狭叶薰衣草药材中迷迭香酸的含量测定

目的建立狭叶薰衣草药材中迷迭香酸的含量测定方法,为其质量控制提供科学依据。方法采用HPLC法测定,色谱条件:Phenomenex ODS-A色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈和2 mL·L-1磷酸二元梯度洗脱;流速:1.0mL·min-1;柱温:30℃;检测波长为350nm。结果测定了10批不同产地市售的狭叶薰衣草药材,迷迭香酸的含量在0.04%⁰.33%之间。结论该方法操作简便,重复性好,可用于规范和控制狭叶薰衣草药材的质量。     

比较研究新疆狭叶薰衣草不同部位挥发油成分

目的:对狭叶薰衣草花、叶及茎中挥发油的化学成分进行分析,为狭叶薰衣草的综合利用提供指导。方法:采集狭叶薰衣草的花、叶及茎,以水蒸气蒸馏法提取挥发油,以GC-MS法分析其化学成分。GC-MS主要分析条件:进样口温度250℃,分流比30∶1,离子源温度200℃,程序升温(在40℃保持2 min,最后以3℃·min-1升温速率升至246℃,保持5 min)。结果:在狭叶薰衣草的花、叶及茎的挥发油中分别检出80、60和16个色谱峰,分别鉴定出69、47、15个化合物,分别占各部位挥发油组分总色谱峰面积的94.01%、86.83%、97.35%,3个部位中共有化合物15个。所含成分多为萜烯类化合物及其含氧衍生物。结论:狭叶薰衣草花中挥发油成分含量最多,茎中成分最少,叶挥发油具有潜在的应用价值。     

薰衣草不同部位中挥发油化学成分的比较

目的 分析比较薰衣草不同部位挥发油的化学成分.方法 采用水蒸气蒸馏法分别提取薰衣草花、茎叶两部位的挥发油,用GC-MS全分析法分析其化学成分,并用峰面积归一化法计算各组分的相对含量.结果 从薰衣草花、叶茎中分别分离鉴定出30种和33种化合物,共鉴定出58种成分,其中,共有组分5种.结论 薰衣草花和茎叶两部位的挥发油成分及含量有着很大的差别,可为薰衣草的质量评价提供参考.     

薰衣草及橙花精油体外抑菌作用的研究

目的探讨薰衣草精油（Lavender）、橙花精油（Neroli）的体外抑菌作用。方法采用纸片扩散法和连续稀释法对精油体外抑菌活性进行测定。结果薰衣草精油对金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌、乙型溶血念球菌和白色念珠菌的平均抑菌圈大小分别为：12.00、9.00、10.00、9.50、12.50 mm,最低抑菌浓度（M IC）分别为：217.95、871.80、435.90、871.80、217.95g.L-1。橙花精油对上述5种菌的平均抑菌圈大小分别为：34.75、8.00、12.00、13.75、36.75 mm,M IC分别为：123.93、991.40、991.40、123.93、123.93 g.L-1。其中薰衣草精油对5种菌的抑菌活性强度为：金黄色葡萄球菌〉白色念珠菌、大肠埃希菌〉乙型溶血念球菌、表皮葡萄球菌,橙花精油对5种菌的抑菌活性强度为：金黄色葡萄球菌、白色念珠菌、乙型溶血念球菌〉大肠埃希菌、表皮葡萄球菌。结论薰衣草精油、橙花精油均有一定程度的抑菌效果,可做为天然抑菌剂开发利用。     

薰衣草精油的化学成分与药理活性

从薰衣草属植物中提取的精油广泛用于化妆品和医药领域,对薰衣草精油的化学成分及镇静催眠、抗菌等方面的研究概况加以综述。     

狭叶荨麻精制多糖含量测定

目的测定黑龙江产狭叶荨麻全草中精制多糖的含量。方法以苯酚-硫酸比色法测定,检测波长490nm。结果黑龙江产狭叶荨麻全草中精制多糖的3批平均含量为64.85%,RSD为1.36%,平均回收率为99.76%,RSD为1.19%,线性范围为2.39~14.34（μg.mL-1）,r=0.9993。结论该方法操作简单,精密度、稳定性及重复性好,结果可靠,可用于狭叶荨麻精制多糖的含量测定。     

