NEOPLASTIC NATURE OF ARGYROPHIL CELLS IN URACHAL ADENOCARCINOMA

The histological, histochemical and electron microscopic features of themetastatic tumors of an urachal adenocarcinoma, were presented. Metastatic tumor nodules in the lungs and brain as well as the primary tumor showed tubular adenocarcinoma containing many argyrophil cells. immunoperoxidase examination revealed three kind of endocrine cells which contained different endocrine hormones. Electron microscopic examination showed small, round endocrine granules in the endocrine cells and desmosome-like complexes in between these cells and the adjacent glandular neoplastic cells. These findings suggested that the endocrine cells were neoplastic in nature and originated from primitive neoplastic cells as well as glandular neoplastic cells. ACTA PATHOL. JPN. 36: 1587-1592, 1986.     

胎儿胃粘膜内分泌细胞免疫组化的定量研究

应用免疫组化方法对56例胎儿、5例新生儿、5例成人尸体解剖正常胃标本的内分泌细胞进行定量观察.第一抗体包括一嗜铬粒蛋白A、胃泌素、胃体抑素、五羟色胺、降钙素、绒毛膜促性腺激素、促肾上腺皮质激素、胰多肽、胰高血糖素、蛙皮素.应用图像分析系统对各类内分泌细胞进行定量测量.结果发现人胎儿胃内分泌细胞每平方厘米计数高于成人,尤其D细胞明显高于成人组(P<0.01).G细胞、D细胞、EC细胞、P细胞及CT阳性细胞分布与成人相似.在胎儿胃中还可见到成人胃中很少见到的绒毛膜促性腺激素、胰多肽、胰高血糖素阳性细胞.     

ARGYROPHIL CELLS IN THE EXOCRINE PANCREAS

The silver positive cells of the exocrine pancreas and primary pancreatic cancers were studied with the Grimelius silver stain and the Fontana-Masson technique. In the pancreas, cells containing black granules with the Grimelius method, which at the same time react negative to Fontana-Masson, are considered argyrophil. These cells were present in the basal portion of some of the acinar tissue and in the ductal epithelia, as well as in the A cells of islets. The incidence and distribution of these argyrophil cells were also studied in a variety of ductal lesions. In the so-called ductal proliferation numerous numbers of positive cells were found. Argyrophil cells were frequently situated in the basal portion of ductal squamous cell metaplasia. In goblet cell metaplasia the numbers were few, and less than in normal ducts. We concluded that the distribution and incidence of argyrophil cells in the ductal epithelia is related to chronic pancreatitis, and in particular to regenerative processes. The incidence of argyrophil cells in primary pancreatic cancer, excluding islets cell origin, was 18 per 41 cases (43.4%). We considered them pancreatic cancer with argyrophil cells. ACTA PATH. JAP. 29 : 413–419, 1979.     

GASTRIC CARCINOIDS OF ECL CELLS

Clinical, histological, histochemical and ultrastructural characteristics of eight cases of carcinoid tumors of the non-antral portion of the stomach are presented. Four cases with multiple polypoid lesions are accompanied by an increased level of gastrin. A normal level of gastrin was present in the other four cases with isolated tumor and a normal component of endocrine cells in the uninvolved mucosa. In the first group with multiple lesions, the histological and histochemical analysis of the endocrine cells revealed a wide range of appearances: a) "simple hyperplasia", b) "nodular hyperplasia", and c) carcinoid tumor. These aspects suggested a different pathogenesis for the carcinoid tumors of the non-antral portion of the stomach with possible therapeutical implications. ACTA PATHOL. JPN. 35: 361–375, 1985.     

大鼠实验性胃溃疡自愈过程中颌下腺颗粒曲管细胞的形态变化

用半薄切片甲苯胺蓝染色方法，观察大鼠实验性胃溃疡自愈过程中颌下腺颗粒曲管（ＧＣＴ）细胞的变化。ＧＣＴ细胞可分为三种类型：宽暗颗粒细胞、亮颗粒细胞和狭窄玩颗粒细胞。溃疡术后４天，宽暗颗粒细胞和颗粒明显减少；术后１０天，宽暗颗粒细胞和颗粒略增多；术后１４－２１，宽暗颗粒细胞和颗粒均明显增多，颗粒更加粗大，含颗粒少的细胞向含颗粒多的细胞过度明显，这些变化尤以术后１４天更明显；至术后２８天，各项变化趋于恢     

