阻断胃经线上K~ 通道对针刺足三里治疗大鼠实验性胃痛痛阈的影响

本文利用K~ 通道阻断剂TEA(四乙胺离子),以两种剂量分别注射于胃经穴位,观察阻断胃经线上K~ 通道对针刺镇痛效应的影响,现将结果报告如下。1 材料与方法 健康Wistar大鼠,雌雄不限,体重200～250g,将大鼠测过基础痛阈后,取20mMTEA,分6ul和8ul两种剂量,分别注射于以下部位:足三里、气冲、臀部非穴处。每次注射后立即用毫针针刺双侧足三里穴,捻转平补平泻20min、每5min测痛阈1次,并持续观测针后30min的病阈变化。     

手十二井穴刺络放血对实验性脑缺血大鼠缺血组织K^＋,Na^＋浓度影响…

本文阐述了用Ｋ＾＋、Ｎａ＾＋敏感性微电极监测凝结大脑中动脉的大鼠脑缺血模型大脑皮层缺血区组织Ｋ＾＋、Ｎａ＾＋浓度的变化及手“十二井”穴刺络放血对其影响的动物实验，说明了手“十二井”穴刺络放血对缺血性中风发病机理中重要生化改变的Ｋ＾＋、Ｎａ＾＋浓度有良性调整作用，可能缓解了脑水肿的发生与发展。     

电针镇痛对大鼠痛阈指标的影响

将中医学针灸疗法和电物理技术有机地结合起来,形成了独具特色的电针疗法.它是将针灸针刺入穴位后,与电刺激器的输出端相连,通过改变电刺激的频率、波宽、强度、脉冲间隔等参数,产生酸、麻、胀、重等"得气"感,从而代替捻、转、提、插等手工行针,达到 "气至而有效"的效果.电针方法的采用,使针刺疗法的刺激量可以定量描述,大大促进了针刺疗法在国内外的推广和应用.我国自上世纪五、六十年代即开展了针刺镇痛与针刺麻醉的研究,积累了丰富的资料.1997年11月,美国国立卫生研究院(NIH)主持召开了一个关于针刺镇痛和针灸治疗阿片类药物成瘾的听证会,我国科学家作了大会发言,说明针刺镇痛已经引起西方国家的重视.我们就电针对大鼠痛阈的影响做了相关的研究.     

远近部位穴位对脑缺血大鼠脑组织Ca^2＋、Na^＋、K^＋含量影响的比较

目的 比较不同部位穴位对实验性脑缺血疗效的影响。方法 采用大鼠大脑中动脉暂时性缺血模型,以脑组织Ca^2＋、Na^ 、K^ 含量为指标,比较电针近脑部的百会、人中穴和远脑部的环跳、昆仑穴对脑缺血的影响。结果 两组穴位对脑组织中异常升高的Ca^2 、Na^ 含量均有不同程度的降低,但两组之间无差别。结论 头部穴位组和肢体部穴位组均对脑缺血／再灌注损伤具有一定的保护作用,两组之间比较未见显著差别。     

针刺“足三里”穴对实验性胃痛大鼠胃经线上K+浓度的影响

我们以前的工作发现,造成大风实验性胃痛时,胃经“足三里”穴处的K⁺浓度升高,针刺“上巨虚”时,“足三里”穴处的K⁺浓度上升幅度明显减小,提示经脉线上K⁺可能参与了针刺镇痛过程.为进一步考察针刺治疗内脏痛过程中外周相应经脉线上的K⁺变化特征,我们以胃痛为内脏病模型,以“足三里”为针刺穴位,动态监测了胃痛过程和针刺镇痛过程中胃经线上其它部位的K⁺浓度变化,现报告如下.     

Concentration Dependent Effect of (−)‐Epicatechin on Na+/K+‐ATPase and Ca2+‐ATPase Inhibition Induced by Free Radicals in Hypertensive Patients: Comparison with L‐ascorbic Acid

Although the antioxidant properties of flavonoids are well documented, it is still unclear whether these effects are dependent on radical scavenging or iron chelating activities. Oxidative stress, a state of excessive reactive oxygen species (ROS) activity, is associated with vascular disease conditions such as hypertension. Both the anti‐ and pro‐oxidant effects of tea catechins have been implicated in the alterations of cellular functions that determine their chemoprotective and therapeutic potentials in health and diseases. The present study examined the concentration dependent (10^?7 to 10^?4 m ) effects of (?)‐epicatechin and L‐ascorbic acid on Na⁺/K⁺‐ATPase and Ca²⁺‐ATPase activity in hypertensive patients and normal subjects. L‐ascorbic acid has been used as a positive control to compare the effect of (?)‐epicatechin. A significant (p < 0.0001) decrease in the activities of Na⁺/K⁺‐ATPase and Ca²⁺‐ATPase was observed in hypertensive patients compared with normal subjects. We report that (?)‐epicatechin shows a significant (p < 0.001) dose‐dependent protective effect against oxidative stress induced by tert‐butyl hydroperoxide (t‐BHP), which is manisfested as a decrease in the activity of erythrocyte Na⁺/K⁺‐ATPase and Ca²⁺‐ATPase, in hypertensive patients as well as normal subjects. The effect of L‐ascorbic acid was also significant (p < 0.001) and was comparable with that of (?)‐epicatechin. Copyright © 2012 John Wiley & Sons, Ltd.     

压灸百会穴配合针刺夹脊穴治疗颈性眩晕疗效观察

目的观察压灸百会穴配合针刺颈部夹脊穴治疗颈性眩晕的临床疗效。方法将88例患者随机分为两组,治疗组采用压灸百会穴配合针刺颈部夹脊穴治疗,对照组采用传统针刺法治疗,观察两组疗效。结果治疗组愈显率为66.7%,对照组愈显率为42.5%,两组比较差异具有统计学意义（P〈0.05）。结论压灸百会穴配合针刺颈部夹脊穴治疗颈性眩晕愈显率明显优于传统针刺法。