结肠癌组织中Fas表达的定量分析

目的：探讨Fas在结肠癌中的表达及其与肿瘤发生、发展及肿瘤分化程度的关系。方法：用直接免疫荧光－流式细胞技术对50例结肠腺癌组织及手术切缘的正常结肠黏膜组织中Fas的表达进行定量检测，分析Fas表达与肿瘤大体类型、浸润深度、分化程度及淋巴结转移的关系。结果：Fas在结肠癌组织中的平均阳性率及平均荧光指数均低于其手术切缘的正常结肠黏膜组织，在12例伴有转移的结肠癌组织中Fas的平均阳性率及平均荧光指数均低于38例没有转移的结肠癌；9例肿瘤限于肌层以内的结肠癌组织中Fas平均阳性率及平均荧光指数均高于41例肿瘤侵及肌层以外的结肠癌病例。不同分化程度、不同大体类型的结肠癌组织中Fas的平均阳性率及平均荧光指数差异无显著性。结论：结肠腺癌组织中Fas的表达受到了抑制，Fas的表达与肿瘤的转移及浸润深度呈负相关，与肿瘤的分化程度及大体类型无关。     

宫颈癌中MMP-2及TIMP-2的表达及其临床意义

目的 :通过检测基质金属蛋白酶 - 2 (MMP - 2 )及基质金属蛋白酶组织抑制物 - 2 (TIMP - 2 )在宫颈癌中的表达 ,探讨其与宫颈癌侵袭转移的关系。方法 :采用免疫组化S -P法检测 5 1例宫颈癌和 16例正常宫颈组织中MMP - 2和TIMP - 2的表达情况。结果 :MMP - 2、TIMP - 2在正常宫颈上皮组织中均无表达 ,在宫颈癌组织中的阳性表达率分别为 74 .5 % (38/ 5 1)、4 7.1% (2 4 / 5 1) ,有显著性差异 (P <0 .0 1)。MMP - 2、TIMP - 2的表达与组织学类型无关 ,但与临床分期、细胞分化程度、淋巴结转移有关。结论 :MMP - 2、TIMP - 2的表达与宫颈癌的侵袭转移有关 ,MMP - 2、TIMP - 2可作为预测宫颈癌侵袭转移潜能和临床预后的指标。     

Kiss-1及基质金属蛋白酶9在结肠癌组织中的表达及意义

目的检测Kiss-1及基质金属蛋白酶9(MMP-9)在结肠癌组织中的表达,探讨其临床意义。方法采用链霉菌抗生物素蛋白-过氧化酶连接(SP)法检测68例结肠癌,26例结肠腺瘤及26例正常结肠组织中Kiss-1及MMP-9的表达情况,分析其与各种临床病理参数的关系及两者表达的关联性。结果Kiss-1蛋白metastin在结肠癌组中的表达率(48.5%)明显低于结肠腺瘤组(80.8%)及正常结肠组(96.2%),并且其在结肠癌组中的表达率在不同的组织学分级、肌层浸润深度及淋巴结转移中差异有统计学意义(P均<0.05)。MMP-9在结肠癌组中的表达率(75%)明显高于结肠腺瘤组(38.5%)和正常结肠组(15.4%),其在结肠癌组中的表达率在不同的肌层浸润深度、淋巴转移中差异有统计学意义(P均<0.05)。Kiss-1蛋白metastin与MMP-9在结肠癌中的表达呈相反的趋势,有关联性(P<0.01)。结论Kiss-1蛋白metastin的表达下调和MMP-9的表达上调可能与结肠癌的浸润、转移有关。     

神经源性分化蛋白在胰腺癌中的表达和意义

目的 探讨神经源性分化蛋白(NeuroD)在胰腺外分泌癌中的表达及其意义.方法 应用组织芯片和免疫组织化学EnVision二步法研究NeuroD、增殖细胞核抗原(PCNA)、p53在127例胰腺外分泌癌中的表达情况,并在光镜下观察胰腺癌神经组织浸润、神经周围淋巴细胞套、癌旁慢性胰腺炎、胰周淋巴结转移情况.分析NeuroD的表达与上述其他指标及性别、年龄、肿瘤部位、肿瘤组织学类型及分化程度等的关系.结果 NeuroD、PCNA和p53蛋白在胰腺癌组织中的阳性率分别为64.6％(82/127)、57.5％ (73/127)和59.1％ (75/127),与菲癌组织[分别为10.5％ (8/76)、9.2％(7/76)和9.2％(7/76)]相比,差异有统计学意义(P＜0.01).NeuroD蛋白的表达与PCNA、p53蛋白的表达和胰腺癌肿瘤神经浸润之间有统计学相关性(P＜0.05),而与性别、年龄、肿瘤部位、肿瘤组织学类型及分化程度、癌旁慢性胰腺炎、神经周围淋巴细胞套和淋巴结转移均等无统计学相关性(P＞0.05).结论 NeuroD蛋白在胰腺癌中呈高表达,可能参与了胰腺癌的发生和进展,并且与胰腺癌的增殖、p53信号通路和肿瘤神经浸润之间密切相关.     

