C反应蛋白和白细胞介素-6与胎膜早破绒毛膜羊膜炎的关系

目的探讨胎膜早破孕妇血清及羊水中白细胞介素-6（IL-6）和血清中C反应蛋白（CRP）含量与绒毛膜羊膜炎的关系。方法胎膜早破孕妇40例（胎膜早破组）及正常孕妇40例（对照组）各取羊水及肘静脉血5ml,经离心处理后取上清液及血清,检测其血清IL-6和CRP含量,以及羊水中IL-6含量,同时2组均取胎膜行病理检查。结果胎膜早破孕妇血清IL-6和母血CRP含量水平均较对照组高,尤其是以羊水中IL6水平升高更为敏感（P〈0.05）。绒毛膜羊膜炎患者18例,其血清、羊水中IL-6和血清CRP含量水平均明显高于非绒毛膜羊膜炎患者（P〈0.05或P〈0.01）。结论胎膜早破孕妇血清、羊水中IL-6和血CRP水平对诊断绒毛膜羊膜炎有临床应用价值。     

细胞因子IL-1、IL-6、IL-8与早产的关系

目的 探讨早产孕妇血清白细胞介素-1、白细胞介素-6、白细胞介素-8与早产的关系.方法 用酶联免疫吸附ELISA法检测17例早产孕妇血清IL-1、IL-6、IL-8水平,并与48例足月妊娠分娩孕妇比较.所有孕妇均在产后取胎盘胎膜4×4cm送病理检查,排除合并绒毛膜羊膜炎.结果 早产孕妇血IL-1、IL-6、IL-8水平显著高于对照组(P＜0.05).结论 IL-1、IL-6、IL-8可能在早产的发生中起重要作用.     

血清降钙素原及白细胞介素-6与胎膜早破患者并发绒毛膜羊膜炎的相关性研究

目的探讨降钙素原及白细胞介素-6与胎膜早破的关系以及对绒毛膜羊膜炎的诊断意义。方法选取足月胎膜早破患者73例,未足月胎膜早破患者54例,足月及未足月健康孕妇各50例,检测血清PCT及IL-6。所有孕妇分娩后对胎盘、胎膜进行病理检查。结果足月PROM组与PPROM组的PCT、IL-6阳性率均高于对照组,差异有统计学意义(P<0.05)。胎膜早破患者中有组织学绒毛膜羊膜炎与无组织学绒毛膜羊膜炎血清PCT及IL-6比较,差异有统计学意义(P<0.05)。有组织学绒毛膜羊膜炎患者血清PCT及IL-6均阳性的比例高于无组织学绒毛膜羊膜炎组,其差异有统计学意义(P<0.05)。PCT对诊断组织学绒毛膜羊膜炎的敏感性、特异性、阳性预测值、阴性预测值分别为72.88%、70.59%、68.25%、75%,IL-6对诊断组织学绒毛膜羊膜炎的敏感性、特异性、阳性预测值、阴性预测值分别为83.05%、67.65%、69.01%、82.14%。联合检测PCT和IL-6对诊断组织学绒毛膜羊膜炎的敏感性、特异性、阳性预测值、阴性预测值分别为67.80%、82.35%、76.92%、78.67%。结论 PCT及IL-6都是胎膜早破并发绒毛膜羊膜炎较好的诊断指标,联合二者检测比单独检测无明显优势。     

基质金属蛋白酶9与白细胞介素6和白细胞介素8水平与胎膜早破患者绒毛膜羊膜炎的关系研究

目的 探讨基质金属蛋白酶（MMP-9）、白细胞介素-5（IL-5）和IL-8对早产胎膜早破孕妇患绒毛膜羊膜炎的关系.方法 84例早产胎膜早破孕妇作为研究组,根据有无绒毛膜羊膜炎将其分为羊膜炎亚组（52例）与无羊膜炎亚组（32例）,以48例正常孕妇作为对照组.采用酶联免疫吸附法（ELISA）检查各组孕妇的血清MMP-9、IL-5和IL-8,同步取胎盘胎膜组织进行病理组织学检查.结果 研究组孕妇MMP-9、IL-6和IL-8均高于对照组,羊膜炎亚组血清MMP-9、IL-6和IL-8 [（248.36±121.34）μg/L、（436.37 ±167.78）μg/L、（612.54±216.3）μg/L]均明显高于无羊膜炎亚组[（116.56 ±67.87）μg/L、（269.39±169.67）μg/L、（349.35 ±168.34）μg/L],差异有统计学意义（P＜0.05）;绒毛膜羊膜炎患者血MMP-9与IL-5和IL-8含量呈正相关（P＜0.05）.结论 孕妇血中MMP-9、IL-5和IL-8的联合测定,可作为监测绒毛膜羊膜炎的重要指标.     

