Urinary tract infection in the neurogenic bladder

There is a high incidence of urinary tract infection (UTI) in patients with neurogenic lower urinary tract function. This results in significant morbidity and health care utilization. Multiple well-established risk factors unique to a neurogenic bladder (NB) exist while others require ongoing investigation. It is important for care providers to have a good understanding of the different structural, physiological, immunological and catheter-related risk factors so that they may be modified when possible. Diagnosis remains complicated. Appropriate specimen collection is of paramount importance and a UTI cannot be diagnosed based on urinalysis or clinical presentation alone. A culture result with a bacterial concentration of ≥10³ CFU/mL in combination with symptoms represents an acceptable definition for UTI diagnosis in NB patients. Cystoscopy, ultrasound and urodynamics should be utilized for the evaluation of recurrent infections in NB patients. An acute, symptomatic UTI should be treated with antibiotics for 5–14 days depending on the severity of the presentation. Antibiotic selection should be based on local and patient-based resistance patterns and the spectrum should be as narrow as possible if there are no concerns regarding urosepsis. Asymptomatic bacteriuria (AB) should not be treated because of rising resistance patterns and lack of clinical efficacy. The most important preventative measures include closed catheter drainage in patients with an indwelling catheter and the use of clean intermittent catheterization (CIC) over other methods of bladder management if possible. The use of hydrophilic or impregnated catheters is not recommended. Intravesical Botox, bacterial interference and sacral neuromodulation show significant promise for the prevention of UTIs in higher risk NB patients and future, multi-center, randomized controlled trials are required.     

BMSCs在糖尿病胰腺微环境下分化的可行性研究

目的 BMSCs在体外分化为胰岛素分泌细胞存在分化效率低、成熟度差的问题。研究BMSCs在糖尿病猪胰腺微环境下分化为胰岛素分泌细胞的可行性。方法取1只4周龄雄性贵州小香猪骨髓,采用贴壁法制备BMSCs。15只8～10周龄雌性贵州小香猪,体重8～10 kg,随机分为正常组(A组)、糖尿病组(B组)和BMSCs移植组(C组),每组5只。B、C组连续3 d从耳缘静脉推注链脲菌素加四氧嘧啶溶液,连续2 d血糖>17 mmol/L提示糖尿病造模成功。C组将标记增强型绿色荧光蛋白(enhanced green?uorescent portein,EGFP)的第3代BMSCs(细胞密度为5×107个/mL)1.1 mL多点注射移植至胰腺包膜下,A、B组注射等量生理盐水。监测血糖至30 d后,取胰腺组织行HE染色观察并检测胰岛数目及直径;免疫荧光组织化学染色检测新生胰岛的胰岛素表达;激光捕获显微切割技术获得EGFP+细胞,提取总RNA,采用RT-PCR检测胰岛素mRNA和胰腺十二指肠同源框蛋白1(pancreatic and duodenal homeobox factor 1,PDX1)mRNA的表达;荧光原位杂交(fluorescence in situ hybridization,FISH)检测胰岛素基因和SRY(sexdetermining region ofthe Y chromosome)基因的共表达。结果移植18 d后C组血糖开始较B组明显降低,且随时间延长逐渐下降(P<0.05)。组织学观察发现BMSCs移植30 d后C组胰岛数目(10.9±2.2)个较B组(4.6±1.4)个明显增加,差异有统计学意义(P<0.05),与A组(12.6±2.6)个比较差异无统计学意义(P>0.05);C组新生胰岛直径(47.2±19.6)μm,明显小于A组(119.6±27.7)μm,差异有统计学意义(P<0.05),B组未见新生胰岛。免疫荧光组织化学染色显示C组新生胰岛有胰岛素表达。RT-PCR检测示C组EGFP+细胞有胰岛素mRNA和PDX1 mRNA表达。FISH检测C组细胞中有SRY基因和胰岛素基因共表达。结论 BMSCs在糖尿病猪胰腺微环境条件下可分化为胰岛素分泌细胞。     

Medical management of neurogenic bladder with oral therapy

This is a review of the most current literature on medical management of the neurogenic bladder (NGB) to treat detrusor overactivity (DO), improve bladder compliance and treat urinary incontinence. The use of antimuscarinics, alpha blockers, tricyclic antidepressants, desmopressin and mirabegron will be discussed along with combination therapy to improve efficacy. These medical therapies will be the focus of this review with surgical therapy and botulinum toxin injections being the subject of other articles in this series.     

