。结果　八正合剂 ig给药可显著降低大肠杆菌上行感染肾脏的带菌剖面百分率 [ED50 为 (11.0 1± 1.6 3) g/ kg,95 %可信限为9.5 0～ 12 .76 g/ kg],抑制大肠杆菌内毒素诱发家兔体温升高 ,减轻角叉菜胶所致大鼠足跖炎性肿胀及致炎后肿胀部位机械压迫痛阈降低程度。结论　八正合剂治疗泌尿系统感染性疾病的作用机制主要与其清除尿路细菌和减轻发热、肿胀、疼痛等炎症反应症状有关。     

复方黄芪合剂对小鼠免疫功能影响

目的:观察复方黄芪合剂对小鼠免疫功能的影响。方法:将实验小鼠随机分为复方黄芪合剂高剂量组(24 g·kg-1)、复方黄芪合剂低剂量组(12 g·kg-1)、香菇菌多糖片组(0.03 g·kg-1)和蒸馏水对照组,通过测定药物对小鼠的胸腺和脾脏指数、碳粒廓清吞噬指数(K)及校正吞噬指数(α)和血清溶血素抗体积数的影响,观察复方黄芪合剂对机体免疫功能的影响。结果:复方黄芪合剂可增强小鼠的脾脏指数(P0.05)及胸腺指数(P0.01),升高碳粒廓清吞噬指数(P0.01)及校正吞噬指数(P0.01),提高血清溶血素抗体积数(P0.05,P0.05)。结论:复方黄芪合剂对小鼠的特异性和非特异性免疫功能有促进作用。     

复方黄芪合剂对小鼠免疫功能影响简

目的：观察复方黄芪合剂对小鼠免疫功能的影响。方法：将实验小鼠随机分为复方黄芪合剂高剂量组（24g·kg-1）、复方黄芪合剂低剂量组（12g·kg-1）、香菇茵多糖片组（0．03g·kg-1）和蒸馏水对照组,通过测定药物对小鼠的胸腺和脾脏指数、碳粒廓清吞噬指数（K）及校正吞噬指数（d）和血清溶血素抗体积数的影响,观察复方黄芪合剂对机体免疫功能的影响。结果：复方黄芪合剂可增强小鼠的脾脏指数（P〈O．05）及胸腺指数（P〈0．01）．升高碳粒廓清吞噬指数（P〈0．01）及校正吞噬指数（P〈0．01）,提高血清溶血素抗体积数（P〈0．05,P〈0．05）。结论：复方黄芪合剂对小鼠的特异性和非特异性免疫功能有促进作用。     

芝莲化癞颗粒对荷瘤小鼠的免疫功能效应研究

目的：对芝莲化瘢颗粒影响荷瘤小鼠的免疫功能进行科学的实验研究,为确定该制剂的功能主治和临床疗效提供依据。方法：制备H22肝癌实体瘤荷瘤小鼠模型,选取主要指标：巨噬细胞吞噬百分率、吞噬指数,溶血素、溶血空斑的形成,外周血淋巴细胞转化率,进行各项对比实验。结果：芝莲化瘢颗粒以30g·kg-1、15g·kg-1、7．5g·kg-1的剂量给荷瘤小鼠连续10天灌服,中剂量组可显著提高荷瘤小鼠腹腔巨噬细胞对鸡红细胞的吞噬百分率和吞噬指数,大剂量组可显著促进荷瘤小鼠溶血素及溶血空斑的形成,可显著提高荷瘤小鼠外周血淋巴细胞转化率。结论：芝莲化瘢颗粒能够显著提高感染H22肝癌实体瘤小鼠的肌体免疫力,其功能主治项可以描述为：能够显著提高肌体免疫力,缓解肝细胞癌对肝脏的损害,延长生存期。     

HG颗粒对免疫功能低下小鼠单核巨噬细胞吞噬功能的影响

目的:研究HG颗粒对免疫功能低下小鼠单核巨噬细胞吞噬功能的影响.方法:皮下注射环磷酰胺建立免疫功能低下小鼠模型,分别予HG颗粒、盐酸左旋咪唑片灌胃治疗.对照观察药物对免疫功能低下小鼠单核巨噬细胞吞噬刚果红染料能力的影响.结果:免疫功能低下小鼠吞噬刚果红染料能力显著降低.经HG颗粒治疗后有明显提高,综合疗效优于阳性药物组.结论:HG颗粒能显著提高免疫功能低下小鼠单核巨噬细胞吞噬能力.     

