99例继发性闭经患者性激素六项分析

女性继发性闭经是妇女常见临床症状之一,对家庭和社会有着重要影响.本文对99例继发性闭经患者进行性激素六项水平分析:睾酮(T)、雌二醇(E2)、促黄体生成素(LH)、促卵泡激素(FSH)、泌乳素(PRL)、孕酮(P),旨在探讨性激素水平变化与女性继发性闭经的关系,为诊断闭经原因与指导治疗提供参考依据.     

男性肺癌患者血清性激素测定及其意义

近几年，性激素、促性腺激素与各种疾病的关系日益受到医学者的重视。为了解男性肺癌患者血清中雌二醇(E2)、泌乳素(PRL)，促黄体生成素(LH)及促卵泡生成素(FSH)的含量及相互之间的关系，我们应用RIA法测定了47例男性肺癌和20例正常男性血清中E2、PRL、LH及FSH的含量，现将检测结果报告如下。     

注射大剂量促黄体激素释放激素类似物后受孕大鼠垂体促性腺激素细胞的免疫组织化学变化

本文用特异的抗鼠促黄体激素(抗LH)、抗鼠卵泡刺激素(抗FSH)和抗人绒毛膜促性腺激素(hCG)血清,以PAP免疫组织化学方法,观察注射大剂量促黄体激素释放激素类似物(LHRH-A)后受孕大鼠垂体促性腺激素细胞的变化。注射后24 h,促性腺激素细胞的免疫组织化学反应明显减弱;注射后48 h,促性腺激素细胞的免疫组织化学反应开始恢复;注射后168 h,其恢复过程仍在进行。计数对照组和实验组不同免疫反应强度的LH细胞数和它们占LH细胞总数的百分数,并用统计学方法检验,发现其结果与上述一致。大剂量LHRH-A能刺激受孕大鼠垂体促性腺激素细胞释放大量LH,从而抑制卵巢合成孕酮的功能,并导致终止妊娠。本文支持这种看法,而且认为,这种对促性腺激素细胞分泌活动的影响是可以恢复的。     

溴隐亭对高泌乳素血症妇女促性腺激素诱导排卵的影响效果研究

目的 探讨溴隐亭对高泌乳素血症妇女促性腺激素诱导排卵的影响效果。方法 选取2015年6月~2016年6月我院接受治疗的60例高泌乳素血症患者,随机分为对照组和观察组各30例。对照组采用促性腺激素治疗,观察组在对照组基础上采用溴隐亭治疗,观察两组患者的治疗效果,LH（黄体生成激素）、FSH（促卵泡生长激素）、E2（雌二醇）、PRL（泌乳素）、促排卵周期数和不良反应发生率。结果 观察组患者的总有效率（93.33%）优于对照组（70.00%）,LH、FSH、LH/FSH、睾酮和胰岛素水平改善情况好于对照组,不良反应发生率（20.00%）低于对照组（3.33%）,促排卵周期数少于对照组,两组患者的治疗效果,LH、FSH、E2和PRL水平,促排卵周期数和不良反应发生率相比,差异具有统计学意义（P＜0.05）。结论 采用促性腺激素联合溴隐亭治疗高泌乳素血症患者具有较大的意义,值得推广应用。     