Expression profiles of long non-coding RNAs in neurogenic bladder of spinal cord injured rats: a transcriptomic analysis

BackgroundGrowing evidence has indicated that long non-coding RNAs (lncRNAs) are important regulators of pathological and physiological processes through various mechanisms. However, the signature of lncRNA expression and the possible roles of lncRNAs in spinal cord injury (SCI) rat neurogenic bladder (NB) have not been comprehensively explored. In this study, the expression profiles of lncRNAs and mRNAs were explored in the bladder tissue of SCI rats using next-generation sequencing (NGS).MethodsTwenty female Wistar rats were randomly divided into SCI 1–3 and normal control (NC) groups. The spinal cord was completely transected at the T9–T10 level to establish the SCI model. Bladder tissues were collected on days 7, 14, and 28 after the operation. The expression profiles of lncRNAs were detected by NGS. Differentially expressed lncRNAs (DELs) were chosen for qRT-PCR verification to validate the RNA sequencing results. The functions of the predicted target genes were then evaluated using Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.ResultsCompared with the NC group, the SCI 1–3 groups had 468, 117, and 408 DELs [fold change (FC) >2], including 247, 38, and 201 up-regulated and 163, 79, and 207 down-regulated lncRNAs, respectively. Likewise, 6,654, 2,133, and 5,706 mRNAs (FC >2) were differentially expressed between SCI 1–3 and NC rats, of which 4,821, 1,195, and 3,695 were up-regulated, and 1,833, 938, and 2,011 were down-regulated, respectively. Specifically, Miat, Mir155hg, and H19 were significant DELs in all SCI groups. Moreover, GO revealed that the DELs were related to several terms, including immune response, and KEGG was mainly enriched in 10 pathways, such as the transforming growth factor β signaling pathway.ConclusionsThe results revealed the expression profiles and possible roles of lncRNAs in SCI rat NB. This study may help identify possible NB mechanisms following SCI from the perspective of lncRNAs and provides new potential lncRNAs for the early diagnosis and treatment of human NB in the future.     

脐带间充质干细胞调节炎性微环境干预急性肾损伤的效应研究

目的：观察移植外源性脐带间充质干细胞（UC-MSCs）对小鼠急性肾损伤（AKI）炎性微环境的影响及对肾组织的修复作用。方法：分离培养孕后期C57BL/6雌性小鼠的UC-MSCs。另取雌性C57BL/6小鼠60只,随机分为正常对照组、AKI组、AKI＋MSC移植组,每组20只。AKI组和AKI＋MSC组用微型动脉夹夹闭小鼠双侧肾蒂45 min复制AKI模型,并于模型制备成功时腹腔内注射生理盐水0.2 ml（AKI组）或1＆#215;106 UC-MSCs（AKI＋MSC组）。于第2天和第7天每组活杀10只小鼠,取眼球血及肾组织,检测血尿素氮（BUN）及血肌酐（SCr）水平;肾组织行HE染色,观察组织病理学变化;ELISA法检测肾组织匀浆中促炎因子IL-1β、TNF-α、干扰素-1（IFN-1）及抗炎因子碱性成纤维细胞生长因子（bFGF）、IL-10、B淋巴细胞瘤-2（Bcl-2）的水平。结果：2 d时AKI组小鼠肾小管上皮细胞肿胀,胞质空泡性变,BUN和Cr均显著高于正常对照组,提示造模成功后肾小管受损严重,7 d稍有减轻;AKI＋MSC移植组小鼠肾功能在2 d时较AKI组有所恢复,肾组织病理学变化明显减轻（P＜0.01）,7 d基本恢复正常。ELISA结果显示,与正常对照组比较, AKI组各时间点肾组织匀浆中促炎因子的水平均显著升高（P＜0.01或P＜0.05）,抗炎因子的水平均显著降低（P＜0.01或P＜0.05）,2 d较7 d更加明显。各时间点AKI＋MSC组抗炎因子的水平较AKI组明显升高,促炎因子的水平明显下降,但与正常对照组比较差异均有显著性（P＜0.01或P＜0.05）,7d较2 d改善更明显。结论：MSC可通过减轻AKI肾组织炎症反应,调节损伤肾脏组织微环境中的细胞因子水平发挥保护肾损害的修复作用。     