Comparative study on the expression of the blood group antigens Le a,Le b,Le x,Le y and the carbohydrate antigens CA 19-9 and CA-50 in chronic pancreatitis and pancreatic carcinoma

Summary The expression of the blood group antigens Le a, Le b, Le x, Le y and the carbohydrate antigens CA 19-9 and CA-50 was studied in 20 ductal pancreatic carcinomas, 24 pancreases with chronic pancreatitis and 10 normal fetal and adult pancreases. CA 19-9, CA-50 and Le a showed the strongest staining intensity, the highest percentage of labelled cells, and a membrane-bound expression pattern in epithelial cells of normal pancreas, chronic pancreatitis and well differentiated (G1) carcinoma; in moderately and poorly differentiated carcinomas (G2/3) it was predominantly cytoplasmic. The staining pattern of Le b and Le x was less clearly membrane-bound but varied with cytoplasmic and Golgi-located distributions in all pancreatic specimens. Le y revealed a consistent granular antigen expression in the Golgi-region of ductal epithelial, acinar and carcinoma cells.None of the antibodies allowed a morphological differentiation by their expression pattern between hyperplastic, metaplastic and dysplastic or neoplastic cells. The differences in their staining patterns were quantitative and did not allow a qualitative differentiation between chronic pancreatitis and pancreatic carcinoma.We found coexpression of Le a and Le b antigens in 46/54 pancreatic specimens. All but 7 pancreata were CA 19-9 positive. An association between Le x, y and Le a, b antigen expression could not be noted in our material.supported by the Johannes and Frieda Marohn Foundation Erlangen     

VEGF—C和MMP-2在直肠癌中的表达及意义

目的：研究血管内皮生成因子-C（VEGF—C）及基质金属蛋白酶-2（MMP-2）在直肠癌中的表达,探讨其与直肠癌浸润、转移的关系,以及两者的表达特征和内在联系。方法：应用免疫组织化学Elivision plus法检测96例直肠癌组织、癌旁组织及正常直肠组织中VEGF—C和MMP-2的表达情况。结果：VEGF—C和MMP-2在正常直肠组织表达率分别为2．1％和1．0％,在癌旁组织中表达为18．7％和21．8％,在直肠癌组织中表达为81．3％和79．2％。两者在直肠癌组织中表达率明显增高（P〈0．05）,且表达率的高低与淋巴结转移、分化程度、浸润程度密切相关（P〈0．05）。结论：VEGF—C和MMP-2在直肠癌中高表达且两者呈正相关,并与直肠癌侵袭和转移性有关,可作为预测直肠癌转移潜能的指标之一。     

水通道蛋白1和微血管密度在子宫颈癌中的表达及临床意义

目的明确水通道蛋白(AQP)1和微血管密度(MVD)在子宫颈上皮内瘤样变(CIN)和子宫颈鳞癌、腺癌组织中的表达和分布,探讨其在子宫颈癌发病中的意义。方法采用免疫组织化学Envision法检测AQP1和MVD在74例子宫颈癌(其中子宫颈鳞癌46例,子宫颈腺癌28例),34例CIN和15例正常子宫颈组织中的表达情况。结果AQP1主要表达于CIN和子宫颈鳞癌、腺癌的血管内皮细胞,以腺癌表达量最大,鳞癌与CIN的表达量基本一致,而在其他各组间都有统计学意义。随着宫颈病变的进展,MVD逐渐增大,各组间比较差异有统计学意义。但发现在子宫颈鳞癌、腺癌和正常组织中MVD的表达量要比AQP1大,并有统计学意义;而在CIN中的表达量相对一致。在子宫颈癌的临床病理特征中,淋巴转移阳性者AQP1和MVD的表达量较阴性者高(P<0.01)。结论AQP1主要表达于子宫颈癌组织间的血管内皮细胞,其表达量较微血管密度低,并与微血管一起在子宫颈癌的生长、侵袭和转移中起重要的作用。     

