肌动蛋白交联蛋白与血管内皮生长因子在肾细胞癌中的表达及其相关性

Objective To investigate the expression of Fascin and vascular endothelial growth factor (VEGF) in renal cell carcinoma (RCC) and the correlation with the biological behaviors. Methods The immunohistochemistry staining method was used to detect the expression of Fascin and VEGF in 92 cases of RCC and 20 cases of normal renal tissues as controls. Results The expression of Fascin in carcinoma tissue was significantly higher than that in normal tissues ( P ＜ 0. 05 ). Positive expression of Fascin and VEGF in renal cell carcinoma tissue was correlated with tumor grade ( P ＜ 0. 05 ) and clinical stage (P ＜0. 05 ) , but not with age, gender and different histological categories (P ＞ 0. 05 ). There was also a positive correlation between Fascin and VEGF (P ＜ 0. 05 ). Conclusion Fascin and VEGF are objective markers to estimate the behaviors of renal cell carcinoma.     

肌动蛋白交联蛋白与血管内皮生长因子在肾细胞癌中的表达及其相关性

目的 探讨肌动蛋白交联蛋白(Fascin)与血管内皮生长因子(VEGF)在肾细胞癌组织的表达及与其生物学行为的关系.方法 采用免疫组织化学SP法检测Fascin与VEGF在92例肾细胞癌组织及20例正常肾脏组织中的表达.结果 Fascin及VEGF在肾细胞癌组织中的表达阳性率明显高于正常肾组织(P＜0.05);肾细胞癌组织中两种蛋白的表达与肿瘤组织学分级和临床分期均呈正相关(P＜0.05);与年龄、性别及肿瘤病理类型无明显相关(P＞0.05).且Fascin和VEGF的表达之间呈正相关(P＜0.05).结论 Fascin与VEGF可作为反映肾细胞癌生物学行为的参考指标.

Abstract：

Objective To investigate the expression of Fascin and vascular endothelial growth factor (VEGF) in renal cell carcinoma (RCC) and the correlation with the biological behaviors. Methods The immunohistochemistry staining method was used to detect the expression of Fascin and VEGF in 92 cases of RCC and 20 cases of normal renal tissues as controls. Results The expression of Fascin in carcinoma tissue was significantly higher than that in normal tissues ( P ＜ 0. 05 ). Positive expression of Fascin and VEGF in renal cell carcinoma tissue was correlated with tumor grade ( P ＜ 0. 05 ) and clinical stage (P ＜0. 05 ) , but not with age, gender and different histological categories (P ＞ 0. 05 ). There was also a positive correlation between Fascin and VEGF (P ＜ 0. 05 ). Conclusion Fascin and VEGF are objective markers to estimate the behaviors of renal cell carcinoma.     

瘦素和瘦素受体在食管癌中的表达及其意义

Objective To investigate the role of leptin and leptin receptor in carcinogensis and development of esophageal carcinoma. Methods The expression of leptin and leptin receptor was detected in 52 cases of esophageal carcinoma tissues and 49 cases of normal esophageal tissues by immunohistochemistry. The correlation between their expression and clinicopathological parameters was also analysized. Results The expression rate of leptin and leptin receptor in esophageal carcinoma was 78. 8% (41/52) and 82.7% (43/52) respectively, and the rate in normal esophagus was 58.3% (28/49) and 59.2% (29/49) respectively. The expression rate of leptin and leptin receptor both had statistically significantly differences between esophageal carcinoma and normal esophagus tissues (P ＜ 0. 05). The expression rate of leptin was associated with position, tumor size, differentiation, lymphatic metastasis and TNM stage (P ＜ 0. 05 ). Conclusion Leptin and leptin receptor were dually expressed in esophageal carcinoma.They played important roles in the process of carcinogensis and development of esophageal carcinoma.     

瘦素和瘦素受体在食管癌中的表达及其意义

Objective To investigate the role of leptin and leptin receptor in carcinogensis and development of esophageal carcinoma. Methods The expression of leptin and leptin receptor was detected in 52 cases of esophageal carcinoma tissues and 49 cases of normal esophageal tissues by immunohistochemistry. The correlation between their expression and clinicopathological parameters was also analysized. Results The expression rate of leptin and leptin receptor in esophageal carcinoma was 78. 8% (41/52) and 82.7% (43/52) respectively, and the rate in normal esophagus was 58.3% (28/49) and 59.2% (29/49) respectively. The expression rate of leptin and leptin receptor both had statistically significantly differences between esophageal carcinoma and normal esophagus tissues (P ＜ 0. 05). The expression rate of leptin was associated with position, tumor size, differentiation, lymphatic metastasis and TNM stage (P ＜ 0. 05 ). Conclusion Leptin and leptin receptor were dually expressed in esophageal carcinoma.They played important roles in the process of carcinogensis and development of esophageal carcinoma.     

