双向电泳技术在急性白血病白细胞蛋白质组中的应用

目的：建立急性白血病白细胞蛋白质组学研究的双向电泳技术体系。方法：提取急性白血病白细胞的蛋白质，建立固相pH梯度双向电泳图谱。结果：初步建立了急性白血病白细胞的双向电泳图谱。结论：急性白血病白细胞蛋白质组学研究的双向电泳技术体系的建立，为进一步研究急性白血病的蛋白质组学奠定了基础。     

蛋白质组学技术在新药研究中的应用

由双向电泳、质谱、计算机图像数据处理组成的蛋白质组学的技术体系具有高通量、高分辨率和高重复性的特点，能对微量样品进行全面自动定量分析．并在药物作用靶标、安全性评价、耐药性机制、疾病动物模型研制和中医药现代化等方面有新颖而重要的应用。蛋白质组学技术将在药物研究和开发中带来根本性的变革，我国应尽快将蛋白质组技术用于药物研究中。     

L-02肝细胞蛋白质双向电泳条件的建立

人类蛋白质组学的研究是揭示人类生命活动和疾病机制的最终阶段,运用蛋白质组学技术寻找各种疾病的关键蛋白和标志蛋白,可实现更加及时、准确的诊断[1]。双向电泳技术一个主要的应用领域是“蛋白质组分析”,包括对来自一个样品的大量蛋白质同时进行系统地分离、识别和定量。本实验通过比较几种不同的蛋白处理方法,优化双向电泳条件,找出较佳的样本处理方式,为成功进行双向电泳及质谱鉴定提供基础。1材料与方法1·1细胞株L-02肝细胞购自中国科学院上海细胞生物研究所。1·2试剂与仪器二羟乙基呱嗪乙烷磺酸(HEPES)、噻唑蓝均为美国Sigma产…     

双向电泳技术及其在疫苗研究开发与生产中的应用

双向电泳技术已广泛用于蛋白质组学研究,该技术除了在农业和基础医学领域中有着广泛的应用外,还在疫苗的研究、开发和生产中具有重要的应用前景.此文就双向电泳技术在疫苗抗原及疫苗候选菌株的筛选、基因工程疫苗的研究、抗原呈递及免疫应答机制的研究和疫苗生产过程中批间一致性分析等方面的应用进行了综述.     

双向电泳技术及其在疫苗研究开发与生产中的应用

双向电泳技术已广泛用于蛋白质组学研究,该技术除了在农业和基础医学领域中有着广泛的应用外,还在疫苗的研究、开发和生产中具有重要的应用前景.此文就双向电泳技术在疫苗抗原及疫苗候选菌株的筛选、基因工程疫苗的研究、抗原呈递及免疫应答机制的研究和疫苗生产过程中批间一致性分析等方面的应用进行了综述.     

双向电泳技术及其在疫苗研究开发与生产中的应用

双向电泳技术已广泛用于蛋白质组学研究,该技术除了在农业和基础医学领域中有着广泛的应用外,还在疫苗的研究、开发和生产中具有重要的应用前景.此文就双向电泳技术在疫苗抗原及疫苗候选菌株的筛选、基因工程疫苗的研究、抗原呈递及免疫应答机制的研究和疫苗生产过程中批间一致性分析等方面的应用进行了综述.     

双向电泳技术及其在疫苗研究开发与生产中的应用

双向电泳技术已广泛用于蛋白质组学研究,该技术除了在农业和基础医学领域中有着广泛的应用外,还在疫苗的研究、开发和生产中具有重要的应用前景.此文就双向电泳技术在疫苗抗原及疫苗候选菌株的筛选、基因工程疫苗的研究、抗原呈递及免疫应答机制的研究和疫苗生产过程中批间一致性分析等方面的应用进行了综述.     

双向电泳技术及其在疫苗研究开发与生产中的应用

双向电泳技术已广泛用于蛋白质组学研究,该技术除了在农业和基础医学领域中有着广泛的应用外,还在疫苗的研究、开发和生产中具有重要的应用前景.此文就双向电泳技术在疫苗抗原及疫苗候选菌株的筛选、基因工程疫苗的研究、抗原呈递及免疫应答机制的研究和疫苗生产过程中批间一致性分析等方面的应用进行了综述.     

