胃幽门螺杆菌感染患者血清抗体间接免疫荧光检查方法及其…

研究幽门螺杆菌（Ｈｐ）感染的一种无创伤性血清免疫荧光抗体检查技术，作为消化性溃疡（ＰＵ），慢性胃炎（ＣＧ）以及非溃疡性消化不良（ＮＵＤ）患者Ｈｐ感染的快速诊断和Ｈｐ清除与否的指标，并和内镜活检尿素酶法，直接细菌染色法比较，以确定ＨＰ血清免疫荧光的诊断价值。免疫荧光法测试结果，在前两法阳性组的阳性率为９６％，阴性组的阳性率为２２％，阴性健康组的阳性率为３％。Ｈｐ血清免疫荧光与非免疫荧光比较，前者对Ｈ     

30NCU／ml抗—HBc酶免疫法试剂盒的研制

本文研制了能直接检测原倍血清的３０ＮＣＵ／抗－ＨＢｃ试剂盒，结果判定仍以抑制率≥５０％为阳性。３０ＮＣＵ／ｍｌ试剂盒特异性１００％，精密性批内ＣＶ１１％，批间ＣＶ１２．３％；检测ＨＢＶ血清盘阳性符合率９６．２％，阴性符合率１００％；稳定性：４℃６个月，３７℃３周内仍稳定；与本室１ＮＣＵ／ｍｌ试剂盒对比符合率９８．１％，与市售１ＮＣＵ／ｍｌ试剂盒比符合率９６．３％。     

血清α－L－岩藻糖苷酶及糖类抗原CA－50对原发性肝细胞癌诊断的探讨

对７８例原发性肝细胞癌（ＨＣＣ）、１５例其他消化道恶性肿瘤，１９例肝硬化、５例肝血管用及健康对照３０例，同时检测α－Ｌ－岩藻糖苷酶（ＡＦＵ）、糖类抗原ＣＡ－５０并与甲胎蛋白（ＡＦＰ）对照。结果：ＡＦＵ、ＣＡ－５０对ＨＣＣ诊断的阳性率分别为７６．２％和６９．２％高于ＡＦＰ（６２．８％）。２７例ＡＦＰ阴性的ＨＣＣ中，ＡＦＵ、ＣＡ－５０呈阳性者分别为１７和９例。三者联合检测诊断的特异性为８５．２％，敏感性达９１．１％。１６例术后检测结果，ＡＦＵ阴转率为８７．５％，ＣＡ－５０为８１．３％。ＡＦＵ和ＣＡ－５０做为诊断ＨＣＣ的标志物，对ＡＦＰ阴性者有互补诊断价值，联合检测诊断率更高。     

幽门螺杆菌相关胃十二指肠疾病患者血清CagAHpIgG的检测及临床意义

目的：为探讨细胞毒素相关蛋白（ＣａｇＡ）与幽门螺杆菌（Ｈｐ）相关胃十二指肠疾病的关系。方法：应用间接ＩＬＩＳＡ法定性检测１７０例胃镜受检者ＣａｇＡＨｐＩｇＧ抗体。结果：１７０例受检查中,Ｈｐ感染者１０６例,其中ＣａｇＡ阳性６９例,阳性率为６５．０９％,Ｈｐ感染的ＤＵ、ＧＵ、ＧＣａ患者ＣａｇＡ阳性率明显高于Ｈｐ感染的ＣＧ患者（Ｐ〈０．０５）,ＤＵ、ＧＵ、ＧＣａ组间无明显差别（Ｐ〉０．０５）。结论：提示ＣａｇＡ与Ｈｐ相关的严重胃十二指肠疾病密切相关。     

血清学与^13C—尿素呼吸试验检测反复腹痛儿童Hp感染比较

目的 探讨血清学及＾１３Ｃ尿素呼吸试验（＾１３Ｃ－ｕｒｅａ ｂｒｅａｔｈ ｔｅｓｔ,＾１３Ｃ－ＵＢＴ）在检测小儿反复腹痛（ｒｅｃｕｒｒｅｎｔ ａｂｄｏｍｉｎａｌ ｐａｉｎ,ＲＡＰ）幽门螺杆菌（Ｈｐ）感染中的应用及ＲＡＰ与Ｈｐ的关系。方法 采用＾１３Ｃ－ＵＢＴ及血清ＥＬＩＳＡ汉检测抗Ｈｐ－ＩｇＧ。结果 ２００例经＾１３Ｃ－ＵＢＴ检测有５８例检出Ｈｐ感染,检出率２９．４％（１５／５１）,２９．５％（３     