肾细胞癌组织中DLK1蛋白表达及与临床病理学特征的相关性研究

目的 探讨不同组织类型的肾细胞癌组织中DLK1蛋白表达与肾癌临床病理特征及转移的关系.方法 采用免疫组织化学方法,检测94例肾原发透明细胞癌、76例乳头状肾细胞癌、45例嫌色细胞癌、71例透明细胞癌远处转移灶、24例透明细胞癌淋巴结转移灶及18例正常肾组织标本中DLK1蛋白表达情况,分析其与临床病理特征及转移的相关性.结果 正常肾组织近端及远端肾小管中DLK1蛋白表达均为阳性,透明细胞癌、乳头状肾细胞癌及嫌色细胞癌组织低表达率分别为33.0%(31/94)、27.6%(21/76)及33.3%(15/45),与正常肾组织相比,差异有统计学意义(P＜0.05),3种类型肾癌组织之间DLK1蛋白表达差异无统计学意义(P＞0.05).DLK1蛋白表达水平与透明细胞癌患者性别(男60例,女34例)、年龄(≥55岁50例,＜55岁44例)、病理分期(Ⅰ期41例,Ⅱ期9例,Ⅲ期21例,Ⅳ期23例)及淋巴结转移状态(无转移76例,有转移18例)等无明显相关性(P＞0.05).透明细胞癌原发灶、淋巴结转移灶、远处转移灶肿瘤组织中DLK1蛋白表达差异无统计学意义(P＞0.05).结论 不同类型的肾细胞癌组织中DLK1蛋白表达降低.DLK1蛋白低表达与透明细胞癌的临床病理特征及肿瘤转移无关.

Abstract：

Objective To identify the expression of DLK1 protein in different types of renal cell carcinomas and its correlations with pathological characteristics and metastasis. Methods Immunohistochemistry analysis was performed to evaluate the expression of DLK1 protein in 94 cases of primary clear cell renal cell carcinoma, 76 cases of papillary renal cell carcinoma, 45 cases of chromophobe renal cell carcinoma, 71 cases of distal metastatic and 24 cases of lymph node metastatic clear cell renal cell carcinoma, as well as 18 cases of normal renal tissue. The correlations of DLK1 protein expression with pathological characteristics were analyzed. Results DLK1 protein was expressed in proximal and distal renal tubular epithelial cells in all the normal renal cases. In contrast, DLK1 protein expression was lower in different types of renal cell carcinoma. The low or negative expression of DLK1 protein in clear cell renal cell carcinoma, papillary renal cell carcinoma and chromophobe renal cell carcinoma was 33.0% (31/94), 27.6% (21/76) and 33.3% (15/45), respectively. Compared to normal renal tissue, DLK1 protein expression was significantly down-regulated in renal cell carcinomas (P＞0.05), whereas there was no significant difference on DLK1 protein expressions among the different types (P＞0.05) of renal cell carcinomas. DLK1 protein expression was not correlated with sex (60 male and 34 female cases), age (≥55, 50 cases and 55, 44 cases), grade (41 cases in grade I, 9 cases in grade II, 21 cases in grade III and 23 cases in grade Ⅳ respectively) and lymph node metastasis (76 cases with and 18 cases without lymph node metastasis) in clear cell renal cell carcinoma (P＞0.05). There was also no significant difference among primary, lymph node and distal metastatic lesions of clear cell carcinoma (P＞0.05). Conclusions DLK1 protein expression is commonly down-regulated in different types of renal cell carcinomas. Down-regulation of DLK1 protein expression is not associated with pathological characteristics and metastasis in clear cell renal cell carcinoma.     

结直肠癌组织中p27基因甲基化与其临床病理的关系

Objective To investigate the relationship between p27 gene methylation and pathology of colorectal carcinoma. Methods p27 gene methylation promotor region and p27 protein expression were detected respectively by methylation specificity polymerase chain reaction and immunohistochemical staining SP in 106 cases of colorectal carcinoma and each adjacent normal mucous membrane tissue and 22 cases of colorectal adenoma tissue. Results The positive expression rate of p27 gene methylation was statistically different in colorectal carcinoma tissue compared with normal mucous membrane and colorectal adenoma tissue (P＜0.05). Their positive expression rate were 59.4% (63/106), 18.2% (4/22) and 3.8%(4/106) respectively in colorectal carcinoma tissue,colorectal adenoma and normal mucous membrane tissue (P ＜ 0. 05). p27 gene methylation in poorly differentiated group was significantly higher than that in welldifferentiated group (48.0% vs. 24. 7%, P ＜0. 05), in Dukes-A + B stage group was significantly lower than that in Dukes C + D stage group(20. 0% vs. 41.2%, P ＜ 0. 05 ), and it was higher in lymph nodes metastases group than that in lymph nodes negative group(41.5% vs. 23. 1%, P ＜0. 05), that in positive serosa infiltration group was higher than negative serosa infiltration group(32. 5% vs. 24. 1%, P ＞ 0. 05 ).Conclusions Methylated p27 gene protein expression in colorectal carcinoma was significantly higher than normal mucous membrane and colorectal adenoma tissue. The methylation rate of p27 gene in colorectal carcinoma was significantly associated with tumor differentiation, invasive depth, Dukes stage, lymph node metastasis.     

