荨麻疹患者血浆D二聚体、活化凝血因子Ⅶ及组织因子途径抑制物-活性凝血因子Ⅹ复合物与症状的关系

目的 探讨荨麻疹患者血浆D二聚体、活化凝血因子（FⅦa）、TFPI/Ⅹa水平与症状的关系。方法 酶联免疫吸附法检测急、慢性荨麻疹患者和健康献血者血浆D二聚体、FⅦa、TFPI/Ⅹa的水平，分析它们之间及其与症状评分、病程的关系。结果 急性荨麻疹患者的血浆D二聚体水平（450.57 ± 242.13） ng/mL高于正常人对照组（266.81 ± 40.68） ng/mL。血浆FⅦa水平（2.23 ± 0.74） ng/mL低于正常人对照组（5.23 ± 1.35） ng/mL，组间比较差异均有统计学意义（P < 0.01）。急性荨麻疹患者血浆TFPI/Ⅹa水平（0.87 ± 0.13） nmol/L与正常人对照组（0.88 ± 0.12） nmol/L比较，组间差异无统计学意义（P > 0.05）。慢性荨麻疹患者的血浆D二聚体水平（593.80 ± 294.04） ng/mL高于正常人对照组，组间比较差异有统计学意义（P < 0.01）；慢性荨麻疹患者血浆FⅦa水平（3.98 ± 0.35） ng/mL低于正常人对照组，组间比较差异有统计学意义（P < 0.01）。慢性荨麻疹患者血浆TFPI/Ⅹa水平（0.87 ± 0.16） nmol/L与正常人对照组比较，组间差异无统计学意义（P > 0.05）。急性荨麻疹患者D二聚体水平和FⅦa水平低于慢性荨麻疹患者，组间比较差异均有统计学意义（P < 0.05）。急、慢性荨麻疹患者血浆D二聚体与症状评分呈正相关关系（r = 0.68，P < 0.01；r = 0.82，P < 0.01），与病程无明显相关性（P > 0.05），FⅦa和TFPI/Ⅹa水平与症状评分关系及病程无明显相关性（P > 0.05）。结论 荨麻疹患者存在凝血系统激活和凝血因子消耗及继发性纤溶，提示D二聚体、FⅦa可能与荨麻疹症状有关。     

慢性荨麻疹患者血浆D-二聚体、凝血酶原片段F1＋2及凝血因子VⅡ水平的检测

目的：检测血浆D-二聚体、凝血酶原片段F1＋2及凝血因子VⅡ在慢性荨麻疹患者中的表达。方法：采用酶联免疫吸附法（ELISA）检测慢性荨麻疹患者血浆中的D-二聚体、凝血酶原片段F1＋2及凝血因子VⅡa的水平。结果：慢性荨麻疹患者血浆中D-二聚体、凝血酶原片段F1＋2及因子VⅡa的水平均明显高于正常对照组（P〈0．05和P〈0．01）。结论：凝血机制可能参入慢性荨麻疹的发病。     

慢性荨麻疹患者血清中Ｄ-二聚体水平和IL-33表达及意义

 目的:探讨慢性荨麻疹（CU）患者血清中D-二聚体水平和 IL-33的水平及其临床意义。方法:将63例CU患者依据自身血清皮肤试验（ASST）结果进行分组,取同期体检健康者作为对照组,采用ELISA法检测三组患者血清中D-二聚体水平和 IL-33水平并进行比较。结果:63例CU患者中,37例ASST阳性（58.73%）,26例阴性（41.27%）。相比对照组D-二聚体水平［2.437 ±0.120） mg/L］,ASST（+）组 ［（25.797±7.756） mg/L］和ASST（-）组［（30.605±9.101）mg/L］均明显增加（t值分别为10.25、10.58,P值均<0.05）,但ASST（-）组与ASST（+）组两组间无明显差异（t=1.39, P＞0.05）。ASST（+）组血清中IL-33含量 ［（0.237±0.037） pg/mL］明显高于对照组［（0.069±0.001） pg/mL］,t=7.78,P<0.05,而ASST（-）组IL-33水平［（0.112±0.028） pg/mL］无明显变化（t=2.63, P＞0.05）,ASST（-）和ASST（+）间有明显差异（t=4.69, P<0.05）。结论:CU患者血清中D-二聚体水平及ASST（+）组IL-33水平升高,可能导致机体免疫系统失衡,在CU的发生与发展中发挥一定作用。     

