土壤微量元素背景值与癌症关系的主成分分析

目的：探讨土壤微量元素含量与癌症发病率间的关系。方法：测定癌症高发区江苏省扬中市土壤中Cu、Pb、Zn、Ni、Cr、As、Se、Co、V、Mn、F等11种微量元素的含量，应用主成分分析法提取出贡献率大的主成分与1991—1999年癌症发病率做多重回归分析。结果：土壤11种微量元素间存在较强相关性，应用主成分分析法提取出第一主成分与癌症发病率拟合线性回归方程，显示土壤微量元素含量与胃癌、肝癌及全部癌发病率均呈负相关，其中Se元素作用最强。结论：土壤微量元素，特别是Se元素的含量可能在癌症发生中起重要作用。     

鼻咽癌患者放射治疗前后头发微量元素的测定

为了探讨鼻咽癌患者放射治疗前后机体微量元素含量的变化情况,作者应用电感耦合等离子体原子发射光谱(ICP-AES)法及2,3-二氨基萘荧光法测定鼻咽癌患者放射治疗前后头发52例的微量元素含量,并与正常人头发63例的微量元素含量作对比。结果表明:鼻咽癌患者放疗前头发中Zn、Fe和Se含量低于正常人,经放疗后,头发中Zn、Fe、Se和Cr均高于放疗前,且放疗后Zn和Se含量接近正常人。     

肝癌患者,肝癌高发家族成员及各组健康人群头发中微量元素的含量分析

采用原子吸收分光光度计法,测定了37例肝癌,38例肝癌高发家族或非癌家族成员以及118例包括扶绥肝癌高、低发区和南宁市的健康正常人发中8种微量元素的含量。发现:在扶绥肝瘸低、高发区居民和肝癌患者三组人群中,Cu、Cu/Mo值有依次增高而Mn、Mo、Se、Se/Cu值依次下降的趋向,肝癌高发区居民发中微量元素谱的变化,与肝癌患者有很相似的特征。作者认为,Cu、Mn、Mo、Se等元素的丰缺,很可能与扶绥居民肝癌高发有关。     

食管黏膜内微量元素含量与癌基因突变关系的研究

目的 :探讨食管黏膜内微量元素含量、基因突变与食管病变的关系。方法 :15 1例食管活检标本分为正常、慢性炎、非典型增生与早期癌四种类型 ,以X 射线能谱仪扫描电镜进行微量元素定量检测 ,以S P免疫组化法检测PCNA表达及p5 3突变状态。结果 :Zn、Se、Mo随着食管上皮增生程度的升级含量渐减 ,不同组织学类型间差异有显著性 (P <0 .0 5 ) ,Cu Zn、Ni随着食管病变程度的升级含量渐增 ,差异有显著性 (P <0 .0 5 ) ,p5 3、PCNA阳性表达在正常上皮与非典型增生及早期癌组间差异有极显著性 (P <0 .0 1)。结论 :Zn、Se、Mo、Cu、Ni含量变化可能为高发区食管上皮p5 3突变与PCNA过表达的始动因素     

硒——抗癌之星

硒是人和动物的必需微量元素之一。硒对维持人体的正常生理功能,保护人体的健康起着重要的作用。据大量的临床资料显示,癌症患者血液中的硒含量明显低于正常人,血硒含量高的人群,其癌症的发病率低。     

结直肠癌组织微量元素的分析电镜研究

目的：探讨结、直肠癌组织中的微量元素变化。方法；应用分析电镜对37例结、直肠癌，移行粘膜和正常粘膜组织中元素进行研究。结果；在结、直肠癌中磷元素和钾元素相对含量显著高于正常粘膜（P＜0．05）。结论：上述结果提示在结、直肠癌中除微量元素钙、铜、锌和锰含量降低以外，元素磷和钾含量增加。     

白血病与微量元素──江西省资料分析

白血病与微量元素──江西省资料分析曾昭华，曾雪萍，蔡伟娣土壤的元素及其含量直接影响作物及地下水的元素及其含量，它们的分布及含量具有一致性。而且不同岩类地区生长的人体中也具有不同的元素及含量。如人体血液中化学元素的丰度与地壳中元素的丰度有惊人的一致关系...     