结直肠癌组织中p27基因甲基化与其临床病理的关系

Objective To investigate the relationship between p27 gene methylation and pathology of colorectal carcinoma. Methods p27 gene methylation promotor region and p27 protein expression were detected respectively by methylation specificity polymerase chain reaction and immunohistochemical staining SP in 106 cases of colorectal carcinoma and each adjacent normal mucous membrane tissue and 22 cases of colorectal adenoma tissue. Results The positive expression rate of p27 gene methylation was statistically different in colorectal carcinoma tissue compared with normal mucous membrane and colorectal adenoma tissue (P＜0.05). Their positive expression rate were 59.4% (63/106), 18.2% (4/22) and 3.8%(4/106) respectively in colorectal carcinoma tissue,colorectal adenoma and normal mucous membrane tissue (P ＜ 0. 05). p27 gene methylation in poorly differentiated group was significantly higher than that in welldifferentiated group (48.0% vs. 24. 7%, P ＜0. 05), in Dukes-A + B stage group was significantly lower than that in Dukes C + D stage group(20. 0% vs. 41.2%, P ＜ 0. 05 ), and it was higher in lymph nodes metastases group than that in lymph nodes negative group(41.5% vs. 23. 1%, P ＜0. 05), that in positive serosa infiltration group was higher than negative serosa infiltration group(32. 5% vs. 24. 1%, P ＞ 0. 05 ).Conclusions Methylated p27 gene protein expression in colorectal carcinoma was significantly higher than normal mucous membrane and colorectal adenoma tissue. The methylation rate of p27 gene in colorectal carcinoma was significantly associated with tumor differentiation, invasive depth, Dukes stage, lymph node metastasis.     

Mir-145在肝癌细胞及肝癌组织中的表达及其意义

Objective To explore the role of miR-145 in human hepatocellular carcinoma ( HCC). We investigate the expression of miR-145 in human embryonic hepatocytes ( L02 cells),hepatocellular carcinoma cell lines (MHCC97 ,SMMC7721) ,HCC tissues and normal liver. Methods We detected the expressions of miR-145 in human embryonic hepatocytes, hepatocellular carcinoma cell lines ( MHCC97,SMMC7721) ,HCC tissues,cancer organizations and normal liver by real-time PCR. Result The expression of miR-145 in hepatocellular carcinoma cell lines ( MHCC97,SMMC7721) is significantly lower than the human embryonic hepatocytes ( 0. 312 ± 0. 025,0. 396 ± 0. 076,0. 954 ± 0. 037, P ＜ 0. 05), and compared with the cancer organizations and normal liver,the expression of miR-145 in HCC tissues is significantly decreased (0. 162 ±0.002,0. 972 ±0.054,0.956 ±0. 018,P＜0. 05). Conclusion These results indicate that the miR-145 probably involved in HCC pathogenesis and was the indicator of diagnosis and prognosis of the HCC.     

Mir-145在肝癌细胞及肝癌组织中的表达及其意义

Objective To explore the role of miR-145 in human hepatocellular carcinoma ( HCC). We investigate the expression of miR-145 in human embryonic hepatocytes ( L02 cells),hepatocellular carcinoma cell lines (MHCC97 ,SMMC7721) ,HCC tissues and normal liver. Methods We detected the expressions of miR-145 in human embryonic hepatocytes, hepatocellular carcinoma cell lines ( MHCC97,SMMC7721) ,HCC tissues,cancer organizations and normal liver by real-time PCR. Result The expression of miR-145 in hepatocellular carcinoma cell lines ( MHCC97,SMMC7721) is significantly lower than the human embryonic hepatocytes ( 0. 312 ± 0. 025,0. 396 ± 0. 076,0. 954 ± 0. 037, P ＜ 0. 05), and compared with the cancer organizations and normal liver,the expression of miR-145 in HCC tissues is significantly decreased (0. 162 ±0.002,0. 972 ±0.054,0.956 ±0. 018,P＜0. 05). Conclusion These results indicate that the miR-145 probably involved in HCC pathogenesis and was the indicator of diagnosis and prognosis of the HCC.     