Urodynamic and physiologic patterns associated with the common causes of neurogenic bladder in adults

The clinical presentation of the neurogenic bladder can be as vast as the pathologic causes however urodynamics (UDS) can help guide clinical decision-making and help simplify a complex disease state. UDS may be considered as the gold standard in helping to break down complex and multifactorial voiding dysfunction into manageable goals; these include protecting the upper tracts, limiting urinary tract infections (UTI) via avoiding urinary stasis, and maintaining quality of life. Included within are examples of normal to pathologic tracings including normal filling and voiding, detrusor sphincteric coordination, changes in compliance, etc. Additionally we have provided expected UDS findings based on neurogenic disease process, including but not limited to, Parkinson’s, dementia, multiple sclerosis (MS) and spinal cord injury based on lesion location. Pattern recognition and understanding of UDS can help lead to quality of life improvements and optimal management for the patient with neurogenic bladder dysfunction.     

人脐带间充质干细胞移植对大鼠流产模型妊娠结局的影响

目的探讨人脐带间充质干细胞(WJ-MSCs)移植对大鼠流产模型妊娠结局的影响及机制。方法将48只孕鼠随机分为6组,每组8只。生理盐水对照组(a):孕6~8d皮下注射生理盐水0.4mg·kg~(-1)·d~(-1),孕9d尾静脉注射生理盐水1ml/kg;乙醇对照组(b):孕6~8d皮下注射75%乙醇0.4mg·kg~(-1)·d~(-1),孕9d尾静脉注射生理盐水1ml/kg;流产模型组(c):孕6~8d皮下注射溴隐亭0.4mg·kg~(-1)·d~(-1),孕9d尾静脉注射生理盐水1ml/kg;实验组d1、d2、d3分别于孕6~8d皮下注射溴隐亭0.4mg·kg~(-1)·d~(-1),孕9d尾静脉注射WJ-MSCs 1×105个、1×106个、1×107个(生理盐水使用量为1ml/kg,将细胞制成混悬液后注射)。各组大鼠均于孕14d处死并取材,观察各组大鼠胚胎吸收率,RT-PCR检测蜕膜组织白介素-10(IL-10)、干扰素-γ(IFN-γ)和IL-17mRNA的相对表达量。ELISA检测各组外周血IL-10、IFN-γ和IL-17的含量。结果 c组和d1组的平均胚胎吸收数和平均胚胎吸收率均显著高于a、b、d2、d3组(P0.05)。c组、d1组与a、b、d2、d3组比较,IL-10mRNA表达显著降低(P0.05),IFN-γ和IL-17mRNA表达显著升高(P0.05)。c组、d1组与a、b、d2、d3组比较外周血IL-10含量显著降低(P0.05),IFN-γ和IL-17含量显著升高(P0.05)。c组与d1组间,a、b组与d2、d3组间的观察指标比较均无显著性差异(P0.05)。结论一定浓度的WJ-MSCs移植入大鼠流产模型体内,可以降低胚胎吸收率,改善大鼠流产模型的妊娠结局;其机制可能与WJ-MSCs上调IL-10、下调IFN-γ和IL-17的含量有关。     