复方薰衣草油膏的质量标准研究

目的:建立复方薰衣草油膏的质量标准。方法:采用薄层色谱法(TLC)对制剂中黄芩提取物、薰衣草油进行定性鉴别;采用气相色谱法(GC)对制剂中薄荷素油进行定性鉴别;采用GC法测定制剂中薄荷脑的含量,色谱柱为Agilent DB-WAX毛细管柱,程序升温,进样口温度为250℃,检测器温度为250℃,进样量为1μL,分流比为5∶1,分流进样。结果:黄芩苷提取物、薰衣草油的TLC斑点清晰、分离度好、阴性对照无干扰。薄荷素油供试品色谱图中与对照品色谱峰保留时间位置处有相同的色谱峰。薄荷脑检测进样量线性范围为0.113 4~1.133 5μg(r=0.999 4);精密度、稳定性、重复性试验的RSD≤2.0%;加样回收率为95.40%~99.82%(RSD=1.61%,n=6)。结论:所建质量标准可用于复方薰衣草油膏的质量控制。     

薰衣草花水煎液对大鼠离体胸主动脉舒张作用机制研究

目的观察薰衣草花提液对大鼠胸主动脉的血管环舒张作用及可能的机制。方法采用离体大鼠胸主动脉环张力测定法。结果①100.0～700.0mg/L的薰衣草花水煎液对内皮完整的血管环的基础张力没有影响。②100.0~700.0mg/L薰衣草花水煎液对KCl(60mmol/L)所致血管收缩没有明显影响,100.0～500.0mg/L的薰衣草花水煎液对去甲肾上腺素(NE,10μmol/L)预收缩的内皮完整血管环产生舒张作用,100.0～700.0mg/L的薰衣草花水煎液对去甲肾上腺素(NE,10μmol/L)预收缩的去内皮血管环产生舒张作用,一氧化氮合酶抑制剂左旋硝基精氨酸甲酯L-NAME(0.1mmol/L)、鸟苷酸环化酶抑制剂亚甲蓝(10μmol/L)预处理后均可明显抑制薰衣草花水煎液的血管舒张作用,环氧化酶抑制剂吲哚美辛(10μmol/L)预处理后不能抑制薰衣草花水煎液的血管舒张作用。③薰衣草花水煎液预处理去除内皮血管环后,能够明显抑制细胞外钙内流所致的血管收缩,但对细胞内钙释放引起的血管收缩没有影响。结论薰衣草花水煎液对血管舒张的作用是部分依赖内皮,部分不依赖内皮,一方面可通过内皮依赖性的NO-cGMP通路产生血管舒张作用,另一方面通过抑制血管平滑肌细胞膜上受体依赖性钙通道产生血管舒张作用。     

肾叶打碗花总黄酮含量的分析

目的分析肾叶打碗花中的黄酮含量。方法以芦丁作为对照品,利用紫外-可见分光光度法对其进行黄酮含量测定。结果肾叶打碗花黄酮含量为5.73%。结论肾叶打碗花黄酮含量较高。     

HPLC同时测定珠子草中没食子酸、短叶苏木酚和鞣花酸的含量

目的 建立同时测定珠子草中没食子酸、短叶苏木酚和鞣花酸的HPLC法,并对珠子草该3种成分进行含量测定。方法 色谱柱：Shimazu C₁₈(150 mm×4.6 mm,5 μm);流动相：0.1%磷酸(A)-乙腈(B)梯度洗脱,流速为0.8 mL·min^-1;柱温：室温;检测波长：270 nm;进样量10 μL。结果 没食子酸、短叶苏木酚和鞣花酸含量分别在0.6~9.6 μg、0.525~8.4 μg、0.475~7.6 μg内与峰面积呈良好的线性关系。没食子酸的平均含量为0.196 2%,短叶苏木酚为0.518 2%,鞣花酸为0.411 0%。结论 本方法操作简便,准确度高,可用于珠子草中没食子酸、短叶苏木酚和鞣花酸3种成分含量的测定。     

