CD44s和CD15在前列腺癌组织中的表达及其意义

目的 探讨细胞黏附分子CD44s和CD15在前列腺癌(PCa)组织中的表达及其临床意义.方法 采用免疫组化SP法检测42例前列腺癌(PCa)组织和15例良性前列腺增生(BPH)组织中CD44s和CD15的表达,并用图像分析系统测量CD44s和CD15的平均光密度值.结果 (1)CD44s在PCa组织中的表达明显低于BPH组织(p＜0.01);CD44s在PCa组织中的表达随着Gleason分级、临床分期的增加而明显降低(p＜0.01);PCa转移组中CD44s表达明显低于无转移组(p＜0.01).(2)PCa组织中CD15的表达显著高于BPH(p＜0.01),CD15在PCa组织中的表达随着Gleason分级、临床分期的增加而增强(p＜0.001);转移组中CD15的表达显著高于无转移组(p＜0.001).(3)在PCa组织中CD44s与CD15表达呈负相关(r=-0.785,p＜0.001).结论 CD44s和CD15的异常表达在PCa的恶性进展过程中起重要作用,将CD44s和CD15联合分析,可更准确判断肿瘤恶性程度、转移和预后.     

CD44s和CD15在前列腺癌组织中的表达及其意义

Objectives To investigate expression of cell adhesion molecule CD44 sand CD15 in prostate carcinoma(PCa), and to evaluate its clinical significance. Methods Forty two cases of PCa and 15 of benign prostate hyperplasia(BPH) were detected by immunolhistochemical technique and the intensity of CD44s and CD15 expression was assessed by computer image analysis system. Results (1) The intensity of CD44s expression was significantly lower in PCa than that in BPH. PCa showed significant loss of CD44s expression, which correlated with the rise of tumor Gleason grade and clinical stage( p＜0.01 ), The intensity of CD44s expression was significantly lower inmetastasis group than that in nonmetastasis group( p ＜ 0.01 ). (2)The intensity of CD15 expression was significantly higher in PCa than that in BPH. The upregnlation of the CD15 correlated with the rise of tumor Gleason grade and clinical stage( p＜0.01 ). The intensity of CD15 expression was significantly higher in metastasis group than in nonmetastasis group. (3)There was significant reverse correlation between the expression level of CD44s and CD15 (r = -0.785, p＜0.001). Conclusions Aberrant expressions of CD44s and CD15 may be correlated with clinical stage, Gleason grade and metastasis in PCa. CD44s and CD15 play an important role in the malignant progression of PCa, and could be used as a marker of malignant potention and prognosis of patient with PCa.     

CD15s抗原在乳腺癌中的表达及意义

目的探讨CD15s抗原在乳腺癌组织中的表达及分布特点以及与肿瘤发生、发展和转移的关系.方法应用催化信号放大系统免疫组化技术,对乳腺癌及正常乳腺组织进行CD15s抗原检测,采用胶体金免疫电镜技术观察超微结构组织中CD15s抗原的分布特征.结果 94例乳腺癌中75例CD15s表达呈阳性,表达阳性率为79.8%,10例正常乳腺组织腺管上皮细胞CD15s表达呈弱阳性.组织学分级为Ⅱ～Ⅲ级的乳腺癌中CD15s表达阳性率为87.3%,显著高于Ⅰ级的表达阳性率(69.2%,P＜0.05); 在伴有淋巴结转移的乳腺癌组织中,CD15s表达阳性率为90.2%,显著高于无淋巴结转移组的CD15s表达阳性率(67.4%,P＜0.05).免疫电镜显示,CD15s抗原主要分布于乳腺癌细胞质的界膜、内质网、高尔基体及近细胞核膜处和正常乳腺组织腺管上皮细胞质的界膜处.结论 CD15s对判断乳腺癌恶性程度和预测淋巴结转移状况是一个具有实用价值的标志物,并为探讨乳腺癌的发生、发展机理提供了一个新途径.     

CD15和CD15s在大肠癌中的表达及其与浸润转移的关系

目的:研究CD15和CD15s在大肠癌组织中表达状况及其与肿瘤发生、分化、转移及预后的关系.方法:应用催化信号放大法(CSA)检测90例大肠癌、51例转移淋巴结和30例远离癌组织的正常大肠粘膜CD15和CD15s的表达状况,并对其中53例患者进行随访.结果:CD15和CD15s阳性物质在远离癌组织的正常大肠粘膜中阳性表达率分别为93.3%和16.7%,而在大肠癌组织中阳性率分别为90.0%和94.4%,转移淋巴结标本中阳性率分别为68.7%和96.1%.结论:癌细胞膜Lewis糖抗原的唾液酸化及异常糖基的形成,可能与肿瘤转移行为有关.CD15s可以作为预测大肠癌侵袭、转移的肿瘤相关抗原,对评估患者预后较CD15具有更重要的临床意义.     