骨髓间充质干细胞移植对去卵巢骨质疏松骨量影响的实验研究

目的 探索骨髓间充质干细胞（BMSCs）移植对去卵巢骨质疏松大鼠骨密度的影响。方法 雌性SD大鼠随机分为空白对照组（A组）、模型组（B组）、细胞治疗组（C组）、雷诺昔芬药物治疗组（D组）。通过去卵巢建模成功后,C组通过尾静脉移植BMSCs,D组口服雷诺昔芬抗骨质疏松药物。结果 与A组相比,B组腰椎和股骨骨密度均明显降低（P＜0.01）。MSCs治疗后,C 组中的骨密度得到明显改善,并与B组比较具有统计学差异（P＜0.01）,亦优于D组。结论 BMSCs可以有效改善去卵巢大鼠骨质,这为绝经后骨质疏松的治疗提供了一种新的方法,为进一步临床应用提供了实验支持。     

血小板裂解液对人脐带间充质干细胞体外成软骨分化的影响

目的探讨血小板裂解液(platelet lysate,PL)在体外定向诱导人脐带间充质干细胞(human umbilical cord derived mesenchymal stem cells,hUCMSCs)分化成软骨细胞中的作用。方法取健康产妇自愿捐赠脐带,采用胶原酶消化法分离hUCMSCs,体外培养扩增,流式细胞仪进行细胞表型鉴定。根据加入诱导培养基成分不同将实验分为以下3组:A组为H-DMEM培养基、10%FBS及10%PL,B组为H-DMEM培养基、10%FBS、10 ng/mL TGF-β1、1×10-7 mol/L地塞米松、50μg/mL维生素C及1%胰岛素铁硒传递蛋白(insulin-transferrin-selenium,ITS),C组为H-DMEM培养基、10%FBS、10 ng/mL TGF-β1、1×10-7 mol/L地塞米松、50μg/mL维生素C、1%ITS及10%PL。诱导培养2周,甲苯胺蓝染色检测各组软骨细胞基质的分泌,免疫荧光检测软骨特异性Ⅱ型胶原表达,半定量RT-PCR检测蛋白聚糖(Aggrecan)和Ⅱ型胶原表达。结果分离得到的hUCMSCs不表达造血细胞的表面标记CD45、CD34和HLA-DR,而表达黏附分子和MSCs表面标记CD44、CD105和CD146。甲苯胺蓝染色和Ⅱ型胶原免疫荧光染色示C组呈阳性,B组呈弱阳性,而A组均呈阴性。半定量RT-PCR检测示Aggrecan和Ⅱ型胶原在B、C组中均有表达,A组中未见表达;C组Aggrecan mRNA和Ⅱ型胶原mRNA表达明显高于B组,差异均有统计学意义(P<0.05)。结论单纯10%PL不能诱导hUCMSCs成软骨分化,但它可当作成软骨诱导培养基的辅助添加剂,对hUCMSCs成软骨分化有明显促进作用,为构建组织工程软骨提供了新的可利用条件。     

Effect of erythropoietin on the oriented chemotaxis of bone-marrow mesenchymal stem cells under acute kidney injury microenvironment

