角质细胞生长因子对口腔黏膜上皮细胞凋亡的作用研究

目的 研究不同浓度角质细胞生长因子（KGF）对口腔黏膜上皮细胞凋亡的作用,为探讨KGF在口腔黏膜病发生发展中的作用提供依据。方法 将不同浓度的KGF（对照组0 ng·mL-1,实验1组5 ng·mL-1,实验2组25 ng·mL-1,实验3组50 ng·mL-1）分别加入体外培养的口腔黏膜上皮细胞,培养12、24、48 h后,倒置显微镜下观察其对细胞形态的影响,并用流式细胞仪检测细胞凋亡情况,荧光实时定量检测细胞凋亡相关基因Bcl-2、Bax mRNA的表达水平。结果 1）实验组较对照组细胞贴壁明显,且48 h时实验3组细胞核仁明显。2）培养48 h时,4组之间的细胞凋亡率、Bcl-2 mRNA、Bax mRNA表达均有统计学差异,随着KGF浓度的增加,细胞凋亡率和Bax mRNA表达逐渐降低,Bcl-2 mRNA表达逐渐升高（P<0.05）。结论 KGF可通过上调Bcl-2 mRNA和下调Bax mRNA的表达抑制上皮细胞的凋亡。     

KGF对口腔黏膜上皮细胞中增殖相关基因PCNAmRNA的作用

目的：检测不同浓度角质细胞生长因子（KGF）对体外培养的口腔黏膜上皮细胞形态学及对上皮细胞增殖相关基因PCNAmRNA表达的影响。方法：体外培养的口腔黏膜上皮细胞加入含不同浓度KGF（0ng／mL,5ng／rnL,25ng／mL,50ng／mL）的D—KFSM,分别培养12h、24h、48h后观察细胞形态改变并用荧光实时定量检测各组细胞内增殖相关基因PCNAmRNA的表达。结果：①相同时间段实验组较对照组上皮细胞贴壁明显,培养48h实验3组（50ng／mL）较其他组细胞核仁明显;②12h时,实验组较对照组上皮细胞内PCNAmRNA表达增加,但各实验组间PCNAmRNA表达逐渐降低（P〈0．05）;③24h时实验组较对照组PCNAmRNA表达增加,但各实验组间无统计学差异护〉0．05）：④48h时,实验组较对照组PCNAmRNA表达增加,且呈剂量依赖性（P〈0．05）。结论：外源性KGF可上调口腔黏膜上皮细胞增殖相关基因PCNAmRNA的表达,且在不同时间段、不同浓度调控作用存在差异。     

角质细胞生长因子及其与牙周病的关系

角质细胞生长因子是近年来发现的有着重要生物学功能的生长因子,它属于具有肝素结合特性的成纤维细胞生长因子家族,由间充质细胞产生并特异性地作用于上皮细胞。角质细胞生长因子可刺激上皮细胞增殖、迁移、分化,在牙周袋的形成和药物性牙龈增生中可能起到重要的作用。本文就角质细胞生长因子的生物学特性及其与牙周病的关系作一综述。     

口腔扁平苔藓黏膜成纤维细胞KGF的表达水平检测

目的 检测体外培养的正常口腔黏膜与口腔扁平苔藓黏膜成纤维细胞表达和分泌的角质细胞生长因子(KGF)的量。方法 应用酶联免疫吸附剂测定(enzyme-linked immunosorbnent assay,ELISA)技术检测体外培养的14例口腔扁平苔藓黏膜成纤维细胞和11例正常口腔黏膜成纤维细胞分泌的角质细胞生长因子蛋白的情况。提取各组细胞RNA,用逆转录-聚合酶链反应(RT-PCR)法检测KGF mRNA表达量的变化。结果 ELISA测定结果显示,扁平苔藓黏膜成纤维细胞分泌角质细胞生长因子蛋白量低于正常口腔黏膜,经检验差异有统计学意义(P0.05)。结论 与正常口腔黏膜相比,口腔扁平苔藓黏膜成纤维细胞分泌的角质细胞生长因子蛋白水平降低,而在基因水平上没有变化。     