胆囊癌组织中CD15抗原的定量研究

目的 探讨ＣＤ１５抗原的表达及含量与胆囊癌的临床病理意义。方法 应用微波－ＬＳＡＢ免疫组织化学法和图像分析技术，检测４５例胆囊癌、１７例胆囊腺瘤和１０他蚀性胆囊炎组织中ＣＤ１５抗原表达及含量。结果 ＣＤ１５例表达阳性率及阳性细胞积分光密度值，在胆囊癌中显著高于胆囊腺瘤和慢性胆炎（Ｐ〈０．０５，Ｐ〈０．０１），胆囊癌Ⅱ、Ⅲ级显著高于１级（Ｐ〈０．０５，Ｐ〈０．０１）；有转移的肿瘤显著高于无转移的肿瘤     

CD15抗原和CD44V6蛋白在乳腺癌中的表达及意义

细胞黏附分子(cellular adhesion molecules,CAMs)是一大类位于细胞表面的糖蛋白分子,在恶性肿瘤的发生发展过程大多伴有CAMs或其配基的变化,CD15抗原和CD44V6蛋白均为其家族成员,它们在乳腺良恶性病变中有不同表达。本研究通过检测CD15抗原和CD44V6蛋白在乳腺癌及乳腺增生组织     

CD15抗原和bcl-2基因蛋白在甲状腺癌中的表达及相关性研究

目的 研究甲状腺癌组织中ＣＤ１５抗原和ｂｃｌ－２基因蛋白的表达与肿瘤发生和转移的关系,并探讨ＣＤ１５与ｂｃｌ－２基因蛋白表达的关系。方法 应用微波－ＬＳＡＢ免疫组织化学法检测５０例甲状腺癌、４５例甲状腺腺瘤和２０例癌旁正常甲状腺组织中ＣＤ１５和ｂｃｌ－２基因蛋白表达。结果 在甲状腺癌组织中ＣＤ１５和ｂｃｌ－２基因蛋白表达阳性率分别为６８．０％和４６．０％,均显著高于甲状腺腺瘤和癌旁正常甲状腺组织（     

食管癌组织中CD15抗原和组织蛋白酶D的表达及其意义

目的;探讨CD15抗原和组织蛋白酶D(Cath-D)在食管癌的表达意义及其相互关系。方法:应用微波-LSAB免疫组化法,探讨65例食管癌组织中CD15和Cath-D的表达阳性率及其相互关系。结果:食管癌CD15和Cath-D阳性率分别为58.5％(38/65)和64.6％(42/65)。CD15和Cath-D表达均与肿瘤分级、浸润、淋巴结转移和预后呈显著有关(P均<0.05)。CD15表达与Cath-D表达相关联。结论:食管癌CD15和Cath-D表达对判断其恶性程度、预测其侵袭转移趋势和预后及指导治疗有重要意义。     

肿瘤-睾丸抗原在胃癌组织中的表达

我们对MAGE 1、MAGE 3、BAGE、LAGE、NY ESO 1、SCP 1、SSX 2和SSX 4这 8种肿瘤 睾丸抗原 (CTA)在胃癌组织及其相应正常组织中的表达进行检测 ,旨在为特异性免疫治疗在胃癌中的应用提供依据。一、对象与方法1.组织标本 :42例胃癌组织标本取自上海瑞金医院普外科 2 0 0 3年 5月～2 0 0 3年 10月间手术治疗胃癌患者 ,同时取距离肿瘤边缘 5cm以上癌旁正常组织作为对照。标本经手术切除后立即放入液氮中 ,3 0min后保存于 -80℃直至RNA抽提。所有胃癌组织及相应正常组织均经术后病理证实。其中 ,男 2 8例 ,女 14例 ,低分化腺癌 2…     

肾移植前后外周血淋巴细胞CD15S抗原表达的临床意义

我们应用流式细胞技术检测了肾移植前后不同情况下外周血淋巴细胞表面CD15S抗原的表达 ,并结合临床对各组间的异同进行探讨。现报告如下。材料与方法　本组 12 1例。男 96例 ,女 2 5例。年龄 19～ 70岁 ,平均 32 .8岁。分为 6组。 5组随机选自行血液透析的尿毒症患者及肾移植术后 2个月内的患者。尿毒症群体反应性抗体 (PRA)阴性组 (B组 ) 11例 ,PRA <10 %。PRA阳性组 (C组 ) 10例 ,PRA 10 %～ 70 %。术后稳定组 (D组 ) 4 8例 ,为肾移植后尿量正常 ,血清肌酐水平稳定在正常范围1周以上的患者。急性排斥反应组 (E组 ) 30例 ,临床表现…     