Objective To investigate the migration of bone-marrow mesenchymal stem cells (BMSCs) under acute kidney injury (AKI) microenvironment in vitro and the effect of erythropoietin (EPO) intervention, and to explore its underlying mechanism. Methods Renal tubular epithelial cells (RTECs) were cultured in hypoxia/ re-oxygenation (HR) condition for 12 h, respectively, in order to establish HR-RTEC. BMSCs and RTECs were co-cultured by Transwell system and were divided into 7 groups: control group (group①, only BMSC cultured), BMSC-RTEC co-culturing group (group②), BMSC-HR-RTEC co-culturing ＋EPO intervention groups (group③to group⑦, EPO concentration: 0, 1, 5, 10, 50 IU/ml). All the groups were cultured for 48 h and the number of migrating BMSCs was detected. Western blotting was applied for the detection of SDF-1 expression in RTECs and p-MAPK and MAPK levels in BMSCs. SDF-1 concentration in the RTECs culture supernatant was tested by ELISA. Results The number of BMSCs migrating to the low chamber where HR-RTECs were cultured was increased, and EPO intervention further enhanced this migration which reached the peak at the concentration of 10 IU/ml [Compared with group③, (46.67±7.37) cells vs (19.00±2.37) cells, P＜0.05]. Intracellular expression level and the secreated level of SDF-1 in HR-RTECs in group③ were higher than those in RTECs of group② [0.37±0.01 vs 0.19±0.01, P＜0.05; (61.64±4.88) μg/L vs (35.26±8.78) μg/L, P＜0.05]. EPO intervention increased above SDF-1 levels and reached the peak at the concentration of 10 IU/ml [group⑥ vs group③：(173.53±14.66) μg/L vs (61.64±4.88) μg/L, P＜0.05], accompanied with enhanced phosphorylation of MAPK in BMSCs. Conclusions AKI microenvironment has obvious chemotaxis effect on BMSCs, and EPO intervention can strengthen this effect. The increased SDF-1 level and enhanced phosphorylation of MAPK, the downstream signal protein of SDF-1/CXCR4 axis, are the possible mechanism for EPO performance.     

不同数量BMSCs对大鼠背根神经节生长影响的实验研究

目的 BMSCs作为雪旺细胞的替代细胞可提高周围神经损伤修复效果,但目前对于神经支架内添加适宜的种子细胞数量未达成共识。研究不同数量BMSCs对大鼠背根神经节(dorsal root ganglion,DRG)生长的影响。方法取4周龄雄性SD大鼠3只,体重80～100 g,体外培养扩增BMSCs。取新生1～2日龄SD大鼠3只,雌雄不限,体重4～6 g,制备DRG。取第3代BMSCs制备BMSCs-生物蛋白胶复合物,按照加入细胞数量的不同,将实验分为A组(1×103个)、B组(1×104个)、C组(1×105个)和D组(0个),并与SD大鼠DRG共培养。48 h后通过形态学观察,神经丝蛋白200和雪旺细胞S-100免疫荧光染色,对SD大鼠DRG轴突生长长度、雪旺细胞迁移距离和轴突面积指数进行定量评价。结果 48 h后可见各组BMSCs生长出多个长突起;雪旺细胞移行和轴突从DRG长出,呈多方向性生长;BMSCs生物蛋白胶具有生物活性且影响DRG生长。A、B、C组DRG轴突生长长度及雪旺细胞迁移距离均明显大于D组(P<0.05),C组小于B组(P<0.05),A、C组间及A、B组间差异无统计学意义(P>0.05)。A、B组轴突面积指数明显大于D组(P<0.05),C组大于D组但差异无统计学意义(P>0.05),A、B、C组间差异无统计学意义(P>0.05)。结论乳鼠DRG体外培养实验,可作为研究周围神经损伤修复的体外模型。不同数量BMSCs生物蛋白胶复合物对DRG生长的影响具有量效关系,为组织工程神经选用合适数量BMSCs提供了理论依据。     

Extracorporeal shock wave therapy combined with engineered mesenchymal stem cells expressing stromal cell-derived factor-1 can improve erectile dysfunction in streptozotocin-induced diabetic rats

