635/808 nm双波长半导体激光与632.8 nm氦氖激光照射对术后切口愈合疗效的比较

目的 观察635/808 nm双波长半导体激光与氦氖（He-Ne）激光低强度照射对手术切口愈合疗效的比较.方法 选择非恶性肿瘤手术患者168例,随机分为2组,试验组83例,手术切口予以半导体激光治疗仪低强度照射;阳性对照组85例,手术切口予以He-Ne激光治疗仪照射,以伤口红、肿、热、痛、渗出、裂开等临床表现和不良事件以及切口长度差距为指标观察2组手术切口愈合情况.结果 试验组和对照组切口愈合情况差异无统计学意义（P＞0.05）.结论 双波长半导体激光照射对手术切口愈合的疗效与He-Ne激光治疗效果相当.     

音频调制半导体脉冲激光器的设计及对大鼠照射剂量的研究

目的:为了克服连续激光和固定脉冲激光长时间照射易使机体产生适应性而影响治疗效果的缺点,本文设计了一款基于音频调制的半导体脉冲激光器(波长650nm),并探索其改善大鼠血液流变性的照射剂量。方法:用多模式音频调制脉冲信号治疗仪作激励源,使激光器输出等强度、脉宽可变的脉冲激光,激光器的开关控制电路可调节激光的输出功率;对三组Wistar大鼠的耳尖穴每天分别用激光照射30min、45min、60min,10天后取血检测大鼠的全血粘度和血浆粘度。结果:激光器在脉冲输出状态,功率为50mW;无脉冲输出状态,连续光功率为15mW时,照射45min的大鼠全血粘度和血浆粘度值最低。结论:本文设计的激光系统在一定的照射剂量下对大鼠的血液流变性有较大的改善,提示可对早期高血压的降压研究提供参考。     

康肤霜对大鼠放射性皮肤损伤创面愈合的影响

背景:放疗中引起的放射性皮肤损伤,常使用重组人表皮生长因子和聚维酮碘治疗,但两者均有一定缺陷。而康肤霜能促进细胞分裂增殖,具有一定的抗菌消炎作用,可能对放射性皮肤损伤有很好的疗效。目的:观察康肤霜对大鼠放射性皮肤损伤创面愈合的影响。方法:高能X射线照射Wistar大鼠臀部皮肤建立深Ⅱ度皮肤烧伤创面模型。照射后12d创面出现后,将损伤动物随机分为康肤霜组,重组人表皮生长因子组和聚维酮碘组。各组每日将药物均匀涂抹在创面,直至创面取材或创面愈合。观察创面愈合率及愈合时间。各组动物分别于创面治疗后7,14,21d取创面组织,观察病理组织学变化、创面组织增殖细胞核抗原的表达,以标记指数法测定增殖细胞核抗原阳性细胞数,评估组织创面的修复情况。结果与结论:康肤霜组与重组人表皮生长因子组创面愈合时间、愈合率均优于聚维酮碘组(P0.01),但两组间无显著差异(P0.05)且两组创面组织标本中增殖细胞核抗原表达量也明显高于聚维酮碘组(P0.01),但其两组间比较差异无显著性意义(P0.05)。说明康肤霜能减轻创面早期的炎症反应,促进创面的再上皮化和表皮各层的分化,加速大鼠放射性皮肤损伤创面的愈合。     

脉冲式Ho：YAG激光消融效应的实验研究

为研究功率、照射时间、脉冲能量、脉冲频率等激光参数对Ｈｏ：ＹＡＧ激光肖融效应的影响,我们以５只犬食管作为实验对象,以不同功率、不同时间照射食管粘膜;对相同功率,又分为高脉冲能量低脉冲频率（Ｈ）组和低脉冲能量高脉冲频率（Ｌ）组进行配对研究,观察不同激光参数消融作用的差异。结果显示：脉冲式Ｈｏ：ＹＡＧ激光 融作用与功率和照射时间成正相关;Ｈ组消融作用大于Ｌ组;高功率的Ｈｏ：ＹＡＧ激光,主要表现出消融作     

