Ѫ�������ø-1�Ͱ�ϸ������-6�����ܲ�������о�

目的通过观察分析中重度牙周炎患者血清中对氧磷酶-1（PON1）活性及白细胞介素-6（IL-6）在牙周基础治疗前后的变化,探讨PON1和IL-6与牙周病之间的相关性以及两者在牙周病病情变化中的作用。方法2012年5月至2013年2月在辽宁省人民医院体检中心体检的牙周健康人群30名（牙周健康组）及口腔科就诊的中重度牙周炎患者39例（牙周炎组）,对所有受试者进行基础治疗,检查治疗前后PON1、IL-6以及临床牙周检查指标。结果牙周健康组与牙周炎组在基础治疗前比较：牙周炎症指标[出血指数（BI）、牙周探诊深度（PD）]及血清IL-6差异均有统计学意义（P〈0．05）,血清中PON1活性差异无统计学意义（P〈0．05）。牙周健康组在牙周基础治疗前后各项指标差异均无统计学意义（P〉0．05）。牙周炎组在牙周基础治疗前后比较：牙周炎症指标BI、PD、血清PON1及IL-6与治疗前比较差异均有统计学意义（均P〈0．05）。结论牙周健康人群和中重度牙周炎患者血清PON1活性无差异;牙周基础治疗可改善中重度牙周病患者牙周健康状况,提高血清中PON1活性,降低IL-6水平;中重度牙周炎患者在牙周基础治疗前后血清中PON1活性和IL-6水平的变化存在负相关性。     

牙周炎症对冠心病患者CRP水平影响的初探

目的通过横向研究初步探讨牙周炎症对冠心病患者CRP水平的影响。方法受检者共60人,包括冠心病＋牙周炎组、单纯牙周炎组和健康对照组,每组20人。检测超敏C反应蛋白（hs—CRP）,牙周临床指标包括牙周探诊深度、附着丧失、出血指数、菌斑指数,并检测血脂水平（HDL、LDL、TG、CHO）和白细胞计数。采用ANOVA法分析以上指标在各组间的差别,Pearson法分析hs—CRP与牙周指标间的相关性。结果牙周炎＋冠心病组hs．CRP显著高于单纯牙周炎组和健康组（P〈0．05）,单纯牙周炎组的hs—CRP水平又显著高于健康组（P〈0．05）。牙周炎＋冠心病组牙周指标均高于单纯牙周炎组和健康组。受检者的血清hs—CRP水平与牙周指数显著相关（P〈0．05）。结论牙周感染和炎症可能是牙周炎＋冠心病患者hs—CRP水平升高的原因之一。     

侵袭性牙周炎龈沟液中白介素-8 的检测

目的：检测侵袭性牙周炎（AgP）龈沟液中白细胞介素（IL-8）的总量和浓度并探讨其与牙周临床指标的关系。方法：采用常规滤纸条法收集侵袭性牙周炎实验组患牙（T1）和健康牙（T2）各位点及正常对照组（C）各位点的龈沟液（GCF）样本,用ELISA法检测各样本中IL-8的总量和浓度。结果：3组受检牙龈沟液中IL-8的总量和浓度不同。侵袭性牙周炎患牙组GCF中IL-8总量高于健康牙位组（P〈0．05）及正常对照组;而3组中IL-8浓度差异也有显著性,侵袭性牙周炎患牙组GCF中IL-8的浓度高于健康牙位组（P〈0．05）和正常对照组;虽然GCF中IL-8浓度与牙周探诊深度（PD）、牙龈出血指数（BOP）、附着丧失（AL）无相关关系;但IL-8总量与以上牙周临床指标相关。结论：在侵袭性牙周炎患者龈沟液中IL-8是参与牙周炎症反应的重要调节因子。     