白细胞介素-1β与地塞米松对成纤维细胞分泌角质细胞生长因子的影响

目的 检测在体外培养的人正常口腔黏膜及口腔扁平苔藓(OLP)黏膜成纤维细胞中加入不同浓度的人白细胞介素-1β(IL-1β)和地塞米松(DEX)后,对成纤维细胞分泌和表达角质细胞生长因子(KGF)的影响.方法 将IL-1β和DEX配制成3个浓度梯度,分别加入到体外培养的人正常口腔黏膜和OLP黏膜成纤维细胞中,收集培养72 h后各组细胞上清液,应用酶联免疫吸附剂测定(ELISA)技术检测每组细胞上清液中KGF蛋白浓度的变化.提取各组细胞RNA,用聚合酶链反应(PCR)法检测KGF mRNA表达量的变化.结果 ELISA及PCR结果显示:加入IL-1β的正常口腔黏膜及OLP黏膜成纤维细胞培养上清液中,KGF蛋白浓度较自身对照组均升高,同时KGF mRNA表达量较对照组有较明显的上升趋势.正常口腔黏膜及OLP黏膜成纤维细胞加DEX的上清液中,KGF蛋白浓度较自身对照组均降低,KGFmRNA表达量较对照组则有降低的趋势.结论 IL-1β对正常口腔黏膜成纤维细胞及OLP黏膜成纤维细胞分泌及表达KGF有促进作用;而DEX对正常口腔黏膜成纤维细胞及OLP黏膜成纤维细胞分泌和表达KGF有抑制作用.     

FGF-2对体外培养人牙髓细胞FGFR和OPN表达活性的影响

目的:研究成纤维细胞生长因子-2(fibroblast growth factor-2,FGF-2)对体外培养的人牙髓细胞表面成纤维细胞生长因子受体(fibroblast growth factor receptor,FGFR)及骨桥蛋白(osteopontin,OPN)表达的影响。方法:以改良组织块培养法体外获得人牙髓细胞,采用Western blotting法检测不同浓度FGF-2作用下牙髓细胞FGFR的表达情况;应用Real Time-PCR法,检测不同浓度FGF-2作用下牙髓细胞OPN mRNA的表达。结果:与对照组相比,FGF-2在1～50 ng/mL浓度下均可促进牙髓细胞FGFR的表达(P<0.05),最佳显效浓度为10 ng/mL。FGF-2在1～50 ng/mL浓度下均可诱导牙髓细胞OPN mRNA表达(P<0.05),OPN mRNA的表达在10 ng/mL的FGF-2作用下达到高峰。结论:FGF-2可促进体外培养人牙髓细胞FGFR和OPN的表达,在牙髓牙本质复合体修复中可能发挥重要作用。     

白细胞介素-1β与地塞米松对成纤维细胞分泌角质细胞生长因子的影响

目的 检测在体外培养的人正常口腔黏膜及口腔扁平苔藓(OLP)黏膜成纤维细胞中加入不同浓度的人白细胞介素-1β(IL-1β)和地塞米松(DEX)后,对成纤维细胞分泌和表达角质细胞生长因子(KGF)的影响.方法 将IL-1β和DEX配制成3个浓度梯度,分别加入到体外培养的人正常口腔黏膜和OLP黏膜成纤维细胞中,收集培养72...     