微量元素锰和癌的关系

锰(Mn)是人体必需的微量元素,大量的资料证明Mn与多种癌的发病有关,本文就Mn与癌的有关问题综述于后。 一、癌症高发区环境Mn含量变化及其意义 国内外资料表明,缺Mn地区癌肿的发病率高。苏联阿拉木图省及芬兰上壤中含＊n量高的地区,癌肿发病率均低,认为Mn有抗癌作用”‘。顺德是广东肝癌高发区,据广东省地质局’“对顺德地质环境的调查结果表明,微量元素Mn变化规律明显,重病区土壤Mn (571 39PPm)低于轻病区(69.7lpPm),重病区井水Mn(0.19PPm)低于轻病区 门.00PPm),都具有非常显著性差异(P。0.of),并与肝癌死亡率呈     

胃癌、不典型增生和正常人血清中视黄醇、α-生育酚、β-胡萝卜素和12种元素的研究

本文测定了45例胃癌、41例胃不典型增生(胃癌癌前病变)和48例对照组血清中视黄醇、α-生育酚、β-胡萝卜素和12种元素(Zn、Cu、Mn、Fe、Ca、Mg、Ni、Cr、Co、Cd、Mo、Se)的含量。结果表明:胃癌病人血清中的β-胡萝卜素和硒、钴含量低于胃不典型增生病人,更低于对照组。此外,胃癌组与胃不典型增生组比较,明显降低的元素有镍、铬;明显升高的元素有锰、钙、镉。胃癌组与对照组比较明显降低的有锌、铁、铬、镉;明显升高的有锰、钙、镁、钼。胃不典型增生组与对照组比较,明显下降的有锰、铁、镉;明显升高的有钼。上述15个指标,经逐步回归判别分析,筛选出10个变量指标(Mn、Fe、Ca、Cr、Mo、Co、Cd、Se、α-生育酚、β-胡萝卜素)用以识别胃癌,其识别符合率为100％。若用逐步回归判别分析,判别血清中Mn、Cr、Mo、Co、Cd五个微量元素的含量,其识别胃癌的符合率亦为97.8％。作者认为对患者血清中微量元素进行逐步回归判别可作为胃癌诊断的参考指标,并认为适当补充硒和β-胡萝卜素将有助于预防胃癌。     

微波消解-ICP-MS法测定肺癌患者体液和头发中的14种元素的含量

背景与目的 体液和组织中的微量元素的含量是多种疾病的重要监测指标,对肺癌病人体液和头发 中的微量元素进行分析,有助于肺癌的早期诊断及治疗效果评价.本研究旨在发展一种能够测定肺癌患者体液和毛 发中Cr、Fe、Mn、Al、Cd、Cu、Zn、Ni、Se、Pb、Ca、Mg、Sr、P等14种微量元素的含量的方法.方法 采用微波 消解法对取自16例肺癌患者的48份样品进行消解,利用ICP-MS方法进行元素含量测定.结果 利用GBW09101人发成 分分析标准物质对所建方法的准确性进行了考察,结果表明,测得值与标准值之间不存在统计学差异.利用所建的 方法分析了来自16例肺癌患者的48份样品,从中发现了若干规律.结论 本方法适用于肺癌患者体液和毛发中微量 元素的测定,对于肺癌的诊断和治疗具有一定的临床意义.     

食管癌高低发区饮用水微量元素谱的多元分析

背景与目的： 研究食管癌高低发区饮用水中微量元素谱(Trace elements,TE),为食管癌(Esophageal cancer,EC)病因提供研究依据。材料与方法：采集食管癌高低发地区的饮用水样,用高频电感耦合等离子体发射光谱法(Inductively coupled plasma_atomic emission spectgrometry,ICP_AES)测定13种元素(Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Se, Sr, V, Zn)含量,并进行t检验、相关分析、建立回归方程。 结果： 检测发现食管癌高低发区的水样中的微量元素谱不同,Fisher判别法,其识别准确率为93％。 结论： 不同环境水中微量元素对食管癌发生率有影响。     

启东肝癌与微量元素初步研究

启东是肝癌高发区,其水、土中铜、锌、锰、镍、钴、钼等微量元素富集。粮中铜、锌含量偏高,而硒、钼含量显著降低,与肝癌发病率呈负相关。肝癌高发区居民血液中铜、锌、锰、钴、铅等水平高于低发区,但血硒、钼水平却相反,显著低于低发区。肝癌高发区居民头发中铜、锌、锰均高于低发区,但钼却相反,低于低发区。肝癌惠者头发中铜比自然人群显著增加,但锰却相反。值得注意的是肝癌组织中缺钼或少钼。以上结果表明,肝癌与硒、钼密切相关,而与锰、镍等微量元素的关系有待深入探索。     