大鼠胃肠嗜银细胞的表皮角蛋白免疫细胞化学定位

本文报道应用表皮角蛋白(EK)抗体,对大鼠胃肠粘膜上皮进行了免疫细胞化学定位。应用PAP和间接免疫荧光染色技术,观察到除柱状细胞、杯状细胞呈EK阳性外,还有一种EK强阳性且在上皮内的位置以及形态酷似嗜银细胞的上皮细胞。应用浸银-PAP联合染色法显示,嗜银细胞内确实存在着EK阳性物质,位于嗜银细胞的外周部位和嗜银颗粒之间。提示胃肠嗜银细胞可能与其他胃肠粘膜上皮细胞一样,含有细胞角蛋白型中间丝(IF)。本研究的结果支持胃肠嗜银细胞起源于内胚层的假说。     

HISTOPATHOLOGICAL STUDY OF EARLY LESIONS OF PANCREATODUODENAL CANCER

The early lesions of pancreatoduodenal cancer, which was defined as those in which the site of origin is confined in a single certain anatomical part and the mode of early extension can be pursued, were found in 28 of 52 surgical specimens examined; 12 in the pancreatic portion of the common bile duct (PCBD), 12 in the common channel, 1 each in the terminal portion of the common bile duct and the terminal portion of the pancreatic duct, and 2 in the peripapillary zone of the duodenum. There were roughly two types of tumor growth; one annular flat-raised, histologically scirrhous tubular adenocarcinoma, principally found in PCBD and the other polypoid, histologically papillary or papillotubular adenocarcinoma, found everywhere. The staining properties of mucin was of no value in differential diagnosis for the site of origin. One typical carcinoid was observed in PCBD. The surface spread of carcinoma beyond the originating part was seen in 10 cases (36%), lymphatic invasion in 23 cases (82%), node metastasis in 9 cases (32%), and venous invasion in 6 cases (21%). Only 2 cases were mucosal carcinomas. From these figures it can be concluded that the carcinoma of this region is of high grade malignancy.     

HISTOPATHOLOGICAL STUDY OF SIX CASES OF CASTLEMAN'S TUMOR

A histopathological analysis of six cases of Castleman's tumor by means of light and electron microscope was performed, with a review of literature. All cases were hyaline-vascular type as described by Keller et al. The morphology of lymphoid follicles in the lesions varied according to the presence or non-presence of the germinal center which was from large active to emaciated hyalinized. The lymphoid follicle was essentially similar to that of normal lymph node undergoing some reactive process. Depending on the observation of serial sections, the lesions had lymphatic sinuses around the blood vessels in the tumor parenchyma, some of which were proved to be connected to the abortive marginal sinuses.
These findings and some clinical records suggest that the lesion originates from the lymph node and is a result of its reactive hyperplasia.     

大鼠小肠嗜银、亲银细胞的分布及形态学观察

本文用肠卷石蜡切片的嗜银反应(黄荫乔法)、亲银反应(Singh法),对11只大鼠小肠的嗜银、亲银细胞的分布及形态学作了初步观察,结果如下。 1.大鼠小肠的嗜银、亲银细胞的密度,在十二指肠最高,从空肠到回肠逐渐减少。 2.嗜银、亲银细胞在肠腺基底部着色较浅,在腺上部着色加深,在绒毛顶端为深染。嗜银细胞基底部有突起,穿过基膜到达固有层。在固有层内,于突起附近有嗜银颗粒和突起相延续。嗜银颗粒到达细胞顶端较为多见,有时可见到嗜银颗粒释放到腺腔内。因此我们认为,嗜银、亲银细胞兼有内、外分泌双重功能。 3.在小肠固有层的结缔组织内,发现有嗜银细胞,细胞形状不规则,有突起,胞质和突起都充满嗜银颗粒,有时可见嗜银颗粒到达细胞外。颗粒的形态、致密度及染色特点,与上皮细胞之间的嗜银细胞相同,故这些细胞可能属于内分泌细胞。     

小鼠十二指肠嗜银细胞发育分化的组织学观察

用Ｇｒｉｍｅｌｉｕｓ嗜银法研究了小鼠胚胎１４天至成年十二指肠嗜银细胞的发育分化过程。提示肠嗜银细胞可能有外分泌功能。     

HISTOPATHOLOGICAL CLASSIFICATION OF GASTRIC CARCINOMA FOR STANDARD REPORTING SYSTEM USED IN JAPAN

Analysis of approximately 2,000 cases of gastric carcinoma which survived for more than 5 years after gastrectomy revealed that the histopathological classification system proposed by the Japanese Society for Gastric Cancer Research had fair prognostic value and can be recommended for general use.     