Local anaesthetic activity of the essential oil of Lavandula angustifolia

In this work we studied the local anaesthetic activity of the essential oil obtained from Lavandula angustifolia Mill., a medicinal plant traditionally used as an antispasmodic. We compared its activity to the essential oils obtained from two citrus fruits, Citrus reticulata Blanco and Citrus limon (L.) Burm. f., which have no medical uses. Biological tests were also performed on the major pure components of L. angustifolia Mill. essential oil: linalol and linalyl acetate as determined by GC and confirmed by GC-MS. Anaesthetic activity was evaluated in vivo in the rabbit conjunctival reflex test, and in vitro in a rat phrenic nerve-hemidiaphragm preparation. The essential oil of L. angustifolia, linalyl acetate and linanol (0.01-10 micrograms/ml) but not the oils of Citrus reticulata and Citrus limon were able to drastically reduce, in a dose-dependent manner, the electrically evoked contractions of rat phrenic-hemidiaphragm. In the rabbit conjunctival reflex test treatment with a solution of essential oil of L. angustifolia, as well as linalyl acetate and linalol (30-2500 micrograms/ml administered in the conjunctival sac) allow a dose-dependent increase in the number of stimuli necessary to provoke the reflex, thus confirming in vivo the local anaesthetic activity observed in vitro.     

Analysis of the volatile compounds of flowers and essential oils from Lavandula angustifolia cultivated in Northeastern Italy by headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry

Headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography and mass spectrometry using the divinyl/carboxen/polydimethylsiloxane (DVB-CAR-PDMS) fibre was applied for the analysis of aroma profiles of Lavandula angustifolia L. flowers and the corresponding essential oils. The optimal sampling time was determined by studying the equilibrium time profile of the major volatile compounds for the lavender flowers (50 min) and the essential oil (20 min). Comparative analysis of L. Angustifolia L. cultivated in Friuli Venezia Giulia (northeastern Italy) highlighted that the contents of linalool and linalyl acetate were the major differences between the composition of flowers and the hydro-distilled products. Lavender essential oil from Middle-Friuli Venezia Giulia was evaluated as the highest quality for its high level of linalyl acetate (31.7 %) and linalool (45.0 %) and low percentage of camphor (0.5 %). The use of headspace SPME was shown to be a convenient and effective analytical tool for the sampling of volatile compounds and it could be used to test the quality of flowers and essential oils from Lavandula species.     

基于ITS2序列鉴别维吾尔药材薰衣草及其混伪品

建立了基于ITS2序列鉴别维吾尔药材薰衣草及其混伪品(全叶青兰和夏枯草)的方法.对维吾尔药材薰衣草及其混伪品的ITS2 序列进行PCR扩增和双向测序,使用CodonCode Aligner软件对测序峰图进行序列拼接,用MEGA 6.0 软件对拼接后的序列进行多重比对.并计算种内、种间遗传距离,构建NJ 系统聚类树,预测...     

苘麻子及常见近似品的鉴别

目的： 对苘麻子及近似品野葵子、冬葵子、蜀葵子、锦葵子和黄葵子进行鉴别,为苘麻子及近似品的鉴别提供实验依据。方法：运用性状鉴别和显微鉴别法,分析苘麻子及近似品的特征差异。结果：苘麻子及近似品在性状、显微上均有区别,可以将形状、大小、毛被情况及表面特征作为苘麻子的性状鉴别指标;毛茸的有无、下皮层有无分化、色素层细胞宽度以及子叶有无草酸钙簇晶作为苘麻子的显微鉴别指标。结论：以上结果可作为苘麻子及近似品野葵子、冬葵子、蜀葵子、锦葵子和黄葵子的主要鉴别依据。     