脾虚小鼠的吞噬细胞功能状态及健脾合剂对其的影响

为了观察健脾合剂对脾脾虚小鼠吞噬细胞功能的影响。测定了脾虚模型小鼠在造模和健合剂治疗后，免疫器官重量和吞噬细胞功能的变化。结果表明，与正常小鼠比较，脾虚小鼠脾、胸腺重量以及全血白细胞、腹腔巨噬细胞的吞噬功能显著低下；经健脾合剂治疗后，上述变化可得到纠正而趋于正常。说明健脾合剂具有提高机体免疫功能的作用。本实验亦验证中医脾的发生与免疫功能低下有着密切关系。     

小儿复感宁合剂增强机体免疫功能的实验研究

目的:探讨小儿复感宁合剂对机体免疫功能的影响。方法:将50只小鼠随机分为5组,A,B组灌蒸馏水,C组予贞芪扶正胶囊,D,E组予小儿复感宁合剂。给药第8 d皮下注射环磷酰胺造模,分别观察小儿复感宁合剂对小鼠腹腔巨噬细胞吞噬功能、2,4-二硝基氯苯(DNCB)所致迟发性超敏反应、鸡红细胞(CRBC)致敏小鼠溶血素抗体生成的影响。结果:小儿复感宁合剂能增强小鼠腹腔巨噬细胞吞噬功能;增强DNCB所致迟发性超敏反应;提高小鼠血清溶血素抗体水平。结论:小儿复感宁合剂能增强环磷酰胺所致免疫功能低下小鼠非特异性免疫功能和特异性免疫功能。     

蒲公英提取物对小鼠免疫功能的调节作用

目的:观察蒲公英提取物(ETM)对小鼠免疫功能的调节作用。方法:各组小鼠分别经口给予0.6、1.2、3.6g/(kg·d)的ETM及灭菌水30天后,观察其体重、免疫器官指数、脾淋巴细胞增殖能力、自然杀伤细胞(NK细胞)活性、抗体生成细胞水平及腹腔巨噬细胞吞噬功能的变化。结果:ETM的0.6、1.2、3.6g/(kg·d)三剂量组均可增强小鼠的脾淋巴细胞增殖能力、NK细胞活性及巨噬细胞吞噬指数水平;1.2、3.6g/(kg·d)剂量组可提高小鼠抗体生成细胞水平和巨噬细胞吞噬率。结论:蒲公英提取物具有增强小鼠免疫功能的作用。     

丝瓜水煎剂对小鼠免疫功能的影响

目的:探讨丝瓜水煎剂对小鼠免疫功能的影响。方法:375只小鼠随机平均分为空白对照组、丝瓜水煎剂低浓度组(2.5 g·kg-1)、丝瓜水煎剂中浓度组(5.0 g·kg-1)和丝瓜水煎剂高浓度组(10 g·kg-1)、泼尼松组(0.025 g·kg-1)。采用耳肿胀试验、碳廓清试验、脾淋巴细胞转化测定试验、淋巴细胞a-醋酸萘酯酶染色法以及小鼠血清中Ig G和Ig M含量检测试验,探讨丝瓜水煎剂对小鼠免疫功能的影响。结果:丝瓜水煎剂(10 g·kg-1)能明显减弱小鼠耳朵迟发型超敏反应(P0.01);抑制小鼠的单核巨噬细胞活性(P0.01);使小鼠淋巴细胞转化率明显降低(P0.01);减少小鼠血液中T淋巴细胞数(P0.01);明显降低小鼠血清中Ig G、Ig M含量(P0.01)。结论:丝瓜水煎剂高浓度组(10 g·kg-1)具有明显抑制小鼠免疫功能的作用。     

马齿苋合剂水提取液对感染单纯疱疹病毒Ⅱ型小鼠免疫功能的影响

目的：探讨马齿苋合剂水提取液对病毒感染性疾病的治疗作用及免疫调节的可能机制。方法：应用单纯疱疹病毒Ⅱ型（HSV-2）感染小鼠阴道的模型，灌胃给马齿苋合剂水提取液，并设对照。结果：马齿苋提取液能提高模型小鼠淋巴细胞转化率、脾脏淋巴细胞白细胞介素-2的分泌。经统计学处理，给药组、对照组差异有显著性（P〈0．05）。结论：马齿苋合剂水提液能增强腹腔巨噬细胞的吞噬功能，增加免疫器官重量。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.