疣状胃炎与幽门螺杆菌感染关系的研究

目的：探讨疣状胃炎与幽门螺杆菌感染的关系。方法：内镜诊断的３０例疣诊断的３０例疣状胃炎与３０例慢性浅表性胃炎对照,均进行胃粘膜病理得到确诊,检测幽门螺杆菌（下称Ｈｐ）比较。结果：疣状胃炎组Ｈｐ阳性率为７６．７％（２３／３０）。对照组为３６．７％（１１／３０）,两组相比有显著性差异（Ｐ〈０．０１）。结论：Ｈｐ是疣状胃炎的重要病因之一。     

胆胰疾病的超声内镜与ERCP对比研究

胆胰疾病的超声内镜与ＥＲＣＰ对比研究［金震东，等．中国超声医学杂志，１９９４；１０（３）：１１］本文报告１００例胆胰疾病的ＥＲＣＰ与超声内镜（ＥＵＳ）对比研究，结果显示：ＥＲＣＰ诊断胆胰疾病的敏感性与准确性分别为８５％和８６％；ＥＵＳ分别为９６％和９...     

血清孕酮及hCG水平对异位妊娠保守治疗的影响

为探讨血清孕酮（Ｐ）及ｈＣＧ水平对异位妊娠药物治疗结果的影响，对４８例未破裂型异位妊娠药物治疗前血清Ｐ及ｈＣＧ水平进行了放射免疫测定，并以ｈＣＧ日增倍数（ｈＣＧ１／ＤＴ）反映ｈＣＧ增长率。结果：治疗成功组血清Ｐ水平及ｈＣＧ１／ＤＴ明显低于失败组。Ｐ≤３０ｎｍｏｌ／Ｌ者治疗成功率明显高于Ｐ〉３０ｎｍｏｌ／Ｌ者，以Ｐ≤３０ｎｍｏｌ／Ｌ预测治疗结果的敏感性及特异性分别为７３．１％及８１．８％，阳性及阴性     

性高危人群性病病原感染的病因学研究

本文报道用ＰＣＲ和培养法对１６９７例泌尿生殖道感染病人的标本进行检测Ｇｃ与Ｃｔ和分离培养Ｕｕ、Ｍｈ、Ｔｖ和Ｃｄ。结果显示：患Ｇｕ１０６例，Ｇｃ、Ｃｔ、Ｕｕ和Ｍｈ感染阳性率分别为９０．５６％、２４．５２％、４１．５０％和１６．０３％，男女无显著性差异（Ｐ＞０．０５）。患ＮＧＵ９４０例，Ｇｃ为５．３１％、Ｃｔ３９．６８％、Ｔｖ为３．７２％、Ｃｄ为１２．２３％，男女有非常显著性差异（Ｐ＜０．０１），Ｕｕ和Ｍｈ感染阳性率分别为５０．２１％和１９．０４％，男女无显著性差异（Ｐ＞０．０５）。Ｃｐ患者６５１例，Ｇｃ为１８（２．７６％）、Ｃｔ为１８７（２８．７３％）、Ｕｕ为１３９（２１．３５％）、Ｍｈ为５５（８．４５％）、Ｔｖ为１（０．１５％）、Ｃｄ为１８（２．７６％）。我们发现不同病种患者存在混合感染，Ｇｕ患者，其Ｇｃ＋Ｃｔ、Ｇｃ＋Ｕｕ混合感染阳性率比ＮＧＵ高，而ＮＧＵ与Ｃｐ患者，其Ｃｔ＋Ｕｕ＋Ｍｈ混合感染阳性率比Ｇｕ高。我们认为本研究结果可为临床泌尿生殖道感染病人提供临床诊断、治疗和预防依据。     

血糖快测法与胰岛素释放试验,C肽对糖尿病前期诊断的研究

探讨胰岛素释放试验（ＧＳＩＲＴ）、Ｃ肽（ＣＰ）对糖尿病前期（ＰＤＰ）的诊断价值，５０例Ⅱ型糖尿病（Ⅱ－ＤＭ）用血清胰岛素（ＩＲＩ）餐后３小时值／空腹值≥４，ＣＰ〉４μｇ／Ｌ及葡萄糖耐量试验进行验证，ＧＳＩＲＴ及ＣＰ对Ⅱ－ＤＭ诊断的敏感性和特异性分别为９６％及７６％。用ＧＳＩＲＴ及ＣＰ查出ＰＤＰ３５例中，符合葡萄糖耐量减低（ＩＧＴ）占２２．８５％。并随机抽查６０例Ⅱ－ＤＭ中的不同时期血糖快测情况，Ｆ     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.