SLC35F2在非小细胞肺癌中的表达及意义

Objective To evaluate the expression of Homo sapiens solute carrier family 35 member F2 (SLC35F2) in non-small-cell lung cancer (NSCLC). Methods Fifty-two cases of NSCLC and corresponding adjacent normal lung tissues were obtained by surgery in our center. Forty-three cases of the tissues were treated with Trizol as traditional method. Nine cases of the tissues were made into frozen slides firstly, then the carcinoma cells were separated by laser capture microdissection respectively from NSCLC tissues. The expression of SLC35F2 was detected by the method of quantitative real-time polymerase chain reaction. Results In both two groups, the data demonstrated that SLC35F2 expression was significantly up-regulated in NSCLC cells and tissues as compared with corresponding adjacent normal lung tissues ( NSCLC 2.460 ±0. 970 vs Normal 0. 006 ±0. 001 ,P <0. 05). The tumor pathology stage had a correlation with the expression of SLC35F2 ( Early stage 0. 30 ± 0. 14 vs Advanced stage 4. 80 ± 1. 93,P < 0. 01). Conclusion SLC35F2 was a potential oncogene of NSCLC.     

膝关节后交叉韧带断裂治疗临床分析

目的 对35例膝关节后交叉韧带断裂治疗进行临床分析,重点探讨了有关交叉韧带断裂的治疗问题。方法 经明确诊断后,分析采用胫骨附着处撕脱骨折复位固定手术治疗26例、早期髌韧带中1／3移植重建3例、单纯长腿石膏固定6例。结果 本组病例全部进行随访,随访时间13个月－5年,胫骨附着处撕脱骨折复位固定及髋韧带中1／3移植重建29例为优良、单纯长腿石膏固定6例为差。结论 后交叉韧带断裂后应该及时给予手术修复;膝后外侧手术入路,操作简单,暴露充分;少于3个月的陈旧性病例仍适应手术治疗。     

不同方法重建指尖离断静脉回流的疗效分析

目的：探讨不同方法重建指尖离断静脉回流的疗效。方法：2008年3月-2013年2月收治指尖离断患者80例,38例吻合指侧方静脉重建回流,术中吻合动静脉比例1：1或1：2或2：2,平均1：2;22例吻合指腹静脉重建回流,术中吻合动静脉比例1：1;20例未吻合静脉,术中仅吻合1条动脉,行侧切口或甲床放血。观察各组治疗效果。结果：吻合指侧方静脉组手指全部成活,无一例发生回流障碍;吻合指腹静脉组19例发生静脉危象,其中4例手指坏死;未吻合静脉组20例均发生回流障碍,其中6例手指坏死。58例获随访,随访时间6~28个月。吻合指侧方静脉组32例,指尖外形佳、指腹饱满;吻合指腹静脉组14例,指体轻度萎缩,指甲生长不平整;未吻合静脉组12例,指体萎缩明显。吻合指侧方静脉组指甲生长近平整,长度长于其他两组[（14.4±3.2）mm比（12.5±2.3）mm和（12.2±2.2）mm],远侧指间关节活动度大于其他两组[（63±5）°比（48±3）°和（45±7）°],两点分辨觉小于其他两组[（4.6±0.4）mm比（7.1±1.2）mm和（7.3±0.6）mm],感觉级别高于其他两组[S（3.45±0.39）级比S（2.57±0.42）级和S（2.55±0.49）级],差异均具有显著性（P〈0.05）。吻合指腹静脉组和未吻合静脉组在指甲长度、运动和感觉方面差异无统计学意义（P〉0.05）。结论：吻合指侧方静脉能有效解决指尖再植静脉回流问题,可避免回流障碍,成活率高,促进指甲生长,可恢复 DIPJ 活动度及感觉。     

胫骨干骨折的手术治疗对策

目的：明确不同固定器械在胫骨干不同骨折类型固定中的特点，以指导临床应用。方法：68例胫骨干骨折，行加压钢板螺钉、交锁髓内钉、单侧外固定架固定后，作临床疗效分析。结果：加压钢板固定组42例，感染5例，骨不连1例，平均愈合时间3．8个月；交锁髓内钉固定组13例，无感染及骨不连，平均愈合时间5．4个月；单侧外固定架组13例，骨不连1例，踝关节背伸受限3例，平均愈合时间4．5个月。结论：胫骨骨折交锁髓内钉固定并发症少，功能恢复好，适用范围广，但要注意及时进行动力加压。加压钢板及外固定架固定应选择各自的最佳适应证，以达到理想的疗效。