促红细胞生成素基因修饰骨髓间充质干细胞移植治疗大鼠脑梗死

背景：促红细胞生成素具有神经元的保护及促进神经再生的作用。目的：观察促红细胞生成素修饰的骨髓间充质干细胞尾静脉移植对大鼠脑梗死的治疗效果。方法：用Western blot鉴定外源人促红细胞生成素基因在骨髓间充质干细胞中的表达。采用线栓法建立大鼠大脑中动脉阻塞模型,模型组尾静脉注射PBS、骨髓间充质干细胞组注射骨髓间充质干细胞悬液,促红细胞生成素-骨髓间充质干细胞组注射转染了促红细胞生成素的骨髓间充质干细胞悬液。移植后3 d及移植后1,2,3,4 周行改良神经功能评分,检测神经功能的损伤情况。移植后4 周将大鼠麻醉后断头取脑,RT-PCR检测脑组织中bcl-2/bax基因表达变化,用原位末端标记法测定细胞凋亡情况、苏木精-伊红染色及荧光显微镜观察PKH26标记的骨髓间充质干细胞的存活和分布情况。结果与结论：Western blot结果显示,转染人促红细胞生成素基因的骨髓间充质干细胞体外能表达促红细胞生成素蛋白。移植后1-4周,骨髓间充质干细胞组和促红细胞生成素-骨髓间充质干细胞组神经缺损评分明显低于模型组(P < 0.05,P < 0.01)。与骨髓间充质干细胞组及模型组相比,大鼠脑梗死区组织促红细胞生成素-骨髓间充质干细胞组bcl-2基因的表达明显增高(P < 0.05),bax基因的表达明显降低(P < 0.05),凋亡细胞明显减少,PKH26阳性细胞数明显增多(P < 0.05)。结果证实,促红细胞生成素修饰的骨髓间充质干细胞尾静脉移植对脑梗死大鼠脑梗死疗效较好。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

神经干细胞移植联合氨基甲酰促红细胞生成素对脑瘫大鼠的作用及机制

目的研究神经干细胞（NSCs）移植联合氨基甲酰促红细胞生成素（CEPO）对新生脑瘫大鼠脑损伤的保护作用。方法从孕14～16天大鼠获得神经干细胞,经过培养并传代后备用。利用7日龄wistar大鼠制作脑瘫模型,给予CEPO腹腔注射和神经干细胞移植,对神经干细胞移植后的分化、凋亡情况,免疫分子在移植前后的改变,大鼠神经系统功能检测等各项指标进行评估,并与单-神经干细胞移植、CEPO腹腔注射效果进行比较。结果缺氧缺血脑损伤后,IFN-γ和IL-1β的浓度显著提高（P〈0．05）,MHC的含量增加,大鼠学习能力下降（P〈0．05）。联合应用CEPO和神经干细胞移植可使移植细胞存活率提高并向神经元分化,降低血中IFN-γ和IL-1β水平,调节MHC抗原的表达,进而明显改善大鼠的神经运动功能。结论应用CEPO可促进移植细胞存活,调节免疫分子的分泌,改善细胞存活的环境,对移植细胞具有明显的保护作用。     