促卵泡激素受体结合抑制剂抑制小鼠卵巢发育

目的探讨不同浓度的促卵泡激素受体结合抑制剂(FRBI)对小鼠卵巢发育、生殖激素分泌及其蛋白表达的影响。方法给FRBI组小鼠分别肌肉注射20、30和40 mg/kg FRBI;促卵泡激素(FSH)组注射10 IU/kg FSH;对照组(CG)注射0.2 mL 0.9%氯化钠溶液,每组均连续注射5 d。精确称量每只小鼠体质量和双侧卵巢质量;测定卵巢组织切片中皮质厚度(OCT)和次级卵泡形态大小;ELISA测定血清FSH浓度;Western blot检测卵巢FSH受体(FSHR)表达。结果实验第15天起,FRBI组小鼠卵巢质量低于CG和FSH组(P0.05),以FRBI-30 mg组小鼠卵巢质量最低;FRBI组OCT、次级卵泡最大纵径(MLD)和最大横径(MTD)低于CG组和FSH组(P0.05),FRBI-40 mg组小鼠卵巢的MLD和MTD值最低;第15天时,FRBI-30 mg组小鼠血清FSH的浓度最低;随着FRBI注射剂量的增加,卵巢中FSHR表达水平逐渐降低,FRBI组的FSHR蛋白水平明显低于FSH组(P0.05)。结论 FRBI能抑制卵巢和卵泡发育,降低血清FSH浓度,下调卵巢FSHR蛋白表达水平。     

闭经妇女血液流变学变化与性激素关系的探讨

对高促性腺激素组(高Gn)32例,低促性腺激素组(低Gn)27例,分别测定血液流变学及性激素.结果:两组全血低切粘度(ηb40~(s-1))、血浆粘度(ηp)、还原粘度(ηr)、k值、体外血栓长度(L)、湿重(MW)与对照组比较均有非常显著差异.高Gn组促卵泡成熟激素(FSH)、黄体生成激素(LH)均显著增高,低Gn组FSH、LH均显著下降.两组雌二醇(E_2)催乳素(PRL)均下降,睾酮(T)变化不大.指出高Gn、低Gn闭经妇女尽管闭经的原因不同,但最终均可造成体内E_2水平低落及血液流变学高粘性的改变.提示:对闭经妇女存在的高粘血症应引起临床重视,且血液流变学检查为其早期诊断及疗效的观察提供了客观依据.     

正常男性血清生殖内分泌激素与睾丸、精液的相关研究

激素是协调机体不同代谢作用的化学信使。生殖内分泌激素与男性的睾丸发育、精子发生及性功能密切相关[1] 。下丘脑分泌促性腺激素释放激素 (GnRH)作用于垂体前叶分泌促性腺激素 ,包括促卵泡成熟激素 (FSH)、黄体生成素 (LH)和垂体泌乳素 (PRL)。FSH作用于睾丸支持细胞产生抑制素B ,参与FSH的反馈调节 ,LH则作用于睾丸间质细胞产生睾酮(T)。曲细精管和男性附属性腺的功能是受雄激素调节 ,这些激素的分泌同时受多种因素影响。为了探讨正常男性血清中生殖内分泌激素的水平及其与相关因素的关系 ,我们从 2 0 0 0年 1～ 12月对 5 2 3名…     

促卵泡素受体基因突变造成遗传性促性腺机能亢进卵巢发育不全

正常性腺功能有赖于垂体-性腺轴的完整性。两个垂体促性腺激素——黄体生成素(LH)与促卵泡素(FSH)在这个调节回路中起重要作用。到青春期前的卵巢中,滤泡发育到成熟前停止,造成滤泡闭锁。到青春期,由于促性腺激素刺激导致滤泡成熟和排卵。促性腺激素分泌不足或卵巢对促性腺激素刺激缺乏反应都会造成青春期卵巢停止发育。当卵巢     

性早熟女童血清性激素,IGF-Ⅰ,Leptin含量及其意义

目的 :探讨血清雌二醇 (E2 )、促黄体生成素 (LH)、促卵泡刺激素 (FSH)、胰岛素样生长因子Ⅰ (IGF -Ⅰ )、瘦素 (Leptin)水平在女童特发中枢性性早熟 (ICPP)诊断中的价值。方法 :采用化学发光免疫分析 (CLIA)和放射免疫分析 (RIA)测定 35例以乳房早发育为主诉的性早熟女童血清E2 、LH、FSH、IGF -Ⅰ、Leptin水平。结果 :ICPP组血清E2 、LH、FSH、IGF -Ⅰ、Leptin水平明显高于SPT组 ,且差异有显著性 (P <0 .0 5 ,P <0 .0 1或P <0 .0 0 1 ) ,以基础LH/FSH >0 .2为阳性界限 ,用于诊断ICPP的阳性率为 86 6 % (1 3/ 1 5 ) ,SPT的阳性率为 2 0 % (4 /2 0 )。血清IGF -Ⅰ水平与E2 水平呈正相关 (r =0 .4 7,P <0 .0 1 )。血清Leptin水平与E2 、IGF -Ⅰ水平不相关。结论 :测定血清E2 、LH、FSH、IGF -Ⅰ、Leptin水平对ICPP早期诊断有较好的价值     