Wnt1、β-catenin在硝苯地平引起的药物性牙龈增生中的表达

目的:探讨Wnt/β-catenin信号通路相关蛋白Wnt1、β-catenin在硝苯地平引起的药物性增生的牙龈组织中的表达.方法:选择正常牙龈对照组、(未服药)高血压牙龈增生组、硝苯地平引起的药物性牙龈增生组各10例,用Western-Blot和RT-PCR检测牙龈组织中Wnt1、β-catenin的表达.结果:药物性牙龈增生组的牙龈组织中Wnt1、β-catenin蛋白及mRNA表达水平显著高于正常牙龈对照组(P＜0.05)和高血压牙龈增生组(P＜0.05).结论:硝苯地平引起的药物性牙龈增生的牙龈组织中Wnt1、β-catenin表达水平增高,可能通过Wnt/β-catenin信号通路促进牙龈成纤维细胞的增殖导致牙龈纤维化.     

服用硝苯地平后牙龈增生以及未增生患者牙龈成纤维细胞生物特性比较

目的：观察服用硝苯地平后牙龈增生和未增生患者牙龈成纤维细胞（nifedipine responders gingival fibro-blasts NIFr-HGF,nifedipine non-responders gingival fibroblasts NIFn-HGF）超微结构、细胞周期变化以及增殖能力的差异性,以探讨该药导致牙龈增生的可能作用机理。方法：采用透射电镜观察NIFr-HGF、NIFn-HGF的超微结构,利用流式细胞仪、MTT法检测和比较2种细胞经硝苯地平诱导后其细胞周期以及增殖能力的差异性。结果：与NIFn-HGF相比较,NIFr-HGF内粗面内质网扩张;受硝苯地平诱导后其增殖明显加强。结论：NIFr-HGF合成蛋白质的能力可能较NIFn-HGF强,且前者对于硝苯地平的反应也明显强于后者,这提示两类细胞的细胞生物学特性以及对钙离子拮抗剂的反应能力存在差异,药物性牙龈增生的发生可能存在细胞异质性。     

表皮生长因子对Balb/3T3成纤维细胞生长周期及表皮生长因子受体表达的影响

目的:观察表皮生长因子(Epidermal growth factor,EGF) 对Balb/3T3成纤维细胞生长周期及表皮生长因子受体(Epidermal growth factor receptor,EGFR)表达的影响.方法:①通过血清饥饿法使细胞同步化,加入25 ng/ml的EGF予以刺激,用流式细胞仪检测不同时间细胞生长周期参数的改变;②于不同时间点对Balb/3T3细胞进行EGFR免疫组化染色,观察EGF对EGFR表达的调节作用. 结果:①Balb/3T3细胞在加入EGF 8 h后S期细胞比例及增殖指数达到最高峰,至10 h回落至原水平;②EGF在作用早期可促进成纤维细胞EGFR表达,其对受体表达的调节作用与正常培养液相当.结论:EGF可有效促进EGFR的表达,从而促进细胞增殖.     

Evidence of the validity of a model of determinants of quality of restorative dental care

A model describing the relationship between self-reported quality of restorative dentistry and dentist characteristics for 119 Montana general dentists is presented. The best predictors formed a significant model explaining 22% of the variance of the quality measure. Results are contrasted with a previous estimation of the model for 102 Washington general practitioners. Evidence for the external validity of the model is presented.     

Experimental evidence of formation of imines in the course of reduction of hydrazones

The reduction of hydrazones is generally suggested to proceed through a reductive cleavage of the nitrogen–nitrogen bond followed by a reduction of the carbon–nitrogen bond. This sequence of reduction processes is here supported for fluorenone (V) and benzophenone (VI) hydrazones as well as by a comparison of the reduction of fluorenone and benzophenone hydrazonium ions (I,III) with corresponding imines (II,IV). Another proof of the presence of imines as intermediates is the splitting of four-electron waves of hydrazones V and VI and hydrazonium ions I and VIII into two waves at pH < 2. This has been interpreted as due to differences in slopes dE_1/2/dpH and pK_a-values of protonated hydrazine derivatives on one side and corresponding imines on the other. In this pH-range imines formed in reductions of VI and VIII are reduced in a single two-electron wave, those of I and V in two one-electron steps. Fluorenone imine (II) is sufficiently stable to allow recording of time-independent current–voltage curves between pH 6 and 11. In this pH-range the imine (II) is reduced in two one-electron steps. Benzophenone imine (IV) has been found stable between pH 4.6 and 12. At pH 4.6–8 the reduction of the imine IV takes place in a single two-electron step, at pH 8–12 in two one-electron steps. Final proof of the initial cleavage of the N–N bond is presented by comparison with the reduction of nitrones.     