重组人促红细胞生成素联合骨髓间充质干细胞移植治疗脓毒症大鼠心肌损伤

背景：相关研究表明促红细胞生成素及骨髓间充质干细胞均对心肌凋亡有一定影响,但两者联合应用治疗脓毒症相关性心肌损伤少见报道。 目的：观察骨髓间充质干细胞移植联合腹腔内注射促红细胞生成素对脓毒症大鼠心肌细胞病理学改变及细胞凋亡的影响。 方法：选用SD大鼠50只,随机数字表法均分为5组(n=10),应用盲肠结扎穿孔术建立脓毒症大鼠模型。骨髓间充质干细胞组建模后即刻尾静脉输注异体间充质干细胞;促红细胞生成素组建模后即刻腹腔内注射促红细胞生成素;促红细胞生成素+细胞移植组两者联合应用;模型组行盲肠结扎穿孔术,对照组开腹后不做任何处理,均尾静脉输注相同容量的生理盐水。24 h后麻醉处死实验动物,取心肌标本,采用苏木精-伊红染色观察心肌组织形态;用Western blot方法检测心肌组织中凋亡蛋白Bax、Caspase-3,抗凋亡蛋白Bcl-2的表达量。 结果与结论：苏木精-伊红染色结果：对照组可见心肌细胞排列整齐,心肌细胞结构完整;模型组可见广泛心肌纤维断裂,排列紊乱,心肌细胞肿胀或皱缩,可见空泡变性;心肌间质血管充血、水肿,炎症细胞浸润;促红细胞生成素组与骨髓间充质干细胞组心肌组织相似,炎性细胞浸润情况较轻,期间散在分布正常心肌细胞;促红细胞生成素+细胞移植组心肌细胞损害较轻,间质充血不明显,少量炎症细胞浸润。Western blot结果：促红细胞生成素+细胞移植组Bcl-2蛋白表达显著高于促红细胞生成素组、模型组及对照组(P < 0.01),Bax及Caspase-3蛋白表达量均减低(P < 0.05)。结果显示,骨髓间充质干细胞移植联合给予促红细胞生成素在脓毒症相关性心肌损伤的治疗中可减轻心肌的病理学改变、抑制心肌细胞的凋亡,其机制可能是通过上调抗凋亡蛋白、下调凋亡蛋白的表达来实现的。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

人促红细胞生成素基因修饰人羊膜间充质干细胞蛛网膜下腔移植对脑损伤的影响

BACKGROUND:Studies have shown that as a regulator of bone marrow function erythropoietin is a glycoprotein that controls the development of the central nervous system and has neurotrophic and neuroprotective potential. Therefore, transplantation of human amniotic mesenchymal stem cells genetically modified by human erythropoietin is a new choice for brain injury treatment. OBJECTIVE:To observe the effect of transplantation of human amniotic mesenchymal stem cells genetically modified by human erythropoietin on the functional recovery from brain injury in rats. METHODS:Eukaryotic expression plasmid pcDNA3.1 carrying erythropoietin was successfully constructed and transferred into amniotic mesenchymal stem cells cultured in vitro. Expression of erythropoietin was detected using western blot assay before and after transfection. Rat models of middle cerebral arterial occlusion was made and given transplantation of transfected amniotic mesenchymal stem cells via the tail vein (transfection group). Additionally, model and simple cell transplantation groups were set in a comparative study. RESULTS AND CONCLUSION:Findings from western blot detection showed that transfected cells could express human erythropoietin. Compared with the other groups, modified neurologic severity scores, growth-associated protein 43 and aquaporin 9 at mRNA and protein levels were all decreased significantly in the transfection group. Furthermore, the number of cells positive for CM-Dil was highest in the transfection group, followed by simple cell transplantation group, and lowest in the model group (all P < 0.05). Overall findings from this study show that human erythropoietin-modified human amniotic mesenchymal stem cell transplantation promotes neurologic recovery from brain injury through eliciting a reduction in growth-associated protein 43 and aquaporin 9 at mRNA and protein levels as well as inhibiting cell apoptosis.     

促红细胞生成素促进脊髓损伤大鼠移植神经干细胞存活和迁移

背景：如何有效促进移植入脊髓损伤组织内的神经干细胞存活和迁移,是目前神经修复研究的重点。 目的：观察促红细胞生成素对脊髓损伤大鼠移植神经干细胞存活、增殖和迁移的影响。 方法：将60只SD大鼠随机分为3组,均制备脊髓横断损伤模型。造模7 d,神经干细胞移植组和促红细胞生成素组于脊髓损伤处移植BrdU标记的神经干细胞7 μL(1×10⁹ L^-1),脊髓损伤对照组移植DMEM/F12培养基;促红细胞生成素组腹腔内注射促红细胞生成素5 000 U/kg,1次/d,连续注射7 d,其余两组注射等量生理盐水。于细胞移植后8周取损伤脊髓组织。 结果与结论：造模2周后,神经干细胞移植组和促红细胞生成素组BBB评分明显高于脊髓损伤对照组(P < 0.05),造模4周后,促红细胞生成素组BBB评分明显高于神经干细胞移植组(P < 0.05)。免疫荧光染色显示促红细胞生成素组大鼠损伤脊髓组织BrdU阳性细胞数量及迁移距离均大于神经干细胞移植组(P < 0.05)。说明促红细胞生成素能促进损伤脊髓组织原位移植的神经干细胞的存活与迁移,加速神经功能修复。     