继发性闭经患者性激素测定的临床意义

为探讨性激素水平与继发性闭经的关系,以及在临床诊断和治疗中的意义,我们对178例继发性闭经患者进行性激素五项水平分析:泌乳素(PRL)、促卵泡激素(FSH)、促黄体生成素(LH)、雌二醇(E2)、睾酮(T)。现将结果报告如下。1资料和方法1.1临床资料1.1.1病例组自2002年～2004年到我院就诊的继发性闭经患者178例,年龄(20～39)岁,平均(26.3±5.5)岁,抽血检测前2个月未用任何影响激素水平的药物。1.1.2对照组同时期40名健康女性,年龄(20～39)岁,平均(27.1±5.23)岁,2月内未用任何影响激素水平药物。1.2方法采集被检测者早晨空腹静脉血3ml(对照组…     

Depersonalization,adversity, emotionality,and coping with stressful situations

Depersonalization is defined as persistent or recurrent episodes of feeling detached or estranged from a sense of self and the world. This study addressed the primary question: Do nonclinical individuals who endorse high symptomatic depersonalization have inherently more intense emotional responses, along with more childhood adversity and past trauma? In this IRB approved study, participants who met clinical levels of depersonalization (n = 43, 16.3%) were compared to a group without clinical levels of depersonalization (n = 221, 83.7%). Adverse childhood experiences, adult traumatic events, emotional overexcitability, coping strategies under stress, and anxiety were examined in both groups. The variables to assess depersonalization severity included the Dissociative Experience Scale-II, Cambridge Depersonalization Scale, and Multiscale Dissociation Inventory. The results indicated that clinical levels of depersonalization were identified in 16.3% of the sample. The high depersonalization group had significantly more adverse childhood experiences, in particular, emotional abuse and neglect. They also experienced more adult traumatic events, higher levels of anxiety, more emotional overexcitability, and they employed a less adaptive emotion-oriented coping strategy under stress. It is recommended that treating depersonalization symptoms should include examining childhood adversity, especially emotional abuse and neglect. Based on study findings, emotion regulation skills should be promoted to help individuals with elevated depersonalization manage their emotion-oriented coping strategies, anxiety, and emotional overexcitability.     

Comparison of CLA with BPT, SPT, and RAST in children with asthma

Bronchial provocation tests (BPT) with allergen extracts were performed in 82 children with asthma, and the results were compared with the chemiluminescent assay (CLA), the radio-allergo-sorbent-test (RAST) and skin prick test (SPT). It was found that CLA matched BPT with a concordance of 87% (77-93%), RAST with a concordance of 74% (64-83%) and SPT with 80% (70-88%). Most often, CLA and RAST gave similar results, although their classes did not match precisely.     

Osteochondrodysplasia with rhizomelia, platyspondyly, callosal agenesis, thrombocytopenia, hydrocephalus, and hypertension

A female infant born at term to phenotypically normal nonconsanguinous parents had hypertension, thrombocytopenia, hydrocephalus, callosal agenesis, and nonlethal rhizomelic osteochondrodysplasia. Her osteochondrodysplasia was characterized roentgenographically by shortening and metaphyseal broadening of long bones, without bowing, and by platyspondyly, with deficient ossification of dorsal and central portions of vertebral bodies. By light microscopy, the iliac crest growth plate showed expansion of the zone of chondrocyte hypertrophy and degeneration, with faulty columnar alignment, sparse vascular ingrowth, and irregular mineralization at the zone of chondroosseous transformation. These findings appear to define a novel osteochondrodysplasia, which in association with hypertension, thrombocytopenia, hydrocephalus, and callosal agenesis may constitute a new syndrome.     