不同剂型玻璃离子水门汀溶解性比较研究

目的:研究、比较不同剂型玻璃离子水门汀的溶解性和表面微观形态改变,为临床使用提供依据.方法:将3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)及GC玻璃离子水门汀(双糊剂型)分别在人工唾液中浸泡30 d,冷热循环15000次,烘干测重,比较前后质量变化,计算溶解率,并用扫描电镜观察表面微观改变.结果:不同剂型的玻璃离子水门汀溶解率由高到低分别为3M树脂加强型玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(水粉剂型)、GC玻璃离子水门汀(双糊剂型).3种玻璃离子水门汀经浸泡溶解后,SEM扫描表面微观形态可观察到GE玻璃离子水门汀(双糊剂型)表面形态改变较少,其他2组玻璃离子水门汀表面微观改变较多.结论:双糊剂型玻璃离子水门汀理化性能及溶解率均低于传统水粉剂型,是未来临床修复治疗的的良好选择.     

Design of clinical trials of traditional therapies of periodontitis

The present paper on the design of clinical trials of periodontal therapy first addresses the issue of the etiology of periodontal disease. It is suggested that most if not all forms of destructive periodontal disease are caused by microorganisms and that there are different forms of disease with different microbial etiologies. The progressive nature of destructive periodontal disease is subsequently discussed and it is emphasized that, in a given patient, periodontal sites which show signs of inflammation and attachment loss may not over a period of several months and years show further sign of attachment loss. The present methods of assessing periodontal disease do not allow us to discriminate between potentially active and inactive sites in untreated patients. The significance and variability of indicators of periodontal disease such as bleeding on probing, probing pocket depth and probing attachment level measurements are discussed. The errors inherent in the various measurements are analyzed and suggestions are presented describing how alterations in any of the above parameters could be identified and presented in a clinical trial. Of concern for the statistical analysis of clinical data of periodontal disease is the definition of the "experimental unit". For a number of years, the "experimental unit" in periodontal trials was the patient. It is clear, however, that different sites within the same individual show different patterns of disease progression and lesion morphology and often respond differently to periodontal therapy. Statistical analyses must consequently be designed which recognize differences in site-to-site infection and lesion morphology within a common host. Until such analyses are available, the investigator should be wary of pooling data within the same individual, since such pooling may obscure meaningful alternatives which may take place in individual periodontal sites. Some goals of periodontal therapy are subsequently identified. 4 goals are discussed more in detail, namely: to establish conditions which will allow the patient to maintain a dentition without further breakdown of the periodontium; to reduce pocket depth to establish an anatomy in the dentogingival region which with proper maintainance care will prevent the re-establishment of the subgingival infection; to gain attachment as a result of treatment; to assess the effect of a certain chemotherapeutic agent on periodontal disease.     