自体骨髓单个核细胞移植治疗下肢动脉闭塞症

背景：下肢缺血性疾病治疗中血管再生技术已成为研究的热点。 目的：观察自体骨髓单个核细胞移植后下肢缺血性疾病患者肢体缺血症状的改善情况。 方法：东南大学医学院附属江阴医院胸心血管外科及南京大学医学院附属南京市鼓楼医院血管外科于2007年10月至2011年10月采用自体骨髓单个核细胞治疗35例下肢缺血性疾病患者,抽取自体骨髓单个核细胞,沿下肢动脉走行路径进行多点注射,测定患肢疼痛程度、冷感缓解程度、踝肱指数、经皮氧分压及皮肤溃疡或坏疽面积的改变,动脉造影观察血管新生情况。 结果与结论：①疼痛评分：骨髓单个核细胞移植后2个月和1年的疼痛评分均较移植前有下降趋势,差异有显著性意义(P < 0.05)。移植后2个月与移植后1年评分比较,差异无显著性意义(P > 0.05)。②冷感缓解程度：移植后2个月缓解率为51%,移植后1年缓解率为60%,与移植前比较差异有显著性意义(P < 0.05)。③踝肱指数的变化：移植2个月踝肱指数均较移植前有升高趋势,但差异无显著性意义 (P > 0.05),移植后1年踝肱指数较移植前有明显增高,差异有显著性意义(P < 0.05)。④经皮氧分压的变化：对经皮氧分压增加值进行比较,移植后2个月及移植后1年均较移植前明显增加,差异有显著性意义(P < 0.05),移植后1年与移植后2个月比较差异无显著性意义(P > 0.05)。⑤行走距离：移植后2个月和移植后1年的行走距离明显增加,与移植前比较差异有显著性意义(P < 0.05),移植后1年与移植后2个月比较差异无显著性意义(P > 0.05)。⑥溃疡创面的变化：移植后2个月和1年的溃疡面积均较移植前有缩小趋势,差异有显著性意义(P < 0.05)。⑦新生血管评价：移植后股动脉造影显示缺血下肢注射骨髓单个核细胞的部位毛细血管增生明显,且与已有的毛细血管相互连接形成侧支循环,促进了缺血下肢的血液供应。     

人胚胎干细胞向内皮细胞的分化诱导

BACKGROUND:Human embryonic stem cells exhibit self-renewal and multi-differentiation potential, and can differentiate into endothelial cells under certain induction conditions. OBJECTIVE:To explore induced conditions of the human embryonic stem cells differentiating into endothelial cells and to investigate the effect of vascular endothelial growth factors on the endothelial differentiation of human embryonic stem cells. METHODS:After resuscitation, passage 40 human embryonic stem cell lines H9 were subjected to suspension culture to prepare embryos, and after 5-day culture, these cells were cultured in attachment medium to differentiate into embryoid bodies, followed by induction with 50 µg/L vascular endothelial growth factors. Passage 2 and 15 embryonic stem cells after induced differentiation were taken for Dil-Ac-LDL uptake test and immunohistochemical staining, respectively. RESULTS AND CONCLUSION:After 1-day culture, cord-like or polygonal monolayer cells around embryoid bodies showed bud-like and radial growth with a relative rapid speed merging into surrounding colonies; at 2-3 days, the number of suspension cells increased further, but the small-round cells in the center began to die; at 5 days, embryoid bodies started to passage, and aggregated cells exhibited typical paving stone-like appearance. Moreover, some human embryonic cells after induced differentiation could actively take up fluorescent labeled LDL, and red fluorescent particles appeared. Additionally, passage 15 embryonic stem cells after induced differentiation could express CD31 and FLK-1. These findings suggest that human embryonic stem cells induced by vascular endothelial growth factors can differentiate into endothelial cells.     

内皮祖细胞与高血压病的应用研究进展

BACKGROUND: Structural and functional changes of endothelial cells are the common pathological basis of cardiovascular disease. Severe structural and functional damage of endothelial cells are found in patients with hypertension or coronary heart diseases.     

