子宫肌瘤雌二醇胞浆受体含量与血浆及组织激素水平关系的探讨

作者测定了36例子宫肌瘤患者子宫组织ER、E_2和P含量及血浆E_2、P含量,观察了丙酸睾丸酮对肌瘤ER的影响。肌瘤组织ER含量37.6±4.0 fmol/mg蛋白,E_2含量401.7±92.6pg/g组织,两者均高于同一子宫正常肌组织的相应值(P<0.05)。11例肌瘤患者用丙酸睾丸酮后肌瘤组织ER含量(18.9±54 fmol/mg蛋白)较未用药组的有显著降低(P<0.05)。本实验结果表明肌瘤组织中ER及E_2水平的改变与肌瘤生长有关,丙酸睾丸酮抗肌瘤生长的机理可能与降低肌瘤组织中的ER水平有关。     

女性男性化疾病的内分泌测定

本文对51例男性化妇女测定了睾丸酮、游离睾丸酮、雄烯二酮、17α-羟孕酮、硫酸脱氢表雄酮(DHAS)、性激素结合蛋白(SHBG)、雄甾烷二醇和游离雄激素指数(FAI)。男性化妇女伴有高雄激素者92％有明显的SHBG值降低,且FAI明显升高,雄烯二酮和17α-羟孕酮无明显变化,17例雄甾烷二醇值均高于正常,且与睾丸酮和游离睾丸酮有密切相关性(γ=0.78,γ=0.80)。对于女性男性化病例的诊断除测定睾丸酮和DHAS外,还应测定SHBG和FAI。     

二-(2, 4-二氯苯甲酰异羟肟酸) 二苯基合锡大鼠血浆蛋白结合率的测定

目的 建立二-(2, 4-二氯苯甲酰异羟肟酸) 二苯基合锡（DPDCT）在大鼠血浆中蛋白结合率的测定方法。方法 采用HPLC法测定大鼠血浆中药物总浓度及游离药物浓度,结合平衡透析法测定血浆蛋白结合率。结果 DPDCT在质量浓度为0.2、1.0、5.0 μg/mL时与大鼠的血浆蛋白结合率分别为（58.9±2.0）%、（57.4±1.2）%和（58.6±0.8）%。不同浓度组血浆蛋白结合率差异无统计学意义。结论 该方法灵敏度高,重复性好,操作简单,能够满足生物样品分析的要求。DPDCT具有中等强度的血浆蛋白结合率。     

黄龙舒喘颗粒对哮喘豚鼠血浆溶血血小板激活因子(Lyso-PAF)的影响

目的:探讨黄龙舒喘颗粒对哮喘豚鼠血浆溶血血小板激活因子的影响。方法:用10%卵清蛋白行豚鼠腹腔注射,2w后用1%卵清蛋白雾化吸入,每d1次,连续5d。同时,分别用生理盐水、抵克立得、黄龙舒喘颗粒干预。取动脉血测定Lyso-PAF的含量。结果:生理盐水组Lyso-PAF为(89.59±6.96)ng/ml,抵克立得组Lyso-PAF为(60.20±2.18)ng/ml,黄龙舒喘颗粒组Lyso-PAF为(70.78±2.50)ng/ml。三者比较,P<0.01。结论:黄龙舒喘颗粒能显著降低哮喘豚鼠模型Lyso-PAF的含量     

葡萄糖酸钙对链霉素过敏休克的保护作用及其机理

葡萄糖酸钙对链霉素—蛋白(ST-BSA)致敏豚鼠过敏休克的保护作用的实验研究,结果对照组一般过敏反应发生率为27%,休克率为53%,死亡率为20%;保护组一般过敏反应率为13%,无休克和死亡发生。致敏豚鼠离体回肠实验结果,对照组肠管收缩高度为48.7±10.8mm,保护组为2.2±4.1mm,两组差异显著(P<0.01);ST-BSA致敏家兔血清异种动物PCA实验结果,对照组抗体滴度为1:4096,原血清蓝斑直径为35.9±1.9mm,保护组抗体滴度为1:512,原血清蓝斑直径为25.2±0.8mm,两组抗体滴度及蓝斑直径差异显著(P<0.01);大鼠腹腔肥大细胞脱颗粒实验结果,致敏对照组脱颗粒率为54.3±7.1%,保护组为17.5±6.8%,两组结果有明显差异(P<0.01)。提示葡萄糖酸钙对ST-BSA致敏豚鼠过敏休克有保护作用,其机理可能与抑制肥大细胞脱颗粒释放组胺等过敏介质有关。     

PTX-3在干燥综合征患者中的表达水平及其临床意义

目的:探讨正五聚素-3(pentraxin 3,PTX3)在干燥综合征中的临床意义?方法:ELISA方法检测52例干燥综合征患者和15例健康体检者血浆PTX3水平,并分析了PTX3与干燥综合征患者临床指标的相关性?结果:①血浆PTX3水平变化:干燥综合征患者血浆PTX3水平[(1.492 ± 0.018)ng/mL]显著高于健康对照组[(1.403 ± 0.019)ng/ml],其中肾小管功能异常患者血浆PTX3水平[(1.573 ± 0.019)ng/mL]显著高于无肾小管功能异常患者[(1.418 ± 0.003)ng/mL](P < 0.01)?②在干燥综合征合并肾小管功能异常的患者中,血浆PTX3水平与尿可滴定酸及尿铵离子水平呈显著负相关(P < 0.05),而与炎症相关指标C反应蛋白?血沉无明显相关性,与免疫学指标IgA?IgG?IgM?SS相关抗体?类风湿因子无相关关系?③对于干燥综合征合并肾小管功能异常的诊断效能PTX3与尿β2微球蛋白相当,PTX的ROC曲线下面积(AUC)为0.702(95%CI:0.593~0.811),与尿β2微球蛋白(AUC:0.705,95%CI:0.567~0.843)无明显差异?结论:PTX3可能作为干燥综合征患者肾小管间质损伤的生物学标志物?     