双向电泳技术及其在疫苗研究开发与生产中的应用

双向电泳技术已广泛用于蛋白质组学研究,该技术除了在农业和基础医学领域中有着广泛的应用外,还在疫苗的研究、开发和生产中具有重要的应用前景.此文就双向电泳技术在疫苗抗原及疫苗候选菌株的筛选、基因工程疫苗的研究、抗原呈递及免疫应答机制的研究和疫苗生产过程中批间一致性分析等方面的应用进行了综述.     

双向电泳技术及其在疫苗研究开发与生产中的应用

双向电泳技术已广泛用于蛋白质组学研究,该技术除了在农业和基础医学领域中有着广泛的应用外,还在疫苗的研究、开发和生产中具有重要的应用前景.此文就双向电泳技术在疫苗抗原及疫苗候选菌株的筛选、基因工程疫苗的研究、抗原呈递及免疫应答机制的研究和疫苗生产过程中批间一致性分析等方面的应用进行了综述.     

Application of proteomic technologies to tumor analysis

The sequencing of the human genome has had an enormous impact on the proteomic analysis of cancer by providing a sequence-based framework for understanding the human proteome of tumor cells, tissues, and biological fluids. There is intense interest in applying proteomic technologies to uncover, at the protein level, processes involved in neoplastic transformation and new biomarkers that correlate with early diagnosis, as well as to accelerate the development of new therapeutic targets. To that effect, new technologies are being developed in order to meet the needs for the high throughput and high sensitivity that is required for cancer-related applications of proteomics. These innovative technologies have greatly enhanced our ability to separate and characterize complex protein mixtures, and have aided our ability to identify proteins with greater sensitivity, thereby providing the groundwork for future scientific breakthroughs and possibly providing impetus for the development of personalized cancer therapy.     

Entering the era of proteomics in rheumatology

Proteomics, the large-scale analysis of proteins of a given cell or tissue, is a fast-emerging field in biomedical research. It has become clear that proteomic approaches can assist in unravelling complex disease pathways and can help in the discovery of protein drug targets. The recent flood of proteomic data demonstrates the furious digging in the quest for the so-called ‘Holy Grail’. However, at present only a limited number of reports describing proteomic studies in rheumatology have been published. This review highlights some recent advances in the field of proteomic techniques. These new techniques, as well as classic approaches, have potential applications in the field of rheumatology. Some of the proteome studies in rheumatology are directed to the discovery of diagnostic proteins in biological fluids, and others are designed to elucidate the pathophysiology of affected target tissues. As discussed in this review, proteomics is an emerging area in rheumatology and holds great potential in this field. There is little doubt that established and new proteomic tools will lead to landmark discoveries in this field, with applications ranging from diagnostics and therapeutic monitoring to the discovery of new therapeutic targets.     

The continuing evolution of shotgun proteomics

Shotgun proteomics has emerged as a powerful approach for the analysis of complex protein mixtures, including biofluids, tissues, cells, organelles or protein complexes. Having evolved from the integration of chromatography and mass spectrometry, innovations in sample preparation, multidimensional chromatography, mass spectrometry and proteomic informatics continually facilitate, enable and challenge shotgun proteomics. As a result, shotgun proteomics continues to evolve and enable new areas of biological research, and is beginning to impact human disease diagnosis and therapeutic intervention.     

Clinical proteomics: translating benchside promise into bedside reality

The ultimate goal of proteomics is to characterize the information flow through protein networks. This information can be a cause, or a consequence, of disease processes. Clinical proteomics is an exciting new subdiscipline of proteomics that involves the application of proteomic technologies at the bedside, and cancer, in particular, is a model disease for studying such applications. Here, we describe proteomic technologies that are being developed to detect cancer earlier, to discover the next generation of targets and imaging biomarkers, and finally to tailor the therapy to the patient.     

Proteomics in developmental toxicology

The objective of this presentation is to review the major proteomic technologies available to developmental toxicologists and, when possible, to provide examples of how various proteomic technologies have been used in developmental toxicology or toxicology in general. The field of proteomics is too broad for us to go into great depth about each technology, so we have attempted to provide brief overviews supplemented with many references that cover the subjects in more detail. Proteomics tools produce a global view of complex biological systems by examining complex protein mixtures using large-scale, high-throughput technologies. These technologies speed up the process of protein separation, quantification, and identification. As an important complement to genomics, proteomics allows for the examination of the entire complement of proteins in an organism, tissue, or cell-type. Current proteomics technologies not only identify protein expression, but also post-translational modifications and protein interactions. The field of proteomics is expanding rapidly to provide greater volume and quality of protein information to help understand the multifaceted nature of biological systems.     