脂肪源性干细胞体外分离培养及向内皮祖细胞的诱导分化

背景：脂肪源性干细胞是目前组织工程种子细胞的研究热点,通常采用胶原酶消化脂肪组织来获得,但是存在着操作繁琐,花费高等不足。 目的：观察组织块培养法分离的脂肪源性干细胞的基本生物学特性及其向骨细胞、脂肪细胞及内皮祖细胞的诱导分化潜能。 方法：无菌条件下切取大鼠双侧腹股沟脂肪组织,组织块法分离培养脂肪源性干细胞,CCK-8法检测细胞增殖活性,流式细胞仪分析干细胞表面标志表达。取第4代脂肪源性干细胞用成骨诱导液、成脂诱导液、内皮祖细胞诱导液培养两三周并进行鉴定。 结果与结论：组织块培养法得到的脂肪源性干细胞易扩增,传代后以长梭形细胞生长为主,呈漩涡状排列,克隆样生长;经多次传代仍能保持较强的增殖能力,细胞生长曲线呈抛物线形;细胞表面标志CD44、CD90、CD29呈阳性表达,CD45、CD31呈阴性表达。成脂诱导后细胞油红O染色阳性、成骨诱导后细胞茜素红染色阳性。诱导后的内皮祖细胞CD34及DiI-ac-LDL和FITC-UEA-1双荧光染色阳性。以上结果显示组织块培养法能成功分离培养出脂肪源性干细胞,且获得的细胞纯度较高、易于增殖培养,能高表达干细胞表面抗原以及具有向骨细胞、脂肪细胞及内皮祖细胞多向分化的潜能,可以满足组织工程研究种子细胞的需求。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

体外磁共振观察胎鼠神经干细胞的标记

背景：神经干细胞移植后的细胞存活、识别和迁移需动态监控。 目的：通过体外磁共振技术对胎鼠神经干细胞体外标记,为神经干细胞在神经系统修复中的应用提供依据。 方法：采用胎鼠神经干细胞的分离与培养标记、染色剂鉴定及神经干细胞的活性检测,构建大鼠脑缺血再灌注模型,采用超顺磁性氧化铁颗粒体外标记胎鼠的神经干细胞并移植至模型大鼠左侧脑内,未标记的胎鼠神经干细胞移植至右侧脑中,对标记的细胞进行普鲁士蓝染色,观察其定植和迁移情况,并通过磁共振示踪动态的监测神经干细胞在活体移植之后的信号改变情况。 结果与结论：超顺磁性氧化铁颗粒体外标记胎鼠神经干细胞的方法效率达95%以上,电镜结果显示超顺磁性氧化铁颗粒体外标记胎鼠的神经干细胞内含铁颗粒,且集中在溶酶体和内涵体当中,磁共振结果显示胎鼠标记的神经干细胞呈现低信号改变,细胞活性的影响与未标记组差异无显著性意义,但标记的胎鼠神经干细胞T2WI 与 T2*WI信号降低。证实超顺磁性氧化铁颗粒体外标记胎鼠的神经干细胞可高效表达,磁共振的监控可以用于神经干细胞的活体示踪。   中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

不同组织来源间充质干细胞向血管内皮细胞诱导分化的研究与进展

文章快速阅读：文题释义： 血管内皮细胞：亦称内皮细胞,是胚胎发育最早分化的组织,且属于中胚层,通常指在心、血管和淋巴管内表面的单层扁平上皮,其形成血管的内壁。血管内皮细胞具有吞噬异物、细菌和衰老及坏死组织的功能,还参与免疫活动。理论上间充质干细胞可以分化为血管内皮细胞。 诱导间充质干细胞分化为内皮细胞的主要因素：细胞分化是由许多分子信号通路调控的复杂过程,在细胞分化早期干细胞通过旁分泌作用释放信号蛋白分子,如血小板衍生生长因子、血管内皮生长因子A、转化生长因子β和Wnts等,刺激距离较远的细胞内的信号级联,使细胞定向分化。 摘要 背景：间充质干细胞可诱导分化为血管内皮细胞,在体外可形成血管移植物用于临床治疗缺血性疾病、血管组织工程及再生医学等领域。 目的：总结间充质干细胞的生物学特征,不同组织来源间充质干细胞诱导分化为血管内皮细胞的相关研究进展。 方法：文章由第一作者于2015年9至11月期间检索PubMed、Sciencedirect、Medline数据库2000年1月至2015年6月的相关文章,限定文章语言种类为English,检索词为“mesenchymal stem cells,vascular endothelial cell,cell differentiation”,初检文章156篇,筛选后对51篇文章进行分类总结。 结果与结论：①间充质干细胞来源广泛,其中骨髓来源研究最早最多,但随供者年龄增大,其增殖及分化能力随之减弱;②脐带、胎盘、羊膜都是产妇自体产物且分娩后会遗弃,故获取间充质干细胞比较容易,同时对新生儿和产妇不会造成任何痛苦和心理负担,受者更易于接受,不会引起道德伦理及法律方面的纠纷;③脐血来源充足,免疫原性较弱,且受胎盘屏障保护使其成分被病毒、细菌污染的概率低;④羊水因取材方式等限制其在临床应用。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程 ORCID: 0000-0002-2983-8929(陈国华)     