CD9 expression in gastric cancer and its significance

BACKGROUND: The tetraspanin transmembrane protein CD9 is known to be involved in cell adhesion, proliferation, and cell motility. Previous studies have reported that reduced expression of CD9 is related to aggressive behavior of cancer cells. However, the cause-and-effect relationship between the CD9 expression level and the state of malignancy remains unclear. Here, we investigated the connection between the CD9 expression level and the state of malignancy in gastric cancers. MATERIALS AND METHODS: The expression of CD9 was examined in primary and metastatic gastric carcinoma tissues. In total, specimens from 78 patients were used for immunohistological staining and specimens from 57 patients were subjected to Northern blotting. Paired samples of tumor/normal tissues obtained from five cases of gastric cancer were used for Western blotting. RESULTS: CD9 expression was observed at both the message level and the protein level in primary gastric carcinoma tissues, lymph node metastatic tissues, and peritoneal dissemination tissues. Contrary to previous reports for other cancers, CD9 expression was intensified in cancerous areas of gastric cancers in comparison with noncancerous areas in the same patient. When analyzed by the malignancy status based on the clinicopathological diagnosis, there was a tendency that CD9 expression was observed in severe vessel invasion, active lymph node metastasis, and advanced stage. CONCLUSIONS: CD9 expression was rather intensified in gastric cancer tissue in comparison with normal tissues. CD9 expression was more prominent in advanced gastric cancer.     

胃癌PCNA和CD44V6表达的相互关系及其临床意义的研究

目的　研究增殖细胞核抗原 (PCNA)和CD44V6表达与胃癌侵袭转移及预后的关系 ,探讨胃癌组织PCNA和CD44V6表达的相互关系。方法　采用SP免疫组化染色方法 ,检测 90例胃癌组织PCNA和CD44V6的表达情况。结果　胃癌组织PCNA标记指数 (LI)为6 3 83 %± 17 16 % ,CD44V6阳性表达率为 74 4% (6 7 90 ) ;PCNALI和CD44V6表达强度与胃癌淋巴结转移、浸润深度及TNM分期均呈显著正相关 (P <0 .0 5 ) ;PCNALI≥ 5 0 %或CD44V6强阳性表达的胃癌患者术后 1,3 ,5年生存率均显著降低 (P <0 .0 5 ) ;CD44V6阳性表达胃癌组织的PCNALI显著高于CD44V6阴性表达者 (P <0 .0 5 )。结论　PCNA和CD44V6表达与胃癌侵袭转移及预后显著相关 ;CD44V6的表达可能有助于胃癌增殖活性的增加     

S-Tn抗原在胃癌及胃癌前病变组织中表达的研究

目的　探讨S Tn抗原在胃癌及胃癌前病变组织中的表达情况 ,评价S Tn抗原对胃癌的诊断价值。方法　采用免疫组织化学染色法对 10 9例胃癌、70例癌旁粘膜、19例慢性萎缩性胃炎(chronicatrophicgastritis,CAG)、2例慢性浅表性胃炎 (chronicsuperficialgastritis,CSG)、2 8例正常胃粘膜进行染色分析。结果　胃癌组总阳性率为 74 3% ,其中印戒细胞癌阳性率最高为 83 8% ,随着分化程度的降低 ,阳性率愈来愈低。癌旁粘膜阳性率为 84 3% ,明显高于胃癌组 (P <0 0 1)。CAG组阳性率为 73 7% ,CAG伴肠上皮化生 (intestinalmetaplasia,IM)组明显高于CAG伴不典型增生 (atypicalprolif eration ,ATP)组 (P <0 0 1)。CSG组及正常粘膜为阴性。结论　S Tn在胃癌、癌旁粘膜和萎缩性胃炎中均有较高表达 ,其中以癌旁粘膜组表达率最高。伴有IM时S Tn表达更高 ,为 91 6 %。     

Immunoreactive carcinoembryonic antigen in gastric juice in patients with gastric cancer

To clarify the significance of immunoreactive carcinoembryonic antigen in gastric juice (gastric CEA) from gastric cancer patients, we studied the gastric CEA in comparison with cancer progress, histologic types of tumors, staining, for CEA and extent of intestinal metaplasia. The gastric CEA levels from patients with other gastric diseases, i.e., gastric ulcer, duodenal ulcer and chronic gastritis were also investigated. The mean gastric CEA level from gastric cancer patients was significantly higher than from gastric or duodenal ulcer patients, but there was no significant difference between levels in patients with gastric cancer and in those with chronic gastritis. The gastric CEA levels from cancer patients increased with progression of the cancer. The differentiated carcinomas showed significantly higher gastric CEA levels than the poorly differentiated carcinomas. Positive CEA tumors showed significantly higher gastric CEA levels than did the negative CEA tumors. The tumors with diffuse intestinal metaplasia revealed high gastric CEA levels. Determination of gastric CEA levels is considered to be useful for screening of gastric cancer.     