急性白血病患者血清微量元素谱分析

采用ＤＣＰ－ＡＥＳ检测了４０例急性白血病（初发１８例，化疗后２２例）血清中锌、铜、铁、硒、镍、钛等１５种元素含量。结果显示：初发急性白血病患者血清锌、铁、硒、镍、钛均降低，铜、钙、镁、铜／锌比值均升高。化疗达ＣＲ后，血清锌、铜、铜／锌、硒均可正常，而化疗后ＮＲ组上述元素无改变。血清钙、镍、钛化疗后ＣＲ和ＮＲ组均无改善。表明微量元素与急性白血病转归相关。     

Research on serum levels of retinol, α-tocopherol β-carotene,and 12 elements in gastric dysplasia and gastric cancer patients

Serum levers of retinol, α-tocopherol, β-carotene and elements (Zn, Cu, Mn, Ca, Mg, Cr, Co, Cd, Mo, Se) were determined in 45 gastric cancer and 41 gastric dysplasia patients, and 48 normal subjects. The results showed that β-carotene and Se, Co were lower in gastric cancer patients than that in gastric dysplasia patients or in normal subjects, Ni and Cr levels were lower and Mn, Ca and Cd were higher in gastric cancer than in dysplasia patients. Zn, Fe, Cr, Cd were lower and Mn, Ca, Mg, Mo levels were higher in gastric cancer patients than in normal. The differences mentioned are statistically significant. The stepwise discriminant analysis of 10 variables (Mn, Fe, Ca, Cr, Mo, Co, Cd, Se, α-tocophrol, β-carotene) were used in identifying gastric cancer, with 100% of the positive rate. The potential protective effect of β-carotene and Se against gastric cancer is an interesting postulate. We suggest that optimum supplement of β-carotene and Se might will be beneficial to gastric dysplasia patients in preventing the development of gastric cancer.     

Trace elemental analysis of normal, benign hypertrophic and cancerous tissues of the prostate gland using the particle-induced X-ray emission technique.

Trace elemental analysis was carried out in the tissue samples of normal, benign hypertrophic and carcinoma prostate using the particle-induced X-ray emission technique. A proton beam of 3 MeV energy was used to excite the samples. The elements Cl, K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Se, and Br were identified and their concentrations were estimated. It is observed that in benign tissues the concentrations of Cl, K, Zn, and Se are lower (P<0.05) and those of Cr, Fe, Ni, and Cu are higher (P<0.05 ) than in normal tissues. The concentrations of K, Ca, Zn, Se, and Br are lower (P<0.01) and those of Ti, Cr, Mn, Fe, Ni, and Cu are significantly higher (P<0.0005) in cancerous tissues than in normal tissues. Free radicals generated by elevated levels of Cr, Fe, Ni, and Cu possibly initiate and promote prostate cancer by oxidative DNA damage. The excess Cu levels in cancerous tissues support the fact that Cu promotes cancer through angiogenesis. The higher levels of Fe observed in cancerous tissues might be a consequence of tumor growth through angiogenesis. Significantly higher levels of Ni and Cr observed in carcinoma tissues support the well-established role of Ni and Cr as carcinogens. It is likely that the observed low levels of Zn and Se in cancerous tissues lead to the development of prostate cancer owing to a decrease in antioxidative defense capacity and impaired immune function of cells and also suggest that the inability to retain high levels of Zn and Se may possibly be an important factor in the development and progression of malignant prostate cells. In order to substantiate the observed elevated or deficient levels of trace elements in initiating, promoting, and inhibiting prostate cancer, several cellular and molecular studies are required.     

包头地区乳腺肿瘤及其复发转移的血清微量元素变化研究

目的探讨9种微量元素与乳腺肿瘤的关系。方法采用原子吸收分光度计对81例健康女性志愿者、94例乳腺良性肿瘤患者、93例乳腺癌患者手术前后和38例乳腺癌复发转移患者血清中Fe、Zn、CU、Mn、Pb、Cd、Cr、Ni、Mo的含量进行测定。结果血清Fe、Zn、Cu、Mn、Pb随乳腺肿瘤病情加重呈明显升高趋势,与乳腺癌呈正相关,而Mo随病情的加重明显降低,与乳腺癌呈负相关。结论有6种微量元素与乳腺肿瘤的发病有显著关系,其中Fe、Zn、Cu、Mn、Pb可能是乳腺癌的危险因素,而Mo为乳腺癌的保护因素。     

Pattern recognition on the trace element spectrum in gastric cancer and peptic ulcer

