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1.
IL-6、IL-1和PDGF对大鼠肾小球系膜细胞生长的影响 总被引:2,自引:0,他引:2
目的:探讨白细胞介素-6(IL-6)、白细胞介素-1(IL-1)和血小板源生长因子(PDGF)对大鼠肾小球系膜细胞(MsC)增殖的影响。方法:采用改良的MTT法和BrdU掺入法检测SD大鼠体外MsC悬液中加入IL-6、IL-1及PDGF后12h及24h的增殖情况。分7组进行观察。结果:IL-6组、IL-1组和PDGF组12h及24h的光密度(OD值)与标记指数(LI)均为2%FCS组也均高,但所有实验组均不能达20%FCS组的细胞增殖水平。联合应用双因子刺激12h及24h均未见明显的协同促增殖作用。结论:IL-6、IL-1及PDGF均能在一定程度上刺激大鼠肾小球MsC增生。联合应用双因子刺激无明显的协同效应。 相似文献
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目的 研究核因子KB(NF-KB)在氧化低密度脂蛋白(Ox- LDL)诱导的体外培养的人肾小球系膜细胞表达单核/巨噬细胞趋化蛋白-1(MCP-1)中的作用。方法 采用凝胶迁移率变动分析检测NF-KB的DNA结合活性变化,以免疫组织化学观测细胞内p65的核转位,用细胞ELISA法检测细胞内 MCP-1及IKBα蛋白含量变化。结果 不同浓度(10、25、50、100μg/ml)Ox-LDL刺激肾小球系膜细胞均可引起细胞NF-KB的DNA结合活性增强、IKBα蛋白表达下降以及MCP-1蛋白表达增强,以50μg/ml刺激1h NF-KB活化及IKBα表达减弱最明显,作用24hMCP-1表达水平最高。NF-KB俯活化的同时伴有p65核转位。上述效应可被NF-KB特异性抑制剂吡咯二硫氨基甲酸酯(PDTC)所抑制。结论Ox-LDL刺激人肾小球系膜细胞产生MCP-1是由NF-KB调控,NF-KB参与了脂质肾损害的发病过程。 相似文献
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目的 观察CD28共刺激信号对淋巴细胞免疫活化基因表达的影响及环孢素A(CsA)对它们的抑制作用。方法 分离健康人外周血单个核细胞,分别给予抗CD3单克隆抗体(抗CD3mAb)单刺激或抗CD3mAb+抗CDW28单克隆抗体(抗CD28mAb)共刺激培养;加或不加入CsA。于培养后6h和24h收集细胞。以逆转录聚合酶链反应(RT-PCR)检测β-Actin、CD40L、bcl-xL、TGF-β1、F 相似文献
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转移生长因子—β,碱性成纤维细胞生长因子诱导人成骨细胞表达血小板衍生生 总被引:11,自引:3,他引:8
目的 探讨转移生长因子(TGF)-β、碱性成纤维细胞生长因子(bFGF)对人成骨细胞血小板衍生生长因子(PDGF)-BmRNA表达的影响及其意义。方法 体外分离培养人成骨细胞。在体外培养的成骨细胞中分别加入10、20、40、80、160ug/L梯度浓度的PDGF-BB培养细胞24h,以摄取^3H-TdR为细胞增殖指标检测细胞增殖状况。以4ug/L的TGF-β和10ug/L的bFGF培养细胞24h,寡核苷酸探针检测细胞PDGF-BmRNA的表达。结果 10~160ug/L的PDGF-BB可促进成骨细胞增殖(P〈0.05)。在普通培养条件下,细胞下表达PDGF-BmRNA;当培养体系中加入TGF-β和bFGF,可见PDGF-BmRNA的表达。结论 PDGF-B基因的表达可能是骨组织生长的储备因素,TGF-β和bRN 相似文献
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Rxa对L02细胞增殖的细胞周期分析和对Ki—67,CyclinD1,PCN … 总被引:1,自引:0,他引:1
探讨丹参提取物Rxa对正常肝细胞增殖速率的影响。方法应用流式细胞术分别测定L02细胞Rxa处理组和未处理组0、2、4、8、12小时G0-G1、S、G2-M期细胞指数,同步进行Ki-67、CyclinD1、PCNA蛋白表达的免疫组化分析。结果La细胞胞增殖速率较快,Ki-67抗原表达相对较强,CyclinD1、PCNA表砂相提前。结论Rxa可能促进L02细胞增殖。 相似文献
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为了进一步探索治疗膀胱癌的新途径,用人体脾脏组织制备粘附性淋巴因子激活的杀伤细胞(A-LAK细胞),通过生物素-亲和素系统将抗人膀胱癌单克隆抗体(BDI-1)与A-LAK细胞偶联构建出BDI-1-A-LAK细胞偶联物。采用4小时51Cr释放法测定BDI-1-A-LAK细胞偶联物体外对人膀胱癌BIU-87细胞的杀伤率,结果显示,扩增5天从每克人脾脏组织可制备得5.5×108个A-LAK细胞,BDI-1-A-LAK细胞偶联物对BIU-87细胞具有较强的特异性杀伤作用。提示BDI-1-A-LAK细胞偶联物可望用于膀胱癌导向治疗 相似文献
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微量元素对大鼠颅盖骨成骨细胞样细胞体外培养增殖分化的影响 总被引:8,自引:0,他引:8
本实验所用的细胞是按照Luben改良的方法获得,并用该技术及骨细胞培养物作为模型进行骨代谢中细胞增殖及调节机制的研究。使用3周龄SD大鼠颅盖骨,以胶原酶、胰蛋白酶消化收集细胞,接种在24孔培养板,每孔2×10 ̄6个细胞/ml,在10%胎牛血清的199培养液中于5%CO_2、37℃饱和湿度培养,在条件液中分别加10 ̄(-9)至10 ̄(-1)不同浓度的Zn ̄++、Cu ̄++、Mn ̄++、Mg ̄++、Ca ̄++微量元素培养,并从其形态学及生化指标探讨微量元素对成骨细胞的增殖、分化都有明显的影响。 相似文献
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抗骨质疏松药XW630对成骨细胞碱性磷酸酶mRNA表达的影响 总被引:4,自引:0,他引:4
目的研究新设计的抗骨质疏松药物XW630对成骨细胞碱性磷酸基因表达的影响,为其治疗骨代谢性疾病提供理论和实验依据。方法以成骨细胞MC3T3-E1为体外实验模型,用含有10^-6mol/L浓度的XW630,雌酚酮,四环素及四环素加雌酚酮的细胞培养液DMEM培养细胞,24h和72h提取细胞总RNA并与碱性磷酸酶cDNA进行Northern杂交。结果XW630对成骨细胞MC3T3-E1合成和分泌碱性磷酸 相似文献
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肿瘤坏死因子—α损伤的肺动脉内皮细胞对肺动脉平滑肌细胞增?… 总被引:2,自引:0,他引:2
目的 探讨肿瘤坏死因子-α(TNF-α)损伤的肺动脉内皮细胞对肺动脉平滑肌细胞(PASMC)增殖的影响及内皮细胞热应激反应的干扰作用。方法 以终浓度为500、1000、1500u/ml的TNF-孵育融合层的肺动脉内皮细胞或经过热应激反应的内皮细胞1h,然后用不含血清的DMEM培养基培养24h,收集上清培养液制成条件培养液(EC-CMⅠ、EC-CMⅡ)用于 孵育PASMC(Ⅰ组、Ⅱ组),同时用正常条 相似文献
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Uremic acquired cystic disease of kidney 总被引:1,自引:0,他引:1
I Ishikawa 《Urology》1985,26(2):101-108