CD133+卵巢癌干细胞样细胞在血管内皮细胞中的分化

背景：大量研究证实,新生血管形成在肿瘤的生长、浸润以及转移过程中发挥重要作用。 目的：探讨CD133+卵巢癌干细胞样细胞向血管内皮细胞分化的特点。 方法：通过无血清培养方法从卵巢癌A2780细胞株中成功诱导出CD133⁺卵巢癌干细胞样细胞,在体外接种于铺或不铺Matrigel基质胶的96孔板内,观察不同时间点CD133⁺卵巢癌干细胞样细胞和人脐静脉内皮细胞形成管腔样结构能力。通过裸鼠皮下移植实验,免疫荧光法观察CD133⁺卵巢癌干细胞样细胞在卵巢癌血管新生中的作用。 结果与结论：CD133⁺卵巢癌干细胞样细胞和人脐静脉内皮细胞(阳性对照)在未铺 Matrigel基质胶上并不能形成相应的管腔结构,且不表达内皮细胞标志物CD31,在Matrigel基质胶上能够形成相对稳定的管腔结构,CD31表达明显。CD133⁺卵巢癌干细胞样细胞接种裸鼠皮下成瘤后,可观察到肿瘤组织中有人源性CD31的表达。结果表明CD133⁺卵巢癌干细胞样细胞能够分化为血管内皮细胞,参与肿瘤血管重建。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

人前脑啡肽基因修饰骨髓间充质干细胞系的生物学特性

BACKGROUND:Previous studies have found that the transplantation of modified cell lines exhibit analgesic effect. But little is reported on the biological characteristics of human preproenkephalin gene-modified bone marrow mesenchymal stem cell lines. OBJECTIVE:To observe the biological characteristics of human preproenkephalin gene-modified bone marrow mesenchymal stem cells. METHODS:Bone marrow mesenchymal stem cells were isolated and cultured to establish human preproenkephalin gene-modified bone marrow mesenchymal stem cell lines. RESULTS AND CONCLUSION:After freezing-thawing, passage 4 human bone marrow mesenchymal stem cells, human bone marrow mesenchymal stem cells-pBABE and human bone marrow mesenchymal stem cells-human preproenkephalin exhibited no significant changes in the cell viability (P > 0.05), as well as in the fat cell proportion after adipogenic induction (P > 0.05). Moreover, red calcium deposition was presented in all these cells by alizarin red staining after osteogenic induction. Flow cytometry results showed that passage 4 human bone marrow mesenchymal stem cells, human bone marrow mesenchymal stem cells-pBABE and human bone marrow mesenchymal stem cells-human preproenkephalin could express CD29 and CD44, but not express CD34 and CD45. Human preproenkephalin genes were highly expressed in human bone marrow mesenchymal stem cells-pBABE, but lowly expressed in passage 4 human bone marrow mesenchymal stem cells. Additionally, recombinant plasmid pBABE-preproenkephalin-modified human bone marrow mesenchymal stem cells could express enkephalin protein. In conclusion, human preproenkephalin gene-modified human bone marrow mesenchymal stem cell lines can maintain the pluripotent differentiation or proliferation capacity of bone marrow mesenchymal stem cells, and secrete enkephalin protein.     