7—7 氩激光照射及其剂量对大鼠实验性伤口愈合影响的研究

本文介绍用低功率密度、波长为514.5nm的氩离子激光,以17种不同的剂量分两批在不同季节对192只大鼠,384个皮肤伤口照射的实验,观察到伤口的平均愈合时间以及愈合速率跟所照射激光的剂量,照射天数等有关,并观察到氩激光照射有抑制作用。初步认为低功率密度氩离子激光(514.5nm)对皮肤伤口的愈合是可以产生促进作用的。     

基因重组人血小板源性生长因子(rhPDGF)药效学研究

目的:观察基因重组人血小板源性生长因子(recombinant human platelet-derived growth factor, rhPDGF)对皮肤创口愈合作用的影响。方法:以链脲霉素(streptozozin,STZ)所致糖尿病小鼠(STZ小鼠)以及非肥胖性糖尿病(non-obese diabetes, Nod)小鼠(Nod小鼠)为模型,制作全厚度皮肤切口,用rhPDGF连续治疗5天,观察rhPDGF对皮肤创口的愈合作用。结果:rhPDGF能明显促进糖尿病小鼠皮肤创口的愈合,10 μg/cm2 即有明显促进糖尿病小鼠皮肤创口愈合的作用。结论:rhPDGF具有促进STZ糖尿病小鼠和Nod小鼠皮肤创口愈合的作用。     

低强度激光联合照射口咽部和犊鼻穴对实验性膝骨关节炎兔氧自由基代谢的影响

背景:目前激光照射治疗膝骨关节炎以局部照射为主、输出功率和信号较为单一,多次照射易产生生物适应而降低疗效。目的:探讨携带有针灸补泻信息的低能量激光联合多部位照射对实验性膝骨关节炎兔血清和滑膜组织中氧自由基代谢的影响。方法:向兔膝关节腔内注入木瓜蛋白酶制备兔膝骨关节炎模型。穴位照射组和联合照射组分别以低能量激光单独照射犊鼻穴或联合照射口咽部及犊鼻穴,10min/d,5d为1个疗程,连续3个疗程。参照试剂盒方法测定血清一氧化氮、丙二醛水平和超氧化物歧化酶活性及膝关节滑膜组织丙二醛水平和超氧化物歧化酶活性;苏木精-伊红染色观察膝关节软骨组织形态学变化。结果与结论:兔膝骨关节炎后,软骨出现明显的病理损伤,血清一氧化氮、丙二醛水平均显著升高(P0.01或P0.05),超氧化物歧化酶活性显著降低(P0.05)。经激光穴位照射和激光联合照射后病理损伤有所减轻,血清一氧化氮、丙二醛水平均下降,超氧化物歧化酶活性升高,以联合照射组效果显著(P0.05)。兔膝关节滑膜组织中丙二醛水平和超氧化物歧化酶活性变化与血清中相一致。说明低能量激光联合照射口咽部和犊鼻穴能够纠正膝骨关节炎兔氧自由基代谢紊乱,减轻关节软骨细胞结构损伤,其效果优于激光单独照射犊鼻穴。     

点阵CO2激光作用皮肤光老化模型后表皮干细胞的分布特征

背景：临床应用点阵CO2激光治疗面部光老化已取得显著疗效,但点阵CO2激光作用于皮肤光老化模型创面修复过程中表皮干细胞的变化规律尚无研究。 目的：观察点阵CO2激光作用小鼠皮肤光老化模型后创面愈合过程中表皮干细胞数量及其在表皮中的分布变化规律。 方法：将10只昆明小鼠以UVB紫外线照射,制备成皮肤光老化模型,行点阵CO2激光(Deep FX)干预,观察激光创面愈合情况,并且于干预前、干预后第1,3,7,15天取材,行免疫组织化学染色观察角蛋白19和基因物质P63阳性表达。 结果与结论：干预后第15天,点阵CO2激光创面基本愈合。激光干预后第3天角蛋白19和基因物质P63阳性细胞率高于干预前及干预第1天,并出现阳性细胞分布范围扩大。第7天阳性细胞率明显高于第1,3天,为最高值,排列结构紊乱,分布到表皮各层。第15天阳性细胞率下降,仅集中于表皮的基底层和毛囊隆突,阳性表达与干预前及干预后第1天无明显差别。结果可见表皮干细胞参与了点阵CO2激光干预小鼠光老化皮肤的修复过程,可能在该过程中发挥重要作用。     