The trace element spectrum of gastric tissue and whole blood specimens from gastric cancer and peptic ulcer patients was recognized with pattern recognition technique in order to obtain the chemical information of gastric cancer. Thirty specimens were obtained from cancer patients in each of the 58 gastric tissues and 54 whole blood specimens. Five pathological types were divided into two groups: peptic ulcer and dysplasia as the ulcer group; well-differentiated, undifferentiated and mucinous carcinoma as the cancer group. The accuracy of recognizing the several spectra was 90%: nine trace element spectra (Cu, Zn, Ni, Co, Cr, Cd, Mn, Fe and Mg) for the tissue specimens from the cancer and ulcer groups; six (Cu, Zn, Mn, Co, Cr and Cd) for dysplasia and well-differentiated adenocarcinoma tissues; five (Cu, Ni, Cr, Cd and Fe) for the whole blood specimens from these two groups and six (Cu, Zn, Cr, Cd, Fe and Ni) for the whole blood specimens from dysplasia and peptic ulcer (including 10 normal individuals). The data were treated by non-linear mapping method and calculated by TRS-80 III type microcomputer.     

Some Mineral,trace Element and Heavy Metal Concentrations in Lung Cancer

Objective: We aimed to determine the relationship between some mineral, trace element and heavy metallevels in the patients of lung cancer by measuring serum levels of copper (Cu), lead (Pb), zinc (Zn), iron (Fe),cobalt (Co), cadmium (Cd), manganese (Mn), magnesium (Mg). Methods: A total of 50 lung cancer and humanhealth (30 lung cancer and 20 healthy human) were included in the study. Venous blood samples of each lungcancer were obtained, and serum Cu, Pb, Zn, Fe, Cd, Co, Mn, Mg levels were analysed by Atomic AbsorptionSpectrophotometer measurements. Results: Mg value measured in lung cancer group were lower than the controlgroup and this was statistically significant (P<0.01). Serum Cu level was significantly lower with lung cancercompared to healthy human (P<0.01). Pb level was significantly higher than those of controls (P<0.01). Theserum Zn level was significantly lower in serum of lung cancer group than controls (P<0.01). Serum Mn and Colevels were found increased in lung cancer group than controls (P<0.01). Cd value was higher in lung cancer butit was not statistically significant (p>0.01). The mean concentration of Fe in the serum of lung cancer patientswas higher than in the controls, but the difference was not significant (p>0.01). There was a positive correlationbetween Cd and Pb level, and between Mn and Fe levels in lung cancer. There was a negative correlation betweenCo and Zn levels of healthy human. There was a negative correlation between Co and Mg levels of lung cancer.Conclusions: Serum Cu, Pb, Zn, Fe, Mg, Co, Mn and Cd might be play a role in the patients of lung cancers.Zn may protective as potent lung cancer. In addition, it is suggested that low levels of zinc can induce thepathogenesis of lung cancer.     

人体正常和良恶性组织中8种微量元素的分析

本文报告人体恶性(63例)、良性(64例)和正常(65例)3种组织中8种微量元素测量结呆。证明恶性组织中铜、镍明显高于正常和良性组织,又以胃肠癌显著。栖前者低于后二者,而两组癌无差异。恶性组锌、锰明显低于正常组。其他癌锌降低显著,且锌有恶性<良性<正常的趋势。初步探讨3种组织中微量元素的变化规律和临床意义。     

重金属对秀丽线虫毒性排序的Meta回归分析

目的： 基于已发表的重金属对秀丽线虫毒性作用的文献,在排除其他影响因素的前提下,综合分析和比较重金属对秀丽线虫的毒性作用大小。方法：利用Scan It软件提取了20篇国内外相关文献中以图形表达的不同重金属对秀丽线虫不同指标的毒性数据,将相同指标进行汇总,以毒性指标终点为因变量,金属种类、浓度、暴露时间、发育期等为自变量,进行Meta回归模型分析。结果：各金属对秀丽线虫死亡率影响大小次序为：汞 (mercury,Hg)＞铜 (copper,Cu)＞铅 (lead,Pb)＞铬 (chromium,Cr)＞ 铝 (aluminum,Al)＞镉(cadmiun,Cd)＞钴(cobalt,Co)＞镍(nickel,Ni)＞锌(zinc,Zn);对体长的影响大小次序为：Pb＞银 (silver,Ag) ＞Hg＞Co＞Al＞Cr＞Zn＞钙 (calcium,Ca)＞Ni;对世代时间的影响大小次序为：Ni＞Al＞Co＞Ag＞Cr＞Zn＞Cu＞Pb; 对头部摆动频率的影响大小次序为：Hg＞Pb＞Ni＞Ag＞Cu＞Zn＞Cr＞Co＞Cd＞Al。结论：Meta回归模型可用于分析不同重金属的研究,并对重金属毒性进行有效排序,但有些浓度下的毒性仍需要结合实验进行综合分析和验证。