蛋白质组学在吗啡依赖分子机制研究中的应用

目前蛋白质组学技术被广泛应用于生物医学的各种研究领域,而在吗啡依赖分子机制的蛋白质组学研究方面才刚刚起步。该文通过介绍各种蛋白质组学技术在吗啡依赖分子机制研究中的应用及发现一些潜在的吗啡依赖分子标记物,从而肯定了蛋白质组学在研究吗啡依赖分子机制中的重要性。吗啡依赖蛋白质组学的研究策略应包括吗啡依赖动物和细胞模型的建立、选择合适的样品来源以及改进蛋白质组学技术等,为进一步阐明吗啡依赖分子机制和寻找新的治疗药物靶点提供研究思路和新方法。     

Functional proteomics to study protection of the ischaemic myocardium

Mechanisms to reduce the deleterious effects of myocardial ischaemia are of particular clinical importance and have been the focus of intense research for a number of years. Among novel approaches to studying the ischaemic heart, proteomics, or the analysis of all cellular proteins, presents as a powerful method to deconstruct the mechanisms of disease and protection. Specifically, the field of functional proteomics is an emerging application of proteomics that melds aspects of classical proteomics, biochemistry, molecular biology and physiology into an approach that facilitates an understanding of how proteins and protein interactions engender phenotype. This review highlights different types of proteomic applications and provides a prospectus for functional proteomics as a robust vehicle driving drug discovery and design.     

Functional proteomics to study protection of the ischaemic myocardium

Mechanisms to reduce the deleterious effects of myocardial ischaemia are of particular clinical importance and have been the focus of intense research for a number of years. Among novel approaches to studying the ischaemic heart, proteomics, or the analysis of all cellular proteins, presents as a powerful method to deconstruct the mechanisms of disease and protection. Specifically, the field of functional proteomics is an emerging application of proteomics that melds aspects of classical proteomics, biochemistry, molecular biology and physiology into an approach that facilitates an understanding of how proteins and protein interactions engender phenotype. This review highlights different types of proteomic applications and provides a prospectus for functional proteomics as a robust vehicle driving drug discovery and design.     

Proteomic profiling from human samples: the body fluid alternative

Proteomics is one of the technologies rapidly changing our approach to drug development. The applications of proteomics, particularly with reference to analysis of body fluid samples, will be described. Proteomic analysis involves the systematic separation, identification and characterisation of proteins present in a biological sample. By comparing the proteins present in diseased samples with those present in normal samples, it is possible to identify changes in expression of proteins that potentially may be related to organ toxicity. Proteomics is regarded as a sister technology to genomics. Although the pattern of gene activity will be abnormal in a tissue with a pathological lesion, there can be a poor correlation between the level of activity of different genes and the relative abundance within the tissue of the corresponding proteins. This is especially true where the mode of action of the test material interferes with protein synthesis and/or post translational modification. Consequently, the information about a pathological process that can be derived at the level of gene activity is incomplete. Proteomics has now made it possible to analyse proteins using high throughput, automated techniques. Although both mRNA and proteomic profiling can be applied to tissue samples, analysis of body fluids (e.g. serum, urine, CSF, synovial fluid) is restricted to proteomics. In these cases the protein composition is derived from many tissues and processes. Proteomic analysis can yield information on disease processes and potential response to treatment. Examples will be presented of the identification of surrogate markers for hepatocellular carcinoma, breast cancer, from cerebrospinal fluid in humans and gentamicin toxicity in the rat.     

Drug target identification and quantitative proteomics

The emerging technologies in proteomic analysis provide great opportunity for the discovery of novel therapeutic drug targets for unmet medical needs through delivering of key information on protein expression, post-translational modifications and protein–protein interactions. This review presents a summary of current quantitative proteomic concepts and mass spectrometric technologies, which enable the acceleration of target discovery. Examples of the strategies and current technologies in the target identification/validation process are provided to illustrate the successful application of proteomics in target identification, in particular for monoclonal antibody therapies. Current bottlenecks and future directions of proteomic studies for target and biomarker identification are also discussed to better facilitate the application of this technology.