牙周炎和冠心病患者血清中白介素1β和肿瘤坏死因子α的表达分析

目的：通过比较伴有慢性牙周炎的冠心病患者和慢性牙周炎患者的牙周状况和血清中细胞因子的含量,探讨牙周炎和冠心病之间的关系。方法：选取24例经冠状动脉造影确诊并伴牙周炎的冠心病患者和同样数量的慢性牙周炎患者及健康志愿者,由同一名医师进行牙周检查,抽取入选者的血清采用双抗体夹心ABC-ELISA法检测白介素1β（IL-1-β）和肿瘤坏死因子α（TNF-α）的含量。结果：均衡了性别、吸烟等因素后,冠心病合并牙周炎组与慢性牙周炎组之间的牙周附着丧失程度存在显著性差异（P〈0．005）,两组之间IL-1和TNF也存在显著性差异（P〈0．01）。结论：IL-1β和TNF-α可能与冠心病和牙周炎病理机制密切相关,牙周炎和冠心病之间可能存在一定的关联。     

���ܻ������ƶ԰鷢���IJ������������׻���Ѫ��IL-8��Ѫ֬ˮƽ��Ӱ��

目的探讨牙周基础治疗对伴发冠心病的牙周炎患者血清白细胞介素-8（IL-8）及血脂水平的影响。方法选择2007年2月至2011年9月来中国医科大学口腔医学院牙周病科就诊的伴发冠心痛（稳定期）的慢性中、重度牙周炎患者40例。对其进行内科维持治疗及牙周基础治疗。分别在牙周基础治疗前及治疗2个月后对患者行牙周检查,检查指标包括探诊深度（PD）、临床附着丧失（CAL）和龈沟出血指数（SBI）,并采集静脉血,检测血清IL-8以及血脂水平。结果牙周基础治疗2个月后,患者各牙周检查指标（PD、CAL和Sin）均有好转（P〈0．01）;血清IL-8水平明显降低（P〈0．01）;血脂指标中总胆固醇（TC—C）、低密度脂蛋白（LDL—C）水平均降低（P〈0．01、P〈0．05）,高密度脂蛋白（HDL—C）升高（P〈0．05）,甘油三脂（TG—C）水平虽也下降,但差异并无统计学意义（P〉0．05）。结论牙周基础治疗可改善牙周的炎症状态,并对血清IL-8及血脂水平产生影响。     

浅析中药对慢性牙周炎的辅助治疗作用

目的：探讨中药治疗慢性牙周炎的临床疗效。方法：20例慢性牙周炎女性患者,随机分为2组,牙周基础治疗后,实验组给予补肾清火的中药治疗,对照组不服中药,3个月后复查各项牙周指数的变化。结果：2组患者服药前,菌斑指数（PLI）、牙周探诊深度（PPD）、附着水平测定（PAL）,无显著性差异（P〉0．05）,3个月后复查,2组各项牙周指数有显著性差异（P〈0．01）。结论：中药对慢性牙周炎症有明显的改善作用。     

代谢综合症患者牙周状况的临床研究

目的：分析代谢综合症患者牙周状况,探讨代谢综合症在牙周疾病发生、发展中的意义。方法：选择150例诊断明确的代谢综合症患者为研究组,150例健康人作为对照组。检查记录：菌斑指数（PLI）、探诊深度（PD）、临床附着丧失（CAL）、出血指数（BI）、失牙数并计算牙周位点率。测定龈沟液及血清中白细胞介素-6（IL-6）、C反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α）水平。结果：代谢综合症组患侵袭性牙周炎、慢性牙周炎高于对照组（P〈0．01）;代谢综合症组PD、CAL、重度百分比及失牙数除BI外均高于对照组（P〈0．01）。代谢综合症患者龈沟液中IL-6、TNF-α、CRP水平高于对照组（p〈0．01）,而血清中两组无差异。相关性分析表明：MS组患者血糖水平与PLI、CAL、BI、重度百分比呈正相关（P〈0．01）,腹型（腰围水平）与PD、CAL、PLI重度百分比呈正相关（P〈0．01）。HDL-C水平与PLI、CAL、BI、重度百分比及失牙数正相关。血糖及HDL-C水平与牙周病的发病率正相关。结论：MS患者牙周疾病的发病率明显高于正常人,其中血糖、HDL-C及肥胖程度是影响牙周状况的重要因素,这种变化可能是通过MS引发炎症失衡而发生的。     