血管内皮祖细胞改善骨质疏松大鼠骨髓间充质干细胞的增殖及凋亡

BACKGROUND: Previous studies have found that estrogen deficiency causes a reduction in the activity of bone marrow mesenchymal stem cells (BMSCs). OBJECTIVE: To explore the effect of endothelial progenitor cells (EPCs) on the BMSCs proliferation and apoptosis ability of osteoporosis rats. METHODS: Healthy female Sprague-Dawley rats, 6 weeks old, were enrolled and subjected to bilateral ovariectomy to make osteoporosis models. BMSCs and EPCs were isolated using density gradient centrifugation combined with adhesion method, and identified with surface markers, cell proliferation and immunocytochemistry in vitro. We used Transwell inserts to establish EPCs and OVX-BMSCs indirect co-culture system. Control groups were OVX-BMSCs group and sham-BMSCs group in which rats were only subjected to remove the equal amount of fat tissues around the ovary. Flow cytometry was applied to detect BMSCs proliferation and apoptosis ability. RESULTS AND CONCLUSION: Compared with the control groups, the results of flow cytometry test showed that the proportion of OVX-BMSCs at S phase was significantly increased at 3 days of indirect co-culture with EPCs and the apoptosis rate was significanty reduced at 10 days of indirect co-culture with EPCs (both P < 0.05). These results suggest that EPCs can promote the proliferation but inhibit the apoptosis of OVX- BMSCs. 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程      

雷帕霉素联合CD34抗体复合支架快速捕获外周血中内皮祖细胞

背景：临床用于治疗血管狭窄疾病的药物洗脱支架和单纯内皮修复型支架存在内皮化延迟和植入后再狭窄的问题。雷帕霉素联合CD34抗体复合支架可协同抵消抗增殖药物的内皮化延迟和内膜的过度增生,目前尚处于实验研究阶段。 目的：观察雷帕霉素联合CD34抗体复合支架捕获内皮祖细胞能力及其所捕获内皮祖细胞的分化特征。 方法：通过扫描电镜及间接免疫荧光观察雷帕霉素联合CD34抗体复合支架体外捕获外周血内皮祖细胞形态及其分化特征。通过荧光显微镜观察雷帕霉素联合CD34抗体复合支架植入兔耳动脉后捕获内皮祖细胞情况及支架片段的内皮化程度。 结果与结论：扫描电镜观察到CD34抗体涂层支架可捕获直径6-8 μm的纺锤状细胞,24 h时细胞变得充盈饱满。所捕获细胞具有内皮祖细胞的外形特征。间接免疫荧光观察CD34抗体支架表面可见大量血管内皮生长因子受体2阳性细胞黏附的红色荧光斑点。免疫荧光观察到CD34抗体涂层支架植入兔耳动脉24 h大部分被血管内皮细胞所覆盖,48 h达到完全覆盖,未见细胞异常聚集。结果表明雷帕霉素联合CD34抗体复合支架能特异性快速捕获外周血液中的血管内皮祖细胞,植入体内48 h即可完成血管内皮细胞覆盖,实现了支架快速内皮化,可促进内皮细胞修复。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

成人外侧盘状半月板损伤的有效影像学指标：MRI影像学评价

BACKGROUND: Currently, the morphologic changes of the discoid lateral meniscus (DLM) remain unclear, and morphological indexes used to evaluate the injuries of DLM still need to be explored. OBJECTIVE: To compare the MR imaging difference between symptomatic and asymptomatic DLM in adults, and to select reliable morphological parameters for assessing DLM. METHODS: A total of 36 patients (36 knees) with a symptomatic DLM (study group) and 34 patients (34 knees) with an asymptomatic DLM (control group) were included in this study. GE Healthcare Centricity RIS/PACKS System was used to measure the primary morphological parameters of two patients with DLM on coronal MR imaging. Intergroup comparison was performed with the Wilcoxon test. RESULTS AND CONCLUSION: On the coronal MR imaging, the width of the body portion and height of the free edge of the DLM was significantly increased in the study group compared with the control group (P < 0.001), while the height of the peripheral portion which connects with the capsule of the DLM was significantly decreased in the study group compared with the control group (P = 0.002). The height of the middle of the DLM and the width of the lateral femoral condyle showed no significant differences between the both groups (P > 0.05). The width ratio of the body of the DLM to the lateral femoral condyle and the height ratio of height of the free edge to the middle of the DLM were significantly increased in the study group compared with the control group (P < 0.001), while the height ratio of the peripheral portion to the middle for the DLM was significantly decreased compared with the control group (P < 0.001). These findings suggest that compared with asymptomatic discoid lateral meniscus, the width of the body portion and the height of the free edge for the DLM are increased; however, the height of the peripheral portion which connects with the capsule of the DLM is decreased. The width ratio of the body portion of the DLM to the lateral femoral condyle and the height ratio of the free edge to the middle for the DLM may be reliable morphological parameters for the assessment of the DLM in adults. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程