SARS患者血浆纤维结合蛋白水平变化及其意义

目的:探索急性期SARS患者和康复期SARS患者血浆纤维结合蛋白(plasma Fibronectin,Fn)的水平变化及输注血浆Fn纠正治疗急性期SARS患者的可行性。方法:急性期SARS组为4名住院的急性期SARS患者;康复期SARS组为111名康复期SARS住院患者;对照组为随机26名体检合格的无偿献血者。采用ELISA法测定各族组血浆Fn水平。结果:急性期SARS组血将Fn浓度为(42.2±23.0)mg/L;恢复期SARS组血浆Fn浓度为(379.45±93.27)mg/L;对照组血浆Fn浓度为(359.21±52.54)mg/L。急性期SARS组血浆Fn浓度显著低于对照组(P=0.001);急性期SARS组与恢复期SARS组血浆Fn浓度差异极显著(P=0.000);恢复期SARS与对照组血浆Fn浓度无显著差异(P=0.289)。结论:急性期SARS患者血浆纤维结合蛋白水平显著降低,为输注外源性的纤维结合蛋白治疗SARS提供了依据。SARS患者血浆Fn水平的变化可能是辅助诊断和判断SARS病情的指标之一。     

地塞米松对哮喘豚鼠肺组织Giα、Gqα的抑制作用

目的　研究糖皮质激素对豚鼠哮喘模型肺组织G蛋白α亚族表达的影响。方法　 18只豚鼠随机分为正常对照组(NS) ,哮喘组 (AS)和地塞米松治疗组 (DEX)。AS组用卵蛋白皮下及腹腔注射致敏 ,卵蛋白重复雾化吸入激发复制豚鼠哮喘模型。DEX组在每次激发前腹腔内注射地塞米松 2mg/kg进行干预。用Westernblot分析法测定豚鼠肺组织G蛋白α亚族的表达水平。结果　三组豚鼠肺组织Giα水平分别为NS组 10 0 % ,AS组 (15 3± 18) % ,DEX组 (113± 18) % ,AS组与NS组比较、DEX组与AS组比较差别显著 (P <0 .0 5 )。三组豚鼠肺组织Gqα水平分别为NS组 10 0 % ,AS组 (15 1± 19) % ,DEX组 (98± 4 ) % ,AS组与NS组比较、DEX组与AS组比较差别显著 (P <0 .0 5 )。在同样的条件下Gsα水平无显著变化 (P >0 .0 5 )。地塞米松能够显著抑制哮喘豚鼠肺组织中Giα、Gqα的高表达。结论　肺组织Giα ,Gqα的高表达变化参与了豚鼠哮喘模型的病理性信号传导 ,抑制哮喘肺组织Giα、Gqα的高表达可能是糖皮质激素治疗哮喘的机制之一。     

青霉噻唑蛋白致敏豚鼠过敏休克及肺组胺释放的研究

用青霉噻唑(BPO)蛋白致敏豚鼠并诱发过敏休克反应,休克率与休克死亡率均为100%(10/10);致敏组与对照组豚鼠肺组织经抗原攻击后组胺释放率分别为12.8±3.5%和1.0±0.2%,未经抗原攻击者分别为0.7±0.2%和1.0±0.4%;被动致敏皮肤过敏反应(Passive cutaneousanaphy laxis,PCA)实验证明,致敏豚鼠血清中有抗BPO IgG抗体存在,滴度为64～128,并有种属特异性。表明致敏豚鼠过敏休克的发生与组织胺释放和血清抗BPO抗体产生有关。     

地塞米松对豚鼠哮喘模型肥大细胞和类胰蛋白酶的影响

目的:探讨地塞米松对豚鼠哮喘模型肺组织中肥大细胞及血浆类胰蛋白酶含量的影响。方法:将30只雄性豚鼠分为哮喘模型、地塞米松治疗和健康对照组,用卵白蛋白(OVA)腹腔注射致敏和雾化吸入激发建立哮喘豚鼠模型。镜下观察肺组织肥大细胞数量及脱颗粒现象。运用UniCAP100全自动体外变应原检测仪测定豚鼠血浆类胰蛋白酶含量。结果:哮喘模型组豚鼠肺高倍视野肥大细胞数、脱颗粒百分率及血浆类胰蛋白酶含量分别为14.87±4.67个,42.85±5.99%,119.78±12.50均明显高于对照组(2.38±0.52个,24.91±9.79%,88.78±4.74,P<0.01);地塞米松治疗组豚鼠肺高倍视野肥大细胞数、脱颗粒百分率及血浆类胰蛋白酶含量分别为6.63±1.41个,25.33±6.14%,92.10±7.11,显著低于哮喘模型组(P<0.01)。结论:肥大细胞类胰蛋白酶参与了哮喘的致病过程;而糖皮质激素在哮喘中的抗炎作用可能是通过抑制肥大细胞类胰蛋白酶的释放而实现的。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.