牙周基础治疗对2型糖尿病伴牙周炎患者糖代谢及血清TNF-α的影响

目的：观察牙周基础治疗对2型糖尿病伴牙周炎（DMCP）患者牙周状况、糖代谢及血清肿瘤坏死因子-α（TNF—α）的影响,探讨其可能的影响机制。方法：选取DMCP患者和不伴有全身系统性疾病的慢性牙周炎（CP）患者各30例进行牙周基础治疗。在治疗前及治疗后1、3、6个月分别记录所有患者牙周临床指数：探诊深度（PD）,附着丧失（AL）及探诊出血（BOP）,并检测血清空腹血糖（FBG）、糖化血红蛋白（HbAle）及TNF—α的水平。结果：DMCP组和CP组中PD、BOP和血清TNF—α水平在治疗后1个月、3个月和6个月时均显著降低（P〈0．05）,且CP组下降更显著（P〈0．05）。AL在治疗后3个月开始显著降低（P〈0．05）,但两组间下降值无显著性差异。DMCP组血清FBG、HbAIc水平在治疗后3、6个月显著降低（P〈0．05）。结论：牙周基础治疗能够明显改善牙周状况,并在一定程度上影响患者糖代谢水平。     

慢性牙周炎患者IL-17mRNA的表达及意义

目的：观察TL-17mRNA基因在慢性牙刷炎和健康牙龈组织中的表达水平变化,探讨TL-17在慢性牙周炎发生发展巾的作用。方法：选择慢性牙周炎患者23例,正常对照组15例,记录才周临床指标,采用Real—time PCR方法定量检测TL-17mRNA在牙龈组织中的表达。结果：慢性牙周炎组TL-17mRNA相对表达晕（0．00147＋0．00055）显著高于正常牙龈组（O．00047±0．00019）（P〈0．01）,并且慢性牙周炎组TL-17mRNA表达水平与牙龈指数（r=0．58,P〈0．01）、牙周袋探诊深度（r=0．57,P〈0．01）、附着丧失水平（r=0．49,P〈0．05）呈正相关。结论：Th17相关细胞凶子IL—17呵能住慢性牙周炎发病机制中发挥致炎作用。     

超声龈下洁治和手工龈下刮治在慢性牙周炎治疗中疗效的比较

选取2014-01～2014-12慢性牙周炎患者80例,分2组,分别使用超声龈下洁治（超声组）和手工龈下刮治（手工组）的方法进行治疗,发现在牙周治疗术后4周和12周时2组患者的菌斑指数（PLI）、牙龈出血指数（GBI）、牙周袋深度（PD）和附着水平（CAL）等牙周指标较治疗前均显著下降（P ＜0．05）,超声组治疗后菌斑指数小于手工组（P ＜0．05）,余各项牙周指标在2组患者间均无统计学差异（P ＞0．05）。     

Association of Gingival Crevicular Fluid Cortisol/Dehydroepiandrosterone Levels With Periodontal Status

Background: The aim of the present study is to examine whether anxiety and depression scale scores change with regard to clinical periodontal status and to investigate the association between the levels of stress‐related hormones in gingival crevicular fluid (GCF) and extent/severity of periodontal disease. Methods: One hundred twenty participants who fulfilled the study inclusion criteria were chosen. Patients with chronic periodontitis (CP) and those with healthy periodontal tissues/mild gingivitis were included. The clinical examinations were performed on the day after the psychologic evaluations which included anxiety and depression measurements. GCF sampling was undertaken the following day. Commercially available enzyme‐linked immunosorbent assay kits were used to determine GCF cortisol and dehydroepiandrosterone (DHEA) levels. Study groups were assigned as follows: group 1, non‐periodontitis; group 2, localized CP; and group 3, generalized CP. Results: There were no significant differences with respect to age, sex, education, income level, occupation, or smoking history among the groups (P >0.05). There were no significant differences between the non‐periodontitis and CP groups for any of the psychosocial scales (P >0.05). Group 3 had significantly higher mean DHEA scores compared with group 1 (P <0.05); however, the median cortisol scores showed no statistically significant differences among the three groups (P >0.05). Conclusions: Anxiety/depression scores and GCF cortisol levels did not show any difference with regard to clinical periodontal status. However, a significant association was found between elevated levels of GCF DHEA and the severity of periodontitis.     