炎症因子及凝血因子与慢性荨麻疹发病及预后的相关性

目的：分析慢性荨麻疹患者血液学指标与发病及预后的相关性。方法：检测138例慢性荨麻疹患者及100例正常对照的血清总IgE、CRP、补体C3、补体C4、D2聚体、F1+2片段、FVIIa水平,并进行多因素Logist回归分析。结果：慢性荨麻疹患者血清中总IgE值、补体C3、C4、CRP、F1+2片段、FVIIa 及D2聚体与水平显著高于对照组,差异具有统计学意义( P＜0.05);87例慢性荨麻疹患者抗组胺药物治疗3个月后有效,其CRP、FVIIa、D2聚体、疾病严重程度评分明显高于治疗有效组,差异具有统计学意义( P＜0.05)。慢性荨麻疹抗组胺治疗无效组进行多因素Logist回归分析示D2聚体为治疗抵抗的主要影响因素（OR：1．63,95％CI 1.102-1.799,P<0.05）。以D2聚体的中位数（5.41 mg/L）分为两组,A组（≥5.41 mg/L）,B组（＜5.41 mg/L）,治疗后随访2年显示A组的复发率（23.56%）明显高于B组（12.14%）,差异具有统计学意义（P）＜0.001）。结论：多种炎症因子及凝血因子可能参与慢性荨麻疹的发病;D2聚体水平可能与慢性荨麻疹治疗的疗效及复发相关。     

凝血因子与荨麻疹的关系

荨麻疹是一种常见的、复发性的皮肤病.其发病机制复杂,至今尚未完全清楚.近年来有学者提出荨麻疹发病可能与凝血状态有关,并对凝血酶原片段F1+2、D二聚体、因子Ⅶ和因子Ⅻ等凝血因子进行相关研究,认为慢性荨麻疹患者体内存在外源性凝血级联反应激活以及纤溶状态,凝血酶生成可能在荨麻疹的发病中起着作用.抗凝治疗在荨麻疹药物治疗中显示出一定的临床应用前景.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

凝血因子与荨麻疹的关系

Urticaria is a common, recurrent and refractory skin disease. The exact pathogenic mechanism of urticaria is complex and unclear. It has been proposed that the development of urticaria is associated with coagulation status. Related studies on thrombinogen fragment F (1+2), D dimmer, factor Ⅶ and factor Ⅻ revealed the activation of extrinsic pathway of coagulation cascade and signs of fibrinolysis in patients with chronic urticaria. Thromhin generation may play a key role in the pathogenesis of urticaria. And anticoagulant drugs have exhibited a good prospect in the medication of urticaria.     

慢性荨麻疹患者血浆凝血酶原片段F1＋2水平的研究

目的：分析慢性荨麻疹与血浆F1＋2之间的关系。方法：用酶联免疫吸附双抗夹心法（ELISA），检测34例自体血浆皮肤试验（APST）阳性的慢性荨麻疹患者，治疗前后血浆凝血酶原片段F1＋2水平。结果：慢性荨麻疹经治疗后血浆凝血酶原片段F1＋2水平明显降低（P〈0．001），治疗前较正常对照组高（P〈0．001），治疗后与正常对照组的水平差异无显著性（P〉0．05）。结论：慢性荨麻疹血浆F1＋2水平升高，提示其可能在慢性荨麻疹发病中具有重要意义     

淋巴细胞、炎性因子及D-二聚体检测在慢性荨麻疹诊断中的价值分析

目的探讨淋巴细胞、炎性因子及D-二聚体检测在慢性荨麻疹诊断中的应用价值。方法纳入2018年1月1日~2019年1月1日我院就诊的CU患者80例进行研究,作为观察组。选取同时期来我院进行健康体检的志愿者80例为对照组。采集研究者空腹静脉血5ml,及时送检。观察外周血T、B淋巴细胞水平、D-二聚体、TNF-α、IL-6水平。结果观察组外周血T、B淋巴细胞水平低于对照组,差异显著(P <0.05);观察组D-二聚体、TNF-α、IL-6水平高于对照组,差异显著(P <0.05)。结论淋巴细胞、炎性因子及D-二聚体检测在慢性荨麻疹患者中的应用价值较高,当其水平发生异常时,可辅助诊断患者的病情变化情况。     