大鼠实验性胃溃疡自愈期间胰岛PP细胞的免疫组织化学研究

目的 探讨胰多肽在大鼠胃溃疡自愈期间的可能作用及意义.方法 应用免疫组织化学PAP法、形态计量和图像分析法,研究大鼠胃溃疡自愈期间胰岛PP细胞的形态学、细胞的面数密度(NA)及平均灰度变化.结果 与正常组和盐水组比较,胃溃疡组大鼠胰岛PP细胞NA于溃疡第6d明显下降,平均灰度值升高(P＜0.05);溃疡第10～28d,PP细胞NA升高、平均灰度值下降(P＜0.05);尤以第10d最明显.胰岛PP细胞免疫组织化学的形态观察结果与同期图像分析结果基本一致.结论 胰岛PP细胞在实验性胃溃疡自愈期间,其细胞NA和平均灰度均发生改变.提示PP细胞参与了胃溃疡自愈的调节过程.     

THYMIC LYMPH FOLLICLES A HISTOPATHOLOGICAL STUDY OF 1,356 AUTOPSY CASES*

1) Eighteen cases with definite thymic lymph follicles were found among 1,356 autopsies. Eight of those were cases of Basedow's disease and the remaining 10 consisted of 2 cases of asthma syndrome one of which was associated with a type of allergic granulomatous angiitis, 1 case each of Wegener-like granulomatosis, periarteritis nodosa, rheumatoid arthritis, aplstic anemia, chronic glomerulonephritis, acute yellow liver atrophy, Ekiri syndrome and purulent necrotizing tracheo-bronchitis. 2) No morphological differences were found between the thymic lymph follicles and lymph follicles in other organs. The folliculated thymus did not indicate any specific morphological features. 3) Basically, in cases with thymic lymph follicles, the lymphatic tissue of other organs showed lymphatic follicular hypoplasia. 4) Speculative explanation of the histogenesis and significance of thymic lymph follicle formation was attempted. ACTA PATH. JAP. 16:109-130, 1966     

大鼠实验性胃溃疡自愈期间胰岛胃泌素和生长抑素细胞的免疫组织化学研究

目的　探讨大鼠实验性胃溃疡自愈期间 ,胰岛胃泌素免疫反应细胞 (gastrin ,G)和生长抑素细胞(somatostatin ,SSorD)的变化。　方法　免疫组织化学ABC技术。　结果　大部分G细胞免疫反应深浅不一 ;溃疡术后 4、10d ,胰岛G细胞面数密度增高 ,与正常或盐水组相比P <0 0 5。D细胞面数密度于 4d增加 ,P <0 0 5。　结论　成年大鼠胰岛细胞呈胃泌素免疫反应阳性 ;胰岛G细胞和D细胞可能以内分泌或旁分泌调节的途径间接或直接参与大鼠实验性胃溃疡修复的过程。     

PATHOLOGICAL STUDY OF MULTIPLE GASTRIC CARCINOMA

Multiple gastric carcinoma was investigated on 101 of 1901 resected stomachs. The frequency of multiple carcinoma was 4.7% and 6.9% of the advanced and early carcinomas, respectively. Male to female ratio was 2.9 to 1 with average age of 62.0 and that was 1.9 to 1 with average age of 57.7 in the total gastric carcinomas. The well-differentiated carcinomas with intimate relation to the intestinal metaplasia increased by aging and occupied about 70% of all lesions. The intestinal metaplasia usually showed higher grade in the cases with multiple carcinomas than those with single lesion. It should be considered that intestinal metaplasia plays the important role in the histogenesis of gastric carcinoma, especially in that of the well-differentiated one.     