Three new benzolactones from Lavandula angustifolia and their bioactivities

Three new benzolactones (1–3), together with four known ones (4–7), were isolated from the whole herb of Lavandula angustifolia. Their structures were established on the basis of detailed spectroscopic analysis (1D- and 2D-NMR, HRESIMS, UV, and IR) and comparison with data reported in the literature. New compounds were evaluated for their anti-tobacco mosaic virus (TMV) activities and cytotoxic activities. The results revealed that compounds 1–3 showed obvious anti-TMV activities with inhibition rates of 26.9, 30.2, and 28.4%, which were at the same grade as positive control. Compounds 1–3 also showed weak inhibitory activities against some tested human tumor cell lines with IC₅₀ values in the range of 32.1–7.6 μM.     

薰衣草对神经系统药理作用的研究进展

薰衣草有"香草之后"之称,药用历史悠久,被用于多种神经疾病的治疗。以国内外对薰衣草的广泛研究为基础,从动物试验及相关机制、临床试验两方面进行总结。薰衣草在焦虑、失眠、老年痴呆、中风、抑郁、疼痛等多种神经系统疾病具有显著的疗效,是神经药物领域重要的药用资源,具有广阔的开发前景。     

滇南金线兰的植物形态和显微鉴定

目的 对滇南金线兰进行生药鉴定,明确其原植物形态和显微特征。方法 采用生药学鉴定方法,观察滇南金线兰的原植物形态、组织构造及粉末显微特征。结果 叶片呈卵形或卵状披针形,具金红色叶脉,花不倒置,唇瓣黄色,呈Y字形,前部明显扩大并2裂,裂片狭长圆形或狭倒披针形,中部收狭成长10 mm左右、其边缘具狭翅。显微结构中,根、茎横切面中皮层明显,具草酸钙针晶、黏液细胞等;叶横切面上表皮细胞乳突状,下表皮气孔类型多样,以不定式气孔为主。粉末中可见草酸钙针晶和导管。结论 滇南金线兰的生药鉴定为其资源开发利用提供了参考依据。     

猪殃殃的生药学研究

目的研究猪殃殃的生药鉴定特征。方法对猪殃殃的药材性状、显微特征及理化性质进行研究。结果详细描述了猪殃殃全苹的生药鉴定特征及其所含的各类成分。结论猪殃殃具有独特的生药学特征,可为其鉴定、开发利用及质量标准的制订提供依据。     

The antimutagenic activity of Lavandula angustifolia (lavender) essential oil in the bacterial reverse mutation assay.

Essential oils from Melaleuca alternifolia (tea-tree oil) and Lavandula angustifolia (lavender oil) are commonly used to treat minor health problems. Tea-tree oil possesses broad-spectrum antimicrobial activity, and is increasingly used for skin problems. Lavender oil, traditionally used as an antiseptic agent, is now predominantly used as a relaxant, carminative, and sedative in aromatherapy. Despite their growing use no data are available on their mutagenic potential. In this study, after determining the chemical composition of tea-tree oil and lavender oil, by gas-chromatography and mass spectrometry, we investigated their mutagenic and antimutagenic activities by the bacterial reverse mutation assay in Salmonella typhimurium TA98 and TA100 strains and in Escherichia coli WP2 uvrA strain, with and without an extrinsic metabolic activation system. Neither essential oil had mutagenic activity on the two tested Salmonella strains or on E. coli, with or without the metabolic activation system. Conversely, lavender oil exerted strong antimutagenic activity, reducing mutant colonies in the TA98 strain exposed to the direct mutagen 2-nitrofluorene. Antimutagenicity was concentration-dependent: the maximal concentration (0.80 mg/plate) reduced the number of histidine-independent revertant colonies by 66.4%. Lavender oil (0.80 mg/plate) also showed moderate antimutagenicity against the TA98 strain exposed to the direct mutagen 1-nitropyrene. Its antimutagenic property makes lavender oil a promising candidate for new applications in human healthcare.