Multiple cystic disease occurring in the diseased kidneys of patients with end-stage renal insufficiency is called uremic acquired cystic disease of the kidney. In male patients undergoing long-term hemodialysis the incidence of ACDK is markedly high. ACDK is known to be accompanied by tumor, bleeding, calculus, abscess, etc., and the complication of cancer of the kidney is a special problem. In patients undergoing hemodialysis, occurrence of ACDK, tumor, and kidney cancer are observed respectively at the rate of 47.1, 4.8, and 1.5 per cent. When hemodialysis patients show gross hematuria, flank pain, rapid decrease in hematocrit, and sustained fever, ACDK or its complications should be investigated. Since the risk accompanied by kidney cancer is high in spite of a lack of symptoms, regular screening by ultrasonic examination or CT scan is needed. Renal transplantation is also recommended because of the regression of ACDK after successful renal transplantation. In the future, it appears that ACDK should be considered one disease entity and added to the categories of renal cystic diseases. In addition, ACDK can be studied as a model for clarification of the mechanism of cyst and tumor occurrence. 相似文献
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Renal cell carcinoma in acquired cystic disease of the kidney manifested by spontaneous renal hemorrhage 总被引:1,自引:0,他引:1
Nishikawa Z Kataoka A Yuasa T Okamoto K Wakabayashi Y Yoshiki T Okada Y 《Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology》2000,91(12):727-730
Acquired cystic disease of the kidney (ACDK) is a common phenomenon in long-term adult dialysis patients with end-stage renal disease. Renal hemorrhage and neoplastic transformation of the cyst are two major complications of this entity and these two can occur independently. Here we describe a 65-year-old man with a history of hemodialysis-dependent end-stage renal failure for 12 years presented with macroscopic hematuria and right flank pain. Investigations revealed right massive perirenal and subcapsular hematoma with ACDK. The hemorrhagic state required nephrectomy of the right kidney. Histological study showed ACDK with massive subcapsular and perinephric hematoma containing minimal clear cell carcinoma region(0.5 cm in diameter). Although a causal relationship between renal hemorrhage and renal cell carcinoma in this patient was unproven, the present case suggests radical surgery can be a recommendable treatment modality of hemorrhagic ACDK. 相似文献
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In 1977, Dunnill et al. described a new disorder, bilateral multiple renal cystic disease. It occurred among hemodialysis patients whose original illness had not been cyst-related. Acquired cystic disease of the kidney (ACDK) is commonly observed in patients undergoing hemodialysis. The incidence of ACDK is 40-50% in reports of autopsy and surgical specimens, rising to more than 90% after 5-10 years of dialysis. The volume of the kidneys decreases in the first 3 years of dialysis and then increases as the rate of cyst formation increases. In male patients undergoing long-term hemodialysis the incidence of ACDK is markedly high. ACDK is also found in patients before hemodialysis. The primary concern in patients with ACDK is the increased incidence (5-19%) of renal cell carcinoma (RCC). The incidence is about twelve to eighteen times higher than that in the general population and the cancers may be asymptomatic. Therefore, screening is essential if carcinomas are to be detected early. Regular screening by ultrasonic examination or CT scan is needed. A patient requires nephrectomy when the kidney cancer exists or is suspected by dynamic CT scan. Nephrectomy is performed only on the side with renal mass. It has been argued that RCC associated with ACDK are innocuous and do not predispose the patient to an increased risk of death from RCC. RCC arising from ACDK is considered to be a tumor of low malignant potential, compared with classic RCC. However, RCC has been reported to metastasize in 16% of the patients on dialysis and to be the cause of death in 2% of the kidney transplant recipients. The etiology of ACDK is unclear and its incidence increases with the duration of dialysis. ACDK patients have a propensity to develop adenocarcinoma. The increased incidence of RCC in ACDK patients warrants careful radiologic monitoring of end-stage kidneys in selected patients. 相似文献