白细胞介素-6及C反应蛋白检测对预测胎膜早破合并绒毛膜羊膜炎的意义

目的 探讨检测母血白细胞介素-6（IL-6）及C反应蛋白（CRP）对因胎膜早破（PROM）而导致的绒毛膜羊膜炎（CMA）早期预测的意义.方法 采集胎膜早破孕妇的血清测定IL-6及CRP含量,并对产妇的胎盘及胎膜采样进行病理检查.结果 （1）PROM合并CMA者,母血IL-6、CRP含量明显升高（P＜0.01）.（2）随着破膜时间的延长,母血IL-6及CRP的水平明显升高（P＜0.01）.（3）母血IL-6≥11 ng/L、CRP≥10 mg/L为预测诊断PROM合并早期CMA的阳性率高.结论 母血IL-6及CRP水平的监测对PROM合并CMA有一定的早期诊断意义.     

血清降钙素原与胎膜早破的关系研究

目的检测未足月胎膜早破(PPROM)与足月胎膜早破(足月PROM)孕妇血清降钙素原(PCT)的浓度,探讨其与胎膜早破的关系及对PPROM患者绒毛膜羊膜炎的预测价值。方法选取PPROM患者55例,相同孕周健康孕妇40例;足月PROM患者40例,相同孕周健康孕妇40例,进行血清PCT检测,孕足月PROM及PPROM患者分娩后胎盘胎膜病理检查。结果孕足月PROM及PPROM组孕妇血清中PCT阳性率均高于相应对照组(P<0.05);但足月PROM及PPROM组孕妇血清中PCT比较,差异无统计学意义(P>0.05)。且PPROM组中存在组织学绒毛膜羊膜炎者与无绒毛膜羊膜炎者血清PCT差异无统计学意义(P>0.05);PCT诊断PPROM患者组织学绒毛膜羊膜炎的敏感性、特异性分别为60.00%、35.00%(PCT>0.5μg/L);37.00%、60.00%(PCT>2μg/L)。结论PCT在PPROM及孕足月PROM孕妇血清中浓度升高,但其对PPROM患者绒毛膜羊膜炎的预测价值尚待进一步研究。     

白细胞介素-6及C反应蛋白检测对预测胎膜早破合并绒毛膜羊膜炎的意义

Objective To investigate significance of interleukin-6 and C-reactive protein as a predictor of chorioamnionitis in patients with premature rupture of the membranes. Methods Maternal serum was collected for detection of IL-6 and CRP, and placenta and membranes sample for pathology examination. Results ( 1 ) The level of maternal serum CRP and IL-6 raised signficantly in patients with premature rupture of membranes complicated by chorioamnionitis(P ＜0.01). (2)The levels of maternal serum CRP and IL-6 also raised signficantly with the time prolongation of premature rupture of themembranes( P ＜ 0. 0l ). ( 3 ) The positive rate of the predict diagnosis of PROM with early CMA was promoted by the standard of CRP≥10 mg/L and IL-6≥11 ng/L. Conclusion The maternal serum CRP and IL-6 determination could be used for the prediction of early CMA.     

