“互联网+”实验动物资源共享服务平台模式研究

本文是在调查和梳理当前我国实验动物行业资源以及实验动物资源供销现状的基础上,分析实验动物行业用户群体对实验动物资源信息化服务的应用需求,探索研究在"互联网+"环境下我国实验动物资源共享服务平台模式,充分发挥"互联网+"在社会资源配置中的优化和集成作用,助力实验动物行业资源的共建共享。文章重点以一站式实验动物资源共享服务平台为例,介绍了平台的设计思路和功能模块的实现。     

实验动物从业人员培训系统的建立与应用

实验动物是生命科学、医学研究的重要组成部分,是国家科技创新的重要支撑。实验动物科学对保障人类健康、食品安全和生物安全具有重要意义。食品安全、药物、疫苗、生物制品开发以及人类疾病发病机理研究等,都离不开实验动物。为适应中国实验动物行业的整体发展需求,本单位自主研发了"实验动物从业人员网络培训系统",该系统是一套集实验动物实际操作和理论知识于一体的网络教育培训平台,包括动物福利、动物饲养、动物手术等7个知识模块,以管理人员、实验人员、实验动物医师和饲养人员为培训对象,旨在加速实验动物的人才培养和团队建设,促进行业培训的转型发展。     

实验动物智能化综合管理系统开发及应用

目的 为了提高医科院校实验动物管理效率和用户体验,以探索实验动物综合管理的智能化、科学化和流程化。方法 应用计算机、物联网云平台和移动通信终端等技术构建一套用于实验动物生产、销售、动物实验、数据统计与分析等环节于一体的智能化综合管理系统。结果 该系统经过测试和试运行,实现了实验动物综合管理系统各主要技术流程和环节的稳定运行。结论 本系统运行安全稳定、性能可靠,实现智能化的管理模式,降低了运行成本,达到了系统开发的预期效果。     

基于移动互联网的实验动物商城平台的探讨

通过分析实验动物行业特点、行业供需现状以及移动互联网应用前景,以"移动互联网+实验动物"相结合,研究实验动物商城平台的功能和作用及其运营模式。平台将以开放性为实验动物行业提供电商服务,形成"资源共享、互利共赢"的创新型格局,促进实验动物产业链资源的均衡配置,营造效能、效率、效果良好的运行管理环境。     

数字时代中医药图书馆知识咨询服务模式研究

知识咨询服务是为用户提供基于信息分析与内容的服务,是参考咨询服务的变革与延伸,亦是数字时代中医药图书馆更高层次的信息服务。在阐述知识咨询服务的背景、内涵及知识咨询服务特征的基础上,对知识咨询服务的主要模式进行了探讨研究。     

在线医疗社区信息咨询服务的用户感知价值研究

目的：对我国在线医疗社区信息咨询服务的用户感知价值进行综合评价，为在线医疗社区的运营管理提供借鉴。方法：基于感知价值理论及在线信息服务情境特征，构建在线医疗社区信息咨询服务的用户感知价值评价指标体系。通过专家访谈法确定指标权重，运用模糊综合评价法对在线医疗社区信息咨询服务的用户感知价值进行评价分析。结果：“好大夫在线”信息咨询服务总体评价较高，货币成本、功能价值和在线医生价值是影响在线医疗社区信息咨询服务用户感知价值的重要因素。结论：在线医疗社区平台的运营需注重平台功能的设计与优化，加强在线医生的管理，保护用户隐私，提升用户信任度。     

中国替代方法研究评价中心共识平台的建立

在社会需要和科技进步的双重推动下,"3R"原则和动物试验替代方法正在从道德理念向实验技术转变。站在全球科技发展的高度,结合我国实验动物替代领域的现状和未来趋势,利用MSSQLServer2000构建中国替代方法研究评价中心共识平台,管理信息数据库,用WEB技术将数据库与internet WWW(WorldWideWeb)页面连接,构成动态的咨询平台和信息数据网络查询系统。该共识平台的建立为国内从事实验动物及替代方法研究应用的用户提供了便利,起到了国内外替代技术交流的桥梁作用。     

上海实验用兔供应的社会化正在起步

经过4年建设、在多方面关心和支持下不断成长的上海陈行实验用兔繁育场,于1986年9月19日在上海科学会堂主持召开关于实验用兔质量征询和1987年度供应计划的座谈会。与会代表有市科委、市卫生局、市医药局、市实验动物科学研究会、市医学实验动物管理委员会和上海实验动物研究中心的有关负责同志和实验动物科技专家,以及有关行业的用户代表,共计120人。由乔场长汇报了建场4年来的生产供应情况;市实验动物科学研究会的专家报告了对该场开展咨     

某三甲医院孕妇网络健康信息搜寻行为调查

用在线问卷调查法调查了孕妇这一用户群体的网络健康信息搜寻行为,用SPSS 24.0进行统计分析的结果显示,93%的孕妇会主动通过互联网获取健康信息,主要目的是丰富孕期知识,主要内容是营养膳食信息,主要途径是手机APP、网站和微信公众号,主要评价依据为发布信息的平台和发布信息的目的。互联网已经成为孕妇健康消费最流行的信息来源之一,因此政府机关和主流媒体要分别做好信息发布平台的监管与引导工作,卫生行政部门和医疗机构应发挥网络健康信息传播的优势,医疗行业专家要成为互联网医疗的活跃用户,孕妇自身也要提高网络健康信息素养。     

高校在实验动物产业化中的角色思考

通过对高校实验动物机构职能演变的概述,对高校从传统实验动物产业向现代实验动物产业转化进程中的角色转换进行了思考,分析了我国实验动物产业化的现状,认为重构我国实验动物产业化发展模式势在必行。高校作为产、学、研联合体的重要环节,在实验动物产业化的进程中应当承担学术的"召集人"、企业的"金手指"、行业的"协调者"、平台的"设计师"等角色。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.