慢性荨麻疹患者血浆RANTES、嗜酸粒细胞趋化因子、TNF-α与白三烯B4水平的测定

目的 探讨慢性荨麻疹患者血浆中调节激活正常T细胞表达和分泌的细胞因子(RANTES)、嗜酸粒细胞趋化因子(eotaxin)、TNF-α及白三烯B4(LTB4)的水平及其意义.方法 对41例慢性荨麻疹患者进行临床评价,按症状积分将病情分为轻型、中型、重型3级.应用咪唑斯汀10 mg每日1次,连续治疗4周.采用ELISA法测定20例健康志愿者与41例患者治疗前后血浆中RANTES、eotaxin、TNF-α与LTB4的水平.结果 ①慢性荨麻疹组血浆中RANTES、eotaxin、TNF-α和LTB4水平分别为(52.5 ±10.2)μg/L、(58.4±16.1)μg/L、(35.1±9.6)ng/L和(109.4±21.7)ng/L,健康对照组水平分别为(33.7±9.4)μg/L、(48.3±13.6)μg/L、(21.3±8.9)ng/L和(77.8±11.6)ng/L,两组比较差异均有统计学意义,P值分别<0.01、<0.05、<0.01、<0.01.②血浆RANTES、eomxin、TNF-α、LTB4水平与临床表现的相关性分析显示:中、重型慢性荨麻疹患者的RANTES、eotaxin、TNF-α与LTB4血浆水平高于轻型患者,差别均有统计学意义,P<0.05;重型患者的RANTES、eotaxin、TNF-α与LTB4血浆水平较中型患者略有增高,但差异均无统计学意义,P>0.05.③咪唑斯汀治疗后慢性荨麻疹组血浆中RANTES、eotaxin、TNF-α和LTB4水平较前明显下降,分别为(36.3±8.9)μg/L、(46.3±10.2)μg/L、(23.2±7.5)ng/L和(83.1±14.2)ng/L,与治疗前比较差异有统计学意义,P值均<0.01,与健康对照组比较P值均>0.05.结论 慢性荨麻疹患者血浆RANTES、eotaxin、TNF-α吨与LTB4水平升高,并与病情的严重程度呈正相关趋势,咪唑斯汀治疗后血浆中RANTES、eotaxin、TNF-α和LTB4水平较前明显下降,提示RANTES、eotaxin、TNF-α与LTB4在慢性荨麻疹的发病过程中可能起一定作用.     

慢性荨麻疹患者血清ECP水平的测定

用法玛西亚CAPECP荧光酶标法测定30例慢性荨麻疹患者和20名正常人血清嗜酸细胞阳离子蛋白(ECP)水平。结果 ,慢性荨麻疹患者血清ECP值为25.76±2.45μg/L,正常对照组为3.3±0.74μg/L。患者组明显高于正常组(P<0.01)。因此 ,在荨麻疹的发病过程中 ,ECP可能起着一定的作用。     

女性慢性荨麻疹患者血清性激素水平的检测

荨麻疹是一种常见皮肤病,其病因及发病机制较复杂,尤其是慢性荨麻疹,不少学者在多方面对其进行了研究,其中血清性激素的变化与发病的关系,国内报道较少。我们采用放射免疫法,对女性慢性荨麻疹患者血清中睾酮、黄体生成素等6种性激素水平进行测定,以探讨性激素变化在慢性荨麻疹发病机理中的意义。     

急性荨麻疹和慢性荨麻疹患者血浆成分吸附组胺能力的测定

采用高效液相层析技术以快速、简便地枪测人体血浆组胺(HA)含量及血浆成份吸附HA的能力.结果显示正常人血浆HA含盈为0.07-0.18ng/ml(n=22).单位量丙种球蛋白HA吸附率显著大于白蛋白,单位量血浆中白蛋白吸附HA总量显著大于丙种球蛋白的吸附摄.正常人每毫升血桨HA吸附率约为93.68±5.43%.急性尊麻疹及慢性尊麻疹发作期血浆HA水平明显升高(P<0.01),血浆HA吸附率明显下降(P<0.01)0急性尊麻疹皮损消退时,血浆HA水平略高于正常,血浆HA吸附率恢复正常,而慢性尊麻疹皮损消退后血浆HA水平及血浆HA吸附串皆恢复正常.