Immunohistochemical localization of programmed death-1 ligand-1 (PD-L1) in gastric carcinoma and its clinical significance

The present study examined programmed death-1 ligand-1 (PD-L1) detected by immunohistochemical labeling in 102 cases of human gastric carcinoma, 10 adenoma and 10 normal tissues. The relationship between PD-L1 immunolocalization and clinical pathological features, as well as the prognosis of gastric carcinoma, was explored. There was no PD-L1 detectable in normal gastric tissues and very weak immunolabeling in gastric adenomas, but it could be detected in 42.2% of gastric carcinoma tissues. There was no correlation between PD-L1 immunolocalization and patient age, sex, tumor location or the degree of tumor differentiation in the gastric carcinomas. However, PD-L1 immunodetection was significantly correlated to tumor size, invasion, lymph node metastasis and survival time of patients. PD-L1 immunolabeling was significantly enhanced (P<0.01) when the tumor infiltrated into the deep muscular layers, with lymph node metastasis or survival time of less than 2 years, Moreover, multivariate analysis demonstrated that PD-L1 immunodetection could be used as an independent factor to evaluate the prognosis of gastric carcinoma.     

宫颈癌COX-2、MMP-9和CD105表达与肿瘤血管生成及侵袭转移的关系

目的探讨环氧合酶-2(cyclooxygenase,COX-2)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在宫颈癌局部肿瘤血管生成及肿瘤侵袭转移过程中的作用。方法采用免疫组化SP法检测32例正常宫颈上皮及68例宫颈癌中COX-2、MMP-9的表达。并用CD105标记新生血管内皮细胞,计算肿瘤内微血管密度(microvascular density,MVD)。结果COX-2、MMP-9在宫颈癌组织中的阳性表达率均显著高于正常宫颈上皮中的表达(P<0.01)。COX-2、MMP-9阳性组的MVD均显著高于COX-2、MMP-9阴性组(P<0.01),淋巴结转移组COX-2、MMP-9阳性率均高于无淋巴结转移组(P<0.05)。结论COX-2、MMP-9阳性表达可能在宫颈癌血管生成和侵袭转移中起重要作用,两者在宫颈癌血管生成中可能具有协同作用。检测宫颈癌中COX-2、MMP-9表达对进一步了解宫颈癌局部血管生成情况及判断预后有一定的应用价值。     

Prognostic significance of maspin in pancreatic ductal adenocarcinoma: tissue microarray analysis of 223 surgically resected cases.

Maspin (SERPINB5), a serine proteinase inhibitor, was first identified as a potential tumor suppressor on the basis of its differential expression between normal mammary epithelial cells and human breast carcinoma cell lines. Recent studies have shown that maspin might be a prognostic tumor marker. Pancreatic ductal adenocarcinoma acquires maspin expression through hypomethylation of the maspin promoter. However, no study has investigated the prognostic significance of maspin expression in pancreatic ductal adenocarcinomas. In this study, we investigated maspin protein expression in a large series of 223 surgically resected pancreatic ductal adenocarcinomas using immunohistochemical staining and high throughput tissue microarrays. Maspin expression was correlated with postoperative survival and other clinicopathologic factors. Maspin was detected in 209 of these 223 (94% cases) pancreatic ductal adenocarcinomas including 39 (18% cases) focal (5-50% tumor cells) and 170 (76% cases) diffuse (>50% tumor cells). Fourteen (or 6% cases) pancreatic ductal adenocarcinomas did not show maspin expression by immunohistochemical staining (<5% tumor cells). Normal ductal epithelium is not labeled with maspin. Overexpression of maspin in pancreatic ductal adenocarcinoma is associated with worse postoperative survival especially in patients whose tumors exhibit diffuse expression of maspin. After adjusting other clinicopathologic factors, maspin expression remains to be an independent adverse prognosticator for postoperative survival. Maspin expression is not associated with patient age, gender, tumor size, tumor pathologic stage, lymph node status, and vascular invasion or perineural invasion. Nuclear labeling of maspin is associated with better tumor differentiation although this staining pattern is not associated with a better prognosis. In addition, maspin overexpression is also observed in 48% low-grade (grades 1a and 1b) pancreatic intraepithelial neoplasias (PanINs) and 78% high-grade (grades 2 and 3) PanINs, suggesting that maspin upregulation occurs early during the multi-step progression model of pancreatic ductal adenocarcinoma.     

