高效液相色谱法测定奈韦拉平片有关物质及其含量

目的采用高效液相色谱仪建立奈韦拉平片中有关物质及其含量的测定方法,为其质量控制提供参考。方法采用ODS-C18柱(250mm×4.6mm,5μm);流动相:乙腈-0.025mol/L磷酸二氢铵溶液(20∶80);流速:1.0ml/min;检测波长:220nm。结果线性范围为5～100μg/ml(r=1.000 0)检测限为0.9ng,样品在24h内稳定性、精密度良好,RSD=0.2%,平均回收率为100.36%。结论所建立的高效液相色谱法简便、快速、准确、专属性高,适用于奈韦拉平片中有关物质及其含量的测定。     

HPLC法测定奈韦拉平片的含量及有关物质

目的高效液相色谱法测定奈韦拉平片的含量及有关物质的研究。方法采用C18色谱柱 (5 μm ,4 .6mm× 2 5 0mm)。以ψ(乙腈 ∶水 ) =2 3∶77为流动相 ,流速 1.0mL/min ,紫外检测器于 2 14nm测定。结果HPLC法的线性范围为 0 .5～ 12 5 μg/mL ,r=0 .9999,最低检测限 2ng,样品溶液在 8h内稳定 ,日内精密度和日间精密度良好 (RSD <0 .5 % ) ,回收率为 99.6 %。结论采用HPLC法测定奈韦拉平片的含量及有关物质的方法简便 ,结果准确可靠     

等吸收双波长分光光度法同时测定芦荟中芦荟大黄素和芦荟甙

目的　建立紫外可见分光光度法同时测定芦荟大黄素和芦荟甙的方法。　方法　根据双波长原理 ,分别在测定波长 2 5 5 ,376 nm ,参比波长 35 5 ,4 75 nm处测定芦荟大黄素和芦荟甙含量。　结果　芦荟大黄素在 2 .0 0× 10 - 6 ～ 5 .0 0× 10 - 5mol/ L浓度范围内与光密度 (D)呈线性关系 ,r=0 .9995 ;芦荟甙在 2 .0 0× 10 - 6 ～ 6 .0 0× 10 - 5mol/ L浓度范围内与 D呈线性关系 ,r=0 .9998。　结论　等吸收双波长分光光度法操作快速、简便、准确 ,无需分离可同时测定芦荟样品中两组分含量。     

薄层扫描法测定蒙药查干—汤中异土木香内酯的含量

目的 :研究测定查干 -汤中异土木香内酯含量的方法。方法 :采用双波长扫描法 ,测定波长 6 0 0nm ,参比波长 42 0nm。结果 :在 0 . 5 4～ 3. 2 4μg范围内呈良好线性关系 ,回归方程为 :Y =0 .35 82 +5 .2 77× 10 -5X(X的单位 μg) ,r =0 .9997。回收率为 10 0 .7% (RSD =1.72 % ,n =5 )。结论 :方法简便、准确。     

吸附溶出伏安法测定甲氧苄氨嘧啶

本文介绍了用2.5次微分吸附溶出伏安测定甲氧苄氨嘧啶(TMP)的新方法.在选定的实验条件下,TMP的浓度在2.5×10~(?)～1.0×10~(-7)mol/L范围内与峰高成线性关系,最低检测下限达1.0×10~(-9)mol/L(富集180s).用于TMP片剂含量分析,其相对标准偏差为6.5%(n=11),其回收率为102.4%.     

HPLC法测定奈韦拉平片有关物质及含量

目的 建立HPLC法对奈韦拉平片剂有关物质及含量进行测定,为本品的检验质控提供参考。方法 采用岛津ODS-C18 柱(250mm?4.6mm,5μm);流动相：乙腈-0.025 moL.L-1磷酸二氢铵溶液(用氢氧化钠调ph=5.0)(20:80);流速：1.0 mL.min-1 ;检测波长：220nm。     

HPLC法测定复方诺氟沙星涂剂中诺氟沙星的含量

目的　测定复方诺氟沙星涂剂中诺氟沙星的含量。方法　采用高效液相色谱法 ,色谱柱为Nova -PakC1 83.9× 15 0mm ,流动相为 0 .0 2 5mol L磷酸溶液 (用三乙胺调节pH值至 3.0± 0 .1) -乙腈 (90∶10 ) ,流速 0 .8ml min ,检测波长 2 78nm ,柱温 2 0℃。结果　诺氟沙星在 5～ 5 0mg L范围内线性关系良好 ,r =0 .9999;日内、日间差RSD均不超过 3% (n =5 ) ;平均回收率为 10 3.0 5 %。结论　该法简便、灵敏、快速、准确 ,可用于复方诺氟沙星涂剂中诺氟沙星含量的测定     

紫外分光光度法测定盐酸奈必洛尔片的含量

目的:建立盐酸奈必洛尔片剂含量的紫外分光光度测定法。方法:采用紫外分光光度法,以0.1mol/mL盐酸为空白对照,280nm为测定波长,测定盐酸奈必洛尔片的含量。结果:盐酸奈必洛尔在20.32～60.96μg/mL范围内线性关系良好(r=0.999 9,n=5),平均加样回收率为100.9%,RSD为1.14%(n=6)。结论:该方法操作简便、精密度好、结果可靠,适用于盐酸奈必洛尔片含量的快速测定。     

阿昔洛韦擦剂的制备及检定

目的　根据临床需要 ,我院自制了阿昔洛韦擦剂 ,其制备简便易行 ,质量易于控制。方法　含量测定方法为紫外分光光度法。结果　测定波长为 2 51.5nm ,线性方程为C =15.2 994A - 3.5774× 10 -1,线性范围为 2 .154～ 8.30 7μg ml,回收率为 99.2 3% ,RSD =0 .0 58% (n =5)。结论　阿昔洛韦擦剂在临床使用过程中能保证安全有效     

奈福泮玻璃涂膜电极的研究与应用

目的　研究离子选择性电极法测定奈福泮的含量。方法　在对奈福泮敏感的玻璃涂膜电极法。结果　电极的Nernst响应范围为 5 0× 10 2 ～ 5 0× 10 -5mol/L ,斜率为 5 4 6mV/ pc,检测限为 1 9× 10 5mol/L。结论　应用本法测定奈福泮的含量 ,结果与紫外分光光度法相符。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.