Association between psychosocial factors and periodontitis: a case-control study

OBJECTIVE: This case-control study investigated the association between life events, anxiety, and depression with periodontitis. METHODOLOGY: The study counted with 165 individuals, both sexes, 35-60 years of age. Case group included 96 individuals suffering from periodontal disease; and in control group 69 subjects with no history of periodontitis. Clinical examinations were performed by a single examiner. Psychological assessment included four inventories: Life Events Scale, Beck Anxiety Inventory, State-Trait Anxiety Inventory and Beck Depression Inventory. Bivariate and multivariate logistic regression analyses were performed to compare cases and controls. RESULTS: Mean probing depth and clinical attachment level were 3.44+/-0.80 and 4.01+/-1.61 in the case group and 1.96+/-0.19 and 0.95+/-0.50 in the control group, respectively (p<0.05). Positive association of periodontitis with age (odds ratio (OR)=1.15 95% confidence interval (CI): 1.06-1.24), male gender (OR=2.71, CI: 1.13-6.49), smoking (OR=6.05, CI: 1.67-21.94) and educational level (OR=6.49, CI: 1.14-36.95) was confirmed. Bivariate analysis did not demonstrate significant mean differences in life events, anxiety symptoms, trait or state of anxiety, or depression symptoms between cases and controls. Multivariate logistic regression, controlling for confounding factors, demonstrated no significant association between psychosocial factors and periodontal disease. CONCLUSIONS: Within the limits of this study it is possible to conclude that there was no significant association between periodontitis and the psychosocial factors analysed.     

农村唇腭裂患者心理健康状况调查研究

目的:了解农村成年唇腭裂患者的心理健康状况,为改善患者的心理健康状况提供理论依据。方法:采用SCL-90症状自评量表对78名农村成年唇腭裂患者及50名农村正常成年人的心理健康状况进行评价。结果:农村成年唇腭裂患者SCL-90总分、阳性项目数及抑郁、焦虑和人际关系诸因子分高于对照组,差异有统计学意义。其中唇裂(CL)、腭裂(CP)、唇腭裂(CLP)三亚组患者之间的心理健康状况差异无统计学意义。唇裂组不同性别之间在SCL-90总分、阳性项目数及抑郁、焦虑诸因子分存在差异,差异有统计学意义。治疗满意度与患者的心理健康状况显著相关。结论:农村成年唇腭裂患者的心理健康状况较差,对唇腭裂患者临床治疗的同时,要重视社会、心理等因素。     

IL-10mRNA及其蛋白在慢性牙周炎牙龈组织中的表达

目的检测IL-10 mRNA及其蛋白在慢性牙周炎牙龈组织中的表达及其组织细胞来源.方法随机选择12例慢性牙周炎翻瓣术患者作为牙周炎组,10例龈切术患者作为牙龈炎组,6例阻生牙拔除术患者作为健康对照组.分别采用原位杂交和免疫组化检测技术,检测各组牙龈标本中IL-10的表达.每组IL-10 mRNA及蛋白两种水平间的比较采用秩和检验;各组间数据的两两比较采用单因素方差分析.结果IL-10 mRNA及其蛋白在牙周局部牙龈组织均有表达,表达细胞类型有淋巴细胞、浆细胞、巨噬细胞及成纤维细胞等.IL-10在mRNA水平及蛋白水平表达无显著差异(P＞0.05)(牙龈炎组P＜0.05).牙周炎组IL-10表达强度显著高于健康对照组和牙龈炎组(P＜0.01),牙周炎组IL-10 mRNA表达强度显著高于健康对照组(P＜0.01),但与牙龈炎组比较差异无显著性(P＞0.05).结论IL-10在牙周组织中存在局部分泌机制.     