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K Fujita 《Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology》1990,81(8):1148-1154
The factors which made acquired cysts develop to renal tumors in chronic hemodialysis patients was investigated from clinical and histological findings. Although three renal tumors were found among 151 patients with chronic renal failure, clinically, one case had acquired cystic disease of the kidneys (ACDK), one case had simple cysts and the other case did not have any cyst. However, the last two cases had multiple small cysts histologically. In ACDK, renal tubules were degenerated and dilated, fused to each other, forming more larger cysts. Furthermore, epithelium of acquired cysts showed columer and dysplastic change, and papillary adenomas were developed into the inner space of the cyst wall. And ACDK kidney without renal tumor also revealed the same findings in histological examinations. It was postulated that renal tumor occurred from the papillary projections of the dysplastic epithelium of cysts. Therefore cysts in uremic patients seemed to be different from the simple cyst in normally functioning kidneys. 相似文献
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O Yokoyama K Miyazaki T Ishida Y Fujita R Miyazaki H Muramoto T Nakashima K Watanabe 《Hinyokika kiyo. Acta urologica Japonica》1991,37(2):107-112
Long-term dialysis patients frequently develop acquired cystic disease of the kidneys (ACDK). One hundred and sixty-seven dialysis patients were classified into two groups according to the presence or absence of ACDK, and the two groups were compared with regard to the dialysis period, laboratory findings, and so forth. Among the 167 dialysis patients, 37.7% showed ACDK and had an average dialysis duration of 10.3 years, which was significantly longer than the 5.0 years for patients without ACDK. The proportion of patients with ACDK rose with increasing duration of dialysis; 64.6% of the patients on dialysis for more than 10 years had ACDK. The values of hemoglobin and hematocrit of patients with ACDK were significantly higher than those without ACDK. Of 12 dialysis patients who were operated upon because of suspected renal tumor, pathological examination confirmed renal cell carcinoma in 8 and adenoma in one. One of these patients had been treated with chronic ambulatory peritoneal dialysis, and another had bilateral renal tumor. Among the 8 patients with renal cell carcinoma, ACDK was detected in 5 by means of CT and ultrasound tomography, and cystic changes were observed in two on light microscopic examination of the resected kidneys. Pathological examination of the non-tumorous renal tissues revealed hyperplastic or adenomatous changes in the cyst epithelium in 5 patients undergoing dialysis for more than 7.5 years. These changes were thought to be pre-neoplastic. The high incidence of ACDK and the development of renal tumors in long-term dialysis patients indicates the necessity of intensive monitoring. 相似文献
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目的:探讨后腹腔镜囊肿去顶减压术治疗肾脏囊性疾病的临床疗效及手术方法。方法:对57例肾脏囊性疾病患者行后腹腔镜囊肿去顶减压术,其中单纯性肾囊肿51例,多囊肾6例,回顾分析相关资料。结果:57例手术均获成功,无严重并发症发生。镜下操作时间单纯性肾囊肿15~35 min,多囊肾90~120 min;单纯性肾囊肿术中出血5~20 ml,多囊肾30~60 ml。术后随访3~24个月,单纯性囊肿无复发;多囊肾患者术后腰部胀痛缓解,3例合并肾功能不全的患者肾功能改善。结论:后腹腔镜囊肿去顶减压术治疗肾脏囊性疾病患者创伤小,康复快,疗效确切,可作为治疗肾脏囊性疾病的首选方法。 相似文献