胎膜早破与绒毛膜羊膜炎关系的临床分析

目的分析胎膜早破与绒毛膜羊膜炎的关系。方法选取我院收治的胎膜早破患者210例,观察其中绒毛膜羊膜炎发生情况,分析绒毛膜羊膜炎发生的危险因素。结果未足月胎膜早破患者绒毛膜羊膜炎发生率(32.50%)高于足月胎膜早破患者(17.06%),差异有统计学意义(P <0.05)。胎膜早破患有阴道炎的患者绒毛膜羊膜炎发生率(28.13%)高于未患有阴道炎的患者(16.44%),差异有统计学意义(P <0.05)。胎膜早破患有妊娠期糖尿病患者的绒毛膜羊膜炎发生率(18.18%)与未患有妊娠期糖尿病患者(20.21%)比较,差异无统计学意义(P> 0.05)。结论伴有阴道炎或者未足月妊娠的胎膜早破患者有着较高的绒毛膜羊膜炎的发生概率,临床医师需要特别注意。     

胎膜早破绒毛膜羊膜炎与新生儿预后的关系

目的:探讨胎膜早破及绒毛膜羊膜炎与新生儿预后的关系.方法:对我院80例胎膜早破病例进行回顾性分析.结果:胎膜早破并发绒毛膜羊膜炎率较高,早产胎膜早破组达53.8%.绒毛膜羊膜炎组新生儿窒息、新生儿肺炎、病理性黄疸中的发病率高于非绒毛膜羊膜炎组(P＜0.05).病理分类不同,但孕妇外周血的白细胞表达无显著性差异.结论:胎膜早破与绒毛膜羊膜炎及感染关系密切,胎膜早破绒毛膜羊膜炎与新生儿预后有密切关系,应重视亚临床型的绒毛膜羊膜炎.     

IL-6、IL-8 及 MMP-9 在胎膜早破者血清、羊水中的含量及意义简

目的 探讨孕妇血清、羊水中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、基质金属蛋白酶-9（MMP-9）对胎膜早破、宫内感染的临床意义.方法 某院产科住院分娩的胎膜早破孕妇100例为研究组,对照组为同期住院无妊娠并发症剖宫产的孕妇100例.采用酶联免疫吸附法检测IL-6、IL-8及MMP-9在孕妇血清、羊水中的含量.同时产后取胎膜送病理检查.结果 研究组孕妇血清、羊水中IL-6、IL-8及MMP-9的含量均高于对照组,差异有统计学意义（P〈0.05）;宫内感染者孕妇血清、羊水中IL-6、IL-8及MMP-9水平高于非宫内感染者,差异有统计学意义（P〈0.05）.随着破膜时间延长,孕妇血清、羊水中IL-6、IL-8及MMP-9的含量增高.结论 IL-6、IL-8及MMP-9联合检测可用于胎膜早破及宫内感染的早期诊断,并指导临床用药,对确定终止妊娠的最佳时机也具有一定的临床意义.     

Secretion of macrophage migration inhibitory factor differs from interleukin-6 in hydrogen peroxide- and LPS-stimulated human fibroblasts

To investigate the mechanism for secretion of macrophage migration inhibitory factor (MIF) in cultured human fibroblasts, we compared it with the secretion of interleukin-6 (IL-6) after stimulation with lipopolysaccharides (LPS) and H2O2. MIF content of the medium of 2.0 x 10(6) cells/20 ml after 20 h culture of nonstimulated fibroblasts was 0.30 +/- 0.06 ng/ml, whereas LPS-stimulation (10 microg/ml) only led to a 1.5-fold increase as compared with the nonstimulated cells. In contrast, a significant increase of IL-6 was induced by LPS-stimulation (6048 +/- 488 pg/ml in LPS-stimulated cells vs. 58 +/- 36 pg/ml in control cells). On the other hand, higher concentrations of H2O2 (0.6-1.2 mM) caused an increase of MIF secretion into the culture medium irrespective of LPS-stimulation; with 1.2 mM H2O2-stimulation for 20 h, it was increased to 40-fold as compared with the nonstimulated cells. However, lower concentrations (0.1-0.4 mM) did not cause this. Interestingly, H2O2-stimulation not only failed to increase IL-6 production from fibroblasts, but also repressed induction of IL-6 by LPS-stimulation in a dose-dependent manner. Genistein, a tyrosine kinase inhibitor, and H-7, a protein kinase C inhibitor, also inhibited IL-6 secretion but not MIF secretion in both LPS- and H2O2-stimulated fibroblasts. From analysis of trypan blue exclusion, formazan formation, morphological changes, and intracellular MIF content by Western blotting, we found that MIF secretion by H2O2 seemed to be mainly due to cell death and subsequent leakage of intracellular MIF. Taken together, these results suggest that MIF secretion differs from IL-6 via LPS-mediated signaling pathways.     