应激对豚鼠牙周炎模型的影响

目的探讨应激对豚鼠牙周炎模型的影响。方法龈沟接种伴放线放线杆菌和牙龈卟啉单胞菌,制备豚鼠牙周炎模型24只,随机分为应激组12只(冷刺激和惊吓刺激)和对照组12只(无刺激),于1、2、4、6周后分批处死,进行临床参数、病理切片、破骨细胞和成骨细胞计数以及血清皮质醇浓度检测。结果应激组第1、2、4周皮质醇浓度升高,第2、4周牙周袋深度与对照组比较有明显差异(P<0.05或P <0.01)。病理切片显示:与对照组相比,应激组牙周破坏更明显,骨修复不活跃,应激组第1、4、6周破骨细胞计数明显增高(P<0.05)。结论应激加重致病菌感染后牙周组织的破坏,延缓组织修复,是牙周炎的重要危险因子。     

Psychosocial stressors in oral lichen planus

BACKGROUND: Psychosomatic factors and their association with dermatological disorders are well recognized, yet their importance in oral lichen planus (OLP) is still debated. This study was done to determine the importance of psychosocial stressors in patients with OLP. METHODS: An analytical age-sex matched double controlled study. Stratified serial random sampling was done for sample selection. The experimental group consisted of biopsy proven OLP subjects (n = 41), negative control consisted of apparently healthy general Out Patient Department (OPD) patients (n = 73) and positive controls were patients with burning mouth syndrome, atypical facial pain and myofacial pain dysfunction syndrome (n = 36). The General Health Questionnaire-version 28 (GHQ-28) and the Hospital Anxiety and Depression Scale (HADS) were used to evaluate psychosocial stressors in terms of stress, anxiety and depression respectively. RESULTS: The OLP patients had significantly higher stress (Z = 4.331; p < 0.05, significant), anxiety (Z = 4.260; p < 0.05, significant) and depression levels (Z = 4.942; p < 0.05, significant) than the negative controls when measured by GHQ and HADS respectively. Similar findings were noted between the two control groups, with higher stress (Z = 5.784; p < 0.05, significant), anxiety (Z = 6.416; p < 0.05, significant), and depression (Z = 4.841; p < 0.05, significant) levels in the positive control group. However, differences in stress (Z = 2.0416; p > 0.05, non-significant), anxiety (Z = 1.681; p > 0.05, non-significant) and depression levels (Z = 0.195; p > 0.05, non-significant) were found to be non-significant between OLP and positive control. CONCLUSION: Significantly higher stress, anxiety and depression levels were found in the OLP and positive control than the general population. No significant difference was noted between the OLP and the positive controls (in which psychosocial stressors are the sole etiology). These suggest that psychological stressors play an important role in the causation of OLP. It may be further hypothesized that these stressors form a starting point for the initiation of various autoimmune reactions, which have been shown to be contributory to the pathogenesis of OLP. Further longitudinal studies need to be done globally before definitive conclusions can be drawn.     