BackgroundFor erectile dysfunction (ED) in diabetes mellitus (DM) patients who have poor response to drugs, extracorporeal shock wave therapy (ESWT) and engineered mesenchymal stem cell (MSC) therapy have been studied as alternative treatment options. The objective of this study is to investigate whether ESWT in combination with stromal cell-derived factor-1 expressing engineered mesenchymal stem cell (SDF-1 eMSC) therapy can have synergistic effects on ED in streptozotocin-induced diabetic rats.MethodsFifty 8-week-old male Sprague-Dawley rats were randomly divided into five groups (N=10 per group): (I) Normal group, (II) DM ED, (III) DM ED + ESWT group, (IV) DM ED + SDF-1 eMSC group, and (V) DM ED + ESWT + SDF-1 eMSC group. Each groups were treated with bilateral injections of SDF-1 eMSC or ESWT following the experiment protocol for eight weeks.ResultsThe ratio of ICP/MAP was distinctly higher in the DM ED + ESWT + SDF-1 eMSC group than that in the DM ED group. Concentration of α-smooth muscle actin (α-SMA) was elevated the highest in the DM ED + ESWT + SDF-1 eMSC group. Additionally, ESWT increased the intensity of SDF-1 expression in the corpus cavernosum. ESWT + SDF-1 eMSC treatment also induced neuronal nitric oxide synthase (nNOS) and NO/cGMP expression in the corpus cavernosum. Furthermore, numbers of penile progenitor cells were increased in DM ED rats.ConclusionsCombined treatment of ESWT with SDF-1 eMSC treatment is more effective than by a single therapy. It could be used as a potential and effective synergistic treatment for DM ED.     

胎盘源性干细胞在肝脏疾病中的研究进展

人类胎盘源性干细胞(hPDSCs)是干细胞的混合群.再生医学已将其用于某些功能衰竭和损伤器官的细胞再生、抗细胞凋亡、抗炎,抗肿瘤和细胞功能恢复研究.目前已有许多实验研究证明:胎盘间充质干细胞(PDMSCs)可以在体外分化为肝细胞样细胞,并于体内外促进干细胞增生和抗肝细胞凋亡,在动物肝损伤模型抑制肝纤维化.本文就胎盘干细胞的来源、分类、生物学特性以及胎盘干细胞在肝脏疾病中的治疗研究做一综述,以便为进一步探讨胎盘源性干细胞在肝脏疾病治疗中的应用提供新的思路.     

左、右归丸对去卵巢大鼠骨髓间充质干细胞成骨分化的影响

目的 基于AMPK/mTOR信号通路探讨左、右归丸对去卵巢大鼠骨髓间充质干细胞成骨分化的影响及作用机制.方法 将60只雌性大鼠随机分为六组,其中一组为空白对照(KB组);一组模拟手术过程,灌胃蒸馏水(SHAM组);其余四组进行卵巢切除术,分别灌胃蒸馏水(OVX组)、补佳乐(BJL组)、右归丸(YGW组)和左归丸(ZGW...     

肿瘤干细胞微生景的耐药性及相关靶向治疗

肿瘤干细胞是近年来在许多肿瘤组织中发现的一类特殊干细胞,具有自我更新和分化的能力,可通过不断分化为肿瘤细胞,从而形成肿瘤原发灶和转移灶.肿瘤干细胞对化疗和放疗具有很强的不敏感性,这一特性主要与其微生景中的各种分子及其相互调控有关.目前一些新型的治疗策略是针对肿瘤干细胞及其微环境所研发的新的靶向治疗方法,如抑制新生血管形成、阻止细胞发生上皮一间质转化等.深入研究肿瘤干细胞及其微生景的耐药性,可为肿瘤治疗提供新途径.     