氩激光及其剂量对大鼠实验性伤口愈合作用的影响研究

本研究用低功率密度的波长为514.5nm的氩离子激光以12种不同的剂量照射大鼠皮肤伤口,观察到伤口的平均愈合时间以及愈合率跟所照射激光的剂量有关,还与照射天数有关。本文将本实验结果与国外文献进行了比较,并讨论了本结果的临床意义。     

Nd:YAG激光照射大鼠阴囊睾丸对精子发生的影响

以连续输出的Nd:YAG激光(输出功率7～10W,波长1.06μ)对大鼠阴囊睾丸进行一次性照射,维持局部温度43±0.5℃,持续15分钟。用组织学和组织化学方法观察了照射后1至90天曲细精管生精上皮和间质细胞的变化,证明了Nd:YAG激光对精子发生的抑制作用及其可逆性,探讨了Nd:YAG激光迅速产生抗生精效应的机制,以及对间质细胞功能的暂时影响等问题;提出了适当的照射条件。     

Low-Level Laser Therapy Facilitates Superficial Wound Healing in Humans: A Triple-Blind, Sham-Controlled Study

OBJECTIVE: Low-level laser therapy (LLLT) has been promoted for its beneficial effects on tissue healing and pain relief. However, according to the results of in vivo studies, the effectiveness of this modality varies. Our purpose was to assess the putative effects of LLLT on healing using an experimental wound model. DESIGN AND SETTING: We used a randomized, triple-blind, placebo-controlled design with 2 within-subjects factors (wound and time) and 1 between-subjects factor (group). Data were collected in the laboratory setting. SUBJECTS: Twenty-two healthy subjects (age = 21 +/- 1 years, height = 175.6 +/- 9.8 cm, mass = 76.2 +/- 14.2 kg). MEASUREMENTS: Two standardized 1.27-cm(2) abrasions were induced on the anterior forearm. After wound cleaning, standardized digital photos were recorded. Each subject then received LLLT (8 J/cm(2); treatment time = 2 minutes, 5 seconds; pulse rate = 700 Hz) to 1 of the 2 randomly chosen wounds from either a laser or a sham 46-diode cluster head. Subjects reported back to the laboratory on days 2 to 10 to be photographed and receive LLLT and on day 20 to be photographed. Data were analyzed for wound contraction (area), color changes (chromatic red), and luminance. RESULTS: A group x wound x time interaction was detected for area measurements. At days 6, 8, and 10, follow-up testing revealed that the laser group had smaller wounds than the sham group for both the treated and the untreated wounds (P < .05). No group x wound x time differences were detected for chromatic red or luminance. CONCLUSIONS: The LLLT resulted in enhanced healing as measured by wound contraction. The untreated wounds in subjects treated with LLLT contracted more than the wounds in the sham group, so LLLT may produce an indirect healing effect on surrounding tissues. These data indicate that LLLT is an effective modality to facilitate wound contraction of partial-thickness wounds.     

毛发生长周期对小鼠皮肤创伤愈合的影响

目的　探讨处于不同毛发生长周期的C57BL/6小鼠皮肤创伤愈合速度。 方法　制备小鼠皮肤创伤模型,计算术后0、3、7 d创面愈合率,愈合率=（原始创面面积－未愈合的创面面积）/原始创面面积×100%,比较毛发静止期（Hair telogen stages）小鼠和毛发生长期（Hair anagen stages）小鼠伤口愈合速度。采用HE染色比较伤口愈合的组织形态结构差异,利用BrdU检测伤口周围细胞增殖。 结果　毛发生长期的小鼠皮肤伤口愈合率显著高于毛发静止期的小鼠伤口愈合率。HE染色显示毛发生长期小鼠伤口周围表皮细胞层较多,且表皮细胞向伤口迁移增强;BrdU检测显示毛发生长期小鼠皮肤伤口周围表皮BrdU+ 细胞数多于毛发静止期小鼠。 结论　毛发生长期的小鼠皮肤创伤愈合率高于毛发静止期小鼠,这一结果为进一步探讨毛囊在创伤愈合过程中的作用提供研究基础,也为选择皮肤创伤愈合动物模型提供指导。     