巨噬细胞移动抑制因子在肝门部胆管癌中的表达及其临床意义

目的:探讨巨噬细胞移动抑制因子(MIF)在肝门部胆管癌(HCCA)中的表达及其临床意义.方法:选取新疆医科大学第一附属医院2019年5月至2020年5月经手术治疗的30例肝门部胆管癌患者(HCCA组)和30例同期健康体检者(对照组)为研究对象.分析HCCA患者癌组织病理特征、糖原合成和储存情况及MIF表达情况;比较两组...     

Aza-derivatives of resveratrol are potent macrophage migration inhibitory factor inhibitors

Resveratrol (3, 4', 5-trihydroxy-trans-stilbene), a natural phytoalexin found in grapes and wine, has anti-proliferative activity on human-derived cancer cells. In our study, we used a conventional condensation reaction between aldehydes and amines to provide a number of aza-resveratrol (3, 4', 5-trihydroxy-trans- aza-stilbene) derivatives in an attempt to screen for compounds with resveratrol's action but with increased potency. Aza-resveratrol and its hydroxylated derivative (3, 4, 4', 5-tetrahydroxy-trans- aza-stilbene) showed a more enhanced anti-proliferative effect than resveratrol in an MCF-7 breast carcinoma cell line. To identify the cellular targets of the aza derivatives of resveratrol, we conjugated the latter aza-stilbene compound with epoxy-activated agarose and performed affinity purification. Macrophage migration inhibitory factor (MIF), a proinflammatory cytokine, was identified as a major target protein in MCF-7 cell lysates using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS). The aza-resveratrol and its hydroxylated derivative, but not resveratrol, were also found to be potent inhibitors of MIF tautomerase activity, which may be associated with their inhibitory effects on MIF bioactivity for cell growth.     

巨噬细胞移动抑制因子对2型糖尿病患者血管病变的影响

目的探讨2型糖尿病(T2DM)患者血清巨噬细胞移动抑制因子(MIF)水平变化及其对糖尿病血管病变的影响。方法用ELISA法检测T2DM合并血管病变患者(A组,19例)、T2DM无血管病变患者(B组,19例)和健康体检者(C组,16例)血清MIF和超敏C反应蛋白(hs-CRP)。同时检测血糖(BG)和糖化血红蛋白(HbA1c)。结果 A组和B组MIF、hs-CRP水平均高于C组(P<0.01)。A组hs-CRP与血糖呈正相关。B组MIF与hs-CRP、BG水平呈正相关,hs-CRP与BG、HbA1c水平呈正相关。结论糖尿病血管病变患者MIF与hs-CRP高表达是促进血管病变发生的重要因素。     

Effect of serum macrophage migration inhibitory factor on type-2 diabetes-related angiopathy

目的 探讨2型糖尿病(T2DM)患者血清巨噬细胞移动抑制因子(MIF)水平变化及其对糖尿病血管病变的影响.方法 用ELISA法检测T2DM合并血管病变患者(A组,19例)、T2DM无血管病变患者(B组,19例)和健康体检者(C组,16例)血清MIF和超敏C反应蛋白(hs-CRP).同时检测血糖(BG)和糖化血红蛋白(HbAlc).结果 A组和B组MIF、hs-CRP水平均高于C组(P＜0.01).A组hs-CRP与血糖呈正相关.B组MIF与hs-CRP、BG水平呈正相关,hs-CRP与BG、HbAlc水平呈正相关.结论 糖尿病血管病变患者MIF与hs-CRP高表达是促进血管病变发生的重要因素.     

巨噬细胞移动抑制因子（MIF）参与炎症性疾病糖皮质激素抵抗机制的研究进展

糖皮质激素（GC）具有强大的抗炎作用,是临床上使用最为广泛的免疫抑制剂,然而部分患者在使用激素治疗一段时间后会出现激素抵抗性。巨噬细胞移动抑制因子（MIF）是截至目前被认为唯一能负向调节GC抗炎作用的细胞因子,但MIF在糖皮质激素抵抗机制中扮演什么样的角色,尚未完全阐明。对MIF参与抵抗糖皮质激素抗炎作用的机制进行综述。