紧密连接蛋白1 mRNA和蛋白表达与胃癌侵袭转移及预后的相关性

目的 探讨紧密连接蛋白1(ZO-1)mRNA及蛋白表达在胃癌侵袭转移及预后中的意义.方法 Real-time qPCR法检测52例胃腺癌细胞ZO-1 mRNA表达,组织芯片SP免疫组织化学法检测228例胃腺癌细胞ZO-1蛋白表达定位变化情况.结果 81.1%的胃癌组织出现不同程度的ZO-1胞膜表达异位至胞质迷离表达的现...     

乳腺良、恶性病变组织中MMP-26蛋白的表达及其与ER的关系

目的检测基质金属蛋白酶-26(MMP-26)蛋白在人乳腺良、恶性病变组织中的表达,及其与部分临床指标的关系,分析MMP-26在肿瘤进展中的作用及临床意义,并探讨雌激素及其受体(ER)对MMP-26蛋白表达调节作用。方法应用免疫组化SP法,检测MMP-26蛋白在正常乳腺组织、乳腺增生症、原位癌和乳腺浸润性导管癌(IDC)中的表达,以及ER在IDC中的表达并进行评分,结果用INSTAT统计软件分析。结果6例正常乳腺组织和8例乳腺增生症中,分别有1例MMP-26呈弱阳性表达,阳性率分别为16.7%和12.5%。4例原位癌中MMP-26全部阳性表达。在67例IDC中,有41例MMP-26阳性表达,阳性率为61.2%。MMP-26的表达与发病年龄、肿瘤大小、病理学分级无关,而与淋巴结转移密切相关(P<0.05)。MMP-26蛋白在IDC的阳性表达与ER在癌组织中的表达呈明显的负相关(P<0.01)。结论MMP-26在肿瘤浸润和转移中发挥重要作用;MMP-26在IDC中的表达可能受雌激素及其受体的调节。     

胃癌中SDF-1、CXCR4、MMP-2和MMP-9的表达及意义

目的研究趋化因子SDF-1及其受体CXCR4以及MMP-2和MMP-9在胃癌中的表达,探讨SDF-1对MMP-2和MMP-9表达的影响。方法应用免疫组化EnVision两步法检测109例胃癌组织中SDF-1、CXCR4、MMP-2和MMP-9的表达。结果 (1)SDF-1、CXCR4、MMP-2、MMP-9在胃癌组的表达阳性率分别为88.1%、56.9%、80.7%和83.4%,高于切缘对照组的47.8%、30.4%、43.4%和47.8%,差异有显著性(P<0.05);(2)SDF-1和CXCR4的表达在淋巴结转移组高于无转移组(P<0.05),SDF-1、MMP-9表达程度与淋巴结转移、组织学分级、浆膜侵犯、临床分期指标呈正相关(P<0.05);MMP-2、CXCR4表达程度与淋巴结转移、浆膜侵犯、临床分期呈正相关(P<0.05);(3)SDF-1与其受体CXCR4的表达及与MMP-2、MMP-9均呈正相关(P<0.05)。结论 (1)SDF-1、CXCR4、MMP-2和MMP-9的表达水平与胃癌的发生、侵袭及淋巴结转移密切相关,可作为预测胃癌淋巴结转移及预后的指标;(2)SDF-1/CXCR4轴可通过加强肿瘤细胞MMP-2和MMP-9分泌的途径促进肿瘤的浸润和转移,提示SDF-1可能是药物靶向治疗的重要靶点。     

31例胰腺癌组织nm23/NDPK的表达及其意义

目的：探讨ｎｍ２３／ＮＤＰＫ癌基因在胰腺癌细胞中的表达及其临床病理学意义。方法：采用免疫组化Ｓ－Ｐ法，对３１例胰腺癌组织及８例正常胰腺组织进行ｎｍ２３／ＮＤＰＫ癌基因表达的检测。结果：３１例胰腺癌组织中有２０例ｎｍ２３／ＮＤＰＫ表达阳性，占６４．５％；８例正常胰腺组织中仅２例ｎｍ２３／ＮＤＰＫ表达阳性，占２５％；两者有显著性差异（Ｐ〈０．０５）。低分化胰腺癌ｎｍ２３／ＮＤＰＫ表达阳性率明显高于高分     

Differential expression of matrix metalloproteinase-13 in mucinous and nonmucinous colorectal carcinomas