低氧对脂肪间充质干细胞向雪旺细胞分化的影响

目的探讨低氧对脂肪间充质干细胞（ADMSCs）向雪旺细胞（SCs）分化能力的影响。方法分离培养SD大鼠ADMSCs并用流式细胞仪、茜素红染色、油红O染色鉴定。将成功分离的ADMSCs随机分为3组：常氧诱导组,在常氧条件下（5％CO2,21％O2,37℃）诱导;低氧处理＋常氧诱导组,低氧处理（5％CO2,0．5％O2,37℃）后在常氧条件下诱导;低氧诱导组,低氧条件下（5％CO2,0．5％O2,37℃）诱导。观察各组细胞形态,MTT法检测细胞增殖情况,免疫荧光染色和Westernblot检测SCs标志物GFAP和S-100的表达。结果细胞分离后,经流式细胞仪分析可见细胞表面CD44阳性、CD45阳性、CD90阳性,茜素红及油红O染色均为阳性。MTT法检测结果：低氧处理＋常氧诱导组A值为0．861±0．039,高于常氧诱导组0．837±0．017,差异具有统计学意义（P〈0．05）;低氧诱导组A值为0．931±0．041,均高于常氧诱导组和低氧处理＋常氧诱导组（P均〈0．05）。免疫荧光染色发现常氧诱导组和低氧处理＋常氧诱导组大量细胞GFAP和S-100表达阳性,低氧诱导组仅少量细胞S-100和GFAP表达阳性。Westernblot检测发现常氧诱导组S-100蛋白表达最高,低氧处理＋常氧诱导组GFAP蛋白表达最高,低氧诱导组S-100蛋白、GFAP蛋白表达均最低。结论低氧抑制ADMSCs向SCs的分化,低氧处理后的ADMSCs在常氧条件下仍可向SCs分化。     

骨髓间充质干细胞对大鼠慢性胰腺炎中星状细胞的影响

目的 观察骨髓间充质干细胞(BMSCs)对慢性胰腺炎(CP)中星状细胞(PSC)的活化、增殖和分化的影响.方法 将80只SD大鼠随机分为空白组(20只,注射无菌生理盐水)、模型组[20只,尾静脉注射二氯二丁基酯(DBTC)制作大鼠CP模型]、治疗组(20只,于CP模型制作成功后尾静脉注射体外培养的同种异体GFP-MSCs)、假治疗组(20只,于CP模型制作成功后尾静脉注射等量的无菌生理盐水).取大鼠胰腺检测PSC活化表达的Desmin、胶质纤维酸性蛋白(GFAP)、α-平滑肌肌动蛋白(α-SMA)水平,组织中Ⅰ、Ⅲ胶原含量及白细胞介素(IL)-10、肿瘤坏死因子(TNF)-α、转化生长因子(TGF) -β1表达水平.结果 治疗组PSC表达的Desmin、GFAP、α-SMA,组织中Ⅰ (0.135±0.030) ng/g、Ⅲ胶原含量(0.029±0.008) ng/g,TGF-β1 (0.020±0.006) μg/L浓度均低于假治疗组(P＜0.05),但IL-10 (603.799±89.374) g/ml,TNF-α(507.45±90.13) μg/L均高于假治疗组(P＜0.05),后者与模型组之间的差异无统计学意义(P＞0.05),空白组未见明显异常.结论 BMSCs可以通过对细胞因子的调控减少PSC的活化,抑制其增殖及分化,降低细胞外基质的沉积.     

骨髓间充质干细胞促进糖尿病大鼠骨折愈合的研究

目的检测促炎因子TNF-α、IL-6及抗炎因子IL-4、IL-10以及Toll样受体4在骨髓间充质干细胞促进糖尿病大鼠骨折愈合过程中的动态变化,探讨炎症失衡在此过程中的作用及可能机制。方法 7~8周龄SPF级雄性自发性2型糖尿病GK大鼠56只,喂养高脂饲料造模。造模成功后(随机血糖≥11.1 mmol/L)暴露大鼠左侧股骨,人为造成开放性骨折并给与克氏针复位内固定,将培养收集浓缩的骨髓间充质干细胞注射于骨折周围肌肉软组织,于实验后第1周、3周、6周、8周分别行X线摄片、下腔静脉取血测定炎症因子、骨折局部软组织检测TLR4表达。结果术后第1周、3周、6周、8周对照组TNF-a、IL-6、TLR-4表达均高于实验组(P0.05),而IL-4、IL-10则低于实验组(P0.05);两组动物在手术后第三周均可见骨折处骨痂生长,实验组更加明显,但对照组骨痂不均匀,骨密度较低。结论骨髓间充质干细胞局部移植可以降低GK糖尿病大鼠骨折后机体的炎症反应,促进骨折愈合。