A HISTOPATHOLOGICAL STUDY OF THE HUMAN SPINAL GANGLIA. NORMAL VARIATIONS IN AGING

The cervical and lumbar ganglia from 285 cases of a consecutive series of autopsies were examined to obtain quantitative data on the number and size of nerve cells, the ratio of connective tissue to parenchym and the appearance ratio of degenerated cells. Twelve cases without referable diseases in the central and peripheral nervous systems were selected as standard material representing normal aging processes. Serial sections were observed in order to avoid misjudgement. The average number of nerve cells varied from 7.0 × 10⁴ in the first decade to 4.5 × 10⁴ in the seventh decade, showing a decrease of about 2.5 × 10⁴ cells (36%). The average size of nerve cells remained fairly constant after the second decade, ranging between 25μ and 135mU with a single peak at 60 μ in a nearly standard curve distribution. Degenerated cells first appeared in the late second decade and there was an increase of connective tissue after the fourth decade. The age-dependent changes in the ganglia started in the third decade as cellular atrophy and satellitosls. Gradual replacement of the nerve elements by fibrous connective tissue took place during the following several years by the form of selerotic change and residual nodule. These age-dependent qualitative and quantitative changes should be important in the interpretation of findings in cases with delicate sensory disturbances.     

A HISTOPATHOLOGICAL STUDY ON THE EFFECT OF VARIOUS DRUGS IN EXPERIMENTAL MURINE LEPROSY

The effect of a high molecular unsaturated fatty acid C₂₂H₃₆O₂ (RTB) In the treatment of experimental murine leprosy was studied for a one-year long term in parallel with Isonicotinic acid hydrazide (INAH), its derivatives Isonicotinoyl 3–4-diethoxy benzol hydrazone (No. 284), and a salivary gland hormone (Parotin). The effectiveness of RTB on experimental murileproma was found to be weaker than INAH and No. 284, but almost the same as Parotin. The healing mechanism of RTB and Parotin is not a direct bacteriocidal action, but has a phagocytic and bacteriolytic mechanism of histiocytes due to activation of the reticuloendothelial system. The characteristic findings following RTB administration were predominant proliferation of "argyrophilic fiber type of histiocytes" and argyrophilic fibers, and the new formation of blood-capillaries. At the same time, the healing process of various drugs and the non-inhibitory effect of RTB were histopathologically discussed.     

HISTOPATHOLOGICAL AND MORPHOMETRICAL STUDY OF THE HUMAN EPIDIDYMIS AND TESTIS

Histopathological and morphometrical observations of the epididymis and testis were performed on 159 autopsy cases and 3 surgical materials of orchiectomy. The serum values of testosterone, FSH, LH, and values of intratesticular testosterone were measured in a certain number of the cases. Concerning the epididymis, increasing arteriosclerotic change was observed in the older epididymis. A high incidence of hyalinization of small arteries and arterioles was found in the younger epididymis. Eosinophilic microbodies were seen in the epithelial nuclei of the ductus epididymidis with high incidence in the older age groups. They were thought to be a catabolic compound containing phospholipids and protein resulting from cell degeneration of the mucosal epithelium. The diameter of the ductus epididymidis decreased with age. Inflammatory changes and ensued periductal sclerosis were found in the efferent duct area with an extremely high incidence. Serum values of testosterone correlated with both the age and morphological scoring calculated on the basis of either clusters or individual Leydig cells. Furthermore, testicular volume was inversely correlated with age. ACTA PATHOL. JPN. 34: 1327–1342, 1984.     

大鼠实验性胃溃疡自愈期间垂体远侧部ACTH细胞的免疫电镜研究

用成年雄性Ｗｉｓｔａｒ大鼠，分为溃疡组和对照组。用免疫电镜包埋后免疫金间接法村记ＡＣＴＨ细胞，研究胃溃疡自愈过程中垂体远侧部ＡＣＴＨ细胞的超微结构变化，结果显示，在实验性胃溃疡第４ｄ，一些ＡＣＴＨ细胞含少量很小的分泌颗粒，沿质膜分布，有较多的ＡＣＴＨ细胞胞质出现许多空泡。溃疡第６ｄ，ＡＣＴＨ细胞的高尔基复合体发达，其扁囊膨大部含中等电子密度的内容物，其内可见金颗粒标记。溃疡６－１０ｄ，较多的ＡＣＴ