Colorectal carcinoma (CRC) is a major health problem all over the world. Mucinous CRCs are known to have a peculiar behavior and genetic derangements. This study aimed to investigate matrix metalloproteinase (MMP)-13 expression in mucinous and nonmucinous CRCs. We studied tumor tissue specimens from 150 patients with mucinous and nonmucinous CRC who underwent radical surgery from January 2007 to January 2012. High-density manual tissue microarrays were constructed using a modified mechanical pencil tip technique, and paraffin sections were submitted for immunohistochemistry using MMP-13. Statistical analysis was performed for clinical and pathological data of all studied cases together with MMP-13 expression in mucinous and nonmucinous groups. Mucinous carcinoma was significantly associated with young age, more depth of invasion, lymph node metastasis, and less peritumoral and intratumoral neutrophils. Nonmucinous carcinomas showed higher MMP-13 expression compared with mucinous carcinomas. Despite the negative or low expression of MMP-13, mucinous carcinomas had more depth of invasion and more frequency of lymph node metastasis than did nonmucinous carcinomas.     

组成型一氧化氮合酶的表达与胃癌的侵袭转移和预后的关系

目的　观察胃癌组织中cNOSmRNA表达分布情况 ,探讨其与胃癌侵袭转移和预后的关系。　方法　运用原位杂交技术 ,检测 119例原发性胃癌标本中cNOSmRNA的表达 ,并对所获得的 82份病例随访资料进行生存分析。　结果　胃癌组织中胃腺癌细胞、血管 淋巴管内皮细胞及巨噬细胞中均可见有cNOSmRNA表达 ;肿瘤细胞分化程度越低、恶性度越高 ,胃腺癌细胞和血管 淋巴管内皮细胞cNOSmRNA阳性表达率就越高 ;胃腺癌细胞和血管 淋巴管内皮细胞cNOSmRNA阳性表达率与胃癌侵袭深度、淋巴结转移以及TNM分期呈正相关 ;胃癌血管 淋巴管内皮细胞cNOSmRNA阳性表达者术后生存率较低 ;巨噬细胞中cNOSmRNA表达与胃癌组织学分型、侵袭深度、淋巴结转移、TNM分期及患者术后生存期无关。　结论　胃腺癌细胞和血管 淋巴管内皮细胞cNOSmRNA表达情况与肿瘤恶性程度、侵袭和转移潜能有关 ,血管 淋巴管内皮细胞上cNOSmRNA阳性表达提示患者预后不良     

Ezrin、MMP-2及TIMP-2在子宫颈癌中的表达与侵袭转移的关系

目的 通过观察埃兹蛋白(Ezrin)、金属蛋白酶-2(MMP-2)及金属蛋白细胞组织抑制因子-2(TIMP-2)蛋白水平在子宫颈癌组织中的表达情况,探讨它们与子宫颈癌侵袭转移的关系. 方法 采用免疫组织化学方法对40例子宫颈鳞癌,20例腺癌,29例子宫颈上皮内瘤样病变和16例正常子宫颈组织进行标记及分析. 结果 Ezrin、MMP-2及TIMP-2蛋白主要表达在子宫颈鳞癌、腺癌、上皮内瘤变的异型细胞质内,各实验组中的表达量与正常组比,差异均有统计学意义(P<0.01).Ezrin和MMP-2蛋白在有淋巴结转移组中的表达量较无淋巴结转移组明显高(P<0.01), 而TIMP-2蛋白水平的表达结果却相反.同时发现Ezrin、MMP-2及TIMP-2蛋白水平在宫颈病变组织表达有正相关性. 结论 Ezrin、MMP-2及TIMP-2 蛋白在子宫颈鳞癌、腺癌的表达量较对照组高. Ezrin和MMP-2在有淋巴结转移的子宫颈癌中高表达,而TIMP-2为低表达,提示它们在子宫颈癌的侵袭转移中起重要的作用.Ezrin、MMP-2及TIMP-2蛋白水平在宫颈病变组织表达有正相关性,这三者在子宫颈癌的侵袭转移中可能起协同作用.     

乳腺癌中E-cadherin的表达及其临床病理意义

目的 探讨上皮钙黏附表（E-cadherin，E-cad）与乳腺癌临床病理和生物学行为的关系。方法 用免疫组化S-P法检测乳腺浸润性导管癌和浸润性小叶癌中E-cad的表达情况及其与淋巴结转移，ER-PR表达水平的相关性。结果 乳腺浸润性导管癌E-cad表达高于浸润性小叶瘤，有淋巴结转移者E-cad表达低于无转移者，E-cad高表达的乳腺浸润性导管癌及浸润性小肾癌中均与ER-PR表达水平成正相关。结论 E-cad中作为乳腺癌分类、转移潜能和预后判断的参考指标。