应激性唾液标志物与牙周炎临床参数的相关性分析

目的：探讨应激性唾液标志物嗜铬粒蛋白A（chromogranin A,CgA）、皮质醇、α淀粉酶、β内啡肽与牙周炎临床参数龈沟出血指数（sulcus bleeding index,SBI）、牙周探诊深度（periodontal probing depth,PD）、附着丧失（attachment loss,AL）的相关性。方法：选择2017年12月-2018年12月江西省中西医结合医院口腔科收治的慢性牙周炎患者105例为牙周炎组,同期在院健康体检者105例为对照组,检测2组唾液中CgA、皮质醇、α淀粉酶、β内啡肽水平及SBI、PD、AL。采用SPSS 22.0软件包分析应激性唾液标志物与牙周炎临床参数的相关性,利用多因素Logistic回归分析明确牙周炎有关的危险因素。结果：牙周炎组唾液中CgA、皮质醇、α淀粉酶、β内啡肽水平显著高于对照组(P<0.05),牙周炎组SBI、PD、AL显著高于对照组(P<0.05),唾液中CgA、皮质醇、α淀粉酶、β内啡肽水平与SBI均呈正相关 (P<0.05）,唾液中CgA、皮质醇、α淀粉酶、β内啡肽水平与PD均呈正相关 (P<0.05）,唾液中CgA、皮质醇、α淀粉酶、β内啡肽水平与AL均呈正相关 (P<0.05）,CgA、皮质醇、α淀粉酶、β内啡肽是牙周炎的独立危险因素(P<0.05)。结论：应激性唾液标志物CgA、皮质醇、α淀粉酶、β内啡肽与牙周炎临床参数显著相关,提示应激性唾液标志物能在一定程度上反映牙周炎的病情进展,为牙周炎诊断和疗效监测提供有益参考。     

高原地区牙周炎患者血清内脂素和瘦素水平的检测分析

目的 检测青海西宁地区牙周炎患者血清中内脂素(visfatin,VF)与瘦素(leptin,LP)的表达水平及其与牙周炎症指标的相关性.方法 随机选取2019年3—12月就诊的牙周炎患者50例(牙周炎组)和牙周健康者50例(健康对照组),由同一临床医师测量牙周炎症临床指标,包括探诊深度(probing depth,PD...     

Psychosocial factors and adult onset rapidly progressive periodontitis

Abstract On the basis of clinical observations, some periodontologists have suggested an association between psychosocial factors such as depression, stress and anxiety, and adult onset rapidly progressive periodontitis (RPP). This study investigated more formally possible associations between a number of relevant psychosocial factors and RPP The significance of the psychosocial variables was assessed by comparing 3 groups: 50 patients with RPP, 50 patients with routine chronic adult periodontitis (RCAP). and 50 patients without significant periodontal destruction (controls). It was anticipated that the RPP group would show higher levels of psychosocial maladjustment than the RCAP and control groups. A between-subjects multivariate analysis of covariance indicated that the combined psychosocial variables were significantly related to the periodontal diagnosis. 2 psychosocial factors, depression and loneliness, were significant in distinguishing between groups. The RPP group presented significantly increased depression and loneliness compared to the RCAP and control groups. Future research is indicated to further clarify the significance of these psychosocial differences in relation to the onset and progression of RPP.     

A case–control study on etiological factors involved in patients with burning mouth syndrome

Objective:  To evaluate and analyze the risk factors for burning mouth syndrome (BMS).
Methods:  Eighty-seven consecutive patients with BMS and a randomly selected control group ( n  = 82) were comprehensively investigated with a self-designed questionnaire, Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS). A complete blood count and serum sex hormone were also examined in patients with BMS and control subjects. All the data obtained were transferred to a data bank and analyzed statistically in spss 11.5 for windows.
Results:  No statistical difference between the BMS group and the control group was found in blood analyses including white blood cell count, red blood cell count, hemoglobin and platelet count. Among the menopausal or postmenopausal women with BMS, the follicle stimulating hormone (FSH) level was significantly higher, but the estradiol level was significantly lower. The BMS group reported adverse life events more frequently than the control group. Patients with BMS significantly exhibited symptoms of somatization, and both the scores of anxiety, depression in patients with BMS were higher than those of the control group ( P  < 0.05). A regression equation which included six variables had been established by using logistic regression analysis, indicating that the habit of tongue thrusting, lip sucking, periodontitis, smoking, outcome of recent medication, depression were the principal risk factors, among which tongue thrusting was the most significant.
Conclusion:  Our study indicated that BMS may be of psychological origin, and the measures such as refraining from oral parafunctional activities, removing local irritating factors, stopping smoking, good mental health status could help in the prevention of BMS.