The effect of Mindfulness-Based Stress Reduction on wound healing: a preliminary study

Psychological factors have been shown to influence the process of wound healing. This study examined the effect of Mindfulness-Based Stress Reduction (MBSR) on the speed of wound healing. The local production of pro-inflammatory cytokines and growth factors was studied as potential underlying mechanism. Forty-nine adults were randomly allocated to a waiting-list control group (n = 26) or an 8-week MBSR group (n = 23). Pre- and post-intervention/waiting period assessment for both groups consisted of questionnaires. Standardized skin wounds were induced on the forearm using a suction blister method. Primary outcomes were skin permeability and reduction in wound size monitored once a day at day 3, 4, 5, 6, 7, and 10 after injury. Secondary outcomes were cytokines and growth factors and were measured in wound exudates obtained at 3, 6, and 22 h after wounding. Although there was no overall condition effect on skin permeability or wound size, post hoc analyses indicated that larger increases in mindfulness were related to greater reductions in skin permeability 3 and 4 days after wound induction. In addition, MBSR was associated with lower levels of interleukin (IL)-8 and placental growth factor in the wound fluid 22 h after wound induction. These outcomes suggest that increasing mindfulness by MBSR might have beneficial effects on early stages of wound healing. Trial Registration NTR3652, http://www.trialregister.nl     

Regulation of K(+) channels may enhance wound healing in the skin

In the process of promoting wound healing, epidermal growth factor (EGF) activates protein kinase C, protein tyrosine kinase and ERK MAPK (mitogen-activated protein kinase). The activation of these mediators in signal pathways can regulate the operation of K(+) channels. In addition, the K(+) channel is involved with cell migration and proliferation, both of which are requisite for wound healing. Recent studies, although not conducted on skin wounds, have found that the K(+) channel is associated with wound healing and that wound healing can be promoted by regulating the K(+) channels. Therefore, the authors hypothesize that healing of skin wounds could be promoted by regulating K(+) channel distribution in skin keratinocytes or fibroblasts. We plan to conduct a study of the promotion of skin wound healing using K(+) channel regulators.     

烫伤创面愈合后的瘢痕形成与神经支配

背景：既往研究表明神经因素在创面愈合中具有重要调控作用,但有关神经调节与创面愈合后瘢痕形成之间的关系鲜有报道。 目的：观察烧伤创面愈合过程中神经支配与创面愈合质量之间的关系。 方法：将30只大鼠右侧T9~L1阶段脊神经根切断,制作失神经支配皮肤模型;然后在大鼠背部右侧失神经支配皮肤区域制作直径4 cm的深Ⅱ度烫伤创面,设为模型组,左侧对称正常皮肤制作同样的创面,设为对照组,伤后连续观察创面变化,于7,14,21 d采用免疫组织化学法观察Ⅰ和Ⅲ胶原分泌情况,并计算Ⅰ/Ⅲ型胶原比例的变化,探讨创面愈合速度及愈合质量。 结果与结论：模型组Ⅰ,Ⅲ型胶原分泌和Ⅰ/Ⅲ型胶原比值于伤后各时间点均显著低于对照组(P < 0.05)。模型组Ⅰ型胶原分泌与伤后7,14,21 d逐渐增加(P < 0.05),Ⅲ型胶原于伤后21 d时分泌明显增高(P < 0.05),Ⅰ/Ⅲ型胶原比值与伤后21 d时明显降低(P < 0.05)。结果证实,创面早期神经支配可以促进创面愈合,创面重塑期减轻神经支配可能会改善创面重塑质量。     