Immunoreactive carcinoembryonic antigen in gastric juice in patients with gastric cancer

To clarify the significance of immunoreactive carcinoembryonic antigen in gastric juice (gastric CEA) from gastric cancer patients, we studied the gastric CEA in comparison with cancer progress, histologic types of tumors, staining for CEA and extent of intestinal metaplasia. The gastric CEA levels from patients with other gastric diseases, i.e., gastric ulcer, duodenal ulcer and chronic gastritis were also investigated. The mean gastric CEA level from gastric cancer patients was significantly higher than from gastric or duodenal ulcer patients, but there was no significant difference between levels in patients with gastric cancer and in those with chronic gastritis. The gastric CEA levels from cancer patients increased with progression of the cancer. The differentiated carcinomas showed significantly higher gastric CEA levels than the poorly differentiated carcinomas. Positive CEA tumors showed significantly higher gastric CEA levels than did the negative CEA tumors. The tumors with diffuse intestinal metaplasia revealed high gastric CEA levels. Determination of gastric CEA levels is considered to be useful for screening of gastric cancer.     

Changes in serum and tissue carcinoembryonic antigen with growth of human gastric cancer xenografts in nude mice

Four human gastric cancer xenografts in nude mice were analyzed for tumor growth and associated levels of carcinoembryonic antigen (CEA) in sera and tissues. The xenografts were established from gastric cancer patients and transplanted serially over 20 passages in our laboratory. Histologically, two xenografts were intestinal type and the other two were diffuse type. All of them were positive for CEA immunohistochemically. Individual xenografts showed a positive correlation between tumor growth and serum CEA level, with correlation coefficients ranging from 0.73 to 0.91. Serum CEA levels rose continuously with increasing tumor weight after inoculation. A positive correlation was also observed between the tissue CEA level and tumor growth, the former increasing along with the latter, showing the correlation coefficients ranging from 0.69 to 0.81. Furthermore, the level of serum CEA closely paralleled to that of tissue CEA, showing the correlation coefficients ranged 0.54 to 0.90. The deviation of the ratio of serum CEA level/tissue CEA level was constant among the xenografts, although the mean ratio differed slightly. These results suggest that serum CEA level is closely correlated with tissue CEA level as well as tumor growth, and that the elevation of serum CEA is attributable to the tissue CEA level rather than tumor weight.     

CD44剪接变异体6在胃癌的表达及意义

目的 探讨ＣＤ４４Ｖ６表达与胃癌生物学行为及转移的关系。方法 应用免疫组织化学方法检测ＣＤ４４Ｖ６在２０５例胃癌组织中的表达。结果 胃癌ＣＤ４４Ｖ６表达阳性率为４７．３％。分化型型胃癌的Ｖ６阳性率为６７．４％，未分化型胃癌的阳笥率为３２．８％，两者差异有显著性。Ｖ６的表达在静脉侵犯 ５３．６％，较无静脉侵犯组为高。肝转移组的Ｖ６阳性率为７０．２％，明显高于无肝转移组。结论ＣＤ４４Ｖ６对胃癌的血源性     

胃癌组织中P15蛋白和血管内皮生长因子表达及其相关性的研究

目的　研究胃癌组织中P15蛋白和血管内皮生长因子 (VEGF)的表达及相关性。方法　应用SP免疫组织化学方法检测了 16 0例胃癌和 5 2例胃良性病变中P15蛋白和VEGF的表达情况。结果　P15蛋白在胃癌中阳性率为 43 8% (70 /16 0例 ) ,在胃良性病变中的阳性率为 6 9 2 % (36 /5 2例 ) (P <0 0 5 )。VEGF在胃癌中的阳性表达率为 75 0 % (12 0 /16 0例 ) ,在胃良性病变中的阳性表达率为 7 7% (4/5 2例 ) (P <0 0 0 1)。P15蛋白阳性 /VEGF阴性表达率于胃癌中为 11 3% (18/16 0例 ) ,胃良性病变中为 6 9 2 % (36 /5 2例 ) (P <0 0 0 1) ;P15蛋白阴性 /VEGF阳性表达率于胃癌中为 42 5 % (6 8/16 0例 ) ,胃良性病变中为 7 7% (4/5 2例 ) (P <0 0 0 1)。结论　胃癌中存在P15蛋白下调和VEGF上调 ,且两者具有相关性。