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S Yamaguchi H Fujii S Kaneko S Yachiku T Anzai F Inada T Kobayashi K Furuta H Ishida 《Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology》1990,81(8):1183-1189
Ultrasonic examination of the kidney was performed on 280 patients undergoing chronic dialysis. Acquired cystic disease of the kidney (ACDK) was detected in 107 of 529 kidneys (20.2%). This paper presents an analysis of ultrasonotomograms of ACDK. Ultrasonic measurement of the size of ACDK was 72.5 +/- 15.2 mm in length and 41.7 +/- 9.8 mm in thickness. The size of ACDK was significantly greater than that of contracted kidneys by ultrasonographic diagnosis. With regard to sex distinction the length and thickness of ACDK were significantly greater in males than in females. As for laboratory data, patients with ACDK showed significantly higher values of red blood cell count, hematocrit and serum creatinine concentration compared with contracted kidneys. Prolongation of the dialysis peirod increased the incidence of ACDK. The size of ACDK showed a tendency to increase with duration of dialysis. However, no correlation was noted statistically between the incidence of ACDK and duration of dialysis and between the size of ACDK and duration of dialysis. There was a significantly lower incidence of ACDK in patients with diabetic nephropathy than those with chronic glomerulonephritis. A sonographic feature of ACDK is irregularity of the renal contour because of cystic transformation. Renal imaging, identification of the corticomedullary border, identification of the central echoes and increased parenchymal echogenicity were similar to other dialyzed kidneys. The main complications of ACDK are hemorrhage and tumor formation. We observed two retroperitoneal hematomas and one renal cell carcinoma developed within two years after this examination. The incidence of complications of ACDK was 5.1 per cent. We believe that patients with ACDK should be watched carefully by regular ultrasonic examination for early diagnosis and treatment of these complications. 相似文献
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Nakatani T Uchida J Iwai T Yoshida N Kuratsukuri K Takemoto Y Sugimura K 《Nephrology (Carlton, Vic.)》2003,8(2):101-103
SUMMARY: A 54-year-old-female patient who had received regular haemodialysis therapy for 12 years was referred to our hospital for evaluation of a left renal mass. Imaging examinations revealed acquired cystic disease of the kidney (ACDK) and a tumour-like lesion in the left kidney. Because of the preoperative diagnosis of the left renal cell carcinoma, the patient underwent a left nephrectomy. Pathological examination revealed xanthogranulomatous pyelonephritis. It was difficult to distinguish xanthogranulomatous pyelonephritis from renal cell carcinoma in our case, because it is very rare for xanthogranulomatous pyelonephritis to occur in ACDK. 相似文献
20.
Acquired cystic disease of kidney associated with renal cell carcinoma in chronic dialysis patients. 总被引:1,自引:0,他引:1
We describe 2 cases of acquired cystic disease of the kidney (ACDK) associated with renal cell carcinoma in patients treated with long-term hemodialysis. Both patients have had dialysis for five and eight years, respectively. Renal cell carcinomas of these patients are small, averaging 2 cm in diameter. They are clear cell type. Atypical epithelial hyperplasia arising from cystic areas can be seen intermingling with carcinoma. This confirms that atypical epithelial hyperplasia is a precursor of renal cell carcinoma. Although the incidence of renal cell carcinoma arising in ACDK is on the rise, the issue of how to manage patients with ACDK remained unsettled and required further study. 相似文献