Facts, fallacies, fears, and frustrations with human pheromones

Among primates in general, pheromones are of variable importance to social communication. Data on humans have generated the greatest controversy regarding the existence of pheromonal communication. In this review, the likelihood of pheromonal communication in humans is assessed with a discussion of chemical compounds produced by the axilla that may function as pheromones; the likelihood that the vomeronasal organ (VNO), a putative pheromone receptor organ in many other mammals, is functional in humans; and the possible ways pheromones operate in humans. In the human axilla, the interactions between the cutaneous microflora and axillary secretions render this region analogous to scent glands found in other primates. Both the chemistry of axillary secretions and their effects on conspecifics in humans appear to be analogous to other mammalian pheromone systems. Whichever chemical compounds serve a pheromonal function in humans, another unknown is the receptor. Although the VNO has been implicated in the reception of pheromones in many vertebrates, it is not the only pathway through which such information has access to the central nervous system; there is ample evidence to support the view that the olfactory epithelium can respond to pheromones. Furthermore, if a chemical activates receptors within the VNO, this does not necessarily mean that the compound is a pheromone. An important caveat for humans is that critical components typically found within the functioning VNO of other, nonprimate, mammals are lacking, suggesting that the human VNO does not function in the way that has been described for other mammals. In a broader perspective, pheromones can be classified as primers, signalers, modulators, and releasers. There is good evidence to support the presence of the former three in humans. Examples include affects on the menstrual cycle (primer effects); olfactory recognition of newborn by its mother (signaler); individuals may exude different odors based on mood (suggestive of modulator effects). However, there is no good evidence for releaser effects in adult humans. It is emphasized that no bioassay-guided study has led to the isolation of true human pheromones, a step that will elucidate specific functions to human chemical signals.     

Sibs with growth deficiency, delayed bone age, congenital hip dislocation, and iridocorneal abnormalities with glaucoma

We report on a brother and sister with short stature, delayed bone age, developmental delay, congenital hip dislocation, and iridocorneal abnormalities with onset of glaucoma at or soon after birth. Results of endocrine evaluation were normal. To our knowledge, no similar pattern of defects has been reported previously.     

Infection with hepatitis A, B, C, and delta viruses among patients with acute hepatitis in Mongolia

One hundred ten consecutive patients (60 males and 50 females; age, mean +/- standard deviation [SD], 22.6 +/- 6.4 years; range 16-48 years) who were clinically diagnosed with sporadic acute hepatitis between December 2004 and January 2005 in Ulaanbaatar, Mongolia, were studied. IgM antibodies to hepatitis A virus were detected in 18 patients (16.4%), IgM antibodies to hepatitis B core (anti-HBc IgM) in 38 patients (34.5%) including two patients with concurrent hepatitis delta virus (HDV) infection, and hepatitis C virus RNA in nine patients (8.2%). There were 30 hepatitis B virus (HBV) carriers who had detectable hepatitis B surface antigen and antibodies to HDV but were negative for anti-HBc IgM, suggesting that they acquired type D acute hepatitis due to superinfection of HDV on a background of chronic HBV infection. None had IgM antibodies to hepatitis E virus (HEV). Consequently, 16.4, 32.7, 6.4, 1.8, and 27.3% of the patients were diagnosed as having acute hepatitis of type A, B, C, type B + D (HBV/HDV coinfection), and type D (superinfection of HDV), respectively. The cause of hepatitis was not known in the remaining 17 patients (15.5%). All 18 HAV isolates were genotyped as IA, all 9 HCV isolates were genotyped as 1b, and all 32 HDV isolates were classified into genotype I. The distribution of HBV genotypes among the 67 HBV isolates was A (1.5%, n = 1) and D (98.5%, n = 66). The present study indicates that de novo infections of HAV, HBV, HCV, and HDV are prevalent among young adults in Mongolia.