Meek微型皮片移植修复112例大面积深度烧伤创面的临床观察

目的观察Meek微型皮片移植技术修复大面积深度烧伤创面的疗效,探讨其临床使用价值及应用前景。方法采用Meek微型皮片移植治疗112例大面积深度烧伤作为治疗组,传统小邮票皮片移植方法治疗106例大面积深度烧伤患者作为对照组,观察对比两组皮片的成活率、创面的愈合时间、手术时间、愈合后的瘢痕及功能恢复情况等。结果治疗组皮片成活率≥96%,对照组皮片成活率≥85%。治疗组各项观察指标同对照组相比较有显著性差异（P〈0.01）。结论 Meek植皮方法可节省皮源、缩短手术时间、加快创面愈合、缩短病程和减轻愈合后的瘢痕。该手术方法操作简单,是大面积深度烧伤治疗中较理想的方法之一。     

Effect of Low-Power Laser (LPL) and Light-Emitting Diode (LED) on Inflammatory Response in Burn Wound Healing

The aim of the study was to investigate the biochemical and molecular changes in the process of epidermal healing of burn injuries after therapeutic treatment with low-power laser (LPL) and light-emitting diode (LED). Rats were divided into six groups: skin without injury (Sham), burn wounds (BWs), BW?+?660-nm LPL, BW?+?904-nm LPL, BW?+?632-nm LED, and BW?+?850-nm LED. The burn wound model was performed using a 100 °C copper plate, with 10 s of contact in the skin. The irradiations started 24 h after the lesion and were performed daily for 7 days. The burn wound groups showed an increase in the superoxide production, dichlorofluorescein, nitrites, and high protein oxidative damage. The activities of glutathione peroxidase and catalase were also increased, and a significant reduction in glutathione levels was observed compared to the control group. However, treatments with 660-nm LPL and 850-nm LED promoted protection against to oxidative stress, and similar results were also observed in the IL-6 and pERK1/2 expression. Taken together, these results suggest that LPL 660 nm and LED 850 nm appear reduced in the inflammatory response and oxidative stress parameters, thus decreasing dermal necrosis and increasing granulation tissue formation, in fact accelerating the repair of burn wounds.     

生物与合成敷料封闭负压引流促进植皮创面愈合的对比

背景：骨科创面植皮时采用封闭负压引流可以封闭创面,减少渗出,促进软组织肉芽增生及植皮修复缺损。 目的：对比两种不同材料的创面敷料封闭负压引流在创面植皮中的临床效果。 方法：选择2010年9月至2012年3月收治的80例因创伤需植皮患者,在植皮后按自愿原则进入生物材料(基于丝瓜的植物纤维为敷料主体)及合成材料(聚乙烯乙醇化海藻盐泡沫)创面敷料封闭负压引流治疗。治疗1周后拆除封闭负压引流装置及创面敷料,记录两组的植皮覆盖率及创面愈合时间。 结果与结论：两组患者均有效达到创面植皮覆盖效果,无感染及不愈合情况出现。采用生物材料创面敷料治疗组植皮覆盖率及创面愈合时间均明显优于采用合成材料创面敷料治疗组(P < 0.05)。提示采用生物材料创面敷料封闭负压引流可以有效促进引流,改善循环,抑制细菌增生及促进修复过程。中国组织工程研究杂志出版内容重点：生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程全文链接：     

同种异体皮在皮肤软组织严重毁损性创面修复中的应用

目的探讨同种异体皮覆盖急性毁损性创面,提高患者救治成功率及促进创面愈合的可行性。方法 回顾性分析56例急诊住院治疗皮肤软组织严重毁损性患者的临床病例,按治疗方法分组:急诊清创后同种异体皮覆盖创面,待二期修复创面的28例为治疗组;清创后一期皮瓣、植皮封闭创面的28例设为对照组,观察两组患者的救治成功率和创面封闭时间。结果 两组救治成功率分别为96.43%和78.57%,差异具有统计学意义(P0.05);实验组创面治愈时间(16.17±1.87)d与实验组(21.71±3.95)d,差异具有统计学意义(P0.01)。结论 皮肤软组织严重毁损性损伤急诊手术应以稳定患者全身情况为重点,创面使用同种异体皮覆盖待二期修复,可降低急诊手术的风险性,提高患者救治成功率,缩短治疗周期。