Evaluation of efficacy of chemiluminescence for diagnosis of leukoplakia

ObjectiveLeukoplakia is the most common potentially malignant disorder preceding oral cancer. Chemiluminescence has been developed as an adjunct to conventional examination for the diagnosis of these potentially malignant disorders. This study was conducted to assess the efficacy of chemiluminescence in the diagnosis of leukoplakia and to compare the results with histopathological examination.Study designA total of 50 patients with leukoplakia were included from the outpatients attending the Department of Oral Medicine and Radiology, Dental Hospital, Bengaluru, Karnataka, India. These patients were subjected to conventional oral examination followed by chemiluminescent examination with Vizilite (Zila, Fort Collins, CO, USA) and biopsy for histopathological confirmation.ResultsThe sensitivity, specificity, positive predictive value, and negative predictive value of chemiluminescence were 93.75%, 55.56%, 78.95%, and 83.3%, respectively. The overall accuracy of chemiluminescence was 80%. A statistically significant association was observed between histopathology results and chemiluminescence results.ConclusionAlthough it is an easy, safe, minimal time consuming, and noninvasive technique, it has only adjunctive utility and it does not replace biopsy for the diagnosis of leukoplakia.     

颌骨动静脉畸形的栓塞治疗

目的：总结直接穿刺结合经血管内介入栓塞治疗颌骨动静脉静脉畸形的经验。方法：收治凳骨动静脉畸形患者6例,均进行了介入栓塞治疗。采用的栓塞材料为附凝血棉纤毛的螺圈,聚乙烯醇泡沫微粒和二氰基丙烯酸对丁酯。数字减影颈动脉造影在PHILIPSV300下完成。结果6例颌骨动静脉畸形患者中4,例急性出血得到了快速、有效控制,1例慢性渗血的右下 骨动静脉畸形患者,介入栓塞治疗,拔除松动的右下凳第一磨牙,有效地控制了出血,另1例伴局部软组织搏动性膨隆的上凳骨动静脉畸形患者,介入治疗后膨隆的搏动性得到明显改善,栓塞治疗后分别随访3－24个月,均未发现有口腔内渗血或出血。随访的X线片上,病灶区可见新骨形成。结论：局部穿刺结合经血管内介入栓塞治疗颌骨动静畸形是一种安全、有效的治疗方法。     

正畸患者切牙影像失真发生情况分析

目的研究正畸患者曲面体层片上的切牙影像失真发生情况，并分析其原因。 方法从中山大学附属口腔医院放射科影像数据库中选取500例正畸患者的曲面体层片和头影测量侧位片，所有曲面体层片均采用咬合杆投照，分别从切牙牙体影像放大、缩小、牙根变短、根尖模糊等评价指标分析上下颌切牙影像失真的发生情况，在头影测量侧位片上测量中切牙根尖-对颌切牙切缘的距离，探讨切牙影像失真发生的原因。采用SPSS 19.0统计软件对所得数据进行统计学检验。 结果500例患者中，切牙牙体影像正常者共417例，切牙牙体影像失真者共83例，影像失真发生率16.6%，其中切牙牙体影像放大17例、牙体影像缩小0例、牙根变短30例，牙根影像变短伴模糊36例。影像失真患者的根尖-切缘距离大于影像正常的患者，差异有统计学意义（F = 5 187.18，P = 0）；影像失真患者的覆盖值大于影像正常的患者，差异有统计学意义（F>477，P = 0）。 结论严重牙颌面畸形如反 、深覆盖是导致曲面体层片的切牙影像失真的主要原因之一。     

正常青年Monson球面半径的测量研究

目的测量正常青年Monson球面半径。方法选择60名(男30名,女30名)正常青年制取全口印模,应用立体摄影成像的原理与方法对Monson球面半径进行测量和统计学处理。结果Monson球面的半径平均为10.173 cm,大于理论值10.160 cm,差异有显著性(P<0.01);男、女性球面半径差异无显著性。结论本实验所得到的数据可作为全口义齿修复中记录颌位关系的一个参量。     

鼻测量法的进展

唇裂术后继发畸形是指唇裂修复术后,仍遗留或继发于手术操作和生长发育变化而表现出来的一类畸形[1]。包括唇畸形、鼻畸形和颌骨畸形。其修复较原发性唇裂修复更复杂,更灵活多变。而导致其修复复杂性的一个重要原因即是局部组织结构复杂变异和缺乏可靠的三维测量手段[2],鼻畸形