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The antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a systematic of relatively rare autoimmune diseases with unknown cause. Kidney involvement is one of the most common clinical manifestations, and the degree of renal damage is closely associated with the development and prognosis of AAV. In this study, we utilized the Robust Rank Aggreg (RRA) method in R to integrate , GSE104948, GSE104954, GSE108109, and GSE108112 profile datasets loaded from Gene Expression Omnibus (GEO) database and identified a set of differentially expressed genes (DEGs) in kidney between AAV patients and living donors. Then, the results of gene ontology (GO) functional annotation showed that immunity and metabolism involved process of AAV both in glomerulus and tubulointerstitial. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that following pathways, such as complement and coagulation cascades pathway; Staphylococcus aureus infection; disease-COVID-19; and systemic lupus erythematosus (SLE) pathway play a crucial role in AAV. Next, the results analyzed by protein–protein interaction (PPI) network and Cytoscape software exhibited the hub genes ALB, TYROBP, and CYBB existed in both glomerular and tubulointerstitial compartments datasets. Finally, KEGG analysis using genes of two most important modules also further validated complement and coagulation cascades pathway and S. aureus infection existed both in glomerulus and tubulointerstitial compartments datasets. In conclusion, this study identified key genes and pathways involved in kidney of AAV, which was benefit to further uncover the mechanisms underlying the development and progress of AAV, biomarkers, and potential therapeutic targets as well. GSE108113相似文献
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目的探讨RNA干扰树突细胞(Dc)组织相容性复合物1(MHC-1)表达后获得的CD8+CD28-抑制性T细胞(Ts)对小肠移植免疫耐受的的影响。方法通过siRNA干扰DC MHC—I表达后,诱导获得CD8^+CD28^-Ts。建立由Wistar大鼠移植到SD大鼠的小肠移植模型36例,随机分为A组(转染实验组)、B组(未转染组,注射普通T细胞)和C组(移植对照组,注射生理盐水)。术后14d,每组各随机挑选6例,取移植大鼠小肠和血液标本行移植小肠组织病理学检查.并检测移植大鼠血清TGF-β、IFN-γ水平及回肠黏膜Na^+-K^+-ATP酶活性,观察移植大鼠的存活时间。结果术后第14天,A组移植大鼠血清中TGF—β和IFN-γ表达水平高于B、C组(P〈0.05)。A组大鼠肠黏膜Na^+-K^+-ATP酶活性为(6.3±1.0)kU/g,明显高于B组的(3.6±0.9)kU/g和C组的(2.9±1.3)kU/g(P〈0.05)。A组移植小肠病理Parks评分分级明显低于B组和C组(P〈0.05)。A、B和C组移植后大鼠中位生存时间分别为32.0、17.5和21.0d,A组存活时间明显优于B组和C组(P〈0.05)。结论将RNA干扰DCMHC—I表达所获得的CD8^+CD28^-Ts过继小肠移植大鼠.可以减轻移植大鼠的损伤程度.抑制免疫排斥反应. 相似文献
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Estela Gimnez Ignacio Torres Eliseo Albert Jos‐Luis Piana Juan‐Carlos Hernndez‐Boluda Carlos Solano David Navarro 《American journal of transplantation》2019,19(9):2479-2494
Controversy surrounds the potential association between cytomegalovirus (CMV) infection and increased risk of mortality after allogeneic hematopoietic stem cell transplantation (Allo‐HSCT). A systematic literature search was conducted using the PubMed, EMBASE, and Web of Science databases, assessing the association between CMV infection, as documented by the pp65 antigenemia assay or by polymerase chain reaction (PCR) using blood specimens, and overall mortality (OM) and nonrelapse mortality (NRM) in the allo‐HSCT setting. Pooled effects were estimated using the generic inverse variance random effects model. Heterogeneity was evaluated by Cochrane's Q test and I2 statistics. The source of heterogeneity was investigated by meta‐regression and subgroup analyses. Twenty‐six of 1367 studies fulfilled eligibility criteria. CMV infection identified by PCR monitoring was significantly associated with an increased risk of OM and NRM (hazard ratio 1.47, 95% confidence interval [1.20‐1.81], P ≤ .001; hazard ratio 1.68, 95% confidence interval [1.14‐2.49], P = .05, respectively). In this setting, the use of preemptive antiviral therapy (PET) resulted in a twofold increased risk of OM and NRM. The estimated effect sizes were associated with allo‐HSCT modalities. Although our analyses point to an association between CMV infection and an increased risk of OM and NRM in allo‐HSCT recipients, the high heterogeneity across studies prevented drawing of robust conclusions on this matter. 相似文献